Genetic and virulence variability among Erwinia tracheiphila strains recovered from different cucurbit hosts.

The causal agent of cucurbit bacterial wilt, Erwinia tracheiphila, has a wide host range in the family Cucurbitaceae, including economically important crops such as muskmelon (Cucumis melo), cucumber (C. sativus), and squash (Cucurbita spp.). Genetic variability of 69 E. tracheiphila strains was investigated by repetitive-element polymerase chain reaction (rep-PCR) using BOXA1R and ERIC1-2 primers. Fingerprint profiles revealed significant variability associated with crop host; strains isolated from Cucumis spp. were clearly distinguishable from Cucurbita spp.-isolated strains regardless of geographic origin. Twelve E. tracheiphila strains isolated from muskmelon, cucumber, or summer squash were inoculated onto muskmelon and summer squash seedlings, followed by incubation in a growth chamber. Wilt symptoms were assessed over 3 weeks, strains were reisolated, and rep-PCR profiles were compared with the inoculated strains. Wilting occurred significantly faster when seedlings were inoculated with strains that originated from the same crop host genus (P<0.001). In the first run of the experiment, cucumber and muskmelon strains caused wilting on muskmelon seedlings at a median of 7.8 and 5.6 days after inoculation (dai), respectively. Summer squash seedlings wilted 18.0, 15.7, and 5.7 dai when inoculated with muskmelon-, cucumber-, and squash-origin strains, respectively. In a second run of the experiment, cucumber and muskmelon strains caused wilting on muskmelon at 7.0 and 6.9 dai, respectively, whereas summer squash seedlings wilted at 23.6, 29.0 and 9.0 dai when inoculated with muskmelon-, cucumber-, and squash-origin strains, respectively. Our results provide the first evidence of genetic diversity within E. tracheiphila and suggest that strain specificity is associated with plant host. This advance is a first step toward understanding the genetic and population structure of E. tracheiphila.

Epiphytic Survival of Erwinia tracheiphila on Muskmelon (Cucumis melo L.).

Erwinia tracheiphila, the causal agent of bacterial wilt of cucurbits, is transmitted by striped (Acalymma vittatum) and spotted (Diabrotica undecimpunctata howardi) cucumber beetles. Transmission occurs when infested frass with E. tracheiphila is deposited on plant surfaces with fresh feeding wounds. However, it is unclear whether the pathogen can survive as an epiphyte on leaves. Experiments were conducted in controlled environments to monitor E. tracheiphila survival on muskmelon (Cucumis melo) leaves under various temperature and moisture conditions. In the first experiment, muskmelon seedlings that had been spray inoculated with a rifampicin-resistant strain of E. tracheiphila were incubated at 10, 15, 20, 25, 30, or 35°C (±2°C) at ≥95% relative humidity, and E. tracheiphila populations were monitored for 72 h. In the second experiment, E. tracheiphila was monitored during alternating 12-h wet and dry periods, or continuous wet or dry conditions for 48 h at 20°C. Survival of E. tracheiphila on wet muskmelon leaves depended on temperature (P < 0.01), with the greatest survival at 10 and 15°C and least at 30 and 35°C. Leaf wetness also impacted survival; an initial 12-h dry period resulted in a 1,000- to 10,000-fold reduction in population size, followed by stabilization of the surviving population. These results demonstrate that E. tracheiphila can survive on muskmelon leaves under a wide range of environmental conditions, suggesting that epiphytic populations might serve as a reservoir of inoculum for infections.

Feasibility of Delaying Removal of Row Covers to Suppress Bacterial Wilt of Muskmelon (Cucumis melo).

Bacterial wilt, caused by Erwinia tracheiphila, is a major disease of cucurbit crops in the United States. Management of the disease relies on controlling two vector species, striped (Acalymma vittatum) and spotted (Diabrotica undecimpunctata) cucumber beetles. Six field trials were conducted at Iowa State University research farms during 2007, 2008, and 2009 to assess the efficacy of delayed removal of spunbond polypropylene row covers to control bacterial wilt on muskmelon (Cucumis melo). Treatments were (i) row cover removed at anthesis (conventional timing of removal), (ii) covers removed 10 days after row cover ends were opened at anthesis, (iii) covers removed 10 days after bumble bee hives were inserted under row covers at anthesis, and (iv) a noncovered control. In two field trials during 2007 and 2008, the delayed-removal row-cover treatments significantly suppressed bacterial wilt throughout the growing season and enhanced yield compared with the noncovered and removal-at-anthesis controls. In Gilbert in 2008, however, bacterial wilt suppression was equivalent among all three row-cover treatments. No bacterial wilt was observed during three trials in 2009, and there was minimal difference in marketable yield among treatments. Net returns were compared using partial budget and sensitivity analyses. Melon prices and occurrence of bacterial wilt had a strong impact on net returns. Using row covers increased production costs by 45%. In site years in which bacterial wilt occurred, delaying removal of row covers resulted in the highest returns. When bacterial wilt was absent, however, the delayed-removal row-cover treatments had the lowest returns. Results of the sensitivity analysis indicated that delaying removal of row covers for 10 days could be a cost-effective component of an integrated bacterial wilt suppression strategy for muskmelon where bacterial wilt occurs ≥50% of production seasons.