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1.
Mol Reprod Dev ; 85(8-9): 682-695, 2018 08.
Artículo en Inglés | MEDLINE | ID: mdl-30118583

RESUMEN

Members of the solute carrier 26 (SLC26) family have emerged as important players in mediating anions fluxes across the plasma membrane of epithelial cells, in cooperation with the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel. Among them, SLC26A3 acts as a chloride/bicarbonate exchanger, highly expressed in the gastrointestinal, pancreatic and renal tissues. In humans, mutations in the SLC26A3 gene were shown to induce congenital chloride-losing diarrhea (CLD), a rare autosomal recessive disorder characterized by life-long secretory diarrhea. In view of some reports indicating subfertility in some male CLD patients together with SLC26-A3 and -A6 expression in the male genital tract and sperm cells, we analyzed the male reproductive parameters and functions of SLC26A3 deficient mice, which were previously reported to display CLD gastro-intestinal features. We show that in contrast to Slc26a6, deletion of Slc26a3 is associated with severe lesions and abnormal cytoarchitecture of the epididymis, together with sperm quantitative, morphological and functional defects, which altogether compromised male fertility. Overall, our work provides new insight into the pathophysiological mechanisms that may alter the reproductive functions and lead to male subfertility in CLD patients, with a phenotype reminiscent of that induced by CFTR deficiency in the male genital tract.


Asunto(s)
Antiportadores/metabolismo , Epidídimo/metabolismo , Epidídimo/fisiopatología , Fertilización , Infertilidad Masculina/metabolismo , Capacitación Espermática , Transportadores de Sulfato/metabolismo , Animales , Antiportadores/genética , Regulador de Conductancia de Transmembrana de Fibrosis Quística/metabolismo , Diarrea/congénito , Diarrea/etiología , Masculino , Errores Innatos del Metabolismo/etiología , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Mutación , Fenotipo , Recuento de Espermatozoides , Motilidad Espermática , Espermatozoides/patología , Transportadores de Sulfato/genética , Testículo/fisiopatología
2.
Transpl Int ; 23(1): 71-9, 2010 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-19678902

RESUMEN

Adhesion and degranulation promoting adapter protein (ADAP) plays an important role in T cell activation. ADAP deficiency was recently found to prolong heart graft survival in mice. We investigated the role of ADAP in intestinal transplantation and the synergistic effect of ADAP deficiency and Costimulation blockade (CB). T cell proliferation and cytotoxic T lymphocyte (CTL) activity were determined. MHC mismatched intestinal allografts was transplanted heterotopically. Anti-CD40L antibody was applied to the recipient. Upon stimulation with allogenic dendritic cells (DC), ADAP-deficient (ADAP-/-) T cells displayed impaired proliferative responses compared with that of wild-type (WT) T cells. In contrast, the CTL activity in ADAP-/- mice was comparable with that of WT mice. Rejection of intestinal allografts was ameliorated, but not prevented in ADAP-/- mice. Although CB alone was not sufficient to mitigate the rejection, the combination of CB and ADAP deficiency profoundly inhibited rejection. This was accompanied by less infiltration and activation of host lymphocytes in the gut-associated lymphoid tissue of intestinal allografts. ADAP deficiency combined with CB protected the intestinal allografts synergistically. ADAP could be a novel target in the induction phase of the immune responses in organ transplantation.


Asunto(s)
Proteínas Adaptadoras Transductoras de Señales/deficiencia , Rechazo de Injerto/inmunología , Intestinos/trasplante , Animales , Linfocitos T CD4-Positivos/metabolismo , Linfocitos T CD8-positivos/metabolismo , Proliferación Celular , Células Dendríticas/inmunología , Supervivencia de Injerto/inmunología , Intestinos/patología , Selectina L/metabolismo , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Linfocitos T Citotóxicos/inmunología , Linfocitos T Citotóxicos/fisiología
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