RESUMEN
Trf4p and Trf5p are non-canonical poly(A) polymerases and are part of the heteromeric protein complexes TRAMP4 and TRAMP5 that promote the degradation of aberrant and short-lived RNA substrates by interacting with the nuclear exosome. To assess the level of functional redundancy between the paralogous Trf4 and Trf5 proteins and to investigate the role of the Trf4-dependent polyadenylation in vivo, we used DNA microarrays to compare gene expression of the wild-type yeast strain of S. cerevisiae with either that of trf4Delta or trf5Delta mutant strains or the trf4Delta mutant expressing the polyadenylation-defective Trf4(DADA) protein. We found little overlap between the sets of transcripts with altered expression in the trf4Delta or the trf5Delta mutants, suggesting that Trf4p and Trf5p target distinct groups of RNAs for degradation. Surprisingly, most RNAs the expression of which was altered by the trf4 deletion were restored to wild-type levels by overexpression of TRF4(DADA), showing that the polyadenylation activity of Trf4p is dispensable in vivo. Apart from previously reported Trf4p and Trf5p target RNAs, this analysis along with in vivo cross-linking and RNA immunopurification-chip experiments revealed that both the TRAMP4 and the TRAMP5 complexes stimulate the degradation of spliced-out introns via a mechanism that is independent of the polyadenylation activity of Trf4p. In addition, we show that disruption of trf4 causes severe shortening of telomeres suggesting that TRF4 functions in the maintenance of telomere length. Finally, our study demonstrates that TRF4, the exosome, and TRF5 participate in antisense RNA-mediated regulation of genes involved in phosphate metabolism. In conclusion, our results suggest that paralogous TRAMP complexes have distinct RNA selectivities with functional implications in RNA surveillance as well as other RNA-related processes. This indicates widespread and integrative functions of TRAMP complexes for the coordination of different gene expression regulatory processes.
Asunto(s)
ADN Polimerasa Dirigida por ADN/metabolismo , ARN Polimerasas Dirigidas por ADN/metabolismo , ARN/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/metabolismo , ADN Polimerasa Dirigida por ADN/genética , ARN Polimerasas Dirigidas por ADN/genética , Exosomas/metabolismo , Regulación Fúngica de la Expresión Génica/fisiología , Intrones/genética , Mutación , Poliadenilación , Interferencia de ARN , Estabilidad del ARN , ARN sin Sentido/metabolismo , ARN no Traducido/metabolismo , Proteínas Ribosómicas/genética , Proteínas Ribosómicas/metabolismo , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/genética , Telómero/metabolismoRESUMEN
We describe ribosome affinity purification (RAP), a method that allows rapid purification of ribosomes and associated messages from the yeast Saccharomyces cerevisiae. The method relies on the expression of protein A tagged versions of the ribosomal protein Rpl16, which is used to efficiently recover endogenously formed ribosomes and polysomes from cellular extracts with IgG-coupled spherical microbeads. This approach can be applied to profile reactions of the translatome, which refers to all messages associated with ribosomes, with those of the transcriptome using DNA microarrays. In addition, ribosomal proteins, their modifications, and/or other associated proteins can be mapped with mass spectrometry. Finally, application of this method in other organisms provides a valuable tool to decipher cell-type specific gene expression patterns.
Asunto(s)
Cromatografía de Afinidad/métodos , Proteínas Ribosómicas/aislamiento & purificación , Proteínas de Saccharomyces cerevisiae/aislamiento & purificación , Fraccionamiento Celular/métodos , Análisis por Matrices de Proteínas/métodos , Proteínas de Unión al ARN/aislamiento & purificación , Ribosomas/química , Saccharomyces cerevisiae/genéticaRESUMEN
Caseous lymphadenitis (CLA), a disease affecting sheep and goats, is caused by Corynebacterium pseudotuberculosis (Cp). Eradication programs are based on the serological identification of Cp infected animals. However, available diagnostic ELISAs are not similarly suitable for sheep and goats. In the present study the comparison of antigens revealed major species specific differences between sheep and goat derived Cp field isolates as well as between field isolates and the Cp ATCC reference strains. Furthermore, we found species-specific differences in the anti-Cp humoral immune response between sheep and goats. The analysis of band frequency was able to distinguish between immunodominant and non-immunodominant protein bands. The 150 kDa, 74 kDa, 48 kDa, and 30 kDa antigens were immunodominant in both, sheep and goats. Interestingly, the most commonly used diagnostic antigen, i.e. the 30 kDa phospholipase D (PLD), was recognized by 100% of the Cp positive goats but only by 70% of the Cp positive sheep. Furthermore, analysis of field sera revealed that there were a particular percentage of Cp positive sera which reacted negative with the PLD. In conclusion our results clearly showed that (1) the application of a combination of further defined immunodominant Cp antigens - in addition to the PLD antigen - and (2) consideration of species-specific differences in the anti-Cp immune response will substantially contribute to the improvement of Cp serological diagnostics and to effective eradication programs in both sheep and goats.
Asunto(s)
Antígenos Bacterianos/química , Infecciones por Corynebacterium/veterinaria , Corynebacterium pseudotuberculosis/inmunología , Enfermedades de las Cabras/microbiología , Inmunidad Humoral/inmunología , Enfermedades de las Ovejas/microbiología , Animales , Infecciones por Corynebacterium/diagnóstico , Infecciones por Corynebacterium/inmunología , Infecciones por Corynebacterium/microbiología , Ensayo de Inmunoadsorción Enzimática/veterinaria , Enfermedades de las Cabras/diagnóstico , Enfermedades de las Cabras/inmunología , Cabras , Fosfolipasa D/química , Fosfolipasa D/inmunología , Fosfolipasa D/metabolismo , Reproducibilidad de los Resultados , Ovinos , Enfermedades de las Ovejas/inmunologíaRESUMEN
BACKGROUND: Glucose inhibition of gluconeogenic growth suppressor 2 protein (Gis2p) and zinc-finger protein 9 (ZNF9) are conserved yeast and human zinc-finger proteins. The function of yeast Gis2p is unknown, but human ZNF9 has been reported to bind nucleic acids, and mutations in the ZNF9 gene cause the neuromuscular disease myotonic dystrophy type 2. To explore the impact of these proteins on RNA regulation, we undertook a systematic analysis of the RNA targets and of the global implications for gene expression. RESULTS: Hundreds of mRNAs were associated with Gis2p, mainly coding for RNA processing factors, chromatin modifiers and GTPases. Target mRNAs contained stretches of G(A/U)(A/U) trinucleotide repeats located in coding sequences, which are sufficient for binding to both Gis2p and ZNF9, thus implying strong structural conservation. Predicted ZNF9 targets belong to the same functional categories as seen in yeast, indicating functional conservation, which is further supported by complementation of the large cell-size phenotype of gis2 mutants with ZNF9. We further applied a matched-sample proteome-transcriptome analysis suggesting that Gis2p differentially coordinates expression of RNA regulons, primarily by reducing mRNA and protein levels of genes required for ribosome assembly and by selectively up-regulating protein levels of myosins. CONCLUSIONS: This integrated systematic exploration of RNA targets for homologous RNA-binding proteins indicates an unexpectedly high conservation of the RNA-binding properties and of potential targets, thus predicting conserved RNA regulons. We also predict regulation of muscle-specific genes by ZNF9, adding a potential link to the myotonic dystrophy related phenotypes seen in ZNF9 mouse models.
Asunto(s)
ARN Mensajero/metabolismo , Proteínas de Unión al ARN/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Secuencia de Aminoácidos , Proliferación Celular , Tamaño de la Célula , Codón Iniciador , Secuencia Conservada , Perfilación de la Expresión Génica , Regulación de la Expresión Génica , Humanos , Datos de Secuencia Molecular , Sistemas de Lectura Abierta/genética , Fenotipo , Proteoma , ARN Mensajero/química , Regulón , Secuencias Repetitivas de Ácidos Nucleicos , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Transcriptoma , Dedos de ZincRESUMEN
RNA-binding proteins (RBPs) play important roles in the posttranscriptional control of gene expression. However, our understanding of how RBPs interact with each other at different regulatory levels to coordinate the RNA metabolism of the cell is rather limited. Here, we construct the posttranscriptional regulatory network among 69 experimentally studied RBPs in yeast to show that more than one-third of the RBPs autoregulate their expression at the posttranscriptional level and demonstrate that autoregulatory RBPs show reduced protein noise with a tendency to encode for hubs in this network. We note that in- and outdegrees in the posttranscriptional RBP-RBP regulatory network exhibit gaussian and scale-free distributions, respectively. This network was also densely interconnected with extensive cross-talk between RBPs belonging to different posttranscriptional steps, regulating varying numbers of cellular RNA targets. We show that feed-forward loops and superposed feed-forward/feedback loops are the most significant three-node subgraphs in this network. Analysis of the corresponding protein-protein interaction (posttranslational) network revealed that it is more modular than the posttranscriptional regulatory network. There is significant overlap between the regulatory and protein-protein interaction networks, with RBPs that potentially control each other at the posttranscriptional level tending to physically interact and being part of the same ribonucleoprotein (RNP) complex. Our observations put forward a model wherein RBPs could be classified into those that can stably interact with a limited number of protein partners, forming stable RNP complexes, and others that form transient hubs, having the ability to interact with multiple RBPs forming many RNPs in the cell.
Asunto(s)
Procesamiento Proteico-Postraduccional , Procesamiento Postranscripcional del ARN , Proteínas de Unión al ARN/metabolismo , Modelos BiológicosRESUMEN
Hundreds of RNA-binding proteins (RBPs) control diverse aspects of post-transcriptional gene regulation. To identify novel and unconventional RBPs, we probed high-density protein microarrays with fluorescently labeled RNA and selected 200 proteins that reproducibly interacted with different types of RNA from budding yeast Saccharomyces cerevisiae. Surprisingly, more than half of these proteins represent previously known enzymes, many of them acting in metabolism, providing opportunities to directly connect intermediary metabolism with posttranscriptional gene regulation. We mapped the RNA targets for 13 proteins identified in this screen and found that they were associated with distinct groups of mRNAs, some of them coding for functionally related proteins. We also found that overexpression of the enzyme Map1 negatively affects the expression of experimentally defined mRNA targets. Our results suggest that many proteins may associate with mRNAs and possibly control their fates, providing dense connections between different layers of cellular regulation.
Asunto(s)
Aminopeptidasas/metabolismo , ARN Mensajero/metabolismo , Proteínas de Unión al ARN/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/metabolismo , Aminopeptidasas/genética , Enzimas/genética , Enzimas/metabolismo , Perfilación de la Expresión Génica , Metionil Aminopeptidasas , Análisis de Secuencia por Matrices de Oligonucleótidos , Análisis por Matrices de Proteínas , Unión Proteica , Proteoma/genética , Proteoma/metabolismo , Proteómica , ARN de Hongos/genética , ARN de Hongos/metabolismo , ARN Mensajero/genética , Proteínas de Unión al ARN/genética , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/genéticaRESUMEN
mRNAs encoding mitochondrial proteins are enriched in the vicinity of mitochondria, presumably to facilitate protein transport. A possible mechanism for enrichment may involve interaction of the translocase of the mitochondrial outer membrane (TOM) complex with the precursor protein while it is translated, thereby leading to association of polysomal mRNAs with mitochondria. To test this hypothesis, we isolated mitochondrial fractions from yeast cells lacking the major import receptor, Tom20, and compared their mRNA repertoire to that of wild-type cells by DNA microarrays. Most mRNAs encoding mitochondrial proteins were less associated with mitochondria, yet the extent of decrease varied among genes. Analysis of several mRNAs revealed that optimal association of Tom20 target mRNAs requires both translating ribosomes and features within the encoded mitochondrial targeting signal. Recently, Puf3p was implicated in the association of mRNAs with mitochondria through interaction with untranslated regions. We therefore constructed a tom20 Delta puf3 Delta double-knockout strain, which demonstrated growth defects under conditions where fully functional mitochondria are required. Mislocalization effects for few tested mRNAs appeared stronger in the double knockout than in the tom20 Delta strain. Taken together, our data reveal a large-scale mRNA association mode that involves interaction of Tom20p with the translated mitochondrial targeting sequence and may be assisted by Puf3p.