RESUMEN
Characterized as one of the most potent biosurfactants, surfactin is a cyclic lipopeptide synthesized by several strains of Bacillus genus. The aim of this review was to present the physicochemical and structural properties of surfactin and to demonstrate advances and applications of this biosurfactant for health and environmental biotechnology. Further, this review also focused on toxicological effects of surfactin on in vivo and in in vitro systems. The hydrophobic nature of surfactin enables interaction with membrane-bound phospholipids and indicates the ability of the molecule to act as a new weapon with respect to therapeutic and environmental properties. Seeking to avoid environmental contamination produced by widespread use of synthetic surfactants, surfactin emerges as a biological control agent against pathogen species owing to its antibacterial and antiviral properties. In addition, the mosquitocidal activity of surfactin was suggested as new strategy to control disease vectors. The current findings warrant future research to assess the toxicity of surfactin to enable an optimizing anticancer therapy and to seek refined methodologies, including nanotechnology techniques, to allow for an improved delivery of the biogenic molecule on target cells.
Asunto(s)
Bacillus/química , Proteínas Bacterianas/química , Lipopéptidos/química , Péptidos Cíclicos/química , Tensoactivos/química , Proteínas Bacterianas/farmacología , Proteínas Bacterianas/toxicidad , Biotecnología , Salud Ambiental , Lipopéptidos/farmacología , Lipopéptidos/toxicidad , Péptidos Cíclicos/farmacología , Péptidos Cíclicos/toxicidad , Tensoactivos/farmacología , Tensoactivos/toxicidadRESUMEN
An ideal wound dressing ensures a moist environment around the wound area and absorbs exudates from the wound surface. Topical application of bromelain to incised wounds has been shown to reprogram the wound microenvironment to promote effective tissue repair. Combining the characteristics of hydrogels and bromelain is therefore of great interest. Herein, we describe the development of a hydrogel, formulated using alginate and Arabic gum, for bromelain loading and release. The hydrogel formulation was evaluated using response surface methodology, considering the pH value and the concentration of alginate and Arabic gum. Bromelain loading and release were evaluated based on passive diffusion. Differential scanning calorimetry and Fourier transform infrared spectroscopy were performed to confirm bromelain immobilization in the hydrogel. The final hydrogel formulation had a swelling ratio of 227â% and incorporated 19â% of bromelain from a bromelain solution. Bromelain immobilization in the hydrogel was the result of hydrogen bond formation and was optimal at 4â°C after 4 h of contact. This evidence suggests that bromelain entrapment into a hydrogel is a promising strategy for the development of wound dressings that support the debridement of burns and wounds.
Asunto(s)
Alginatos , Bromelaínas/administración & dosificación , Liberación de Fármacos , Goma Arábiga , Bromelaínas/metabolismo , Composición de Medicamentos , Ácido Glucurónico , Ácidos Hexurónicos , Hidrogel de Polietilenoglicol-DimetacrilatoRESUMEN
This works reports the purification of bromelain extracted from Ananas comosus industrial residues by ethanol purification, its partial characterization from the crude extract as well as the ethanol purified enzyme, and its application onto poly(N-isopropylacrylamide)-co-acrylamide hydrogels. Bromelain was recovered within the 30-70 % ethanol fraction, which achieved a purification factor of 3.12-fold, and yielded more than 90 % of its initial activity. The resulting purified bromelain contained more than 360 U · mg(-1), with a maximum working temperature of 60â°C and pH of 8.0. Poly(N-isopropylacrylamide)-co-acrylamide hydrogels presented a swelling rate of 125 %, which was capable of loading 56 % of bromelain from the solution, and was able to release up to 91 % of the retained bromelain. Ethanol precipitation is suitable for bromelain recovery and application onto poly(N-isopropylacrylamide)-co-acrylamide hydrogels based on its processing time and the applied ethanol prices.
Asunto(s)
Acrilamida , Resinas Acrílicas , Ananas/química , Bromelaínas/administración & dosificación , Preparaciones de Acción Retardada , Hidrogeles , Bromelaínas/química , Bromelaínas/aislamiento & purificación , Hidrogeles/químicaRESUMEN
Landfill gas (LFG) is related to the biochemical processes generating heat and releasing CH4, CO2, and other gases in lower concentrations, which result in environmental impacts and risk of local explosion. Thermal infrared imagery (TIR) is employed to detect CH4 leakage as a risk control approach. However, the challenge for LFG leakage detection using TIR is establishing a relation between the gas flux and the ground temperature. This study evaluates the problem of a heated gas flowing through a porous medium column where the upward surface exchanges heat by radiation and convection to the environment. A heat transfer model that considers the upward LFG flow is proposed, and a sensibility analysis is developed to relate the flux to the ground temperature level in the condition of non-income solar radiation. An explicit equation to predict CH4 fugitive flow as a function of temperature anomalies of the ground was presented for the first time. The results show that the predicted ground surface temperatures are consistent with the literature's experimental observations. Moreover, the model was complementarily applied to a Brazilian landfill, with in situ TIR measurements in an area with a slightly fractured cover. In this field observation, the predicted CH4 flux was around 9025 g m-2 d-1. Model limitations concerning the soil homogeneity, the transient variation of atmospheric conditions or local pressure, and soil temperature difference in low-flux conditions (related to TIR-cameras accuracy) require further validation. Results could help landfill monitoring in conditions of a high-temperature ground anomaly in dry seasons.
Asunto(s)
Metano , Eliminación de Residuos , Metano/análisis , Instalaciones de Eliminación de Residuos , Gases/análisis , Temperatura , Suelo , Eliminación de Residuos/métodosRESUMEN
The waste-to-energy (WTE) valorization pathway of Caryocar brasiliense (pequi) seeds was investigated via pyrolysis, gasification, and transesterification to understand its potential as biochar, syngas, and biodiesel. First, the pyrolysis (300-700 °C) was conducted in N2 atmosphere for pequi seeds (PS) and pequi seeds without its extractives (PSWE), characterizing its biochar properties. The PSWE was then gasified at 1000 °C under O2/N2, O2/CO2/N2 and O2/H2O/N2 atmospheres to evaluate the characteristics of the producer gas. The PS extractives were then transesterified and characterized for biodiesel production. Finally, a multiple-criteria decision analysis assessed the PS products' potential within the thermochemical routes. The results evidenced better biochar (up to 22.29% HHV enhancement, higher mass and energy yield, up to 75.9 and 85.5% reduction of O/C and H/C, respectively, and enriched N content) via PSWE pyrolysis than PS considering biofuel application and optimistic perceptions for soil amendment. This indicates that the preceding extraction of vegetal fat from PS strengthens the WTE by including further processing of extracted oil. The produced syngas under O2/H2O/N2 gasification atmosphere showed better applicability as a biofuel (16.37 MJ·kg-1 lower heating value, 107.33% cold gas efficiency, and 113.55% carbon conversion efficiency) with up to 24% higher success rate. The transesterification of the extractives revealed its potential (98% conversion rate) for use as feedstock for in situ power generation, or blended for biodiesel production. The results provide insights into the circular economy in agro-extractivist communities that may support Brazil's small and medium agro-food industries with their energy demands.
Asunto(s)
Biocombustibles , Carbón Orgánico , Biomasa , Carbón Orgánico/química , PirólisisRESUMEN
Surfactants have been continuously detected within aquatic environments as a consequence of their use on a global scale. Lipopeptides are biosurfactants naturally produced by Bacillus subtilis that have been explored as green alternatives. The assessment of ecotoxicological parameters of synthetic and biogenic surfactants are required for evaluating toxicity values and to verify the eco-friendly behaviour of the biological compounds. This study aimed to conduct toxicity testing for different surfactants - sodium dodecyl sulphate and Triton X-100 - and biosurfactants - surfactin, iturin and fengycin - at different concentrations using Daphnia magna as model organism and Dendrocephalus brasiliensis as alternative test species for monitoring of pollutants in tropical freshwaters. According results, both species showed high sensitivity for the anionic compound SDS concerning the recommended dosage use, exhibiting EC50-48h values of 24.1 and 15.4â¯mg/L for D. magna and D. brasiliensis, respectively. Although the biological source, surfactin showed the lower safety behaviour among the biogenic surfactants, while iturin and fengycin revealed very low toxicity effects on both organisms. Besides, data exhibited a higher responsiveness of D. brasiliensis for all tested compounds in comparison to D. magna, highlighting the importance of this species for monitoring of pollutants in tropical and subtropical environments.
Asunto(s)
Daphnia/efectos de los fármacos , Ecotoxicología/métodos , Tensoactivos/toxicidad , Pruebas de Toxicidad , Contaminantes Químicos del Agua/toxicidad , Animales , Agua Dulce , Lipopéptidos/toxicidad , Octoxinol/toxicidad , Dodecil Sulfato de Sodio/toxicidadRESUMEN
Abstract L-Asparaginase (L-ASNase) is a biopharmaceutical used for acute lymphoblastic leukaemia (ALL) treatment, dramatically increasing the patients' chance of cure. However, its production and distribution in developing countries were disrupted because of its low profitability, which caused great concern among patients. This study evaluates the feasibility of combining fractional precipitation and aqueous two-phase systems (ATPS) to purify L-ASNase from a low-grade product, commercially known as Acrylaway® L. The ATPS purification results were not particularly expressive compared to the two-step purification process composed of ethanol precipitation and gel filtration, which was able to recover the target molecule with a purification factor over 5 fold. Thus, we studied a purification process capable of manufacturing pharmaceutical grade L-ASNase from a commercially available low-grade raw material; however, improvements regarding its throughput must be achieved, and high purity is the first step to apply it as a new biopharmaceutical product. The proposed process could pose as a short-time solution to mitigate its shortage while a cost-effective production plant is being developed.
Asunto(s)
Asparaginasa/aislamiento & purificación , Precipitación Fraccionada/métodos , Antineoplásicos/aislamiento & purificación , Estudios de Factibilidad , Cromatografía en Gel , Análisis Costo-BeneficioRESUMEN
Given the importance of protease's worldwide market, the determination of optimum conditions and the development of a standard protocol are critical during selection of a reliable method to determine its bioactivity. This paper uses quality control theory to validate a modified version of a method proposed by Charney and Tomarelli in 1947. The results obtained showed that using azocasein substrate bromelain had its optimum at 45°C and pH 9 (Glycine-NaOH 100 mM). We also quantified the limit of detection (LoD) and limit of quantification (LoQ) in the above-mentioned optimum (0.072 and 0.494 mg·mL(-1) of azocasein, resp.) and a calibration curve that correlates optical density with the amount of substrate digested. In all analysed samples, we observed a significant decrease in response after storage (around 17%), which suggests its use must be immediately after preparation. Thus, the protocol presented in this paper offers a significant improvement, given that subjective definitions are commonly used in the literature and this simple mathematical approach makes it clear and concise.
Asunto(s)
Bromelaínas/química , Caseínas/química , Proteolisis/efectos de los fármacos , Bromelaínas/farmacología , Caseínas/farmacología , Concentración de Iones de Hidrógeno , Límite de Detección , Especificidad por SustratoRESUMEN
Bromelain is a set of proteolytic enzymes found in pineapple (Ananas comosus) tissues such as stem, fruit and leaves. Because of its proteolytic activity, bromelain has potential applications in the cosmetic, pharmaceutical, and food industries. The present study focused on the recovery of bromelain from pineapple peel by liquid-liquid extraction in aqueous two-phase micellar systems (ATPMS), using Triton X-114 (TX-114) and McIlvaine buffer, in the absence and presence of electrolytes CaCl2 and KI; the cloud points of the generated extraction systems were studied by plotting binodal curves. Based on the cloud points, three temperatures were selected for extraction: 30, 33, and 36°C for systems in the absence of salts; 40, 43, and 46°C in the presence of KI; 24, 27, and 30°C in the presence of CaCl2 . Total protein and enzymatic activities were analyzed to monitor bromelain. Employing the ATPMS chosen for extraction (0.5 M KI with 3% TX-114, at pH 6.0, at 40°C), the bromelain extract stability was assessed after incorporation into three cosmetic bases: an anhydrous gel, a cream, and a cream-gel formulation. The cream-gel formulation presented as the most appropriate base to convey bromelain, and its optimal storage conditions were found to be 4.0 ± 0.5°C. The selected ATPMS enabled the extraction of a biomolecule with high added value from waste lined-up in a cosmetic formulation, allowing for exploration of further cosmetic potential.
Asunto(s)
Ananas/enzimología , Bromelaínas/química , Bromelaínas/aislamiento & purificación , Fraccionamiento Químico/métodos , Cosméticos/química , Micelas , BiotecnologíaRESUMEN
The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity.