RESUMEN
Cells derived from the fetal liver have been shown to be a rich source of progenitor stem cells, constituting a promising source for Tissue Engineering and Regenerative Medicine. In this study, embryo and fetal liver-bud derived cells from Fischer 344 rats were obtained at E12.5, E14.5 and E16.5 gestational days and evaluated for cell phenotype, survival and proliferation. Liver transaminase (AST and ALT) and AFP levels were lower in embryo liver-bud-derived cells on day 12.5. Markers for stem cells, cell cycle progression and cell death were differentially expressed in E12.5 cell cultures. Analysis of mitochondrial electric potential on 14.5 and 16.5 days showed a tendency for cells with lower functional or metabolic ability, in comparison to cultures derived from day 12.5. The results demonstrated that the majority of the E16.5 cells were in the G0 / G1 phase. The capacity of synthesis (S) and cellular division (G2 / M) of embryo and fetal liver bud-derived cells was constant over all gestational periods. In conclusion, embryo and fetal liver-bud-derived cells during the periods of 12.5 and 14.5 days, showed expression profile of progenitor cells, cell activity and hematopoietic function in culture.
Asunto(s)
Ciclo Celular , Feto , Hígado , Animales , Supervivencia Celular , Células Cultivadas , Femenino , Feto/citología , Feto/embriología , Hígado/citología , Hígado/embriología , Masculino , Ratas , Ratas Endogámicas F344RESUMEN
SUMMARY: Clinically, avian medicine still finds it very difficult to treat wild patients due to the lack of reliable information about their anatomy and physiological parameters that make comparative analyzes possible. Considering that computed tomography (CT) is one of the most viable alternatives in radiography of the head, we describe the comparative anatomy of the beak in the Ara ararauna and the Ramphastos toco through this imaging modality and macroscopy analysis, providing additional information about their conformation and topography for clinical exams. Adult specimens of each were dissected after the CT and beaks were removed for macroscopic analyses. The macroscopic and tomographic findings for both species confirm the similarities in beak morphology and the dietary peculiarities of each.The CT scan proved to be a useful tool to visualize internal structures of the skull through a detailed virtopsy of regions that are difficult to access by the usual necropsy with a scalpel.
RESUMEN: Debido a la escasa información de la anatomía y de los parámetros fisiológicos que hacen posible los análisis comparativos en la medicina aviar, clínicamente resulta muy difícil el tratamiento de estos animales. Considerando que la tomografía computarizada es una buena alternativa, estudiamos la anatomía comparativa del pico en Ara ararauna y Toco Toucan utilizando imágenes y un análisis de macroscopía. Estos métodos proporcionaron información adicional referente a su conformación y topografía para el trabajo clínico. Después de analizar la tomografía se disecaron las muestras de especímenes adultos y se retiraron los picos para un análisis macroscópico. Los hallazgos macroscópicos y tomográficos para ambas especies corroboraron la relación morfológica entre los picos y las peculiaridades dietéticas de cada uno. Se demostró además, que la tomografía computarizada es una herramienta útil para visualizar las estructuras internas del cráneo de las aves, a través de una virtopsia detallada en áreas de difícil acceso durante una necropsia habitual.
Asunto(s)
Animales , Pico/anatomía & histología , Pico/diagnóstico por imagen , Aves/anatomía & histología , Anatomía Comparada , Cráneo/anatomía & histología , Cráneo/diagnóstico por imagen , Tomografía Computarizada por Rayos XRESUMEN
The hoary fox (Lycalopex vetulus,Lund, 1842) is the smallest Brazilian canid, whose weight varies between 2 and 4 kg, has a slender body, a small head, and a short and blackened snout. Despite being considered an endemic species, little is known about the hoary fox as it is one of the seven less studied canids in the world. Thus, this study aimed to describe the anatomy of the abdominal aorta artery of the hoary fox and to compare it with the pre-established literature data in domestic canids. For this purpose, we used two adult hoary foxes without definite age. We collected the corpses of these animals along roadsides of Catalão-GO, being later fixed and conserved in a 10% formalin solution. The results showed that the abdominal aorta in hoary fox is at the ventral face of the lumbar region vertebral bodies, being slightly displaced to the left of the median plane. The first branch is visceral, named celiac artery, followed by a paired parietal branch: the phrenic abdominal arteries. The third and fourth branches are the cranial mesenteric arteries and the right and left are the renal arteries, respectively. The posterior branches of the renal arteries are equally visceral, paired, being called testicular arteries. Distal to the latter, both the caudal mesenteric artery and deep circumflex iliac arteries originate. Finally, two large external iliac arteries and its terminal branches composed of internal iliac arteries and the median sacral artery originate. In addition, five pairs of lumbar arteries originate alongside the abdominal segment of the aorta. Considering these findings, it is possible to conclude that despite the hoary fox being a wild animal, the anatomy of its abdominal aorta is very similar to that of domestic canids.(AU)
A raposa-do-campo (Lycalopex vetulus,Lund, 1842) é o menor canídeo brasileiro, cujo peso varia entre 2 e 4 quilos, possui corpo esguio, a cabeça é pequena, focinho curto e enegrecido. Considerada uma espécie endêmica, pouco se sabe a seu respeito, e é um dos sete canídeos menos estudados no mundo. Assim, o presente estudo teve o objetivo de descrever a anatomia da parte abdominal da artéria aorta em raposa-do-campo e comparar com dados literários pré-estabelecidos de canídeos domésticos. Para a realização deste estudo foram utilizados dois exemplares de raposa-do-campo, adultos, sem idade definida. Os cadáveres dos animais foram recolhidos às margens de rodovias no entorno da Catalão-Goiás, fixados em solução aquosa de formol a 10% e conservados na mesma solução. Os resultados mostraram que a aorta abdominal da raposa-do-campo está localizada sobre a face ventral dos corpos vertebrais da região lombar, levemente deslocada para a esquerda do plano mediano. O primeiro ramo é visceral, denominado artéria celíaca, seguido por um ramo parietal, pareado, as artérias frênico-abdominais. O terceiro e quarto ramos são a artéria mesentérica caudal e as artérias renais direita e esquerda, respectivamente. Os ramos posteriores das artérias renais são igualmente viscerais, pareados, denominados artérias testiculares. Distal à essas últimas, originam-se a artéria mesentérica caudal e as artérias circunflexas ilíacas profundas. Finalmente surgem duas grandes artérias ilíacas externas e os ramos terminais compostos pelas artérias ilíacas internas e artéria sacral mediana. Ao longo do trajeto da aorta abdominal, cinco pares de artérias lombares se originam da face dorsal. Considerando esses achados, pode ser concluído que a anatomia da aorta abdominal da raposa-do-campo é muito similar àquela de canídeos domésticos, embora ela seja um animal silvestre. (AU)