Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 11 de 11
Filtrar
1.
J Exp Bot ; 70(21): 6203-6214, 2019 11 18.
Artículo en Inglés | MEDLINE | ID: mdl-31504781

RESUMEN

Leaf senescence is a form of developmentally programmed cell death that allows the remobilization of nutrients and cellular materials from leaves to sink tissues and organs. Among the catabolic reactions that occur upon senescence, little is known about the role of proline catabolism. In this study, the involvement in dark-induced senescence of proline dehydrogenases (ProDHs), which catalyse the first and rate-limiting step of proline oxidation in mitochondria, was investigated using prodh single- and double-mutants with the help of biochemical, proteomic, and metabolomic approaches. The presence of ProDH2 in mitochondria was confirmed by mass spectrometry and immunogold labelling in dark-induced leaves of Arabidopsis. The prodh1 prodh2 mutant exhibited enhanced levels of most tricarboxylic acid cycle intermediates and free amino acids, demonstrating a role of ProDH in mitochondrial metabolism. We also found evidence of the involvement and the importance of ProDH in respiration, with proline as an alternative substrate, and in remobilization of proline during senescence to generate glutamate and energy that can then be exported to sink tissues and organs.


Asunto(s)
Arabidopsis/metabolismo , Oscuridad , Mitocondrias/metabolismo , Hojas de la Planta/crecimiento & desarrollo , Hojas de la Planta/metabolismo , Prolina/metabolismo , Secuencia de Aminoácidos , Arabidopsis/genética , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Respiración de la Célula , Clorofila/metabolismo , Regulación de la Expresión Génica de las Plantas , Metaboloma , Mitocondrias/ultraestructura , Oxidación-Reducción , Consumo de Oxígeno
2.
Biochem J ; 473(17): 2623-34, 2016 09 01.
Artículo en Inglés | MEDLINE | ID: mdl-27303048

RESUMEN

Proline accumulates in many plant species in response to environmental stresses. Upon relief from stress, proline is rapidly oxidized in mitochondria by proline dehydrogenase (ProDH) and then by pyrroline-5-carboxylate dehydrogenase (P5CDH). Two ProDH genes have been identified in the genome of the model plant Arabidopsis thaliana To gain a better understanding of ProDH1 functions in mitochondria, proteomic analysis was performed. ProDH1 polypeptides were identified in Arabidopsis mitochondria by immunoblotting gels after 2D blue native (BN)-SDS/PAGE, probing them with an anti-ProDH antibody and analysing protein spots by MS. The 2D gels showed that ProDH1 forms part of a low-molecular-mass (70-140 kDa) complex in the mitochondrial membrane. To evaluate the contribution of each isoform to proline oxidation, mitochondria were isolated from wild-type (WT) and prodh1, prodh2, prodh1prodh2 and p5cdh mutants. ProDH activity was high for genotypes in which ProDH, most likely ProDH1, was strongly induced by proline. Respiratory measurements indicate that ProDH1 has a role in oxidizing excess proline and transferring electrons to the respiratory chain.


Asunto(s)
Arabidopsis/metabolismo , Transporte de Electrón , Mitocondrias/metabolismo , Prolina Oxidasa/metabolismo , Prolina/metabolismo , Proteoma , Arabidopsis/enzimología , Electroforesis en Gel de Poliacrilamida , Espectrometría de Masas
3.
Plant Cell Physiol ; 53(1): 183-92, 2012 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-22121247

RESUMEN

Proline accumulation is one of the most common responses of plants to environmental constraints. Thellungiella halophila/salsuginea, a model halophyte, accumulates high levels of proline in response to abiotic stress and in the absence of stress. Recently, lipid signaling pathways have been shown to be involved in the regulation of proline metabolism in Arabidopsis thaliana. Here we investigated the relationship between lipid signaling enzymes and the level of proline in T. salsuginea. Inhibition of phospholipase C (PLC) enzymes by the specific inhibitor U73122 demonstrated that proline accumulation is negatively controlled by PLCs in the absence of stress and under moderate salt stress (200 mM NaCl). The use of 1-butanol to divert some of the phospholipase D (PLD)-derived phosphatidic acid by transphosphatidylation revealed that PLDs exert a positive control on proline accumulation under severe stress (400 mM NaCl or 400 mM mannitol) but have no effect on its accumulation in non-stress conditions. This experimental evidence shows that positive and negative lipid regulatory components are involved in the fine regulation of proline metabolism. These signaling pathways in T. salsuginea are regulated in the opposite sense to those previously described in A. thaliana, revealing that common signaling components affect the physiology of closely related glycophyte and salt-tolerant plants differently.


Asunto(s)
Brassicaceae/enzimología , Fosfolipasa D/metabolismo , Prolina/metabolismo , Cloruro de Sodio/farmacología , Estrés Fisiológico/efectos de los fármacos , Fosfolipasas de Tipo C/metabolismo , 1-Butanol/farmacología , Brassicaceae/efectos de los fármacos , Estrenos/farmacología , Espacio Extracelular/efectos de los fármacos , Espacio Extracelular/metabolismo , Metabolismo de los Lípidos/efectos de los fármacos , Manitol/farmacología , Modelos Biológicos , Ósmosis/efectos de los fármacos , Pirrolidinonas/farmacología , Transducción de Señal/efectos de los fármacos
4.
Nat Commun ; 13(1): 3586, 2022 06 23.
Artículo en Inglés | MEDLINE | ID: mdl-35739107

RESUMEN

Impaired activity of the chloride channel CFTR is the cause of cystic fibrosis. 14-3-3 proteins have been shown to stabilize CFTR and increase its biogenesis and activity. Here, we report the identification and mechanism of action of a macrocycle stabilizing the 14-3-3/CFTR complex. This molecule rescues plasma membrane localization and chloride transport of F508del-CFTR and works additively with the CFTR pharmacological chaperone corrector lumacaftor (VX-809) and the triple combination Trikafta®. This macrocycle is a useful tool to study the CFTR/14-3-3 interaction and the potential of molecular glues in cystic fibrosis therapeutics.


Asunto(s)
Regulador de Conductancia de Transmembrana de Fibrosis Quística , Fibrosis Quística , Aminofenoles/metabolismo , Aminopiridinas/metabolismo , Aminopiridinas/farmacología , Membrana Celular/metabolismo , Fibrosis Quística/tratamiento farmacológico , Fibrosis Quística/metabolismo , Regulador de Conductancia de Transmembrana de Fibrosis Quística/genética , Regulador de Conductancia de Transmembrana de Fibrosis Quística/metabolismo , Humanos , Mutación
5.
Plant Physiol ; 152(4): 1851-62, 2010 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-20172963

RESUMEN

Mitochondrial carrier family proteins are diverse in their substrate specificity, organellar location, and gene expression. In Arabidopsis (Arabidopsis thaliana), 58 genes encode these six-transmembrane-domain proteins. We investigated the biological role of the basic amino acid carrier Basic Amino Acid Carrier2 (BAC2) from Arabidopsis that is structurally and functionally similar to ARG11, a yeast ornithine and arginine carrier, and to Arabidopsis BAC1. By studying the expression of BAC2 and the consequences of its mutation in Arabidopsis, we showed that BAC2 is a genuine mitochondrial protein and that Arabidopsis requires expression of the BAC2 gene in order to use arginine. The BAC2 gene is induced by hyperosmotic stress (with either 0.2 m NaCl or 0.4 m mannitol) and dark-induced senescence. The BAC2 promoter contains numerous stress-related cis-regulatory elements, and the transcriptional activity of BAC2:beta-glucuronidase is up-regulated by stress and senescence. Under hyperosmotic stress, bac2 mutants express the P5CS1 proline biosynthetic gene more strongly than the wild type, and this correlates with a greater accumulation of Pro. Our data suggest that BAC2 is a hyperosmotic stress-inducible transporter of basic amino acids that contributes to proline accumulation in response to hyperosmotic stress in Arabidopsis.


Asunto(s)
Sistemas de Transporte de Aminoácidos Básicos/genética , Proteínas de Arabidopsis/genética , Arabidopsis/metabolismo , Mutación , Prolina/metabolismo , Sistemas de Transporte de Aminoácidos Básicos/metabolismo , Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Datos de Secuencia Molecular , Presión Osmótica , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Transcripción Genética
6.
FEBS Lett ; 566(1-3): 115-20, 2004 May 21.
Artículo en Inglés | MEDLINE | ID: mdl-15147879

RESUMEN

Increasing evidence suggests a major role for phosphatidylcholine (PC) in plant stress adaptation. The present work investigated the regulation of choline, PC and interconnected phosphatidylethanolamine biosynthesis in Arabidopsis thaliana L. as a function of cold- and salt- or mannitol-mediated hyperosmotic stresses. While PC synthesis is accelerated in both salt- and cold-treated plants, the choline kinase (CK) and phosphocholine cytidylyltransferase genes are oppositely regulated with respect to these abiotic treatments. Salt stress also stimulates CK activity in vitro. A possible regulatory role of CK in stimulating PC biosynthesis rate in salt-stressed plants is discussed.


Asunto(s)
Arabidopsis/enzimología , Colina Quinasa/metabolismo , Fosfatidilcolinas/biosíntesis , Cloruro de Sodio/farmacología , Arabidopsis/metabolismo , Northern Blotting , Radioisótopos de Carbono , Frío , Citidina Difosfato Colina/metabolismo , Citosol/enzimología , Expresión Génica , Soluciones Hipertónicas/farmacología , Manitol/farmacología , Presión Osmótica , Fosfatidilcolinas/genética , Fosfatidiletanolaminas/biosíntesis , Transcripción Genética/efectos de los fármacos
7.
Front Biosci (Landmark Ed) ; 17(2): 607-20, 2012 01 01.
Artículo en Inglés | MEDLINE | ID: mdl-22201764

RESUMEN

Proline dehydrogenase (ProDH), also called proline oxidase (POX), is a universal enzyme in living organisms. It catalyzes the oxidation of L-proline to delta1-pyrroline-5-carboxylate leading to the release of electrons, which can be transferred to either electron transfer systems or to molecular oxygen. ProDH is not only essential for proline catabolism but also plays key roles in providing energy, shuttling redox potential between cellular compartments and reactive oxygen species production. Structural analysis of prokaryotic ProDHs already gives some insights into the biochemical activity and biological functions of this enzyme, which can be extended to eukaryotic ProDHs based on sequence similarities. Here we report the most recent investigations on the biochemical and regulation of ProDH at transcriptional, post-transcriptional and translational levels. The biological roles of ProDH in cell homeostasis and adaptation through energetic, developmental, adaptive, physiological and pathological processes in eukaryotes are presented and discussed to create a framework for future research direction.


Asunto(s)
Prolina Oxidasa/genética , Prolina Oxidasa/metabolismo , Animales , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Ciclo Celular , Discapacidades del Desarrollo/genética , Discapacidades del Desarrollo/metabolismo , Metabolismo Energético , Homeostasis , Humanos , Proteínas de la Membrana/genética , Proteínas de la Membrana/metabolismo , Enfermedades Metabólicas/genética , Enfermedades Metabólicas/metabolismo , Modelos Biológicos , Filogenia , Procesamiento Proteico-Postraduccional , Especies Reactivas de Oxígeno/metabolismo , Estrés Fisiológico , Transcripción Genética
8.
Methods Mol Biol ; 639: 333-40, 2010.
Artículo en Inglés | MEDLINE | ID: mdl-20387057

RESUMEN

Proline is a key factor in plant adaptation to environmental stresses. The Delta(1)-pyrroline-5-carboxylate synthetase catalyzes the first committed step and the rate-limiting step for proline biosynthesis in both plants and mammals. This enzyme catalyzes the reduction of glutamate to pyrroline-5-carboxylate in two sequential steps including the phosphorylation and the reduction of its precursor. Several methods were established to assay P5CS activity but however none of them are fully reliable. Therefore, we developed a new simple and reliable assay which is based on the quantification of Pi. This assay allowed us to determine the optimal pH, the apparent K(m) and V(m) of P5CS with regard to ATP and glutamate.


Asunto(s)
Arabidopsis/enzimología , Pruebas de Enzimas/métodos , Fosfotransferasas (Aceptor de Grupo Alcohol)/metabolismo , Adenosina Trifosfato/metabolismo , Ácido Glutámico/metabolismo , Concentración de Iones de Hidrógeno , Cinética , Extractos Vegetales/metabolismo , Hojas de la Planta/enzimología , Solubilidad , Especificidad por Sustrato
9.
J Plant Physiol ; 165(6): 588-99, 2008 Apr 18.
Artículo en Inglés | MEDLINE | ID: mdl-17723252

RESUMEN

The eco-physiology of salt tolerance, with an emphasis on K(+) nutrition and proline accumulation, was investigated in the halophyte Thellungiella halophila and in both wild type and eskimo-1 mutant of the glycophyte Arabidopsis thaliana, which differ in their proline accumulation capacity. Plants cultivated in inert sand were challenged for 3 weeks with up to 500mM NaCl. Low salinity significantly decreased A. thaliana growth, whereas growth restriction was significant only at salt concentrations equal to or exceeding 300mM NaCl in T. halophila. Na(+) content generally increased with the amount of salt added in the culture medium in both species, but T. halophila showed an ability to control Na(+) accumulation in shoots. The analysis of the relationship between water and Na(+) contents suggested an apoplastic sodium accumulation in both species; this trait was more pronounced in A. thaliana than in T. halophila. The better NaCl tolerance in the latter was associated with a better K(+) supply, resulting in higher K(+)/Na(+) ratios. It was also noteworthy that, despite highly accumulating proline, the A. thaliana eskimo-1 mutant was the most salt-sensitive species. Taken together, our findings indicate that salt tolerance may be partly linked to the plants' ability to control Na(+) influx and to ensure appropriate K(+) nutrition, but is not linked to proline accumulation.


Asunto(s)
Arabidopsis/metabolismo , Brassicaceae/metabolismo , Potasio/metabolismo , Prolina/metabolismo , Tolerancia a la Sal , Sodio/metabolismo , Arabidopsis/efectos de los fármacos , Arabidopsis/crecimiento & desarrollo , Biomasa , Brassicaceae/efectos de los fármacos , Brassicaceae/crecimiento & desarrollo , Salinidad , Tolerancia a la Sal/efectos de los fármacos , Cloruro de Sodio/farmacología , Estrés Fisiológico/efectos de los fármacos , Agua/metabolismo
10.
Plant Physiol ; 146(2): 554-65, 2008 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-18065553

RESUMEN

Wood cells, unlike most other cells in plants, grow by a unique combination of intrusive and symplastic growth. Fibers grow in diameter by diffuse symplastic growth, but they elongate solely by intrusive apical growth penetrating the pectin-rich middle lamella that cements neighboring cells together. In contrast, vessel elements grow in diameter by a combination of intrusive and symplastic growth. We demonstrate that an abundant pectin methyl esterase (PME; EC 3.1.1.11) from wood-forming tissues of hybrid aspen (Populus tremula x tremuloides) acts as a negative regulator of both symplastic and intrusive growth of developing wood cells. When PttPME1 expression was up- and down-regulated in transgenic aspen trees, the PME activity in wood-forming tissues was correspondingly altered. PME removes methyl ester groups from homogalacturonan (HG) and transgenic trees had modified HG methylesterification patterns, as demonstrated by two-dimensional nuclear magnetic resonance and immunostaining using PAM1 and LM7 antibodies. In situ distributions of PAM1 and LM7 epitopes revealed changes in pectin methylesterification in transgenic trees that were specifically localized in expanding wood cells. The results show that en block deesterification of HG by PttPME1 inhibits both symplastic growth and intrusive growth. PttPME1 is therefore involved in mechanisms determining fiber width and length in the wood of aspen trees.


Asunto(s)
Hidrolasas de Éster Carboxílico/metabolismo , Populus/citología , Populus/enzimología , Madera/citología , Madera/enzimología , Clonación Molecular , Regulación de la Expresión Génica de las Plantas/fisiología , Inmunoquímica , Espectroscopía de Resonancia Magnética , Datos de Secuencia Molecular , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Modificadas Genéticamente , Populus/crecimiento & desarrollo , Isoformas de Proteínas , Madera/crecimiento & desarrollo
11.
Plant Physiol ; 144(1): 503-12, 2007 May.
Artículo en Inglés | MEDLINE | ID: mdl-17369432

RESUMEN

Proline (Pro) accumulation occurs in various plant organisms in response to environmental stresses. To identify the signaling components involved in the regulation of Pro metabolism upon water stress in Arabidopsis (Arabidopsis thaliana), a pharmacological approach was developed. The role of phosphoinositide-specific phospholipases C (PLCs) in Pro accumulation was assessed by the use of the aminosteroid U73122, a commonly employed specific inhibitor of receptor-mediated PLCs. We found that U73122 reduced pyrroline-5-carboxylate synthetase transcript and protein as well as Pro levels in salt-treated seedlings. Inhibition of PLC activity by U73122 was quantified by measuring the decrease of inositol 1,4,5-trisphosphate (InsP(3)) levels. Moreover, the utilization of diacylglycerol kinase and InsP(3)-gated calcium release receptor inhibitors suggested that InsP(3) or its derivatives are essential for Pro accumulation upon salt stress, involving calcium as a second messenger in ionic stress signaling. This observation was further supported by a partial restoration of Pro accumulation in salt- and U73122-treated seedlings after addition of extracellular calcium, or when calcium homeostasis was perturbed by cyclopiazonic acid, a blocker of plant type IIA calcium pumps. Taken together, our data indicate that PLC-based signaling is a committed step in Pro biosynthesis upon salinity but not in the case of mannitol stress. Calcium acts as a molecular switch to trigger downstream signaling events. These results also demonstrated the specific involvement of lipid signaling pathway to discriminate between ionic and nonionic stresses.


Asunto(s)
Arabidopsis/metabolismo , Señalización del Calcio/fisiología , Calcio/metabolismo , Presión Osmótica , Prolina/metabolismo , Fosfolipasas de Tipo C/metabolismo , Arabidopsis/efectos de los fármacos , Arabidopsis/fisiología , Compuestos de Boro/farmacología , Estrenos/farmacología , Indoles/farmacología , Inositol 1,4,5-Trifosfato/metabolismo , Datos de Secuencia Molecular , Pirimidinonas/farmacología , Pirrolidinonas/farmacología , Plantones/efectos de los fármacos , Plantones/metabolismo , Plantones/fisiología , Cloruro de Sodio/farmacología , Tiazoles/farmacología , Agua/metabolismo
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA