RESUMEN
BACKGROUND AND OBJECTIVES: Glycoproteins D (gD) and G (gG) of herpes simplex virus type 1 (HSV-1) are virus envelope glycoproteins that are able to induce HSV-1 antibody production in infected persons. Therefore, those proteins could be in interest to develop the serodiagnostic test(s) for HSV antibody detection. The aim of present study was the comparison of anti-gD and anti-gG antibodies in HSV-1 infected individuals' serum samples. MATERIALS AND METHODS: In this study, recombinant gD and gG were prepared and used for western blot test to detect the antibodies against HSV-1. RESULTS: Our data showed the total gD antibody titer was higher than gG antibody titer in the HSV-1 infected patient's sera but the gG antibody titer was high significantly. CONCLUSIONS: According to our results, gD and gG can be used for designing the diagnostic laboratory tests to evaluate total antibody against HSV-1 and HSV-2.
Asunto(s)
Anticuerpos Antivirales/sangre , Herpes Simple/diagnóstico , Herpesvirus Humano 1/inmunología , Proteínas del Envoltorio Viral/inmunología , Western Blotting , Células HeLa , Herpes Simple/sangre , Herpes Simple/virología , Humanos , Irán , Pruebas de Neutralización , Valor Predictivo de las Pruebas , Pruebas SerológicasRESUMEN
Much attention is presently focused on the vaccination with certain epitopes of an antigen. To further study the ability of neutralizing epitopes mapped in the first 1515 nucleotides of glycoprotein B of herpes simplex virus type-1 (gB-1) to induce neutralizing antibodies, a DNA immunization approach was employed. Vaccination of mice with a plasmid expressing the neutralizing epitopes induced humoral immune responses, although the antibody titre was significantly lower than that of antibodies induced by the full-length gB-1 gene. Furthermore, the plasmid DNA could not protect the mice against HSV-1 lethal challenge, but could significantly prolong the survival time compared to mock-vaccinated group.