RESUMEN
Hydraulic fracturing extracts oil and gas through the injection of water and proppants into subterranean formations. These injected fluids mix with the host rock formation and return to the surface as a complex wastewater containing salts, metals, and organic compounds, termed flowback and produced water (FPW). Previous research indicates that FPW is toxic to Daphnia magna (D. magna), impairing reproduction, molting, and maturation time; however, recovery from FPW has not been extensively studied. Species unable to recover have drastic impacts on populations on the ecological scale; thus, this study sought to understand if recovery from an acute 48 h FPW exposure was possible in the freshwater invertebrate, D. magna by using a combination of physiological and molecular analyses. FPW (0.75%) reduced reproduction by 30% and survivorship to 32% compared to controls. System-level quantitative proteomic analyses demonstrate extensive perturbation of metabolism and protein transport in both 0.25 and 0.75% FPW treatments after a 48 h FPW exposure. Collectively, our data indicate that D. magna are unable to recover from acute 48 h exposures to ≥0.25% FPW, as evidence of toxicity persists for at least 19 days post-exposure. This study highlights the importance of considering persisting effects following FPW remediation when modeling potential spill scenarios.
Asunto(s)
Fracking Hidráulico , Contaminantes Químicos del Agua , Animales , Daphnia/fisiología , Proteómica , Contaminantes Químicos del Agua/toxicidad , Contaminantes Químicos del Agua/análisis , AguaRESUMEN
Intertidal mussels are well adapted to withstand emersion from water during low tide, but they may be intermittently exposed to waterborne toxicants such as copper, which targets physiological processes including metabolism, ammonia excretion, and osmoregulation. To determine if copper exposure damages intertidal organisms' ability to tolerate tidal emersion, Mediterranean mussels (Mytilus galloprovincialis) were exposed to copper for 96 h followed by 6 h of emersion. Oxygen uptake increased after copper exposure which suggests that copper accumulation caused moderate stress in the mussels, but ammonia excretion and anaerobic metabolism were unaffected by mixed copper and emersion exposures. Shell composition analyses indicate that cycles of copper exposure and tidal emersion may affect bivalve shell growth, but copper deposition into shells may decrease the metal's overall toxicity. Results suggest that copper does not damage M. galloprovincialis's tolerance to tidal emersion, and insight is provided into the mussel's ability to overcome mixed stressor exposures.
Asunto(s)
Mytilus , Animales , Mytilus/metabolismo , Cobre/toxicidad , Cobre/metabolismo , Amoníaco/metabolismo , Agua , Adaptación FisiológicaRESUMEN
Hydraulic fracturing for oil and gas extraction produces large volumes of wastewater, termed flowback and produced water (FPW), that are highly saline and contain a variety of organic and inorganic contaminants. In the present study, FPW samples from ten hydraulically fractured wells, across two geologic formations were collected at various timepoints. Samples were analyzed to determine spatial and temporal variation in their inorganic composition. Results indicate that FPW composition varied both between formations and within a single formation, with large compositional changes occurring over short distances. Temporally, all wells showed a time-dependent increase in inorganic elements, with total dissolved solids increasing by up to 200,000 mg/L over time, primarily due to elements associated with salinity (Cl, Na, Ca, Mg, K). Toxicological analysis of a subset of the FPW samples showed median lethal concentrations (LC50) of FPW to the aquatic invertebrate Daphnia magna were highly variable, with the LC50 values ranging from 1.16% to 13.7% FPW. Acute toxicity of FPW significantly correlated with salinity, indicating salinity is a primary driver of FPW toxicity, however organic components also contributed to toxicity. This study provides insight into spatiotemporal variability of FPW composition and illustrates the difficulty in predicting aquatic risk associated with FPW.
Asunto(s)
Fracking Hidráulico , Animales , Daphnia , Epiclorhidrina , Dosificación Letal Mediana , AguaRESUMEN
During hydraulic fracturing, wastewaters - termed flowback and produced water (FPW) - are created as a by-product during hydrocarbon extraction. Given the large volumes of FPW that a single well can produce, and the history of FPW release to surface water bodies, it is imperative to understand the hazards that hydraulic fracturing and FPW pose to aquatic biota. Using rainbow trout embryos as model organisms, we investigated impacts to cardio-respiratory system development and function following acute (48 h) and sub-chronic (28-day) FPW exposure by examining occurrences of developmental deformities, rates of embryonic respiration (MO2), and changes in expression of critical cardiac-specific genes. FPW-exposed embryos had significantly increased rates of pericardial edema, yolk-sac edema, and tail/trunk curvatures at hatch. Furthermore, when exposed at three days post-fertilization (dpf), acute 5% FPW exposures significantly increased embryonic MO2 through development until 15 dpf, where a switch to significantly reduced MO2 rates was subsequently recorded. A similar trend was observed during sub-chronic 1% FPW exposures. Interestingly, at certain specific developmental timepoints, previous salinity exposure seemed to affect embryonic MO2; a result not previously observed. Following acute FPW exposures, embryonic genes for cardiac development and function were significantly altered, although at termination of sub-chronic exposures, significant changes to these same genes were not found. Together, our evidence of induced developmental deformities, modified embryonic MO2, and altered cardiac transcript expression suggest that cardio-respiratory tissues are toxicologically targeted following FPW exposure in developing rainbow trout. These results may be helpful to regulatory bodies when developing hazard identification and risk management protocols concerning hydraulic fracturing activities.
Asunto(s)
Fracking Hidráulico , Oncorhynchus mykiss , Contaminantes Químicos del Agua , Animales , Corazón , Aguas Residuales , AguaRESUMEN
Large stores of previously inaccessible hydrocarbons have become available due to the development of hydraulic fracturing technologies. During the hydraulic fracturing process, a mixture of water and proprietary additives is injected into geologic formations to release trapped hydrocarbons. After fracturing, injected water and fluid from the target formation return to the surface as flowback and produced water (FPW), a potentially toxic byproduct of hydraulic fracturing activities. FPW is a complex mixture that contains chemical additives present in the initial injection fluid as well as salts, metals, and a variety of organic compounds. As a result, FPW composition can be highly variable across wells from different geological formations, methods of fracturing and well development, and well age. The present study sought to determine if FPW sourced from four wells (O, P, U, V) located on the same well pad within the Montney Formation have similar levels of acute and chronic toxicity to the freshwater invertebrate, Daphnia magna. Minimal differences in the estimated 48 h LC50 concentrations were observed among the studied wells. Long-term, 21 d exposures to ≤2% FPW revealed differences in the level of lethality between wells, including complete mortality in daphnids exposed to 2% well O by day 9. No sublethal effects were observed as a result of exposure to FPW from wells P, U or V; however, a large impairment of reproductive traits and molting behaviour were detected after exposure to 0.75% well O FPW. These results indicate that FPW sourced from wells on the same well pad cannot be considered the same in terms of chemical composition or toxicity, an important distinction to make for risk assessment practices.
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Daphnia , Geología , Animales , Agua DulceRESUMEN
In aquatic toxicology, methods that are chosen for exposures have profound consequences on experimental outcomes and thus can skew policy initiatives. For example, as compared to single-organism exposures, toxicity test results of group exposures may be impacted by confounding factors such as social interactions between animals or individual variation in accumulation rates. To test for differences in organismal response between group and individual toxicological exposures, we exposed Daphnia magna to copper and subsequently compared the toxicity (median lethal concentration or LC50) between groups and individuals. Results suggested that water chemistry had a larger effect on experimental outcomes than the number of animals exposed in the same tank. Methodological decisions with respect to replication type can affect toxicity tests, and LC50s calculated using different exposure types (such as group and individual exposures) may not be comparable.
Asunto(s)
Contaminantes Químicos del Agua , Animales , Cobre , Daphnia , Humanos , Dosificación Letal Mediana , Pruebas de Toxicidad , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidadRESUMEN
Hydraulic fracturing has become widely used in recent years to access vast global unconventional sources of oil and gas. This process involves the injection of proprietary mixtures of water and chemicals to fracture shale formations and extract the hydrocarbons trapped within. These injection fluids, along with minerals, hydrocarbons, and saline waters present within the formations being drilled into, return to the surface as flowback and produced water (FPW). FPW is a highly complex mixture, containing metals, salts and clay, as well as many organic chemicals, including polycyclic aromatic hydrocarbons such as phenanthrene. The present study sought to determine the effects of temperature on the accumulation of phenanthrene in rainbow trout (Oncorhynchus mykiss). This model organism resides in rivers overlapping the Montney and Duvernay formations, both highly developed formations for hydraulic fracturing. Rainbow trout acclimated to temperatures of 4, 13 and 17 °C were exposed to either 5% or 20% FPW, as well as saline mixtures representing the exact ionic content of FPW to determine the accumulation of radiolabelled 14C phenanthrene within the gill, gut, liver and gallbladder. FPW exposure reduced the overall accumulation of phenanthrene in a manner most often similar to high salinity exposure, indicating that the high ionic strength of FPW is the primary factor affecting accumulation. Accumulation was different at the temperature extremes (4 and 17 °C), although no consistent relationship was observed between temperature and accumulation across the observed tissues. These results indicate that several physiological responses occur as a result of FPW exposure and water temperature change which dictate phenanthrene uptake, particularly in the gills. Temperature (and seasonality) alone cannot be used to model the potential accumulation of polycyclic aromatic hydrocarbons after FPW spills.
Asunto(s)
Fracking Hidráulico , Oncorhynchus mykiss , Fenantrenos , Contaminantes Químicos del Agua , Animales , Temperatura , Agua , Contaminantes Químicos del Agua/análisisRESUMEN
Hydraulic fracturing creates large volumes of flowback and produced water (FPW). The waste is a complex mixture of organic and inorganic constituents. Although the acute toxicity of FPW to freshwater organisms has been studied, few have attempted to discern the interaction between organic and inorganic constituents within this matrix and its role in toxicity. In the present study, bioaccumulation assays (7-d uptake and 7-d elimination period) with FPW (1% dilution) were conducted with the freshwater oligochaete, Lumbriculus variegatus, to evaluate the toxicokinetics of inorganic elements. To evaluate the interacting role of organics, bioaccumulation of elements in unmodified FPW was compared to activated carbon treated FPW (AC-modified). Differences in uptake and elimination rates as well as elimination steady state concentrations between unmodified and AC-modified treatments indicated that the organics play an important role in the uptake and depuration of inorganic elements in FPW. These differences in toxicokinetics between treatments aligned with observed growth rates in the worms which were higher in the AC-modified treatment. Whether growth differences resulted from increased accumulation and changes in toxicokinetic rates of inorganics or caused by direct toxicity from the organic fraction of FPW itself is still unknown and requires further research.
Asunto(s)
Fracking Hidráulico , Oligoquetos , Contaminantes Químicos del Agua , Animales , Bioacumulación , Sedimentos Geológicos , Aguas Residuales , Agua , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidadRESUMEN
Public concern is heightened around flowback and produced water (FPW) generated by the hydraulic fracturing process. FPW is a complex mix of organic and inorganic solutes derived from both the injected hydraulic fracturing fluid and interactions with the subsurface lithology. Few studies to date have systematically investigated the composition of FPW or its individual components. Here, we provide the first systematic characterization of the composition of the solids associated with FPW by analyzing samples from three wells drilled into the Duvernay Formation in Alberta, Canada. The FPW initially returned to the surface with high total dissolved solids (greater than 170 000 mg L-1) and enriched with Fe(ii), silica, sulfate, barium, and strontium. The solids form two distinct phases once the FPW reached the surface: (1) silica-enriched Fe(iii) oxyhydroxides, and (2) a barite-celestine solid solution. We hypothesize that the precipitation of the amorphous silica-enriched Fe(iii) oxyhydroxide is a two-step process, where first the silica precipitates as a function of the cooling of the FPW from elevated subsurface temperatures to ambient surface temperatures. Next, the silica acts as a template for the precipitation of Fe(iii) oxyhydroxide as the diffusion of oxygen into the subsurface causes oxidation of aqueous Fe(ii). The barite-celestine solid solution precipitates solely as a function of cooling. Elevated dissolved Fe concentrations in FPW and modeled saturation indices from five North American shale plays (Marcellus, Fayetteville, Barnett, Bakken, and Denver-Julesburg) indicate that solids similar to those found in Duvernay FPW, specifically Fe(iii) oxyhydroxides, barite and quartz, are likely to occur. With the solids known to carry a significant portion of FPW's toxicity and organic contaminant load, the development of new treatment technologies, such as the oxidation of the Fe(ii) in FPW, may increase FPW reuse and reduce the environmental risk posed by FPW.