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Due to increasing public concern over hygiene, there have been many studies investigating antimicrobial and antiviral agents recently. With the aim of developing biobased virucidal/virus capture agents, we report a chemical modification of the cellulose nanocrystals (CNCs) surface with poly(2-dimethylamino) ethyl acrylate) methyl chloride quaternary salt (Q-PDMAEA) to introduce the positively charged functional groups. The surface of CNCs was modified through direct and indirect graft polymerization. Subsequently, the direct and indirect cationization effect on the degree of functionalization, thermal stability, crystallinity, and antiviral activity of CNCs was investigated. Indirect cationization produced the highest degree of polymer grafting, increasing particle size and thermal stability. Further, the modified CNCs were tested for their ability to capture nonenveloped bacteriophages PhiX174 (ΦX174) and MS2. We observed a significant (>4.19 log10) reduction in total viral load by specific functionalized CNCs. However, the activity depended on the structure of functional groups, surface charge density, and the type of virus under study. Overall, the direct and indirect cationization of CNC leads to biobased agents with immobilized cationic charge, with good virus capture activity. Such agents can be used for various applications including textiles, packaging, wastewater treatment, etc.
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Traditional metal-alloy bone fixation devices provide structural support for bone repair but have limitations in actively promoting bone healing and often require additional surgeries for implant removal. In this study, we focused on addressing these challenges by fabricating biodegradable composites using poly(lactic acid) (PLA) and strontium-substituted nanohydroxyapatite (SrHAP) via melt compounding and injection molding. Various percentages of SrHAP (5, 10, 20, and 30% w/w) were incorporated into the PLA matrix. We systematically investigated the structural, morphological, thermal, mechanical, rheological, and dynamic mechanical properties of the prepared composites. Notably, the tensile modulus, a critical parameter for orthopedic implants, significantly improved from 2.77 GPa in pristine PLA to 3.73 GPa in the composite containing 10% w/w SrHAP. The incorporation of SrHAP (10% w/w) into the PLA matrix led to an increased storage modulus, indicating a uniform dispersion of SrHAP within the PLA and good compatibility between the polymer and nanoparticles. Moreover, we successfully fabricated screws using PLA composites with 10% (w/w) SrHAP, demonstrating their formability at room temperature and radiopacity when observed under X-ray microtomography (micro-CT). Furthermore, the water contact angle decreased from 93 ± 2° for pristine PLA to 75 ± 3° for the composite containing SrHAP, indicating better surface wettability. To assess the biological behavior of the composites, we conducted in vitro cell-material tests, which confirmed their osteoconductive and osteoinductive properties. These findings highlight the potential of our developed PLA/SrHAP10 (10% w/w) composites as machinable implant materials for orthopedic applications. In conclusion, our study presents the fabrication and comprehensive characterization of biodegradable composites comprising PLA and strontium-substituted nanohydroxyapatite (SrHAP). These composites exhibit improved mechanical properties, formability, and radiopacity while also demonstrating desirable biological behavior. Our results suggest that these PLA/SrHAP10 composites hold promise as machinable implant materials for orthopedic applications.
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Poliésteres , Estroncio , Poliésteres/química , Polímeros/química , Prótesis e ImplantesRESUMEN
Graphene family nanomaterials (GFNs) are rapidly emerging for ocular applications due to their outstanding physicochemical properties. Since the eyes are very sensitive organs and the contact between the eyes and GFNs in eye drops, contact lenses, intraocular drug delivery systems and biosensors and even the workers handling these nanomaterials is inevitable, it is necessary to investigate their ocular toxicities and physiological interactions with cells as well as their toxicity mechanisms. The toxicity of GFNs can be extremely affected by their physicochemical properties, including composition, size, surface chemistry, and oxidation level as well as dose and the time of exposure. Up to now, there are several studies on the in vitro and in vivo toxicity of GFNs; however, a comprehensive review on ocular toxicity and applications of GFNs is missing, and a knowledge about the health risks of eye exposure to the GFNs is predominantly unspecified. This review highlights the ocular applications of GFNs and systematically covers the most recent advances of GFNs' physicochemical properties, in vitro and in vivo ocular toxicity, and the possible toxicity mechanisms as well as provides some perspectives on the potential risks of GFNs in material development and biomedical applications.
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Ojo/efectos de los fármacos , Grafito/efectos adversos , Nanoestructuras/efectos adversos , Soluciones Oftálmicas/efectos adversos , Grafito/química , Humanos , Nanoestructuras/química , Soluciones Oftálmicas/químicaRESUMEN
Implants of bioresorbable materials combined with osteoconductive calcium phosphate ceramics show promising results to replace and repair damaged bone tissue. Here we present additive manufacturing of patient-specific porous scaffolds of poly(trimethylene carbonate) (PTMC) including high amounts of ß-tricalcium phosphate (ß-TCP). Tensile testing of composite networks showed that addition of ß-tricalcium phosphate reinforces the composites significantly. Three-dimensional structures containing up to 60 wt % ß-TCP could be built by stereolithography. By lowering the content to 51 wt %, manufacturing of a large-sized patient-specific prototype was possible at high resolution. Closer examination revealed that the created scaffolds contained more ß-TCP on the surface of the builds. Stereolithography therefore provides a manufacturing technique where the bioactive agent is directly available for creating an enhanced microenvironment for cell growth. The biocompatibility and bioresorption of PTMC coupled with the osteoconductivity of ß-TCP are an important candidate to consider in additive manufacturing of bone regeneration implants.
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Regeneración Ósea , Fosfatos de Calcio/química , Dioxanos/química , Polímeros/química , Estereolitografía , Andamios del Tejido/química , Rastreo Diferencial de Calorimetría , Ensayo de Materiales , Polimerizacion , Porosidad , Resistencia a la Tracción , TermogravimetríaRESUMEN
Despite their sustainable appeal, biomass components are currently undervalued in nanotechnology because means to control the assembly of bio-based nanoparticles are lagging behind the synthetic counterparts. Here, micrometer-sized particles consisting of aligned cellulose nanocrystals (CNCs) are prepared by crosslinking cellulose in cotton linter fibers that are prehydrolyzed with gaseous HCl, resulting in chemical cleavage necessary for CNC formation but retaining the morphology of the native fibers. That way, the intrinsic alignment of cellulose microfibrils within the fiber cell wall can be retained and utilized for top-down CNC alignment. Subsequent crosslinking with citric acid cements the alignment and preserves it, following the dispersion of CNCs trapped end-to-end, connected, and crosslinked within the colloidally stable micrometer-sized particles. Furthermore, thermoporosimetry and cryogenic transmission electron microscopy (Cryo TEM) shows that the particles possess mainly nanoporous (<2 nm) character in water. The approach challenges the current paradigm of predominantly bottom-up methods for nanoparticle assembly.
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Pared Celular/química , Celulosa/química , Nanopartículas/química , Hidrólisis , Microscopía de Fuerza Atómica , Microscopía Electrónica de Transmisión , Nanopartículas/ultraestructura , Nanoporos/ultraestructura , Nanotecnología , Tamaño de la Partícula , Propiedades de Superficie , Agua/químicaRESUMEN
Recent trends in peripheral nerve regeneration are directed toward the development of nerve guidance channels to assist the regeneration of the nerves across critical size defects. Advanced nerve guidance channels (aNGCs) should possess multifunctional properties to direct the axonal regeneration from proximal to distal end, allow the concentration of growth factors secreted by the injured nerve end, and attenuate the ingrowth of scar tissue at the site of injury. The design of the nerve guidance channel (NGC) is critical for providing the necessary topographical, chemotactic, as well as haptotactic cues for efficient nerve regeneration. In this study, we have designed and fabricated clinically relevant aNGCs comprising an antioxidant polyurethane (PUAO) conduit filled with aligned chitosan-gelatin (CG) cryogel filler for peripheral nerve regeneration. The effects of temperature, polymer concentration, and cross-linker concentration on the physicochemical properties of the CG cryogel filler were studied. The synthesized scaffolds were evaluated by scanning electron microscopy (SEM) and compression testing to obtain the matrix best suited to form the aNGC. The nanofibrous PUAO conduit was fabricated by electrospinning with a wall thickness of 114.16 ± 26.91 µm, which was filled with CG (1.2/6.4%)-aligned cryogel matrix to obtain the aNGCs. The aNGCs with 2.01 ± 0.04 mm internal diameter, 15 mm length, and internal CG filler with a pore diameter of 29.60 ± 9.83 µm were fabricated. The aNGCs were evaluated by SEM and in vitro neuronal culture for biocompatibility and cellular alignment. In vitro dorsal root ganglion cultures showed the aligned growth and cellular migration along the aligned pores of aNGCs. With this study, we conclude that this clinically relevant aligned porous aNGC will have a promising effect in repair and regeneration of peripheral nerve injuries.
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Quitosano/análogos & derivados , Criogeles/química , Gelatina/química , Regeneración Nerviosa , Poliuretanos/química , Ingeniería de Tejidos/métodos , Animales , Línea Celular Tumoral , Células Cultivadas , Ratones , Nanocompuestos/química , Ratas , Andamios del Tejido/químicaRESUMEN
A wood based yarn platform for capturing pharmaceutical molecules from water was developed. Cellulose fiber yarns were modified with cyclodextrins, and the capture of 17α-ethinyl estradiol (EE2), a synthetic estrogen hormone used as contraceptive, from water was tested. The yarns were prepared by spinning a deep eutectic solution (DES) of cellulose in choline chloride-urea. Despite their high porosity and water sorption capacity (5 g/g), the spun fiber yarns displayed high wet strength, up to 60% of that recorded in dry condition (128 MPa with 17% strain at break). Cyclodextrin irreversible attachment on the yarns was achieved with adsorbed chitosan and the conjugation reactions and capture of EE2 by the cyclodextrin-modified cellulose were confirmed via online detection with Surface Plasmon Resonance (SPR). The facile synthesis of the bioactive yarns and EE2 binding capacity from aqueous matrices (as high as 2.5 mg/g) indicate excellent prospects for inexpensive platforms in disposable affinity filtration. The study presents a strategy to produce a wood fiber based yarn to be used as a platform for human and veterinary pharmaceutical hormone capture.
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Etinilestradiol/química , Resonancia por Plasmón de Superficie , Madera/química , beta-Ciclodextrinas/química , Animales , HumanosRESUMEN
Wheat arabinoxylan was treated with two α-arabinofuranosidases exhibiting different mode of action to create three different polymeric substrates. These three substrate preparations were characterized by xylopyranose backbone sugars that are (1) singly substituted by arabinose at C2 or C3, (2) doubly substituted by arabinose at C2 and C3, and (3) largely unsubstituted. All xylan preparations were grafted with glycidyl methacrylate using cerium ammonium nitrate and then evaluated in terms of graft yield and adsorption to cellulose surfaces. The highest graft yield was observed for the xylan preparation characterized by a largely unsubstituted xylopyranose backbone. Furthermore, QCM-D analyses revealed that grafted xylans exhibited a two-stage desorption pattern, which was not seen with the ungrafted xylans and was consistent with increased water sorption. Accordingly, this study demonstrates the potential of arabinofuranosidases to increase the yield and influence the viscoelastic properties of grafted xylans used as biobased cellulose coatings.
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Celulosa/análogos & derivados , Glicósido Hidrolasas/metabolismo , Xilanos/química , Adsorción , Biocatálisis , Elasticidad , Polimerizacion , Viscosidad , Xilosa/análogos & derivados , Xilosa/químicaRESUMEN
With the fluorescent stain Nile Red (NR), phytoplankton lipid accumulation can be monitored quickly and in situ. In the light of recent results in phytoplankton diversity research, there is also a need for cell- and species-specific lipid measurement techniques. The objective of this work was to investigate whether cell-specific phytoplankton lipid accumulation could be monitored with the image-based particle analyzer FlowCAM™ and NR staining. Applying Phaeodactylum tricornutum as a model species, we compared the FlowCAM method to two established lipid quantification methods: spectrofluorometric NR fluorescence measurement and total lipid analysis by gas chromatography. The experiment was carried out in batch cultures under nitrogen limitation to induce lipid accumulation. We showed significant correlation between the three different lipid quantification methods confirming the applicability of the novel FlowCAM method in cell-specific and near real-time lipid quantification. Furthermore, with the method described here, the lipid content of taxonomically distinguished cells can eventually be measured from multispecies cultures, opening several new possibilities to study species-specific responses to stress conditions and the complementarity effect.
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Diatomeas/metabolismo , Espectrometría de Fluorescencia/métodos , Bioensayo/métodos , Metabolismo de los Lípidos/fisiología , Oxazinas/metabolismoRESUMEN
Transparent and flexible energy storage devices have garnered great interest due to their suitability for display, sensor and photovoltaic applications. In this paper, we report the application of aerosol synthesized and dry deposited single-walled carbon nanotube (SWCNT) thin films as electrodes for an electrochemical double-layer capacitor (EDLC). SWCNT films exhibit extremely large specific capacitance (178 F g(-1) or 552 µF cm(-2)), high optical transparency (92%) and stability for 10 000 charge/discharge cycles. A transparent and flexible EDLC prototype is constructed with a polyethylene casing and a gel electrolyte.
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Thiol-ene click reaction was successfully employed for chemical modification of graphene oxide (GO) by one-step synthesis. Herein, 2,2-azobis(2-methylpropionitrile) (AIBN) was used as thermal catalyst and cysteamine hydrochloride (HS-(CH2 )2 -NH2 HCl) was used as thiol-containing compound, which is incorporated to GO surface upon reaction with the C=C bonds. The hydrochloride acts as protecting group for the amine, which is finally eliminated by adding sodium hydroxide. The modified GO contains both S- and N-containing groups (NS-GO). We found that NS-GO sheets form good dispersion in water, ethanol, and ethylene glycol. These graphene dispersions can be processed into functionalized graphene film. Besides, it was demonstrated that NS-GO was proved to be an excellent host matrix for platinum nanoparticles. The developed method paves a new way for graphene modification and its functional nanocomposites.
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Compounds that can gelate aqueous solutions offer an intriguing toolbox to create functional hydrogel materials for biomedical applications. Amphiphilic Janus dendrimers with low molecular weights can readily form self-assembled fibers at very low mass proportion (0.2â wt %) to create supramolecular hydrogels (G'â«G'') with outstanding mechanical properties and storage modulus of G'>1000â Pa. The G' value and gel melting temperature can be tuned by modulating the position or number of hydrophobic alkyl chains in the dendrimer structure; thus enabling exquisite control over the mesoscale material properties in these molecular assemblies. The gels are formed within seconds by simple injection of ethanol-solvated dendrimers into an aqueous solution. Cryogenic TEM, small-angle X-ray scattering, and SEM were used to confirm the fibrous structure morphology of the gels. Furthermore, the gels can be efficiently loaded with different bioactive cargo, such as active enzymes, peptides, or small-molecule drugs, to be used for sustained release in drug delivery.
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Dendrímeros/química , Hidrogeles/química , Péptidos/química , Tensoactivos/química , Sistemas de Liberación de Medicamentos , Liberación de Fármacos , Etanol/química , Interacciones Hidrofóbicas e Hidrofílicas , TemperaturaAsunto(s)
Materiales Biocompatibles/química , Polímeros/química , Bioimpresión/métodos , Dioxanos/química , Sistemas de Liberación de Medicamentos , Electrónica/instrumentación , Hidrogeles/química , Ácidos Nucleicos/administración & dosificación , Impresión Tridimensional , Ingeniería de Tejidos/métodosRESUMEN
The grafting reactivities with glycidyl methacrylate (GMA) of five xylans from hardwood and cereal sources were compared. The structural property that best predicted the reactivities of xylans with GMA was the fraction of 4-O-methylglucuronic acid (MeGlcA) substitution. A comparatively high level of arabinose substitution was also positively correlated to reactivity with GMA. The impact of MeGlcA and arabinose branching groups is likely attributed to the solubilizing effect of these substituents. Consistent with this prediction, low water solubility and high lignin content were found to hinder reactivity. Even though oligomeric substrates have the advantage of water solubility, modified xylo-oligosaccharides were difficult to purify. Accordingly, delignified and high-molecular weight xylans that are soluble or dispersible in water are best suited for this type of backbone derivatization. Adsorption studies with a quartz crystal microbalance with dissipation indicated that grafting lowered the total adsorption of arabinoxylan but did not significantly affect the fraction of xylans adsorbed irreversibly on cellulose.
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Celulosa/química , Polimerizacion , Xilanos/química , Arabinosa/química , Compuestos Epoxi/química , Glucuronatos/química , Metacrilatos/químicaRESUMEN
We present two facile approaches for introducing multivalent displays of tyrosine sulfate mimetic ligands on the surface of cellulose nanocrystals (CNCs) for application as viral inhibitors. We tested the efficacy of cellulose nanocrystals, prepared either from cotton fibers or Whatman filter paper, to inhibit alphavirus infectivity in Vero (B) cells. Cellulose nanocrystals were produced by sulfuric acid hydrolysis leading to nanocrystal surfaces decorated with anionic sulfate groups. When the fluorescent marker expressing Semliki Forest virus vector, VA7-EGFP, was incubated with CNCs, strong inhibition of virus infectivity was achieved, up to 100 and 88% for cotton and Whatman CNCs, respectively. When surface sulfate groups of CNCs were exchanged for tyrosine sulfate mimetic groups (i.e. phenyl sulfonates), improved viral inhibition was attained. Our observations suggest that the conjugation of target-specific functionalities to CNC surfaces provides a means to control their antiviral activity. Multivalent CNCs did not cause observable in vitro cytotoxicity to Vero (B) cells or human corneal epithelial (HCE-T) cells, even within the 100% virus-inhibitory concentrations. Based on the similar chemistry of known polyanionic inhibitors, our results suggest the potential application of CNCs as inhibitors of other viruses, such as human immunodeficiency virus (HIV) and herpes simplex viruses.
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Infecciones por Alphavirus/tratamiento farmacológico , Antivirales/química , Antivirales/farmacología , Celulosa/síntesis química , Nanopartículas/química , Nanotecnología/métodos , Virus de los Bosques Semliki/efectos de los fármacos , Tirosina/análogos & derivados , Animales , Biomimética , Celulosa/química , Chlorocebus aethiops , Epitelio Corneal/citología , Epitelio Corneal/efectos de los fármacos , Genes erbB-1 , Ligandos , Nanopartículas/toxicidad , Virus de los Bosques Semliki/genética , Espectroscopía Infrarroja por Transformada de Fourier , Tirosina/química , Células Vero/efectos de los fármacos , Células Vero/virologíaRESUMEN
OBJECTIVES: To characterize biological response to subcutaneously implanted macroporous poly(ε-caprolactone/D,L-lactide)-based scaffolds, and to evaluate the effect of bioactive glass (BAG) filler and osteogenic cells to the tissue response and ectopic bone formation. MATERIAL AND METHODS: In the first part of this study, six different scaffold types were screened in a rat subcutaneous implantation model. The polymer scaffolds with 70/30 caprolactone/lactide ratio and corresponding composites with < 45 µm BAG filler size were chosen for the further ectopic bone formation assay. The scaffolds were loaded with differentiating bone marrow stromal cells and implanted subcutaneously in syngeneic rats. RESULTS: With plain scaffolds, only mild foreign body reaction with no signs of gross inflammation was observed after 4 weeks of implantation. Furthermore, the scaffolds were fully invaded by well-vascularized soft connective tissue. Overall, all the tested scaffold types showed an appropriate host response. With cell-seeded scaffolds, several loci of immature mineralizing tissue and small amounts of mature bone were observed after 4 weeks. The incidence of mature bone formation was two and four in polymer scaffolds and composites, respectively (n = 8). After twelve weeks, mature bone was observed in only one polymer scaffold but in seven composites (n = 8). Excluding bone formation, the host response was considered similar to that with cell-free scaffolds. CONCLUSIONS: Plain scaffolds supported the ingrowth of well-vascularized fibroconnective tissue. Furthermore, cell seeded composites with BAG filler showed enhanced ectopic bone formation in comparison with corresponding neat polymer scaffolds.
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Huesos , Coristoma/patología , Reacción a Cuerpo Extraño/patología , Vidrio/química , Poliésteres/química , Enfermedades de la Piel/patología , Andamios del Tejido/química , Animales , Materiales Biocompatibles , Biopsia , Sustitutos de Huesos , Ensayo de Materiales , Porosidad , Ratas , Ratas Endogámicas F344 , Ratas Sprague-DawleyRESUMEN
To understand the enzymatic degradation behavior of crosslinked polylactide (PLA), the preparation and enzymatic degradation of both thermoplastic (linear) and crosslinked PLAs that have pore structures with different dimensions were carried out. The porous structures of the linear PLA samples were of micro and nanoporous nature, and prepared by batch foaming with supercritical CO2 and compared with the porous structures of crosslinked PLA (Lait-X) created by the salt leaching method. The surface and cross-sectional morphologies of the porous structures were investigated by using scanning electron microscopy. The morphological analysis of porous Lait-X showed a rapid loss of physical features within 120 h of exposure to proteinase-K enzymatic degradation at 37 °C. Due to the higher affinity for water, enhanced enzymatic activity as compared to the linear PLA porous structures in the micro and nanoporous range was observed.
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Materiales Biocompatibles/metabolismo , Endopeptidasa K/metabolismo , Poliésteres/metabolismo , Materiales Biocompatibles/química , Poliésteres/química , Porosidad , Espectroscopía Infrarroja por Transformada de Fourier , Difracción de Rayos XRESUMEN
The demand for new biocompatible and 3D printable materials for biomedical applications is on the rise. Ideally, such materials should exhibit either biodegradability or recyclability, possess antibacterial properties, and demonstrate remarkable biocompatibility with no cytotoxic effects. In this research, we synthesized biocompatible and 3D printable hydrogels tailored for biomedical applications, such as wound healing films, by combining antibacterial double-quaternized chitosan (DQC) with cystamine-based non-isocyanate polyurethane (NIPU-Cys) - a material renowned for enhancing both the flexibility and mechanical properties of the hydrogels. To improve the rheological behavior, swelling attributes, and printability, cellulose nanofibrils were introduced into the matrix. We investigated the impact of DQC on degradability, swelling capacity, rheological behavior, printability, and cell biocompatibility. The slightly cytotoxic nature associated with quaternary chitosan was evaluated, and the optimal concentration of DQC in the hydrogel was determined to ensure biocompatibility. The resulting hydrogels were found to be suitable materials for 3D printing via a direct ink writing technique (DIW), producing porous, biocompatible hydrogels endowed with valuable attributes suitable for various wound-healing applications.
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Quitosano , Nanofibras , Poliuretanos , Isocianatos , Hidrogeles/farmacología , Celulosa/farmacología , Tinta , Materiales Biocompatibles/farmacología , Cicatrización de Heridas , Antibacterianos , Impresión TridimensionalRESUMEN
Three-armed poly(trimethylene carbonate) (PTMC) and poly(trimethylene carbonate-co-Æ-caprolactone) (P(TMC-co-ε-CL)) macromers with molecular weights of approximately 30 kg mol-1 are synthesized by ring-opening polymerization and subsequent functionalization with methacrylic anhydride. Networks are then prepared by photo-crosslinking. To investigate the in vitro and in vivo degradation properties of these photo-crosslinked networks and assess the effect of ε-caprolactone content on the degradation properties, PTMC networks, and copolymer networks with two different TMC:ε-CL ratios are prepared. PTMC networks degraded slowly, via an enzymatic surface erosion process, both in vitro and in vivo. Networks prepared from P(TMC-co-ε-CL) macromers with a 74:26 ratio are found to degrade slowly as well, via a surface erosion process, albeit at a higher rate compared to PTMC networks. Increasing the ε-CL content to a ratio of 52:48, resulted in a faster degradation. These networks lost their mechanical properties much sooner than the other networks. Thus, PTMC and P(TMC-co-ε-CL) networks are interesting networks for tissue engineering purposes and the exact degradation properties can be tuned by varying the TMC:ε-CL ratio, providing researchers with a tool to obtain copolymer networks with the desired degradation rate depending on the intended application.
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Caproatos , Lactonas , Poliésteres , Polímeros , Polímeros/metabolismo , DioxanosRESUMEN
3D printed scaffolds have revolutionized the field of regenerative medicine by overcoming the lacunas such as precision, customization, and reproducibility observed through traditional methods of scaffold preparation such as freeze-drying, electrospinning, etc. Combining the advantages of 3D printed scaffolds along with bioactive cues such as signaling molecules can be an effective treatment approach. In the present study, cellulose nanocrystals (CNCs) along with gelatin, in different ratios, were used for scaffold preparation through the direct ink writing technique and thoroughly characterized. The scaffolds showed porous microstructure, high swelling ratio (â¼390 to 590), degradability and porosity (â¼65 %). In vitro biocompatibility assays showed high biocompatibility and no toxicity through live-dead, proliferation and hemolysis assay. Further, the optimum formulation was functionalized with nitric oxide (NO)-releasing modified gelatin to enhance the scaffold's biomedical applicability. Functionality assays with this formulation, scratch, and neurite outgrowth showed positive effects of NO on cell migration and neurite length. The study presents the fabrication, modification, and biomedical applicability of the aforementioned inks, which paves new pathways in the field of 3D printing of scaffolds with significant potential for biomedical applications, soft tissue engineering, and wound dressing, for example.