Metals in Plant Functional Types of Ombrotrophic Peatlands in the Sudetes (SW Poland).

The Sudetes are remarkable for the variety and number of peat bogs which receive nutrients via precipitation from atmospheric deposition as the only source of minerals. As this type of peat bogs with a very low buffering capacity is affected in the Sudetes by long-range exhausts from the former Black Triangle, strong response to atmospheric contamination may be expected. Therefore these peat bogs are highly suitable for bioindication purposes. As a result, metal levels in peat and plants should be controlled to evaluate potential ecological damage and to devise treatment strategies. The aim of this study was to evaluate the concentration of Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, and Zn in species from different plant functional types (PFTs): shrubs, evergreen dwarf shrubs, deciduous dwarf shrubs, tussock sedges, non-tussock sedges, forbs, Sphagnum mosses, brown mosses, liverworts, and algae collected from peat bogs of the Izera, Karkonosze, and Bystrzyckie Mountains. PFTs of the Karkonosze peat bogs situated above the upper forest line contained higher metal concentrations than those of the Izera and Bystrzyckie peat bogs from lower altitudes and surrounded by forests. Of all PFTs, the algae Zygogonium ericetorum accumulated the highest levels of Fe, Pb, and Zn. The PFTs of Sphagnum mosses were also very effective bioindicators of Cd, Cr, Fe, Hg, and Pb deposition to peat bog ecosystems. Pb, Fe, and Cr found in the examined vascular PFTs originated from atmospheric deposition. The results showed that airborne contaminants, including the ones connected with long-range transport, can make a significant contribution to a load of trace metals in peat bogs located above the upper forest line. These airborne depositions facilitate better recognition of the transport of contaminants carried over great distances and should be taken into account in monitoring and environmental protection programs. In particular, the results, first of all, show the differences in the bioaccumulation of metals in PFTs and their response to trace metal levels in such habitats. Of all PFTs, algae and Sphagnum mosses were the best choices for bioindication of trace metal pollution in ombrotrophic mountain mires. PFTs have not been used so far for investigating ombrotrophic mountain mires in Europe. Thus results of this investigation could be extended to this type of peat bogs in the mountains of Central Europe for better selection of PFTs for bioindication purposes.

Trace elements in Athyrium distentifolium from alpine vegetation in the Karkonosze, SW Poland.

The Karkonosze National Park is affected by long-distance metal air transport of anthropogenic contamination as well as by tourist activity. Therefore, concentrations of Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb and Zn were evaluated in soil as well as in vital and non-vital fronds of Athyrium distentifolium Opiz collected in the middle of the growing season from glacial cirques in the Karkonosze. Additionally, fronds of the same species turning brown in autumn were collected from the same sampling sites. The health of ferns was impacted by the contents of Co, Cu, Fe, Mn, Ni, Pb and Zn in fronds, which may indicate that A. distentifolium uses elements accumulated in its tissues as defence against pathogens. Individuals from higher altitudes contained higher concentrations of Cd, Cu, Ni, Fe and Pb than those from lower altitudes. Autumn fronds of A. distentifolium contained a significantly higher concentration of Cr, Cu, Fe, Mn and Pb than vital summer fronds, which may indicate sequestration of these elements in senescing fronds probably to remove potentially harmful metals. Non-vital ferns were a better accumulator of Pb than vital ones, while both types of ferns accumulated Mn in a similar way.

Trace elements in Polytrichum commune and Polytrichastrum formosum from the Karkonosze Mountains (SW Poland).

The Karkonosze National Park, an unique mountainous biosphere reserve, is influenced by long-distance anthropogenic atmospheric transport of among others trace elements and additionally by local tourist centres, which may be supplementary sources of pollution. Discharged trace elements are non-degradable, and their level must be precisely monitored. Therefore, the concentrations of As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Mo, Ni, Pb, Rb and Zn in Polytrichum commune and Polytrichastrum formosum collected from the Karkonosze sites influenced only by long-range pollution and from sites in the vicinity of local tourist centres were established. P. commune and P. formosum revealed the ability to accumulate higher concentrations of trace elements while growing in locally contaminated sites in comparison with sites free from such pollution. Therefore, both species may be utilised for bioindication in the Karkonosze National Park. Elevated levels of trace elements in both species (except for Hg) in comparison with concentrations typical for mosses from unpolluted sites point to the existence of pollution of this area. P. commune was a better bioindicator of Cd, Co, Cr, Cu, Mo, Ni, Pb and Rb than P. formosum, probably because of the larger gametophytes and its morphology, which appears prone to an increased uptake of trace elements from the atmosphere.

Transplanted Moss Hylocomium splendens as a Bioaccumulator of Trace Elements from Different Categories of Sampling Sites in the Upper Silesia Area (SW Poland): Bulk and Dry Deposition Impact.

Concentrations of Cd, Co, Cr, Cu, Ni, K, Fe, Mn, Pb, V and Zn in transplants of Hylocomium splendens (Hedw.) Schimp. were compared with bulk deposition and dust samples from three different categories of sites: industrial, residential and their surroundings and rural (15 in total). Mosses were transplanted for 90 days to severely polluted areas of Upper Silesia, and samples of precipitation and dust were collected during the same period. Most of the significant correlations between element concentrations in mosses and bulk deposition (Cd, Ni, Pb and Zn) were found for industrial sites. In this study dry deposition carried higher element concentrations than bulk deposition, which may result in the higher passive capture of particulate matter by mosses.

Bioindication of PBDEs and PCBs by native and transplanted moss Pleurozium schreberi.

PBDEs and PCBs are toxic, persistent organic pollutants (POPs), and the use of PCBs is forbidden, but they are still present in many environments and biota. 90-day assays were conducted with the moss Pleurozium schreberi transplanted from an uncontaminated control site to ten sites (rural and urban) selected in one of the most polluted regions of Upper Silesia in Poland. Native P. schreberi mosses were collected from the same ten polluted sites. Concentrations of PBDEs (28, 47, 66, 85, 99, 100, 153, 154, 183 and 209) and PCBs (28, 52, 101, 118, 138, 153, 180) were determined in all native and transplanted P. schreberi from all sites. Native P. schreberi contained the highest ΣPBDE and ΣPCB levels (63.6ngg-1 and 4.47pgg-1, respectively) when collected in the vicinity of a steel smelter. After 90 days of the experiment native and transplanted P. schreberi contained the highest concentrations of the same BDE 209 congener (88-91% of total PBDEs in the native mosses and 85-90% of the total PBDE burden in the transplants). The native and transplanted mosses from the industrial sites after 90 days of exposure contained significantly higher concentrations of all the examined PBDE and PCB congeners (except for 153 and 180) than mosses from rural sites. PBDE and PCB values were higher in native than in transplanted mosses after 90 days of exposure in both rural and industrial sites.

δ(34)S values and S concentrations in native and transplanted Pleurozium schreberi in a heavily industrialised area.

Sulphur is an element found in surplus in anthropogenic areas and one of the minerals responsible for the development of acid rains. The analysis of stable S isotopes provides a powerful tool for studying various aspects of the biogeochemical circulation of sulphur. δ(34)S values and S concentrations were determined in a 90-day experiment with the native moss Pleurozium schreberi from rural, urban and industrial sites in Upper Silesia in southern Poland. At the same time P. schreberi from a control site was transplanted to the same rural, urban and industrial sites and the δ(34)S values and S concentrations were determined in the same 90-day experiment. (34)S enrichment (up to 4.7‰) in the mosses tested indicates that these plants responded to environmental pollution stress. Sulphur isotopic composition in the transplanted P. schreberi was related to S concentrations in this species after 90 days of the experiment. Higher δ(34)S values and S concentrations were noted in native mosses than in those transplanted from rural and urban sites while an opposite situation was reported in industrial sites. The transplanted P. schreberi was a better sulphur bioindicator than the native moss in more polluted industrial sites and worse in less polluted rural and urban sites.

Polybrominated diphenyl ethers (PBDEs) in herbaceous Centaurium erythraea affected by various sources of environmental pollution.

Polybrominated diphenyl ethers (PBDEs) are persistent xenobiotics with harmful effects on humans and wildlife. Their levels in the environment and accumulation in biota must be carefully controlled especially in species harvested from wild populations and commonly used as medicines. Our objective has been to determine PBDE concentrations (BDEs 28, 47, 66, 85, 99, 100, 153, 154, 183 and 209) in Centaurium erythraea collected at sites with various levels of environmental pollution. PBDE congener profiles in C. erythraea were dominated by BDE209, which accounted for 47-89% of the total PBDE burden in the plants. Principal Component and Classification Analysis, which classifies the concentration of PBDEs in C. erythraea, allowed us to distinguish the pattern of these compounds characteristic for the origin of pollution: BDEs 28, 47, 66, 85, 99, 100 for lignite and general chemical industry and the vicinity of an expressway and BDEs 183 and 209 for a thermal power plant and ferrochrome smelting industry. Careful selection of sites with C. erythraea for medicinal purposes is necessary as this herb can accumulate PBDEs while growing at polluted sites.

Mercury in Pleurozium schreberi and Polytrichum commune from areas with various levels of Hg pollution--an accumulation and desorption experiment with microscopic observations.

Because of its high mobility in ecosystems, mercury is one of the main toxic threats to the environment, and its concentration must be carefully controlled. To fulfill this need, we selected terrestrial mosses with different characteristic life forms: orthotropic and endohydric Polytrichum commune and plagiotropic and ectohydric Pleurozium schreberi. The concentrations of mercury were determined in both species growing together at sites situated approximately 0.75, 1.5, 3 and 6km to the north, south, east and west, respectively of five known mercury polluters. The mercury concentrations reflected the emissions produced by the surrounding industry, reaching values of 0.44mgkg(-1) in P. schreberi and 0.79mgkg(-1) in P. commune in the vicinity of the chlor-alkali industry. To determine how long a load of Hg would remain in the mosses after mercury emitters restricted releases of Hg to the atmosphere, accumulation and desorption experiments were performed. We compared the two moss species collected from clean and moderately and heavily mercury-polluted sites. After eight days of exposure to mercury, P. schreberi accumulated up to 25mgkg(-1) of Hg, and P. commune accumulated up to 31mgkg(-1). Both in the field and in the experiment, P. commune accumulated significantly higher concentrations of Hg than did P. schreberi, most likely because of its surface morphology, which is likely to enhance the capture of metal from the atmosphere. After sixteen days of exposure, mercury changed the structure of the plasma membrane and affected organelles such as the nuclei and chloroplasts, leading to cell disintegration and death. The negative effects of mercury on the functioning of living cells appeared first in the older leaves of P. schreberi. After 64 days growing in the absence of Hg, P. schreberi clearly retained only 10-14% of the initially accumulated Hg, while P. commune retained 10-21%.

Tanacetum vulgare as a bioindicator of trace-metal contamination: a study of a naturally colonized open-pit lignite mine.

We investigated the possibility of use of Tanacetum vulgare (tansy) as an ecological indicator of metal concentration in a naturally colonized open-pit lignite mine in Belchatów (Poland). Tanacetum vulgare is the only species growing abundantly and spontaneously in the lignite mine waste dumps. Metal concentrations in roots, stems, leaves, flowers, and soil were measured in dump sites differing in type and time of reclamation and therefore differing in pollution levels. Tanacetum vulgare appeared to be an accumulator of chromium and iron in roots, whereas highest concentrations of manganese and zinc were found in leaves. A high bioaccumulation factor for cadmium (Cd) was observed in dumps and control sites, indicating that even small amounts of Cd in the environment may result in significant uptake by the plant. The lowest concentrations of metals were found in plants from sites situated on dumps reclaimed with argillaceous limestone.

Andromeda polifolia and Oxycoccus microcarpus as pollution indicators for ombrotrophic bogs in the Western Sudety Mountains (SW Poland).

Concentrations of the elements Cd, Cr, Cu, Fe, Li, Mn, N, Ni, Pb and Zn in Andromeda polifolia, Oxycoccus microcarpus and in the peat in which these plants grew were measured in the Western Sudety (Karkonosze and Izerskie Mts., SW Poland). Of both the investigated plant fruit, O. microcarpus harvested from wild populations are commonly used as medicines. Samples from ombrotrophic bogs were investigated within the area influenced by exhausts of the former Black Triangle, one of the most heavily industrialized and polluted areas in Europe. A. polifolia and O. microcarpus growing at the highest elevations contained the highest Cu, Li, Ni, Mn and Zn concentrations and in addition O. microcarpus also contained the highest Cr concentrations. Both the investigated species have wide circumpolar distribution in ombrotrophic mires of the Northern hemisphere. As this type of mires is nourished solely by atmospheric deposition, the increased metal concentrations in A. polifolia and O. microcarpus may be an indication that their habitats receive an atmospheric input of long-range transported pollution. Our investigation proves that both species are able to accumulate elevated metal levels and may be used in the bioindication of the metal status in ombrotrophic mires. Controlling the collection of O. microcarpus fruit for consumption and medicinal purposes is recommended as this species can accumulate increased metal levels. However, further more detailed studies are necessary to verify the inner translocation of metals into fruit.

Viscum album versus host (Sorbus aucuparia) as bioindicators of urban areas with various levels of pollution.

In this investigation we focus on the concentration of elements in Viscum album and its host (Sorbus aucuparia) as bioindicators of urban pollution. These broadly widespread species, very common in polluted areas may provide important information to monitor environmental quality throughout the year, especially for V. album. Cd, Co, Cr, Cu, Fe, Li, Mg, Mn, Ni, Pb and Zn concentrations were measured in the leaves and soil of the tree S. aucuparia as well as in V. album, a semi-parasite living on this tree species. The tree and the semi-parasite were studied in the urban environment of Olawa (SW Poland). This area was selected because of the influence of a zinc smelter on the level of metal pollution of soil and plants and to compare the ability of S. aucuparia and V. album to accumulate metals. V. album appeared to be a better bioaccumulator of Cd, Ni, Pb and Zn and a weaker accumulator of Co than S. aucuparia in less polluted sites of Olawa. S. aucuparia was a better bioaccumulator of Cd, Ni, Pb and Zn and a weaker accumulator of Co than V. album in more polluted sites. Cluster analysis of Cd, Co, Cr, Cu, Fe, Li, Mg, Mn, Ni, Pb and Zn concentrations in plants distinguished sites with lower and higher pollution levels which suggests a possibility of using these species for bioindication. However, the ratio of metals in V. album to S. aucuparia was different depending on the pollution level.

Metals in Callitriche cophocarpa from small rivers with various levels of pollution in SW Poland.

The anthropogenic impact of metals on aquatic environments is a risk for biota, and thus their levels must be controlled. Callitriche cophocarpa Sendtn. belongs to a genus with a potential for accumulation of elevated metal levels. Thus, it may provide consolidated evidence of contamination. Therefore, the aim of this investigation was to determine Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb and Zn in this species collected together with water and bottom sediments from rivers with various levels of pollution. Of these rivers, one less polluted and one more polluted was selected for the collection of C. cophocarpa for an experiment to compare its Cu and Zn concentration potential. Both metals were supplemented at concentrations 0.01, 0.02, 0.03, 0.05, 0.08 and 0.14 mg L-1 of Cu as CuSO4 × 5H2O and 0.4, 0.6, 0,9, 1,35, 2.03 and 3.04 mg L-1 of Zn as ZnSO4 × 7H2O, and in the binary design containing (mg·L-1) 0.01Cu + 0.4Zn, 0.02Cu + 0.6Zn, 0.03Cu + 0.9Zn, 0.05Cu + 1.4Zn, 0.08Cu + 2.03 Zn and 0.14Cu + 3.04Zn. The upper concentrations of Cr, Cu, Mn and Zn in C. cophocarpa shoots from both types of rivers as well as of Ni and Pb in shoots from more polluted rivers were higher than the values typical for toxicity thresholds with no visible harmful effects, which may indicate accumulation abilities of C. cophocarpa for these metals. Both roots and shoots of C. cophocarpa may be included in the group of macroconcentrators for bottom sediments with respect to Cd, Co, Cr, Cu, Fe, Mn, Ni and Zn and deconcentrators of Pb. Greater accumulation of most metals in roots than in shoots indicates their restricted mobility and translocation by C. cophocarpa to shoots. C. cophocarpa from the less polluted river and exposed to all experimental solutions contained significantly higher levels of Cu and Zn than that from the more polluted river exposed to identical experimental solutions. The plants collected from the more polluted river influenced by surplus of metals and living under chemical stress could probably limit further accumulation by developing a resistance mechanism. Cu and Zn contents in C. cophocarpa were higher when treated with separate metals than for binary treatment both in the more and less polluted river. Such research presenting the impact of a combination of metals could be important for understanding and explaining the interactions of these elements which may influence their bioavailability in nature as well as importance in the evaluation of the risk of environmental toxicity.

Athyrium distentifolium used for bioindication at different altitudes in the Tatra National Park (South Poland).

This research is a continuation of investigations on the biogeochemistry of metal accumulation of the fern Athyrium distentifolium in the Tatra National Park in Poland. This species, abundantly growing throughout the Tatra mountains, was sampled from sites with identical type of bedrocks (granites/gneisses) at various altitudes between 1000 and 2050 m a.s.l. Concentrations of the elements Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn were measured in the fern. This investigation was done to verify the hypothesis that differences in the elemental composition of A. distentifolium (growing on the same type of substrate) reflect the differences of the atmospheric metal deposition at various altitudes. We verified this hypothesis using the Market Basket Analysis technique. MBA revealed that at low altitude (1000-1500 m a.s.l.) A. distentifolium contains very low concentrations of Cd, Fe, Mn, Ni, Pb and Zn. At a medium altitude (1501-1700 m a.s.l.) the fern contains a very high concentration of Pb and at a high altitude (1701-2050 m a.s.l.) the fern contains very high concentrations of Cd, Cr, Mn, Ni and Zn. The different altitudes on which A. distentifolium grew influenced the concentrations of elements accumulated in this species. Our investigation pointed out that A. distentifolium is able to accumulate elevated levels of metals and therefore may be used in controlling metal pollution. In addition A. distentifolium gives comparable bioindicative results as the moss P. schreberi in the same Tatra mountains. As the investigated species has a wide circumpolar distribution in mountains of the Northern hemisphere therefore it may be used in controlling long range metal pollution in such mountainous areas.

Metal contents in Centaurium erythraea and its biometry at various levels of environmental pollution.

In this investigation we focus on the influence of pollution on concentration of elements in the medicinal Centaurium erythraea. This herb is collected from wild populations and also provides important information as monitor of environmental quality. Concentrations of Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, N, Ni, P, Pb, V and Zn in Centaurium erythraea and in the soil in which this plant grew were measured together with biometrical features of the species from sites affected by various levels of pollution. C. erythraea from sites polluted by lignite mining and industrial combustion had the highest concentrations of Cd, Co and Zn (principal component and classification analysis, PCCA). C. erythraea from sites influenced by ferrous-chromium and thermal power plants had the highest concentrations of Cr and Fe as well as Cu, Mn and Ni and the highest length of shoots and number of leaves, shoots, nodes, flowers and flowers on the main shoot. These luxurious growth forms were probably related to the highest concentrations of N, P and Fe in the soil and concentrations of N and K accumulated by C. erythraea on these sites as revealed by the established models. Controlling the collection of C. erythraea for medicinal purposes is recommended as this species is able to accumulate increased levels of metals from polluted sites. The medical quality of C. erythraea may be negatively related to the degree and type of pollution of the environment and should not be based on the luxurious growth of individuals which may contain hazardous levels of metals.

Bioindicative comparison of the fern Athyrium distentifolium for trace pollution in the Sudety and Tatra mountains of Poland.

Concentrations of the elements Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn were measured in the fronds of the fern Athyrium distentifolium from the Sudety and Tatra mountains (Poland). The A. distentifolium sites in the Sudety mountains which were influenced by long-range metal transport from the former Black Triangle were distinguished by the principal component and classification analysis (PCCA). These sites were situated on the west side slopes of one of the ranges in the Sudety mountains (within a 150-km radius of the heart of the former Black Triangle) at an altitude of 700 m asl, and exposed to prevailing winds. This most affected area had significantly higher foliar concentrations of Cu, Cr and Ni which are typical for long-range transported airborne elements occurring in coal fly ash emitted by lignite combustion industry.

Metals in Calluna vulgaris, Empetrum nigrum, Festuca vivipara and Thymus praecox ssp. arcticus in the geothermal areas of Iceland.

This investigation was conducted to identify the content of metals in Calluna vulgaris (family Ericaceae), Empetrum nigrum (family Ericaceae), Festuca vivipara (family Poaceae) and Thymus praecox subsp. arcticus (family Lamiaceae), as well as in the soils where they were growing in eight geothermal heathlands in Iceland. Investigation into the vegetation of geothermal areas is crucial and may contribute to their proper protection in the future and bring more understanding under what conditions the plants respond to an ecologically more extreme situation. Plants from geothermally active sites were enriched with metals as compared to the same species from non-geothermal control sites (at an average from about 150 m from geothermal activity). The enriched metals consisted of Cd, Co, Cu, Fe and Ni in C. vulgaris; Cd, Mn and Ti in E. nigrum; Hg and Pb in F. vivipara; and Cd, Fe and Hg in T. praecox. Notably, C. vulgaris, E. nigrum, F. vivipara and T. praecox had remarkably high concentrations of Ti at levels typical of toxicity thresholds. Cd and Pb (except for C. vulgaris and F. vivipara) were not accumulated in the shoots of geothermal plants. C. vulgaris from geothermal and control sites was characterised by the highest bioaccumulation factor (BF) of Ti and Mn; E. nigrum and F. vivipara by the highest BF of Ti and Cr; and T. praecox by the highest BF of Ti and Zn compared to the other elements. In comparison with the other examined species, F. vivipara from geothermal sites had the highest concentration of Ti in above-ground parts at any concentration of plant-available Ti in soil.

Market Basket Analysis: a new tool in ecology to describe chemical relations in the environment--a case study of the fern Athyrium distentifolium in the Tatra National Park in Poland.

In this study, the novel data mining technique Market Basket Analysis (MBA) was applied for the first time in biogeochemical and ecological investigations. The method was tested on the fern Athyrium distentifolium, in which we measured concentrations of the elements Ca, Cd, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, and Zn. Plants were sampled from sites with different types of bedrock in the Tatra National Park in Poland. MBA was used to investigate whether specimens of Athyrium distentifolium that contain elevated levels of certain elements occur more frequently on a specific type of bedrock and to identify relationships between the type of bedrock and the concentrations of the elements in this fern. The results were compared with those of the commonly used principal component and classification analysis (PCCA) technique. MBA and PCCA ordination both yielded distinct groups of ferns growing on different types of bedrock. Although the results of MBA and PCCA were similar, MBA has the advantage of being independent of the size of the data set. In addition, MBA revealed not only dominant elements but, in the case of limestone bedrock, also showed very low concentrations of Cd, Fe, Mn, and Pb in ferns growing on this type of parent material. MBA, thus, appeared to be a promising data mining method to reveal chemical relations in the environment as well as the accumulation of chemical elements in bioindicators. This technique can be used to reveal associations and correlations among items in large data sets collected on a national or even larger scale.

Cobalt and nickel content in Hydrocharis morsus-ranae and their bioremoval from single- and binary solutions.

Aquatic macrophytes are known to remove trace metals from surrounding water. In the present study, an attempt was made to evaluate the phytofiltration capacity of Hydrocharis morsus-ranae and to show competition between cobalt (Co) and nickel (Ni) for the better understanding of metal bioaccumulation in the species. In a laboratory experiment, H. morsus-ranae was exposed to separate (single) and binary solutions of these metals: Ni 10.7, 18.7, 32.7, 57.1, and 100 (µg L-1); Co 5.33, 9.32, 16.3, 28.6, and 50.0 (µg L-1); and 10.7 Ni + 5.33 Co, 18.7 Ni + 9.32 Co, 32.7 Ni + 16.3 Co, 57.1 Ni + 28.6 Co, 100 Ni + 50.0 Co (µg L-1). The content of Co and Ni in the plant increased with the increasing concentration in the growth medium. Competition between the metals was seen during uptake in binary solutions. Ni interfered with the accumulation of Co, resulting in a lower Co content than in plants cultivated in Co solutions. A particularly high Co content (up to 155 mg kg-1 dry weight [d.w.]) and high efficiency of Ni uptake (Bioaccumulation Factor (BF) 2572-7239) makes the species a very good accumulator of these metals. The high content of both trace metals in plant tissues (up to 511 mg kg-1 d.w. Ni and 155 mg kg-1 d.w. Co) did not affect its growth, indicating tolerance of these toxicants. The plant showed excellent ability in removing Co (up to 98.6% in solution with 5.33 µg L-1 Co) and Ni (up to 91.4% in solution with 57.1 µg L-1 Ni and 28.6 µg L-1 Co) from nutrient solution. The results suggest that H. morsus-ranae may be useful for the phytoremediation of water bodies contaminated with Co and Ni.

Metals in Racomitrium lanuginosum from Arctic (SW Spitsbergen, Svalbard archipelago) and alpine (Karkonosze, SW Poland) tundra.

Arctic-alpine tundra habitats are very vulnerable to the input of relatively small amounts of xenobiotics, and thus their level in such areas must be carefully controlled. Therefore, we collected the terrestrial widespread moss Racomitrium lanuginosum (Hedw.) Brid. in Spitsbergen in the Arctic moss lichen tundra and, for comparison, in the Arctic-alpine tundra in the Karkonosze (SW Poland). Concentrations of the elements Cd, Co, Cr, Cu, Fe, Hg, Li, Mn, Mo, Na, Ni, Pb, V, and Zn in this species and in the parent rock material were measured. We tested the following hypothesis: R. lanuginosum from Spitsbergen contains lower metal levels than the species from the Karkonosze collected at altitudes influenced by long-range transport from former Black Triangle industry. Principal component and classification analysis (PCCA) ordination revealed that mosses of Spitsbergen were distinguished by a significantly higher Na concentration of marine spray origin and mosses of Karkonosze were distinguished by significantly higher concentrations of Cd, Cr, Cu, Fe, Hg, Li, Mn, Pb, V, and Zn probably from long-range atmospheric transport. The influence of the polar station with a waste incinerator resulted in significantly higher Co, Li, and Ni concentrations in neighbouring mosses in comparison with this species from other sites. This investigation contributes to the use of R. lanuginosum as a bioindicator for metal contamination in Arctic and alpine tundra regions characterised by severe climate habitats with a restricted number of species. This moss enables the control of pollution usually brought solely by long-range atmospheric transport in high mountains as well as in Arctic areas.

The coupled study of metal concentrations and electron paramagnetic resonance (EPR) of lichens (Hypogymnia physodes) from the Swietokrzyski National Park-environmental implications.

SO2, NOx, and metals (including Cd, Cu, Pb, Zn, Mn, Mg, Fe) present in airborne particulate matter are a major threat to preserving good air quality. The complicated pathways and transformation processes that can change their physical/chemical state in the atmosphere renders identifying their origin extremely difficult. With the objective of alleviating this difficulty, we identified and characterized potential local and regional sources of atmospheric pollutants using bioindicators (Hypogymnia physodes) from the Swietokrzyski National Park (SE Poland): 20 lichen samples were collected during winter (February; heating period) and summer (June; vegetative period) seasons and analyzed for metal contents and free radicals concentrations. Our results indicate that the highest gaseous pollutant levels were observed during the heating season, along roads (NO2) and at the highest elevation (SO2). The semiquinone/phenoxyl radical concentrations correlated during the heating season with the atmospheric SO2: ln (free radicals concentrations) = 0.025 SO2atmosphere + 39.11. For Mn/Fe ≥ 2, the electron paramagnetic resonance (EPR) spectra presented a hyperfine splitting. Results showed that since 1994 metal concentrations increased for Cd, Mn, and Mg, Fe remained somewhat constant for Zn and Cu but slightly decreased for Pb, in agreement with the phasing out of lead in gasoline. Finally, a principal component analysis (PCA) identified two main factors controlling variability within the analyzed parameters: air pollutants transport over long distances and local fuel combustion by both transport and home heating.