Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 210
Filtrar
Más filtros

Bases de datos
Tipo del documento
Intervalo de año de publicación
1.
Med Vet Entomol ; 33(3): 345-351, 2019 09.
Artículo en Inglés | MEDLINE | ID: mdl-30734975

RESUMEN

Invasive Aedes mosquito species (Diptera: Culicidae) are of public health concern in Europe because they are either recognized or potential vectors of pathogens. Loop-mediated isothermal amplification (LAMP) is a rapid and simple method for amplifying DNA with high specificity and efficiency, with the technique having potential for application in the field, including in high-throughput format. Specific LAMP assays based on rDNA internal transcribed spacers 1 or 2 sequences, considering intraspecies variability at these loci, were developed for Aedes aegypti, Aedes albopictus, Aedes japonicus, Aedes koreicus and the indigenous Aedes geniculatus. No such assays could be developed for Aedes atropalpus and Aedes triseriatus because both loci were too short to serve as target. The assays rely on the clearly visible colour change from violet to sky blue after successful amplification. Sensitivity of egg detection was confirmed with ratios of up to one mosquito egg in 99 other eggs. Simple sample preparation of adults or eggs by mechanical homogenization in water required an additional heat treatment or centrifugation step to avoid non-specific colour changes. Thus, further technical improvements are needed to render these assays truly field-applicable, which would greatly facilitate surveillance of these invasive mosquito species and allow for prompt implementation of control measures.


Asunto(s)
Aedes/clasificación , Mosquitos Vectores/clasificación , Técnicas de Amplificación de Ácido Nucleico/métodos , Aedes/genética , Animales , ADN Espaciador Ribosómico/análisis , Especies Introducidas , Mosquitos Vectores/genética
2.
Med Vet Entomol ; 33(4): 443-452, 2019 12.
Artículo en Inglés | MEDLINE | ID: mdl-31361038

RESUMEN

Mosquito-borne diseases resulting from the expansion of two key vectors, Aedes aegypti and Aedes albopictus (Diptera: Culicidae), continue to challenge whole regions and continents around the globe. In recent years there have been human cases of disease associated with Chikungunya, dengue and Zika viruses. In Europe, the expansion of Ae. albopictus has resulted in local transmission of Chikungunya and dengue viruses. This paper considers the risk that Ae. aegypti and Ae. albopictus represent for the U.K. and details the results of mosquito surveillance activities. Surveillance was conducted at 34 points of entry, 12 sites serving vehicular traffic and two sites of used tyre importers. The most common native mosquito recorded was Culex pipiens s.l. (Diptera: Culicidae). The invasive mosquito Ae. albopictus was detected on three occasions in southern England (September 2016, July 2017 and July 2018) and subsequent control strategies were conducted. These latest surveillance results demonstrate ongoing incursions of Ae. albopictus into the U.K. via ground vehicular traffic, which can be expected to continue and increase as populations in nearby countries expand, particularly in France, which is the main source of ex-continental traffic.


Asunto(s)
Aedes , Distribución Animal , Especies Introducidas , Mosquitos Vectores , Animales , Virus Chikungunya , Virus del Dengue , Control de Mosquitos , Reino Unido
3.
Med Vet Entomol ; 32(2): 175-185, 2018 06.
Artículo en Inglés | MEDLINE | ID: mdl-29424446

RESUMEN

Pathogens of medical or veterinary significance that are transmitted by mosquitoes (Diptera: Culicidae) are (re-)emerging in Europe [e.g. West Nile virus (WNV), Dirofilaria nematodes]. Little is known about the spatiotemporal abundances of mosquito species in Switzerland. Therefore, mosquito population dynamics were investigated, focusing on areas of risk for sylvatic or synanthropic transmission, such as natural sites and suburban sites on either side of the Alpine crest. Repeated collections were made using Centers for Disease Control (CDC) traps, juvenile sampling and ovitrapping. A total of 122 831 mosquito specimens of 21 taxa were identified. Levels of mosquito species richness were similar at suburban sites and in natural zones in Switzerland. Mosquito abundances and seasonality were analysed with generalized linear mixed models based on 382 CDC trap samples (29 454 females) and revealed Aedes annulipes/cantans, Aedes geniculatus, Aedes japonicus, Aedes sticticus, Aedes vexans, Coquillettidia richiardii and Culex pipiens/torrentium as the dominant species overall. Abundances of these species were season-dependent in most cases. There was an effect of site with regard to abundance (higher in natural zones), but not with respect to seasonality. Together with data on vector competence and the host preferences of different species, the present data contribute to assessments of risk for pathogen transmission. For example, both natural and suburban environments seem feasible as sites for amplification cycles of WNV and transmission to mammals.


Asunto(s)
Clima , Culicidae/fisiología , Mosquitos Vectores/fisiología , Animales , Infecciones por Arbovirus/transmisión , Arbovirus/fisiología , Femenino , Dinámica Poblacional , Estaciones del Año , Suiza
4.
Med Vet Entomol ; 31(1): 102-106, 2017 03.
Artículo en Inglés | MEDLINE | ID: mdl-27775162

RESUMEN

The Asian tiger mosquito Stegomyia albopicta (= Aedes albopictus) (Diptera: Culicidae), native to Asian forests, is a nuisance mosquito and is responsible for the transmission of arboviruses of public health importance, such as dengue, chikungunya and Zika viruses. It has colonized parts of all continents, except Antarctica, over the past 30-40 years. However, to date, the only records of S. albopicta in North Africa refer to occasional collections in 2010 and 2014 in Algeria. In early September 2015, S. albopicta larvae and adults were collected in a district of Rabat, Morocco. Morphological identification was confirmed by molecular analysis. This is the first record of this invasive mosquito in Morocco. A national surveillance programme will be implemented in 2016 to establish its geographical distribution in Morocco and to instigate control measures to prevent the establishment of new populations and the transmission of arboviruses.


Asunto(s)
Aedes/fisiología , Distribución Animal , Insectos Vectores/fisiología , Aedes/crecimiento & desarrollo , Animales , Femenino , Larva/genética , Larva/fisiología , Masculino , Marruecos , Salud Pública
5.
Med Vet Entomol ; 30(1): 64-72, 2016 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-26685872

RESUMEN

The efficacy of Centers for Disease Control (CDC) miniature light traps and ovitraps was tested in the outskirts of the city of Zurich in Switzerland for their use in the surveillance of Aedes (Hulecoeteomyia) japonicus japonicus (Theobald) (Diptera: Culicidae), the invasive Asian bush mosquito. Sets of single CDC traps were run overnight (n = 18) in three different environments (forest, suburban and urban) in 3 × 3 Latin square experimental designs. Traps were baited with: (a) carbon dioxide (CO2 ); (b) CO2 plus light, or (c) CO2 plus lure blend [Combi FRC 3003 (iGu® )]. At the same locations, mosquito eggs were collected weekly using standard ovitraps baited with different infusions (oak, hay or tap water) and equipped with different oviposition substrates (a block of extruded polystyrene, a germination paper strip or a wooden stick). Data were analysed using Poisson and negative binomial general linear models. The use of light (P < 0.001) or lure (P < 0.001) significantly increased the attractiveness of CDC traps baited with CO2 . Oak and hay infusions did not increase the attractiveness of ovitraps compared with standing tap water (P > 0.05), and extruded polystyrene blocks were preferred as an oviposition substrate over wooden sticks (P < 0.05) and seed germination paper (P < 0.05). Carbon dioxide-baited CDC miniature light traps complemented with light or iGu® lure and ovitraps containing standing tap water and polystyrene oviposition blocks can be considered as efficient and simple tools for use in Ae. j. japonicus surveillance programmes.


Asunto(s)
Aedes/fisiología , Control de Mosquitos/instrumentación , Control de Mosquitos/métodos , Feromonas/farmacología , Animales , Femenino , Masculino , Suiza
6.
Med Vet Entomol ; 30(1): 39-52, 2016 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-26685926

RESUMEN

The avian zoonotic agent for West Nile virus (WNV) can cause neuroinvasive disease in horses and humans and is expanding its range in Europe. Analyses of the risk for transmission to these hosts in non-endemic areas are necessary. Host preferences of mosquitoes (Diptera: Culicidae), the main vectors of WNV, were determined in Switzerland using animal-baited trap (horse, chickens) experiments at a natural and a periurban site. This was undertaken on four occasions during May-September 2014. In addition, the hosts of 505 blood-fed mosquitoes collected in a zoo and in the field were determined. Mosquito data obtained in the animal bait experiments were corrected for host weight and body surface area and by Kleiber's scaling factor. Collections of 11-14 different mosquito species were achieved with these approaches. Statistically significant host preferences were identified in three species in both approaches. The other species showed opportunistic feeding behaviours to varying extents. Specifically, the invasive species Hulecoeteomyia japonica (= Aedes japonicus) was identified for the first time as feeding on avians in nature. Abundance data, spatiotemporal activity and laboratory vector competence for WNV suggested that, in addition to the main WNV vector Culex pipiens, H. japonica and Aedimorphus vexans (= Aedes vexans) are the most likely candidate bridge vectors for WNV transmission in Switzerland.


Asunto(s)
Pollos , Culicidae/fisiología , Cadena Alimentaria , Caballos , Interacciones Huésped-Parásitos , Insectos Vectores/fisiología , Animales , Ciudades , Conducta Alimentaria , Femenino , Humanos , Masculino , Parques Recreativos , Suiza , Fiebre del Nilo Occidental/transmisión , Fiebre del Nilo Occidental/virología , Virus del Nilo Occidental/fisiología , Humedales
7.
Bull Entomol Res ; 105(6): 637-63, 2015 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-25804287

RESUMEN

Among the invasive mosquitoes registered all over the world, Aedes species are particularly frequent and important. As several of them are potential vectors of disease, they present significant health concerns for 21st century Europe. Five species have established in mainland Europe, with two (Aedes albopictus and Aedes japonicus) becoming widespread and two (Ae. albopictus and Aedes aegypti) implicated in disease transmission to humans in Europe. The routes of importation and spread are often enigmatic, the ability to adapt to local environments and climates are rapid, and the biting nuisance and vector potential are both an ecomonic and public health concern. Europeans are used to cases of dengue and chikungunya in travellers returning from the tropics, but the threat to health and tourism in mainland Europe is substantive. Coupled to that are the emerging issues in the European overseas territorities and this paper is the first to consider the impacts in the remoter outposts of Europe. If entomologists and public health authorities are to address the spread of these mosquitoes and mitigate their health risks they must first be prepared to share information to better understand their biology and ecology, and share data on their distribution and control successes. This paper focusses in greater detail on the entomological and ecological aspects of these mosquitoes to assist with the risk assessment process, bringing together a large amount of information gathered through the ECDC VBORNET project.


Asunto(s)
Aedes/fisiología , Insectos Vectores , Especies Introducidas , Adaptación Fisiológica , Aedes/microbiología , Aedes/parasitología , Distribución Animal , Animales , Fiebre Chikungunya/epidemiología , Fiebre Chikungunya/transmisión , Dengue/epidemiología , Dengue/transmisión , Europa (Continente)/epidemiología , Conducta Alimentaria , Fenómenos de Retorno al Lugar Habitual , Control de Mosquitos , Filogeografía , Dinámica Poblacional
8.
Bull Entomol Res ; 105(6): 664-78, 2015 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-26271257

RESUMEN

The distribution of phlebotomine sand flies is widely reported to be changing in Europe. This can be attributed to either the discovery of sand flies in areas where they were previously overlooked (generally following an outbreak of leishmaniasis or other sand fly-related disease) or to true expansion of their range as a result of climatic or environmental changes. Routine surveillance for phlebotomines in Europe is localized, and often one of the challenges for entomologists working in non-leishmaniasis endemic countries is the lack of knowledge on how to conduct, plan and execute sampling for phlebotomines, or how to adapt on-going sampling strategies for other haematophagous diptera. This review brings together published and unpublished expert knowledge on sampling strategies for European phlebotomines of public health concern in order to provide practical advice on: how to conduct surveys; the collection and interpretation of field data; suitable techniques for the preservation of specimens obtained by different sampling methods; molecular techniques used for species identification; and the pathogens associated with sand flies and their detection methods.


Asunto(s)
Insectos Vectores/fisiología , Phlebotomus/fisiología , Animales , Código de Barras del ADN Taxonómico , Europa (Continente) , Insectos Vectores/microbiología , Insectos Vectores/parasitología , Phlebotomus/microbiología , Phlebotomus/parasitología , Densidad de Población , Dinámica Poblacional , Vigilancia de la Población/métodos
9.
Med Vet Entomol ; 28(2): 179-86, 2014 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-23947434

RESUMEN

Two species of arbovirus vector, Culex torrentium and Culex pipiens (Diptera: Culicidae), occur in several European countries, but difficulties in their accurate identification and discrimination have hampered both detailed and large-scale distribution and abundance studies. Using a molecular identification method, we identified to species 2559 larvae of Cx. pipiens/torrentium collected from 138 sites in 13 European countries ranging from Scandinavia to the Mediterranean coast. In addition, samples of 1712 males of Cx. pipiens/torrentium collected at several sites in the Czech Republic were identified to species based on the morphology of their hypopygia. We found that the two species occur together in large areas of Europe, and that Cx. torrentium dominates in northern Europe and Cx. pipiens dominates south of the Alps. The transition in dominance occurs in central Europe, where both species are roughly equally common. There was a strong correlation between the length of the growing season at different sites and occurrences of the two species. As the growing season increases, the proportion and detection of Cx. torrentium decrease, whereas those of Cx. pipiens increase. The present findings have important consequences for the interpretation of the results of studies on major enzootic and link-vectors of mosquito-borne bird-associated viruses (i.e. Sindbis, West Nile and Usutu viruses), especially in central Europe and Scandinavia.


Asunto(s)
Distribución Animal , Culex/fisiología , Insectos Vectores/fisiología , Animales , Arbovirus/fisiología , Culex/anatomía & histología , Culex/genética , Culex/crecimiento & desarrollo , Complejo IV de Transporte de Electrones/genética , Europa (Continente) , Proteínas de Insectos/genética , Larva/genética , Larva/fisiología , Masculino , Proteínas Mitocondriales/genética , Reacción en Cadena de la Polimerasa , Densidad de Población , Estaciones del Año , Especificidad de la Especie
10.
Parasitology ; 140(3): 318-27, 2013 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-23171762

RESUMEN

Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) was evaluated for the rapid identification of ceratopogonid larvae. Optimal sample preparation as evaluated with laboratory-reared biting midges Culicoides nubeculosus was the homogenization of gut-less larvae in 10% formic acid, and analysis of 0.2 mg/ml crude protein homogenate mixed with SA matrix at a ratio of 1:1.5. Using 5 larvae each of 4 ceratopogonid species (C. nubeculosus, C. obsoletus, C. decor, and Dasyhelea sp.) and of 2 culicid species (Aedes aegypti, Ae. japonicus), biomarker mass sets between 27 and 33 masses were determined. In a validation study, 67 larvae belonging to the target species were correctly identified by automated database-based identification (91%) or manual full comparison (9%). Four specimens of non-target species did not yield identification. As anticipated for holometabolous insects, the biomarker mass sets of adults cannot be used for the identification of larvae, and vice versa, because they share only very few similar masses as shown for C. nubeculosus, C. obsoletus, and Ae. japonicus. Thus, protein profiling by MALDI-TOF as a quick, inexpensive and accurate alternative tool is applicable to identify insect larvae of vector species collected in the field.


Asunto(s)
Ceratopogonidae , Culicidae , Insectos Vectores , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos , Animales , Ceratopogonidae/química , Ceratopogonidae/clasificación , Culicidae/química , Culicidae/clasificación , Proteínas de Insectos/química , Insectos Vectores/química , Insectos Vectores/clasificación , Insectos Vectores/parasitología , Larva/química , Larva/clasificación , Parasitología/métodos , Especificidad de la Especie
11.
J Med Entomol ; 49(6): 1226-32, 2012 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-23270149

RESUMEN

Adults of an exotic mosquito, Aedes (Finlaya) koreicus (Edwards) (Diptera: Culicidae) were identified by morphology and genotyping from one site in Belgium in 2008. In late summer of that year, the occurrence of adults and immature stages reconfirmed its presence. This is the first record of this species outside its native range and in particular in Europe. Two subsites of the original location were prospected from April until October 2009 with different traps to evaluate the extent of its presence and establishment in the area and to understand the dynamics of the species' population. Next to Ae. koreicus, 15 other mosquito species were collected. Adult individuals of Ae. koreicus were found from May to September and larvae were still found early October. Larvae were mainly retrieved from artificial containers both in 2008 as in 2009. Containers with eggs and/or larvae were found up to 4 km away from the initial location, indicating the species is spreading locally. Though the introduction route is unknown, it may have occurred via international trade as a large industrial center was located nearby. A comparison of different climatic variables between locations in Belgium with Ae. koreicus and putative source locations in South Korea, revealed similarities between winter temperatures and the number of freezing days and nights in four consecutive years (2004-2008), while humidity and precipitation values differed strongly. The introduction of a new potential disease vector into Europe seems to be a result of proper entrance points, created by intense worldwide trade and suitable environmental conditions.


Asunto(s)
Aedes , Especies Introducidas , Animales , Bélgica , Clima , Femenino , Masculino , Dinámica Poblacional
12.
Euro Surveill ; 17(4)2012 Jan 26.
Artículo en Inglés | MEDLINE | ID: mdl-22297138

RESUMEN

Adult females of two invasive species, Aedes albopictus and Aedes japonicus japonicus, were collected for the first time in July and August 2011 in Germany. Previously, only immature stages of these species had been found in the country. Repeated detection of these species reveals the Upper Rhine Valley in south-west Germany to be a particularly sensitive region for the introduction and establishment of exotic mosquito species that needs careful observation.


Asunto(s)
Aedes/patogenicidad , Control de Mosquitos/métodos , Estaciones del Año , Aedes/genética , Animales , Femenino , Alemania Occidental , Control de Mosquitos/tendencias , Especificidad de la Especie
13.
J Med Entomol ; 48(4): 924-8, 2011 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-21845955

RESUMEN

For the majority of native species, human-created habitats provide a hostile environment that prevents their colonization. However, if the conditions encountered in this novel environment are part of the fundamental niche of a particular species, these low competitive environments may allow strong population expansion of even rare and stenotopic species. If these species are potentially harmful to humans, such anthropogenic habitat alterations may impose strong risks for human health. Here, we report on a recent and severe outbreak of the viciously biting and day-active mosquito Anopheles plumbeus Stephens, 1828, that is caused by a habitat shift toward human-created habitats. Although historic data indicate that the species was previously reported to be rare in Belgium and confined to natural forest habitats, more recent data indicate a strong population expansion all over Belgium and severe nuisance at a local scale. We show that these outbreaks can be explained by a recent larval habitat shift of this species from tree-holes in forests to large manure collecting pits of abandoned and uncleaned pig stables. Further surveys of the colonization and detection of other potential larval breeding places of this mosquito in this artificial environment are of particular importance for human health because the species is known as a experimental vector of West Nile virus and a potential vector of human malaria.


Asunto(s)
Anopheles/fisiología , Insectos Vectores/fisiología , Fiebre del Nilo Occidental/transmisión , Animales , Bélgica , Ecosistema , Actividades Humanas , Humanos , Larva , Crecimiento Demográfico , Fiebre del Nilo Occidental/virología , Virus del Nilo Occidental
14.
Med Vet Entomol ; 25(1): 32-8, 2011 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-21118284

RESUMEN

Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has shown promise in species identification of insect species. We evaluated its potential to consistently characterize laboratory-reared biting midges of the species Culicoides nubeculosus (Meigen) (Diptera: Ceratopogonidae). Twenty-one reproducible potential biomarker masses for C. nubeculosus were identified under different experimental treatments. These treatments included the homogenization of insects in either water or known concentrations of formic acid. The biomarker masses were present independent of age, gender and different periods of storage of individuals in 70% ethanol (a standard preservation method). It was found that the presence of blood in females reduced the intensity of the MALDI-TOF pattern, necessitating the removal of the abdomen before analysis. The protein profiles of a related non-biting midge, Forcipomyia sp. (Diptera: Ceratopogonidae), and of Aedes japonicus japonicus (Theobald) (Diptera: Culicidae) mosquitoes were also examined and were distinctly different. These findings provide preliminary data to optimize future studies in differentiation of species within the Culicoides genus using MALDI-TOF MS which is a rapid, simple, reliable and cost-effective technique.


Asunto(s)
Aedes/clasificación , Ceratopogonidae/clasificación , Proteínas de Insectos/química , Proteómica/métodos , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos , Aedes/química , Envejecimiento , Animales , Biomarcadores/análisis , Biomarcadores/química , Ceratopogonidae/química , Análisis por Conglomerados , Conducta Alimentaria , Femenino , Proteínas de Insectos/análisis , Masculino , Preservación Biológica , Análisis de Componente Principal , Especificidad de la Especie
15.
Euro Surveill ; 15(45)2010 Nov 11.
Artículo en Inglés | MEDLINE | ID: mdl-21087591

RESUMEN

In July 2010, during routine mosquito surveillance inspections at companies that import used tires, three invasive species were found at five locations in the Netherlands: the yellow fever mosquito (Aedes aegypti), the Asian tiger mosquito (Ae. albopictus), and the American rock-pool mosquito (Ae. atropalpus). This is the first time that Ae. aegypti is reported from the Netherlands. Mosquito control was initiated one week after the first invasive mosquito was found, using adulticides and larvicides. The available data suggest that the implemented control measures have been effective for this season.


Asunto(s)
Aedes/clasificación , Comercio , Especies Introducidas , Control de Mosquitos/métodos , Aedes/genética , Animales , Bacillus thuringiensis , Diterpenos , Larva/efectos de los fármacos , Países Bajos , Reacción en Cadena de la Polimerasa , Medición de Riesgo , Estaciones del Año
16.
Science ; 161(3845): 1017-9, 1968 Sep 06.
Artículo en Inglés | MEDLINE | ID: mdl-5671477

RESUMEN

Rats with hypertrophic smooth endoplasmic reticulum (ER) and increased activities of the drug-handling enzymes induced by dieldrin were stressed with larger doses of the pesticide. The activity of the drug-handling enzymes was thus reduced, but liver weight, smooth ER, and P-450 hemoprotein remained elevated. While no changes were apparent by light microscopy, the hypertrophic, hypoactive smooth ER was recognized as tight clusters of tubular membranes associated with abnormalities of the mitochondrial membrane. Similar but not identical morphologic changes were noted in human liver diseases associated with hepatic insufficiency. Hypertrophic, hypoactive smooth ER may indicate transition from adaptation of injury, and can be used as a sensitive parameter of toxicity.


Asunto(s)
Animales , Dieldrín/farmacología , Retículo Endoplásmico , Femenino , Inyecciones Intraperitoneales , Hígado/efectos de los fármacos , Hígado/enzimología , Hígado/patología , Microscopía Electrónica , Mitocondrias Hepáticas , Oxigenasas de Función Mixta/metabolismo , Tamaño de los Órganos , Fosforilación Oxidativa , Oxidorreductasas/metabolismo , Pigmentos Biológicos/análisis , Ratas
17.
J Med Entomol ; 46(6): 1464-7, 2009 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-19960698

RESUMEN

The establishment of the potential vector species Aedes (Finlaya) japonicusjaponicus (Theobald) (Diptera: Culicidae) in southern Belgium is reported. The species was most likely introduced through the international trade in used tires. It was first collected in 2002 on the premises of a second-hand tire company and was sampled using different sampling methods in the two consecutive years (2003-2004). It was only in 2007 and 2008, during a national mosquito survey (MODIRISK), that its presence as adults and larvae at the above-mentioned site and at another tire company in the area was confirmed based on morphological and molecular identification. This discovery is the first record for Belgium of an exotic mosquito species that established successfully and raises the question on the need for monitoring and control. Considering the accompanying species found during the surveys, we also report here the first observation of Culex (Maillotia) hortensis hortensis (Ficalbi) in Belgium.


Asunto(s)
Aedes/fisiología , Aedes/clasificación , Aedes/genética , Animales , Bélgica , Femenino , Masculino , Densidad de Población , Dinámica Poblacional
18.
Med Vet Entomol ; 23(4): 448-51, 2009 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-19941611

RESUMEN

Complaints about a biting pest led to the recognition of invasive Aedes (Finlaya) japonicus japonicus (Theobald) (Diptera: Culicidae) in Central Europe. Larval collections from cemetery vases revealed a colonized area of approximately 1400 km(2) in northern Switzerland spreading into bordering Germany, suggesting that the mosquito has been established in this region for several years. Within this range, larvae of Ae. japonicus were recovered from more containers than the most common resident culicid species Culex pipiens. Possible introduction sites (used tyre yards and international airports) revealed few or no larvae, and the mode of introduction remains unclear. Given the vector potential of this species for arboviruses, implementation of surveillance and control measures should be considered.


Asunto(s)
Aedes/crecimiento & desarrollo , Aedes/genética , Animales , ADN Mitocondrial/química , ADN Mitocondrial/genética , NADH Deshidrogenasa/química , NADH Deshidrogenasa/genética , Polimorfismo de Nucleótido Simple , Análisis de Secuencia de ADN , Suiza
19.
Schweiz Arch Tierheilkd ; 151(5): 205-13, 2009 May.
Artículo en Alemán | MEDLINE | ID: mdl-19421952

RESUMEN

Midges of the genus Culicoides are the only known biological vectors of the bluetongue disease virus (BTV). Their occurrence at altitudes below 900 meters above sea level (m a.s.l.) is monitored in Switzerland by the Swiss Federal Veterinary Office, to determine vector-free periods around winter. In this study, data about the number of midges caught at stations representing the 12 climatic regions of Switzerland are shown. The tiny midges of 1-3 mm in size were caught once per week with UV light traps and grouped under the stereomicroscope into Obsoletus complex, Pulicaris complex and other Culicoides spp. Midges were caught at all stations, albeit in very different numbers. The highest monthly average was 10,000 midges per night (Dittingen/BL); the third highest average of all 12 stations was recorded for the highest-located station (Juf/GR, 2130 m a.s.l.). At stations below 1500 m a.s.l., midges of the Obsoletus complex (98% in Dittingen), which in Central Europe are most likely considered to be responsible for the transmission of BTV, were predominant. With increasing altitude, midges of the Pulicaris complex prevailed (91% in Juf). Hence, there are no regions of the populated areas in Switzerland which are free of midges, but the vector competence regarding BTV of the various midges needs to be urgently clarified.


Asunto(s)
Altitud , Virus de la Lengua Azul/aislamiento & purificación , Lengua Azul/transmisión , Ceratopogonidae/crecimiento & desarrollo , Insectos Vectores/crecimiento & desarrollo , Animales , Lengua Azul/epidemiología , Ceratopogonidae/clasificación , Ceratopogonidae/virología , Clima , Insectos Vectores/clasificación , Insectos Vectores/virología , Filogenia , Estaciones del Año , Suiza/epidemiología , Tiempo (Meteorología)
20.
Schweiz Arch Tierheilkd ; 151(5): 215-21, 2009 May.
Artículo en Alemán | MEDLINE | ID: mdl-19421953

RESUMEN

The aim of this field study was to investigate the occurrence of biting midges (Culicoides spp.), the potential vectors of the bluetongue virus (BTV), in an alpine region of Switzerland (Vals/GR) at altitudes between 1300 and 2000 meters above sea level (m a.s.l.). For this purpose, insects were caught with UV-light traps once weekly from the end of June to the end of October 2008. Midges were found on all altitudes investigated, but distinct differences in the abundance at different stations were noticed. Most midges were caught at the intermediate station (about 1500 m a.s.l.) whereas the catches on the two alps (approximately 2000 m a.s.l.) varied considerably. The predominance of midges belonging to the Pulicaris complex, whose vector competence regarding bluetongue virus (BTV) is largely unknown, rose with increasing altitude. To identify potential breeding habitats, 17 soil samples of three farms were incubated in the laboratory. Different insects emerged but none of them was a Culicoides spp. and, therefore, the habitats of juvenile stages remain unknown. From our results we can conclude that most likely there are no midges-free zones in all of the agriculturally utilized areas (including the alpine summer pastures) of Switzerland. This strongly indicates that cattle, sheep, goats and camelids which are permanently or temporarily kept in regions of higher altitude in Switzerland should be vaccinated against bluetongue.


Asunto(s)
Altitud , Lengua Azul/transmisión , Ceratopogonidae/crecimiento & desarrollo , Insectos Vectores/crecimiento & desarrollo , Animales , Virus de la Lengua Azul/aislamiento & purificación , Ceratopogonidae/clasificación , Ceratopogonidae/virología , Insectos Vectores/clasificación , Insectos Vectores/virología , Prevalencia , Estaciones del Año , Suiza/epidemiología
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA