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1.
Biochim Biophys Acta ; 1334(2-3): 283-90, 1997 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-9101724

RESUMEN

Human skin chronically exposed to UV light is known to accumulate iron and to have an increased ferritin content as compared to unexposed areas. Iron accumulation is also found in many inflammatory skin diseases. Cultured human fibroblasts loaded with iron by incubation with non-toxic concentrations of the ferric nitrilotriacetate complex have been irradiated with low (up to 15 J/cm2) and moderate (up to 45 J/cm2) UVA doses. At low irradiation doses, lipid peroxidation doubles without affecting the viability of iron-loaded cells. At higher irradiation doses (30 J/cm2) the photocytotoxicity of UVA towards iron-loaded cells increases in a concentration-dependent manner with the iron load. Thus, after exposure to 30 J/cm2 of UVA, the cytotoxicity is about 3-fold greater for cells incubated for 75 min with 100 microM of the ferric complex as compared to those not treated with the ferric complex. Incubation with desferrioxamine, an extremely efficient chelator of ferric ion or vitamin E, a radical scavenger which blocks the lipid peroxidation radical chain, leads to marked inhibition of the sensitizing effects of iron on lipid peroxidation but is less effective for the survival of cells exposed to UVA. A similar concentration-dependent protective effect of desferrioxamine was observed with cultured fibroblasts not treated with the ferric complex. It is suggested that the photoreduction of ferritin and/or other iron-containing proteins plays a significant role in the UVA-induced photocytotoxicity of skin fibroblasts.


Asunto(s)
Compuestos Férricos/farmacología , Ácido Nitrilotriacético/análogos & derivados , Fármacos Sensibilizantes a Radiaciones/farmacología , Piel/efectos de la radiación , Rayos Ultravioleta , Supervivencia Celular , Células Cultivadas , Deferoxamina/farmacología , Relación Dosis-Respuesta en la Radiación , Compuestos Férricos/antagonistas & inhibidores , Fibroblastos/efectos de la radiación , Humanos , Peroxidación de Lípido , Ácido Nitrilotriacético/antagonistas & inhibidores , Ácido Nitrilotriacético/farmacología , Piel/efectos de los fármacos , Sustancias Reactivas al Ácido Tiobarbitúrico/análisis
2.
J Comp Pathol ; 153(4): 231-5, 2015 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-26297109

RESUMEN

A mature male, black and white ruffed lemur (Varecia variegata variegata) died in a zoological garden after a 4-day history of lethargy and non-responsive convulsions. Necropsy and histopathological examinations revealed acute necrotizing and haemorrhagic meningoencephalitis with intralesional amoebas confirmed by immunohistochemistry. Acanthamoeba T4 genotype was identified as the causative agent of the brain lesion, based on amplification and sequencing of 18S ribosomal RNA genes. The presence of free-living amoebas in water and mud from the lemur's environment was investigated by morphological and molecular analyses. The two predominant genera, representing 80% of isolated amoebas, were Naegleria spp. and Acanthamoeba spp. All Acanthamoeba isolates belonged to the T4 genotype. To the author's knowledge, this is the first report of a meningoencephalitis due to Acanthamoeba T4 genotype in Lemuridae with concurrent analysis of pathological tissues and environment.


Asunto(s)
Acanthamoeba/genética , Amebiasis/veterinaria , Lemur/microbiología , Meningoencefalitis/veterinaria , Amebiasis/patología , Animales , Genotipo , Masculino , Meningoencefalitis/microbiología , Meningoencefalitis/patología , Necrosis/microbiología , Necrosis/veterinaria
3.
J Photochem Photobiol B ; 54(1): 43-54, 2000 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-10739142

RESUMEN

Iron and/or ferritin accumulation are known to occur under pathological conditions in many inflammatory skin diseases or in human skin chronically exposed to UV light. Under such conditions, ferritin is believed to play an effective protective role in accommodating and 'deactivating' excess 'free' iron produced by the inflammatory process or the UV illumination. The present study compares the relationship between ferritin over-expression and effects of an oxidative stress induced chemically by tert-butyl hydroperoxide or photochemically by UV-A radiation. As shown by immunoassay, cultured MRC 5 and HS 68 fibroblasts treated for at least one day with transferrin or overnight with non-toxic concentrations of the ferric nitrilotriacetate complex express up to 10 times more ferritin than untreated cells, whereas a five-fold increase is obtained with NCTC 2544 keratinocytes. In all cases a parallel increase in soluble cellular iron is measured by inductive plasma emission spectroscopy. The superoxide dismutase and catalase activities and total glutathione levels are not modified by the iron treatment, whereas a transient increase in the Se-dependent glutathione peroxidase activity of keratinocytes is observed after a short incubation with the iron complex. In keratinocytes and fibroblasts, ferritin over-expression after iron treatment markedly inhibits lipid peroxidation but, paradoxically, not the mortality induced by tert-butyl hydroperoxide. In contrast, this excess ferritin does not protect cells from both the peroxidation and mortality induced by moderate doses (30 J/cm2) of UV-A radiation. As a consequence, protection against oxidative damage by excess ferritin synthesis clearly depends on the nature of the oxidative stress on cell targets and it seems to be of lesser importance in the case of photochemically induced oxidation.


Asunto(s)
Compuestos Férricos/farmacología , Ferritinas/metabolismo , Fibroblastos/metabolismo , Queratinocitos/metabolismo , Ácido Nitrilotriacético/análogos & derivados , Estrés Oxidativo , Rayos Ultravioleta , terc-Butilhidroperóxido/farmacología , Catalasa/metabolismo , Línea Celular , Supervivencia Celular/efectos de los fármacos , Supervivencia Celular/efectos de la radiación , Fibroblastos/efectos de los fármacos , Fibroblastos/efectos de la radiación , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Humanos , Hierro/metabolismo , Queratinocitos/efectos de los fármacos , Queratinocitos/efectos de la radiación , Pulmón , Ácido Nitrilotriacético/farmacología , Superóxido Dismutasa/metabolismo , Sustancias Reactivas al Ácido Tiobarbitúrico/análisis
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