Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Más filtros

País/Región como asunto
Tipo del documento
País de afiliación
Intervalo de año de publicación
1.
Braz J Infect Dis ; 22(3): 202-207, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29787713

RESUMEN

INTRODUCTION: Latent tuberculosis infection diagnosis based on the release of interferon-gamma in cultures of peripheral blood cells stimulated with Mycobacterium tuberculosis antigens has replaced the tuberculin skin test in many countries with low tuberculosis prevalence. The IFN-γ production can be influenced by genetic polymorphisms, of which the IFNG+874 (rs62559044) locus is the most studied. We investigated the possible influence of the IFNG+874 A/T polymorphism on interferon-gamma test performance. METHODS: Patients diagnosed with pulmonary tuberculosis (75), volunteers with positive tuberculin skin test (70) and healthy volunteers with negative tuberculin skin test and no history of contact with tuberculosis (57) were evaluated regarding the IFNG+874 genotype and the IFN-γ levels in whole blood cultures performed using an interferon-gamma commercial kit (QuantiFERON-TB® Gold In-Tube). RESULTS: IFN-γ production was not influenced by the IFNG+874 genotype, regardless of antigen or mitogen-based stimulation, which suggests that other genes may influence IFN-γ production in response to mycobacteria. The IFNG+874 polymorphism was found to exert no influence over QFT-IT test sensitivity in our study. CONCLUSIONS: The IFNG+874 polymorphism was not shown to influence QuantiFERON-TB® Gold In-Tube test performance in an admixed population from northeastern Brazil.


Asunto(s)
Ensayos de Liberación de Interferón gamma/métodos , Interferón gamma/genética , Mycobacterium tuberculosis/genética , Polimorfismo Genético/genética , Tuberculosis Pulmonar/diagnóstico , Brasil , Estudios de Casos y Controles , Femenino , Frecuencia de los Genes , Genotipo , Técnicas de Genotipaje , Humanos , Interferón gamma/metabolismo , Masculino , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Estadísticas no Paramétricas , Prueba de Tuberculina
2.
Braz. j. infect. dis ; 22(3): 202-207, May-June 2018. tab, graf
Artículo en Inglés | LILACS | ID: biblio-974206

RESUMEN

ABSTRACT Introduction Latent tuberculosis infection diagnosis based on the release of interferon-gamma in cultures of peripheral blood cells stimulated with Mycobacterium tuberculosis antigens has replaced the tuberculin skin test in many countries with low tuberculosis prevalence. The IFN-γ production can be influenced by genetic polymorphisms, of which the IFNG + 874 (rs62559044) locus is the most studied. We investigated the possible influence of the IFNG + 874 A/T polymorphism on interferon-gamma test performance. Methods Patients diagnosed with pulmonary tuberculosis (75), volunteers with positive tuberculin skin test (70) and healthy volunteers with negative tuberculin skin test and no history of contact with tuberculosis (57) were evaluated regarding the IFNG + 874 genotype and the IFN-γ levels in whole blood cultures performed using an interferon-gamma commercial kit (QuantiFERON-TB® Gold In-Tube). Results IFN-γ production was not influenced by the IFNG + 874 genotype, regardless of antigen or mitogen-based stimulation, which suggests that other genes may influence IFN-γ production in response to mycobacteria. The IFNG + 874 polymorphism was found to exert no influence over QFT-IT test sensitivity in our study. Conclusions The IFNG + 874 polymorphism was not shown to influence QuantiFERON-TB® Gold In-Tube test performance in an admixed population from northeastern Brazil.


Asunto(s)
Humanos , Masculino , Femenino , Polimorfismo Genético/genética , Tuberculosis Pulmonar/diagnóstico , Interferón gamma/genética , Ensayos de Liberación de Interferón gamma/métodos , Mycobacterium tuberculosis/genética , Brasil , Prueba de Tuberculina , Estudios de Casos y Controles , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Interferón gamma/metabolismo , Estadísticas no Paramétricas , Técnicas de Genotipaje , Frecuencia de los Genes , Genotipo
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA