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1.
Am J Hum Genet ; 101(5): 725-736, 2017 Nov 02.
Artículo en Inglés | MEDLINE | ID: mdl-29100086

RESUMEN

The transatlantic slave trade was the largest forced migration in world history. However, the origins of the enslaved Africans and their admixture dynamics remain unclear. To investigate the demographic history of African-descendant Marron populations, we generated genome-wide data (4.3 million markers) from 107 individuals from three African-descendant populations in South America, as well as 124 individuals from six west African populations. Throughout the Americas, thousands of enslaved Africans managed to escape captivity and establish lasting communities, such as the Noir Marron. We find that this population has the highest proportion of African ancestry (∼98%) of any African-descendant population analyzed to date, presumably because of centuries of genetic isolation. By contrast, African-descendant populations in Brazil and Colombia harbor substantially more European and Native American ancestry as a result of their complex admixture histories. Using ancestry tract-length analysis, we detect different dates for the European admixture events in the African-Colombian (1749 CE; confidence interval [CI]: 1737-1764) and African-Brazilian (1796 CE; CI: 1789-1804) populations in our dataset, consistent with the historically attested earlier influx of Africans into Colombia. Furthermore, we find evidence for sex-specific admixture patterns, resulting from predominantly European paternal gene flow. Finally, we detect strong genetic links between the African-descendant populations and specific source populations in Africa on the basis of haplotype sharing patterns. Although the Noir Marron and African-Colombians show stronger affinities with African populations from the Bight of Benin and the Gold Coast, the African-Brazilian population from Rio de Janeiro has greater genetic affinity with Bantu-speaking populations from the Bight of Biafra and west central Africa.


Asunto(s)
Población Negra/genética , África , Brasil , Femenino , Guyana Francesa , Flujo Génico/genética , Genética de Población , Estudio de Asociación del Genoma Completo/métodos , Haplotipos , Hispánicos o Latinos/genética , Humanos , Masculino , Polimorfismo de Nucleótido Simple/genética , Suriname , Población Blanca/genética
2.
Nature ; 488(7411): 370-4, 2012 Aug 16.
Artículo en Inglés | MEDLINE | ID: mdl-22801491

RESUMEN

The peopling of the Americas has been the subject of extensive genetic, archaeological and linguistic research; however, central questions remain unresolved. One contentious issue is whether the settlement occurred by means of a single migration or multiple streams of migration from Siberia. The pattern of dispersals within the Americas is also poorly understood. To address these questions at a higher resolution than was previously possible, we assembled data from 52 Native American and 17 Siberian groups genotyped at 364,470 single nucleotide polymorphisms. Here we show that Native Americans descend from at least three streams of Asian gene flow. Most descend entirely from a single ancestral population that we call 'First American'. However, speakers of Eskimo-Aleut languages from the Arctic inherit almost half their ancestry from a second stream of Asian gene flow, and the Na-Dene-speaking Chipewyan from Canada inherit roughly one-tenth of their ancestry from a third stream. We show that the initial peopling followed a southward expansion facilitated by the coast, with sequential population splits and little gene flow after divergence, especially in South America. A major exception is in Chibchan speakers on both sides of the Panama isthmus, who have ancestry from both North and South America.


Asunto(s)
Emigración e Inmigración/historia , Indígenas Norteamericanos/genética , Indígenas Norteamericanos/historia , Filogenia , Américas , Asia , Análisis por Conglomerados , Emigración e Inmigración/estadística & datos numéricos , Flujo Génico , Genética de Población , Historia Antigua , Humanos , Modelos Genéticos , Polimorfismo de Nucleótido Simple/genética , Siberia
3.
Ann Hum Biol ; 42(2): 195-8, 2015 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-25026367

RESUMEN

BACKGROUND: The apolipoprotein E (APOE) gene plays a pivotal role in cholesterol metabolism. Since the discovery of the APOE*2 and APOE*4 as the major susceptibility alleles for several diseases including dyslipidemia, atherosclerosis, coronary heart disease, late-onset and early Alzheimer's disease, the APOE genotype might be considered as a potential predictive factor for both epidemiological research and diagnosis. AIM: The aim of this study is to report on the polymorphism of the APOE gene in the "Paisa" population from northwest Colombia (Antioquia) to obtain a population baseline of the existing variation in this locus. METHOD: One thousand and one healthy voluntaries were genotyped for the APOE polymorphism using polymerase chain reaction-restriction fragment length polymorphism technique. RESULTS: The APOE*3/*3 genotype presented the highest frequency (66.33%) and the APOE*4/*4 had the lowest frequency (1.89%). Genotype frequencies comply with Hardy-Weinberg expectations. Allele frequencies obtained for APOE*2, APOE*3 and APOE*4 were 0.075 ± 0.005 (95% CI = 0.063-0.086), 0.814 ± 0.009 (0.797-0.831) and 0.111 ± 0.007 (0.098-0.125), respectively. CONCLUSION: Although globally the high-to-low APOE frequency follows the E*3 > E*4 > E*2 trend, the present APOE frequency data is in disagreement with some reports from South-American countries.


Asunto(s)
Apolipoproteínas E/genética , Frecuencia de los Genes , Polimorfismo Genético , Colombia , Humanos , Reacción en Cadena de la Polimerasa
4.
PLoS One ; 18(5): e0285264, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37141293

RESUMEN

Dental size variation in modern humans has been assessed from regional to worldwide scales, especially under microevolutionary and forensic contexts. Despite this, populations of mixed continental ancestry such as contemporary Latin Americans remain unexplored. In the present study we investigated a large Latin American sample from Colombia (N = 804) and obtained buccolingual and mesiodistal diameters and three indices for maxillary and mandibular teeth (except third molars). We evaluated the correlation between 28 dental measurements (and three indices) with age, sex and genomic ancestry (estimated using genome-wide SNP data). In addition, we explored correlation patterns between dental measurements and the biological affinities, based on these measurements, between two Latin American samples (Colombians and Mexicans) and three putative parental populations: Central and South Native Americans, western Europeans and western Africans through PCA and DFA. Our results indicate that Latin Americans have high dental size diversity, overlapping the variation exhibited by the parental populations. Several dental dimensions and indices have significant correlations with sex and age. Western Europeans presented closer biological affinities with Colombians, and the European genomic ancestry exhibited the highest correlations with tooth size. Correlations between tooth measurements reveal distinct dental modules, as well as a higher integration of postcanine dentition. The effects on dental size of age, sex and genomic ancestry is of relevance for forensic, biohistorical and microevolutionary studies in Latin Americans.


Asunto(s)
Grupos Raciales , Diente , Humanos , Genómica , Hispánicos o Latinos , Grupos Raciales/genética , Diente/anatomía & histología
5.
Commun Biol ; 6(1): 958, 2023 10 10.
Artículo en Inglés | MEDLINE | ID: mdl-37816865

RESUMEN

The Nav1.7 voltage-gated sodium channel plays a key role in nociception. Three functional variants in the SCN9A gene (encoding M932L, V991L, and D1908G in Nav1.7), have recently been identified as stemming from Neanderthal introgression and to associate with pain symptomatology in UK BioBank data. In 1000 genomes data, these variants are absent in Europeans but common in Latin Americans. Analysing high-density genotype data from 7594 Latin Americans, we characterized Neanderthal introgression in SCN9A. We find that tracts of introgression occur on a Native American genomic background, have an average length of ~123 kb and overlap the M932L, V991L, and D1908G coding positions. Furthermore, we measured experimentally six pain thresholds in 1623 healthy Colombians. We found that Neanderthal ancestry in SCN9A is significantly associated with a lower mechanical pain threshold after sensitization with mustard oil and evidence of additivity of effects across Nav1.7 variants. Our findings support the reported association of Neanderthal Nav1.7 variants with clinical pain, define a specific sensory modality affected by archaic introgression in SCN9A and are consistent with independent effects of the Neanderthal variants on Nav1.7 function.


Asunto(s)
Hombre de Neandertal , Umbral del Dolor , Humanos , Animales , Hombre de Neandertal/genética , Dolor/genética , Canal de Sodio Activado por Voltaje NAV1.7/genética , Nocicepción
6.
Commun Biol ; 6(1): 481, 2023 05 08.
Artículo en Inglés | MEDLINE | ID: mdl-37156940

RESUMEN

We report a genome-wide association study of facial features in >6000 Latin Americans based on automatic landmarking of 2D portraits and testing for association with inter-landmark distances. We detected significant associations (P-value <5 × 10-8) at 42 genome regions, nine of which have been previously reported. In follow-up analyses, 26 of the 33 novel regions replicate in East Asians, Europeans, or Africans, and one mouse homologous region influences craniofacial morphology in mice. The novel region in 1q32.3 shows introgression from Neanderthals and we find that the introgressed tract increases nasal height (consistent with the differentiation between Neanderthals and modern humans). Novel regions include candidate genes and genome regulatory elements previously implicated in craniofacial development, and show preferential transcription in cranial neural crest cells. The automated approach used here should simplify the collection of large study samples from across the world, facilitating a cosmopolitan characterization of the genetics of facial features.


Asunto(s)
Hombre de Neandertal , Humanos , Animales , Ratones , Hombre de Neandertal/genética , Estudio de Asociación del Genoma Completo , Nariz , Diferenciación Celular
7.
PLoS One ; 17(7): e0270995, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35802653

RESUMEN

Multilocus Sequence Typing has become a useful tool for the study of the genetic diversity and population structure of different organisms. In this study, a MLST approach with seven loci (CP47, MS5, MS9, MSC6-7, TP14, and gp60) was used to analyze the genetic diversity of Cryptosporidium hominis and Cryptosporidium parvum isolated from 28 Colombian patients. Five Cryptosporidium species were identified: C. hominis, C. parvum, Cryptosporidium felis, Cryptosporidium meleagridis, and Cryptosporidium suis. Unilocus gp60 analysis identified four allelic families for C. hominis (Ia, Ib, Id, and Ie) and two for C. parvum (IIa and IIc). There was polymorphic behavior of all markers evaluated for both C. hominis and C. parvum, particularly with the CP47, MS5, and gp60 markers. Phylogenetic analysis with consensus sequences (CS) of the markers showed a taxonomic agreement with the results obtained with the 18S rRNA and gp60 gene. Additionally, two monophyletic clades that clustered the species C. hominis and C. parvum were detected, with a higher number of subclades within the monophyletic groups compared to those with the gp60 gene. Thirteen MLG were identified for C. hominis and eight for C. parvum. Haplotypic and nucleotide diversity were detected, but only the latter was affected by the gp60 exclusion from the CS analysis. The gene fixation index showed an evolutionary closeness between the C. hominis samples and a less evolutionary closeness and greater sequence divergence in the C. parvum samples. Data obtained in this work support the implementation of MLST analysis in the study of the genetic diversity of Cryptosporidium, considering the more detailed information that it provides, which may explain some genetic events that with an unilocus approach could not be established. This is the first multilocus analysis of the intra-specific variability of Cryptosporidium from humans in South America.


Asunto(s)
Criptosporidiosis , Cryptosporidium parvum , Cryptosporidium , Colombia , Criptosporidiosis/epidemiología , Cryptosporidium parvum/genética , ADN Protozoario/genética , Variación Genética , Genotipo , Humanos , Tipificación de Secuencias Multilocus , Filogenia
8.
PLoS Genet ; 4(3): e1000037, 2008 Mar 21.
Artículo en Inglés | MEDLINE | ID: mdl-18369456

RESUMEN

The large and diverse population of Latin America is potentially a powerful resource for elucidating the genetic basis of complex traits through admixture mapping. However, no genome-wide characterization of admixture across Latin America has yet been attempted. Here, we report an analysis of admixture in thirteen Mestizo populations (i.e. in regions of mainly European and Native settlement) from seven countries in Latin America based on data for 678 autosomal and 29 X-chromosome microsatellites. We found extensive variation in Native American and European ancestry (and generally low levels of African ancestry) among populations and individuals, and evidence that admixture across Latin America has often involved predominantly European men and both Native and African women. An admixture analysis allowing for Native American population subdivision revealed a differentiation of the Native American ancestry amongst Mestizos. This observation is consistent with the genetic structure of pre-Columbian populations and with admixture having involved Natives from the area where the Mestizo examined are located. Our findings agree with available information on the demographic history of Latin America and have a number of implications for the design of association studies in population from the region.


Asunto(s)
Indio Americano o Nativo de Alaska/genética , Población Blanca/genética , Población Negra/genética , Cromosomas Humanos X/genética , Femenino , Variación Genética , Genética de Población , Genoma Humano , Heterocigoto , Humanos , América Latina , Masculino , Repeticiones de Microsatélite
9.
Ann Hum Genet ; 74(6): 525-38, 2010 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-20887376

RESUMEN

We report an integrated analysis of nuclear (autosomal, X- and Y-chromosome) short tandem repeat (STR) data and mtDNA D-loop sequences obtained in the same set of 22 Native populations from across the Americas. A north to south gradient of decreasing population diversity was observed, in agreement with a settlement of the Americas from the extreme northwest of the continent. This correlation is stronger with "least cost distances," which consider the coasts as facilitators of migration. Continent-wide estimates of population structure are highest for the Y-chromosome and lowest for the autosomes, consistent with the effective size of the different marker systems examined. Population differentiation is highest in East South America and lowest in Meso America and the Andean region. Regional analyses suggest a deviation from mutation-drift equilibrium consistent with population expansion in Meso America and the Andes and population contraction in Northwest and East South America. These data hint at an early divergence of Andean and non-Andean South Americans and at a contrasting demographic history for populations from these regions.


Asunto(s)
ADN Mitocondrial/genética , Emigración e Inmigración , Indígenas Norteamericanos/genética , Indígenas Sudamericanos/genética , Cromosomas Humanos X/genética , Cromosomas Humanos Y/genética , Femenino , Humanos , Masculino , Repeticiones de Microsatélite , Análisis de Secuencia de ADN/métodos
10.
Am J Phys Anthropol ; 143(1): 13-20, 2010 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-20734436

RESUMEN

Colombia is a country with great geographic heterogeneity and marked regional differences in pre-Columbian native population density and in the extent of past African and European immigration. As a result, Colombia has one of the most diverse populations in Latin America. Here we evaluated ancestry in over 1,700 individuals from 24 Colombian populations using biparental (autosomal and X-Chromosome), maternal (mtDNA), and paternal (Y-chromosome) markers. Autosomal ancestry varies markedly both within and between regions, confirming the great genetic diversity of the Colombian population. The X-chromosome, mtDNA, and Y-chromosome data indicate that there is a pattern across regions indicative of admixture involving predominantly Native American women and European and African men.


Asunto(s)
Marcadores Genéticos/genética , Genética de Población/métodos , Grupos Raciales/genética , Cromosomas Humanos X/genética , Cromosomas Humanos Y/genética , Colombia , ADN Mitocondrial/genética , Femenino , Geografía , Haplotipos/genética , Humanos , Masculino , Factores Sexuales
11.
PLoS Genet ; 3(11): e185, 2007 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-18039031

RESUMEN

We examined genetic diversity and population structure in the American landmass using 678 autosomal microsatellite markers genotyped in 422 individuals representing 24 Native American populations sampled from North, Central, and South America. These data were analyzed jointly with similar data available in 54 other indigenous populations worldwide, including an additional five Native American groups. The Native American populations have lower genetic diversity and greater differentiation than populations from other continental regions. We observe gradients both of decreasing genetic diversity as a function of geographic distance from the Bering Strait and of decreasing genetic similarity to Siberians--signals of the southward dispersal of human populations from the northwestern tip of the Americas. We also observe evidence of: (1) a higher level of diversity and lower level of population structure in western South America compared to eastern South America, (2) a relative lack of differentiation between Mesoamerican and Andean populations, (3) a scenario in which coastal routes were easier for migrating peoples to traverse in comparison with inland routes, and (4) a partial agreement on a local scale between genetic similarity and the linguistic classification of populations. These findings offer new insights into the process of population dispersal and differentiation during the peopling of the Americas.


Asunto(s)
Variación Genética/genética , Indígenas Norteamericanos/genética , Dinámica Poblacional , Alelos , Cromosomas Humanos/genética , Bases de Datos Genéticas , Emigración e Inmigración , Frecuencia de los Genes , Geografía , Heterocigoto , Humanos , Lenguaje , Lingüística , Filogenia
12.
Gut Microbes ; 11(2): 191-204, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31311405

RESUMEN

Cardiometabolic affections greatly contribute to the global burden of disease. The susceptibility to obesity, cardiovascular disease, and type-2 diabetes, conditions that add to the cardiometabolic syndrome (CMS), was associated with the ancestral genetic composition and gut microbiota. Studies explicitly testing associations between genetic ancestry and gut microbes are growing. We here examined whether the host genetic ancestry was associated with gut microbiota composition, and distinguished the effects of genetic ancestry and non-genetic factors on human cardiometabolic health. We performed a cross-sectional study with 441 community-dwelling Colombian mestizos from five cities spanning the Andes, Pacific, and Caribbean coasts. We characterized the host genetic ancestry by genotyping 40 ancestry informative markers; characterized gut microbiota through 16S rRNA gene sequencing; assessed diet intake, physical activity, cigarette, and medicament consumption; and measured cardiometabolic outcomes that allowed calculating a CMS risk scale. On average, each individual of our cohort was 67 ± 6% European, 21 ± 5% Native American and 12 ± 5% African. Multivariable-adjusted generalized linear models showed that individuals with higher Native American and African ancestries had increased fasting insulin, body mass index and CMS risk, as assessed by the CMS risk scale. Furthermore, we identified 21 OTUs associated to the host genetic ancestry and 20 to cardiometabolic health. While we highlight novel associations between genetic ancestry and gut microbiota, we found that the effect of intestinal microbes was more likely to explain the variance in CMS risk scale than the contributions of European, Native American and African genetic backgrounds.


Asunto(s)
Enfermedades Cardiovasculares/genética , Microbioma Gastrointestinal , Predisposición Genética a la Enfermedad/genética , Factores de Riesgo , Adulto , Negro o Afroamericano/genética , Bacterias/clasificación , Bacterias/genética , Bacterias/aislamiento & purificación , Enfermedades Cardiovasculares/metabolismo , Enfermedades Cardiovasculares/microbiología , Estudios de Cohortes , Estudios Transversales , Dieta , Femenino , Microbioma Gastrointestinal/genética , Microbioma Gastrointestinal/fisiología , Humanos , Indígenas Sudamericanos/genética , Estilo de Vida , Masculino , Metagenómica , Persona de Mediana Edad , ARN Ribosómico 16S , Población Blanca/genética , Adulto Joven
13.
Hum Genet ; 125(5-6): 565-79, 2009 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-19290544

RESUMEN

African descended populations exhibit an increased prevalence of asthma and allergies compared to Europeans. One approach to distinguish between environmental and genetic explanations for this difference is to study relationships of asthma risk to individual admixture. We aimed to determine the admixture proportions of a case-control sample from the Caribbean Coast of Colombia currently participating in genetic studies for asthma, and to test for population stratification and association between African ancestry and asthma and total serum IgE levels (tIgE). We genotyped 368 asthmatics and 365 non-asthmatics for 52 autosomal ancestry informative markers, six mtDNA haplogroups and nine haplogroups and five microsatellites in Y chromosome. Autosomal admixture proportions, population stratification, and associations between ancestry and the phenotypes were estimated by ADMIXMAP. The average admixture proportions among asthmatics were 42.8% European, 39.9% African and 17.2% Native American and among non-asthmatics they were 44.2% (P = 0.068), 37.6% (P = 0.007) and 18.1% (P = 0.050), respectively. In the total sample, the paternal contributions were 71% European, 25% African and 4.0% Native American and the maternal lineages were 56.8% Native American, and 20.2% African; 22.9% of the individuals carried other non-Native American mtDNA haplogroups. African ancestry was significantly associated with asthma (OR: 2.97; 95% CI: 1.08-8.08), high tIgE (OR: 1.9; 95% CI: 1.17-3.12) and socioeconomic status (OR = 0.64; 95% CI: 0.47-0.87). Significant population stratification was observed in this sample. Our findings indicate that genetic factors can explain the association between asthma and African ancestry and suggest that this sample is a useful resource for performing admixture mapping for asthma.


Asunto(s)
Asma/genética , Población Negra/genética , Genética de Población , Inmunoglobulina E/sangre , Adolescente , Adulto , Asma/epidemiología , Estudios de Casos y Controles , Cromosomas Humanos Y/genética , Colombia/epidemiología , ADN Mitocondrial/genética , Femenino , Frecuencia de los Genes , Marcadores Genéticos , Predisposición Genética a la Enfermedad , Genotipo , Humanos , Inmunoglobulina E/genética , Indígenas Norteamericanos/genética , Masculino , Repeticiones de Microsatélite , Persona de Mediana Edad , Factores de Riesgo , Análisis de Secuencia de ADN , Clase Social , Población Blanca/genética , Adulto Joven
14.
Biomedica ; 39(1): 88-101, 2019 03 31.
Artículo en Inglés, Español | MEDLINE | ID: mdl-31021550

RESUMEN

INTRODUCTION: Host genetics is recognized as an influential factor for the development of dengue disease. OBJECTIVE: This study evaluated the association of dengue with the polymorphisms rs8192284 for gene IL6R, rs3775290 for TLR3, and rs7248637 for DC-SIGN. MATERIALS AND METHODS: Of the 292 surveyed subjects, 191 were confirmed for dengue fever and the remaining 101 were included as controls. The genotypes were resolved using polymerase chain reaction and restriction fragment length polymorphism (PCRRFLP). In an attempt to determine the risk (Odds Ratio) of suffering dengue fever, data were analyzed using chi-square for alleles and logistic regression for both genotypes and allelic combinations. Confidence intervals were set to 95% for all tests regardless of the adjustment by either self-identification or ancestry. RESULTS: For Afro-Colombians, the allele rs8192284 C offered protection against dengue [OR=0.425,(0.204-0.887), p=0.020]. The alleles rs7248637 A and rs3775290 A posed, respectively, an increased risk of dengue for Afro-Colombians [OR=2.389, (1.170-4.879), p=0.015] and Mestizos [OR=2.329, (1.283-4.226), p=0.005]. The reproducibility for rs8192284 C/C [OR=2.45, (1.05-5.76), p=0.013] remained after adjustment by Amerindian ancestry [OR=2.52, (1.04-6.09), p=0.013]. The reproducibility for rs3775290 A/A [OR=2.48, (1.09-5.65), p=0.033] remained after adjustment by European [OR=2.34, (1.02-5.35), p=0.048], Amerindian [OR=2.49, (1.09-5.66), p=0.035], and African ancestry [OR=2.37, (1.04-5.41), p=0.046]. Finally, the association of dengue fever with the allelic combination CAG [OR=2.07, (1.06-4.05), p=0.033] remained after adjustment by Amerindian ancestry [OR=2.16, (1.09-4.28), p=0.028]. CONCLUSIONS: Polymorphisms rs8192284 for IL6R, rs3775290 for TLR3, and rs7248637 for DC-SIGN were associated with the susceptibility to suffer dengue fever in the sampled Colombian population.


Introducción. La genética del huésped se reconoce como un factor que influye en el desarrollo del dengue. Objetivo. Este estudio evaluó la asociación del dengue con los polimorfismos rs8192284 del gen IL6R, rs3775290 del TLR3 y rs7248637 del DC-SIGN. Materiales y métodos. De los 292 sujetos encuestados, en 191 se confirmó la presencia de fiebre por dengue y los restantes 101 se incluyeron como controles. Los genotipos se resolvieron mediante reacción en cadena de la polimerasa y polimorfismos en la longitud de los fragmentos de restricción (PCR-RFLP). En un intento por determinar el riesgo de sufrir dengue, los datos se analizaron mediante la prueba de ji al cuadrado para los alelos y la regresión logística para los genotipos y las combinaciones alélicas. Los intervalos de confianza se calcularon a 95 % para todas las pruebas independientemente ajustadas por autoidentificación o componente genético ancestral. Resultados. En los afrocolombianos, el alelo C rs8192284 ofreció protección contra el dengue (OR=0,425; 0,204-0,887, p=0,020). Los alelos A rs7248637 y A rs3775290 plantearon un mayor riesgo de dengue para los afrocolombianos (OR=2,389; 1,170-4,879; p=0,015) y los mestizos (OR=2,329; 1,283-4,226: p=0,005), respectivamente. La reproducibilidad para rs8192284 C/C (OR=2,45; 1,05-5,76; p=0,013) permaneció después del ajuste por el componente genético ancestral amerindio (OR=2,52; 1,04-6,09; p=0,013). La reproducibilidad del rs3775290 A/A (OR=2,48; 1,09-5,65; p=0,033) permaneció después del ajuste por el componente europeo (OR=2,34; 1,02-5,35; p=0,048), el amerindio (OR=2,49; 1,09- 5,66; p=0,035), y el africano (OR=2,37; 1,04-5,41; p=0,046). Por último, la asociación del dengue con la combinación alélica CAG (OR=2,07; 1,06-4,05; p=0,033) permaneció después del ajuste por el componente genético amerindio (OR=2,16; 1,09-4,28; p=0,028). Conclusión. Los polimorfismos rs8192284 en IL6R, rs3775290 en TLR3 y rs7248637 en DC-SIGN, se asociaron con la propensión a sufrir dengue en una muestra de población colombiana.


Asunto(s)
Moléculas de Adhesión Celular/genética , Dengue/genética , Lectinas Tipo C/genética , Polimorfismo de Longitud del Fragmento de Restricción , Receptores de Superficie Celular/genética , Receptores de Interleucina-6/genética , Receptor Toll-Like 3/genética , Adulto , Colombia , Femenino , Predisposición Genética a la Enfermedad , Variación Genética , Humanos , Masculino
15.
Biomedica ; 27 Suppl 1: 50-63, 2007 Jan.
Artículo en Español | MEDLINE | ID: mdl-18154245

RESUMEN

INTRODUCTION: Genetic studies of Trypanosoma cruzi have tried to establish relations between genetic variants and their biological characteristics, such as clinical manifestations, host or geographic origin. However, much controversy exists on the associations between the commonly used DNA markers with group, clinical characteristics and disease epidemiology. OBJECTIVE: In this study determined the variability of the genes that code for the proteins trypanothione reductase and cruzipain, both involved in the infection and survival of the parasite in the mammalian host, was studied and the association between genetic polymorphism and biological and geographic sources in Colombian T. cruzi strains was examined. MATERIALS AND METHODS: The genotypes for each of six SNPs (single nucleotide polymorphisms) for trypanothione reductase and eight SNPs for cruzipain genes were identified by polymerase chain reaction-restriction fragment length polymorphism in 36 T. cruzi Colombian stocks from several regions and biological origins. RESULTS: Three genotypes were identified for trypanothione reductase with Acy I and Hae III enzymes and six genotypes for cruzipain with the Rsa I, Ban I and Bsu 361 enzymes. CONCLUSIONS: For trypanothione reductase, an association was not established with biological or geographical origin; however, alleles at positions 102 and 210 allowed discrimination with groups I and II. For cruzipain, specific genotypes were associated with group, biological and geographic origin. The usefulness of molecular markers on these genes was demonstrated for the determination and differentiation of genetic varieties in T. cruzi.


Asunto(s)
Cisteína Endopeptidasas/genética , NADH NADPH Oxidorreductasas/genética , Polimorfismo de Nucleótido Simple , Trypanosoma cruzi/enzimología , Animales , Antígenos de Protozoos/genética , Biomarcadores/metabolismo , Enfermedad de Chagas/epidemiología , Enfermedad de Chagas/fisiopatología , Colombia/epidemiología , Genotipo , Humanos , Datos de Secuencia Molecular , Polimorfismo de Longitud del Fragmento de Restricción , Proteínas Protozoarias , Trypanosoma cruzi/genética , Trypanosoma cruzi/patogenicidad
16.
Biomedica ; 37(4): 486-497, 2017 Dec 01.
Artículo en Español | MEDLINE | ID: mdl-29373769

RESUMEN

INTRODUCTION: The genetic makeup of the host contributes to the clinical profile of dengue. This could be due to the effect of variants in the genes encoding pro-inflammatory cytokines. OBJECTIVE: To evaluate the association between the variants of three polymorphisms in TNFA, IL6 and IFNG candidate genes with dengue severity in a sample of Colombian population. MATERIALS AND METHODS: We evaluated the rs1800750, rs2069843, and rs2069705 polymorphisms in TNFA, IL6 and IFNG candidate genes, respectively, in 226 patients with dengue infection. The genotypes were typed using both polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). To determine the risk of different dengue phenotypes, we compared allele frequencies with chi-square and genotypes and haplotypes using logistic regression. Finally, these analyzes were adjusted with data from self-identification or the ancestral genetic component. RESULTS: The A allele in the rs2069843 polymorphism, adjusted by self-identification, was associated with dengue hemorrhagic fever cases in Afro-Colombians. In the entire sample, this polymorphism, adjusted by the ancestral genetic component, was reproducible. In addition, there were significant associations between GGT and GAC allelic combinations of rs1800750, rs2069843, and rs2069705 in dengue hemorrhagic fever patients, with and without adjustment by ancestral genetic component. Additionally, the AGC allelic combination produced 58.03 pg/ml of interleukin-6 more than the GGC combination, regardless of European, Amerindian and African genetic components. CONCLUSIONS: The variants of GGT and GAC polymorphisms of rs1800750, rs2069843, and rs2069705 in the TNFA, IL6 and IFNG genes, respectively, were correlated with the susceptibility to dengue severity in a sample of Colombian population.


Asunto(s)
Dengue/genética , Interferón gamma/genética , Interleucina-6/genética , Polimorfismo de Nucleótido Simple , Factor de Necrosis Tumoral alfa/genética , Adolescente , Adulto , Alelos , Niño , Colombia/epidemiología , Estudios Transversales , ADN Viral/genética , Dengue/epidemiología , Virus del Dengue/clasificación , Virus del Dengue/genética , Etnicidad/genética , Femenino , Frecuencia de los Genes , Estudios de Asociación Genética , Predisposición Genética a la Enfermedad , Genotipo , Humanos , Masculino , Persona de Mediana Edad , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de Restricción , Estudios Prospectivos , Riesgo , Adulto Joven
17.
Biomedica ; 26(4): 538-45, 2006 Dec.
Artículo en Español | MEDLINE | ID: mdl-17315480

RESUMEN

INTRODUCTION: Surname frequency (isonymy) is used as a marker of paternal lineage and is used to characterize human population structure. Principles of isonymy were used to determine the genetic structure, migration rates, ancestry relations and origins of populations. This analysis was applied to two historically related local populations which currently are considered to be genetically isolated. OBJECTIVE: The genetic relationships and influence zones of the Aranzazu and Marinilla populations were assessed by means of surname frequency analysis. MATERIALS AND METHODS: Data originated from database with the title "System of Identification of Beneficiaries of the Social Programs" database or Sisben. Population parameters such as a priori kinship (phi(ii)), population homogeneity with B and C estimators, and Cavalli-Sforza's genetic distance were calculated for (a) three towns of Marinilla and its influence zone and (b) Aranzazu. The Rionegro population served as an external, comparison population. RESULTS: The Aranzazu and Marinilla populations showed the higher homogeneity (B value between 0.25 and 0.5) in contrast with Rionegro (B = 0.159), as well as greater a priori kinship values (4), between 0.003 and 0.010). The lowest distances were found between Marinilla and Aranzazu. CONCLUSIONS: Aranzazu is a population with characteristics similar to those of Marinilla and its influence zone. The close similarity of genetic characteristics for these populations is due probably to a founder effect. Furthermore, the genetic similarity predicts that genetic diseases will have the same etiology in both populations and provides optimum conditions for gene mapping studies.


Asunto(s)
Genética de Población , Dinámica Poblacional , Colombia/etnología , Humanos , Nombres , Grupos de Población
18.
Infect Genet Evol ; 38: 35-43, 2016 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-26631812

RESUMEN

Trypanosoma cruzi, the causative agent of Chagas disease, has been classified into six discrete typing units (DTUs) named TcI to TcVI. Furthermore, subcontinental scale studies based on analysis of the splice leader intergenic region (SL-IR) of the mini-exon gene have subdivided TcI in five genetic groups (Ia-Ie) related to the domestic and non-domestic cycles. However, a current review of this marker among all the sequences deposited in the GenBank demonstrates no correlation between the genetic structure and the eco-epidemiological features of parasite transmission. In this study, we performed a multilocus analysis of TcI isolates from a diverse array of hosts and vectors in a wide eco-geographical area of Colombia. Sequences from SL-IR and mitochondrial cyt b genes as well as PCR-RFLP profiles for four nuclear genes were analyzed. Multilocus analysis indicates that genetic structuration associated with sylvatic and domestic cycles in Colombia is not an attribute conserved across the entire eco-geography where TcI can be found.


Asunto(s)
Enfermedad de Chagas/epidemiología , Enfermedad de Chagas/parasitología , Tipificación de Secuencias Multilocus , Trypanosoma cruzi/clasificación , Trypanosoma cruzi/genética , Colombia/epidemiología , Citocromos b/genética , ADN Intergénico , ADN Protozoario , Variación Genética , Haplotipos , Filogenia , Polimorfismo de Nucleótido Simple , Análisis de Secuencia de ADN
19.
Biomedica ; 35(1): 53-61, 2015.
Artículo en Español | MEDLINE | ID: mdl-26148034

RESUMEN

INTRODUCTION: Aedes aegypti populations may experience changes in abundance and genetic diversity in addition to changes in their evolutionary capability to respond to vector control. The knowledge on the changes in genetic variation on a spatio-temporal scale improves the epidemiological understanding of dengue and supports the appropriate and timely design of vector control strategies. OBJECTIVE: To assess the genetic changes, diversity and gene flow in six microgeographical populations of Ae. aegypti in Medellín for different epidemiological periods of dengue. MATERIALS AND METHODS: A total of 255 specimens from six different neighborhoods in Medellín were used to assess variations in the CO1 mtDNA haplotype composition, diversity and genetic differentiation for an epidemic period (2010) and an endemic period (2012). RESULTS: Two groups of highly differentiated haplotypes were present in both periods, and a high-frequency haplotype was assessed for all neighborhoods. The highest haplotype diversity was recorded in 2012, but the maximum nucleotide diversity was recorded in 2010. No significant correlation between genetic and geographic distances was observed. CONCLUSIONS: The genetic composition of Ae. aegypti varies over time without a predictable pattern. In addition, the presence of a high-frequency haplotype in both periods could indicate a persistent variation adapted to vector control. However, the simultaneous movement of highly differentiated CO1 haplotypes compatible with multiple introductions suggests that different gene pools would be suitable for transmission. These results are consistent with mosquito dispersion due to human activities, which would enable the rapid spread of the virus during epidemics in Medellin.


Asunto(s)
Aedes/genética , Genes de Insecto , Variación Genética , Animales , Colombia , Demografía , Geografía , Haplotipos
20.
Biomedica ; 23(1): 87-102, 2003 Mar.
Artículo en Español | MEDLINE | ID: mdl-12696402

RESUMEN

The verrucarum group of phlebotomine sand flies includes vectors of Leishmania spp. and Bartonella bacilliformis, and from the perspective of public health is considered as one of the most important groups of neotropical phlebotomine sand flies. Due to marked morphological similarity among species constituting this group, the identification based on conventional taxonomic characters can be difficult. Consequently, the verrucarum group has been the focus of numerous taxonomic comparisons which have included the following methods: chaetotaxy, morphometry, larval spiracular system, chorionic structure, morphology of the genital atrium, cytogenetics, morphological phylogenetics, isoenzymes, random amplified polymorphic DNA, cuticular hydrocarbons, DNA probes, and nuclear and mitochondrial nucleotide sequences. Based on morphological characters of the male terminalia, the verrucarum group has been divided in four series, i.e., verrucarum, serrana, townsendi and pia. Since the revision of the group made by Young and Duncan in 1994, ten new species, principally of Andean origin, have been assigned to 3 of the series verrucarum (L. maranonensis, L. cajamarcensis, L. antioquiensis, L. falcaorum), serrana (L. robusta, L. guilvardae) and pia (L. suapiensis, L. tihuiliensis, L. tocaniensis, L. limafalcaoae). The total number of verrucarum group members is now 40. Explanations for this diversity of species include the isolation of ancestral populations in refugia of humid forest during the quaternary period, the Andean cordilleras as geographical barrier, and the appearance of the Isthmus of Panama. Biology systematics and evolution of the verrucarum group is reviewed with emphasis on the 19 species extant in Colombia.


Asunto(s)
Psychodidae/clasificación , Animales , Psychodidae/anatomía & histología
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