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1.
Int J Sports Med ; 44(14): 1043-1048, 2023 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-36452984

RESUMEN

Empirical observations support that the addition of a plastic strip - also known as Randall foils - on the top edge of a rowing blade improves rowing efficiency during the cycle propulsive phase. The aim of the current study was to analyze the effect of using big blades with and without Randall foils on rowing performance. Twenty experienced rowers performed two 90 s tethered rowing bouts (with and without Randall foils) to assess their impact on force production and physiologic variables. All tests were randomized and a repeated measure design was used to compare experimental conditions. Higher values of peak and mean peak forces (479.4±134.7 vs. 423.2±153.0, d=0.83 and 376.5±101.4 vs. 337.1±113.3 N, d=0.68), peak oxygen uptake (47.9±7.5 vs. 45.3±7.3 mL∙kg-1∙min-1, d=0.19), peak blood lactate concentration (7.9±1.6 vs. 6.9±1.7 mmol∙L-1, d=0.16), blood lactate increasing speed (0.08±0.01 vs. 0.07±0.06 [(mmol·L-1)·s-1], d=0.27) and lactic anaerobic energy (27.4±7.9 vs. 23.4±8.1 kJ, d=0.23) were found for big blades with vs. without Randall foils, p<0.05. The current data suggest that the Randall foils can positively affect rowing performance.


Asunto(s)
Deportes Acuáticos , Humanos , Lactatos , Consumo de Oxígeno
2.
Molecules ; 28(6)2023 Mar 09.
Artículo en Inglés | MEDLINE | ID: mdl-36985492

RESUMEN

Beer is one of the most consumed beverages worldwide with unique organoleptic properties. Bitterness and astringency are well-known key features and, when perceived with high intensity, could lead to beer rejection. Most studies on beer astringency and bitterness use sensory assays and fail to study the molecular events that occur inside the oral cavity responsible for those perceptions. This work focused on deepening this knowledge based on the interaction of salivary proteins (SP) and beer phenolic compounds (PCs) and their effect toward these two sensory attributes. The astringency and bitterness of four different beers were assessed by a sensory panel and were coupled to the study of the SP changes and PC profile characterization of beers. The human SP content was measured before (basal) and after each beer intake using HPLC analysis. The beers' PC content and profile were determined using Folin-Ciocalteu and LC-MS spectrometry, respectively. The results revealed a positive correlation between PCs and astringency and bitterness and a negative correlation between SP changes and these taste modalities. Overall, the results revealed that beers with higher PC content (AAL and IPA) are more astringent and bitter than beers with a lower PC content (HL and SBO). The correlation results suggested that an increase in whole SP content, under stimulation, should decrease astringency and bitterness perception. No correlation was found between the changes in specific families of SP and astringency and bitterness perception.


Asunto(s)
Astringentes , Gusto , Humanos , Astringentes/análisis , Cerveza/análisis , Percepción del Gusto , Fenoles/análisis , Proteínas y Péptidos Salivales/análisis
3.
Eur J Appl Physiol ; 120(9): 2005-2018, 2020 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-32591994

RESUMEN

PURPOSE: Breath-by-breath energy expenditure during open water swimming has not yet been explored in an ecological environment. This study aimed to investigate and compare energetics and kinematics of 5 km swimming, in both swimming pool and open water conditions. METHODS: Through four independent studies, oxygen uptake ([Formula: see text]2) kinetics, heart rate (HR), blood lactate concentration ([La-]) and glucose level (BGL), metabolic power ([Formula: see text]), energy cost (C) and kinematics were assessed during 5 km front crawl trials in a swimming pool and open water conditions. A total of 38 competitive open water swimmers aged 16-27 years volunteered for this four part investigation: Study A (pool, ten females, 11 males), Study B (pool, four females, six males), Study C (pool case study, one female) and Study D (open water, three females, four males). RESULTS: In the swimming pool, swimmers started with an above average swimming speed (v), losing efficiency along the 5 km, despite apparent homeostasis for [La-], BGL, [Formula: see text]2, [Formula: see text] and C. In open water, swimmers started the 5 km with a below average v, increasing the stroke rate (SR) in the last 1000 m. In open water, [Formula: see text]2 kinetics parameters, HR, [La-], BGL, respiratory exchange ratio and C were affected by the v and SR fluctuations along the 5 km. CONCLUSIONS: Small fluctuations were observed for energetic variables in both conditions, but changes in C were lower in swimming pool than in open water. Coaches should adjust the training plan accordingly to the specificity of open water swimming.


Asunto(s)
Metabolismo Energético/fisiología , Natación/fisiología , Adolescente , Adulto , Rendimiento Atlético/fisiología , Femenino , Glucosa/metabolismo , Frecuencia Cardíaca/fisiología , Humanos , Cinética , Ácido Láctico/sangre , Masculino , Consumo de Oxígeno/fisiología , Respiración , Agua , Adulto Joven
4.
Int J Sports Med ; 41(5): 318-327, 2020 May.
Artículo en Inglés | MEDLINE | ID: mdl-31975360

RESUMEN

The anaerobic threshold (AnT) seems to be not only a physiologic boundary but also a transition after which swimmers technique changes, modifying their biomechanical behaviour. We expanded the AnT concept to a biophysical construct in the four conventional swimming techniques. Seventy-two elite swimmers performed a 5×200 m incremental protocol in their preferred swimming technique (with a 0.05 m·s-1 increase and a 30 s interval between steps). A capillary blood samples were collected from the fingertip and stroke rate (SR) and length (SL) determined for the assessment of [La], SR and SL vs. velocity inflexion points (using the interception of a pair of linear and exponential regression curves). The [La] values at the AnT were 3.3±1.0, 3.9±1.1, 2.9±1 .34 and 4.5±1.4 mmol·l-1 (mean±SD) for front crawl, backstroke, breaststroke and butterfly, and its corresponding velocity correlated highly with those at SR and SL inflection points (r=0.91-0.99, p<0.001). The agreement analyses confirmed that AnT represents a biophysical boundary in the four competitive swimming techniques and can be determined individually using [La] and/or SR/SL. Blood lactate increase speed can help characterise swimmers' anaerobic behaviour after AnT and between competitive swimming techniques.


Asunto(s)
Umbral Anaerobio/fisiología , Natación/fisiología , Adolescente , Fenómenos Biomecánicos , Conducta Competitiva/fisiología , Femenino , Humanos , Ácido Láctico/sangre , Extremidad Inferior/fisiología , Masculino , Acondicionamiento Físico Humano , Análisis y Desempeño de Tareas , Extremidad Superior/fisiología , Adulto Joven
5.
Molecules ; 25(15)2020 Jul 29.
Artículo en Inglés | MEDLINE | ID: mdl-32751126

RESUMEN

The call for health-promoting nutraceuticals and functional foods containing bioactive compounds is growing. Among the great diversity of functional phytochemicals, polyphenols and, more recently, bioactive peptides have stood out as functional compounds. The amount of an ingested nutrient able to reach the bloodstream and exert the biological activity is a critical factor, and is affected by several factors, such as food components and food processing. This can lead to unclaimed interactions and/or reactions between bioactive compounds, which is particularly important for these bioactive compounds, since some polyphenols are widely known for their ability to interact and/or precipitate proteins/peptides. This review focuses on this important topic, addressing how these interactions could affect molecules digestion, absorption, metabolism and (biological)function. At the end, it is evidenced that further research is needed to understand the true effect of polyphenol-bioactive peptide interactions on overall health outcomes.


Asunto(s)
Suplementos Dietéticos , Péptidos/farmacología , Polifenoles/farmacología , Humanos , Péptidos/química , Proteínas de Plantas/química , Plantas/química , Polifenoles/química , Proteínas/química , Investigación
6.
Molecules ; 25(11)2020 Jun 02.
Artículo en Inglés | MEDLINE | ID: mdl-32498458

RESUMEN

Astringency and bitterness are organoleptic properties widely linked to tannin compounds. Due to their significance to food chemistry, the food industry, and to human nutrition and health, these tannins' taste properties have been a line of worldwide research. In recent years, significant advances have been made in understanding the molecular perception of astringency pointing to the contribution of different oral key players. Regarding bitterness, several polyphenols have been identified has new agonists of these receptors. This review summarizes the last data about the knowledge of these taste properties perceived by tannins. Ultimately, tannins' astringency and bitterness are hand-in-hand taste properties, and future studies should be adapted to understand how the proper perception of one taste could affect the perception of the other one.


Asunto(s)
Astringentes/análisis , Taninos/análisis , Gusto , Análisis de los Alimentos , Industria de Alimentos , Humanos , Polifenoles/química
7.
Anal Biochem ; 537: 60-62, 2017 11 15.
Artículo en Inglés | MEDLINE | ID: mdl-28867502

RESUMEN

Fly fruit Drosophila melanogaster (DM) has been extensively employed as an in vivo model system to study pesticides toxicity. Pesticide administration to the fly traditionally involves feeding in an agar-gelled feed fly's medium (AM). However, AM method has several limitations such as uncertainty regarding the bioavailability and amount of pesticides ingested. And also high manipulation of the treated flies. We developed a new method of exposure the flies to pesticides, called Continuous Liquid Feeding (CLF). This method successfully delivers food to the flies at much higher concentrations than the AM method, and requires little manipulation of flies under treatment.


Asunto(s)
Drosophila melanogaster/efectos de los fármacos , Métodos de Alimentación , Plaguicidas/toxicidad , Agar/química , Animales , Drosophila melanogaster/fisiología , Ingestión de Alimentos , Femenino , Glicina/análogos & derivados , Glicina/toxicidad , Locomoción/efectos de los fármacos , Masculino , Glifosato
8.
Crit Rev Food Sci Nutr ; 57(5): 937-948, 2017 Mar 24.
Artículo en Inglés | MEDLINE | ID: mdl-25897713

RESUMEN

Polyphenols have been the subject of numerous research over the past years, being referred as the nutraceuticals of modern life. The healthy properties of these compounds have been associated to a natural chemoprevention of 21st century major diseases such as cancer and neurodegenerative diseases (e.g. Parkinson's and Alzheimer's). This association led to an increased consumption of foodstuffs rich in these compounds such as red wine. Related to the ingestion of polyphenols are the herein revised sensorial properties (astringency and bitterness) which are not still pleasant. This review intends to be an outline both at a sensory as a molecular level of the mechanisms underlying astringency and bitterness of polyphenols. Up-to-date knowledge of this matter is discussed in detail.


Asunto(s)
Polifenoles/química , Vino/análisis , Carbohidratos de la Dieta/análisis , Proteínas en la Dieta/química , Flavonoides/química , Humanos , Saliva/metabolismo , Proteínas y Péptidos Salivales/metabolismo , Taninos/química , Gusto
9.
Molecules ; 22(2)2017 Feb 14.
Artículo en Inglés | MEDLINE | ID: mdl-28216567

RESUMEN

Wine, and particularly red wine, is a beverage with a great chemical complexity that is in continuous evolution. Chemically, wine is a hydroalcoholic solution (~78% water) that comprises a wide variety of chemical components, including aldehydes, esters, ketones, lipids, minerals, organic acids, phenolics, soluble proteins, sugars and vitamins. Flavonoids constitute a major group of polyphenolic compounds which are directly associated with the organoleptic and health-promoting properties of red wine. However, due to the insufficient epidemiological and in vivo evidences on this subject, the presence of a high number of variables such as human age, metabolism, the presence of alcohol, the complex wine chemistry, and the wide array of in vivo biological effects of these compounds suggest that only cautious conclusions may be drawn from studies focusing on the direct effect of wine and any specific health issue. Nevertheless, there are several reports on the health protective properties of wine phenolics for several diseases such as cardiovascular diseases, some cancers, obesity, neurodegenerative diseases, diabetes, allergies and osteoporosis. The different interactions that wine flavonoids may have with key biological targets are crucial for some of these health-promoting effects. The interaction between some wine flavonoids and some specific enzymes are one example. The way wine flavonoids may be absorbed and metabolized could interfere with their bioavailability and therefore in their health-promoting effect. Hence, some reports have focused on flavonoids absorption, metabolism, microbiota effect and overall on flavonoids bioavailability. This review summarizes some of these major issues which are directly related to the potential health-promoting effects of wine flavonoids. Reports related to flavonoids and health highlight some relevant scientific information. However, there is still a gap between the knowledge of wine flavonoids bioavailability and their health-promoting effects. More in vivo results as well as studies focused on flavonoid metabolites are still required. Moreover, it is also necessary to better understand how biological interactions (with microbiota and cells, enzymes or general biological systems) could interfere with flavonoid bioavailability.


Asunto(s)
Flavonoides/química , Vino , Antocianinas/química , Antioxidantes/química , Disponibilidad Biológica , Enfermedades Cardiovasculares/prevención & control , Diabetes Mellitus/prevención & control , Etanol/química , Flavonoides/farmacocinética , Microbioma Gastrointestinal , Humanos , Neoplasias/prevención & control , Enfermedades del Sistema Nervioso/prevención & control , Obesidad/prevención & control , Polifenoles/química
10.
Langmuir ; 31(30): 8392-401, 2015 Aug 04.
Artículo en Inglés | MEDLINE | ID: mdl-26162056

RESUMEN

The interaction between phenolic compounds and salivary proteins is considered the basis of the poorly understood phenomenon of astringency. Furthermore, this interaction is an important factor in relation to their bioavailability. In this work, interactions between anthocyanin and human salivary protein fraction were studied by mass spectrometry (MALDI-TOF-MS and FIA-ESI-MS) and saturation-transfer difference (STD) NMR spectroscopy. Anthocyanins were able to interact with saliva proteins. The dissociation constant (KD) between malvidin 3-glucoside and salivary proline-rich proteins was 1.92 mM for the hemiketal form (pH 3.4) and 1.83 mM for the flavylium cation (pH 1.0). New soluble complexes between these salivary proteins and malvidin 3-glucoside were identified for the first time.


Asunto(s)
Antocianinas/química , Proteínas y Péptidos Salivales/química , Adulto , Femenino , Voluntarios Sanos , Humanos , Espectroscopía de Resonancia Magnética , Masculino , Espectrometría de Masas , Adulto Joven
11.
Nutrients ; 16(4)2024 Feb 16.
Artículo en Inglés | MEDLINE | ID: mdl-38398875

RESUMEN

Food allergies are becoming ever more prevalent around the world. This pathology is characterized by the breakdown of oral tolerance to ingested food allergens, resulting in allergic reactions in subsequent exposures. Due to the possible severity of the symptoms associated with this pathology, new approaches to prevent it and reduce associated symptoms are of utmost importance. In this framework, dietary phenolic compounds appear as a tool with a not fully explored potential. Some phenolic compounds have been pointed to with the ability to modulate food allergies and possibly reduce their symptoms. These compounds can modulate food allergies through many different mechanisms, such as altering the bioaccessibility and bioavailability of potentially immunogenic peptides, by modulating the human immune system and by modulating the composition of the human microbiome that resides in the oral cavity and the gastrointestinal tract. This review deepens the state-of-the-art of the modulation of these mechanisms by phenolic compounds. While this review shows clear evidence that dietary supplementation with foods rich in phenolic compounds might constitute a new approach to the management of food allergies, it also highlights the need for further research to delve into the mechanisms of action of these compounds and decipher systematic structure/activity relationships.


Asunto(s)
Hipersensibilidad a los Alimentos , Humanos , Alérgenos , Alimentos , Dieta , Fenoles/farmacología , Boca/patología
12.
Int J Sports Physiol Perform ; : 1-7, 2024 Jun 19.
Artículo en Inglés | MEDLINE | ID: mdl-38897581

RESUMEN

PURPOSE: To compare the kinematic profile of 2 underwater pullout breaststroke techniques. METHODS: Sixteen swimmers (9 men, 20.67 [2.71] y old; 7 women, 18.86 [0.83] y old) performed 3 × 25-m breaststroke using 2 pullout breaststroke techniques: Fly-Kick first and Combined. A speedometer was used to assess the peak and the mean velocity during the glide, propulsion, and recovery phases of both techniques, as well as for the total underwater sequence. The underwater distance was retrieved from video footage and was considered for each pullout technique. The range of motion of the knee during the fly-kick was also retrieved, and the time to complete the 25 m was considered the performance outcome, accompanied by the mean velocity, stroke rate, stroke length, and stroke index. RESULTS: Velocity-time series showed different profiles between pullout techniques (P ≤ .05) mostly in the glide and propulsion phases for males and females, respectively. The mean velocity of 25 m was shown to be greater in females when using the Fly-Kick first technique (P = .05, d = 0.36). Greater values in total underwater distance and knee range of motion were also observed for this technique in both cohorts. Conclusions: Female swimmers presented a higher performance when using the Fly-Kick first technique. Different kinematic profiles arise when swimmers use different underwater pullout techniques where the Fly-Kick first may allow them to reach higher kinematical standard.

13.
Food Chem ; 448: 139153, 2024 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-38569410

RESUMEN

Salivary proteins precipitation by interaction with polyphenols is the major mechanism for astringency. However, alternative mechanisms seem involved in the perception of different subqualities of astringency. In this study, adsorption of four astringent agents to in vitro oral models and their sensory properties were assessed. Overall, green tea infusion and tannic acid have shown a higher adsorption potential for models with oral cells and absence of saliva. Alum and grape seed extract presented higher adsorption in models with presence of oral cells and saliva. Multiple factor analysis suggested that adsorption may represent important mechanisms to elicit the astringency of alum. Models including saliva, were closely associated with overall astringency and aggressive subquality. Models with cells and absent saliva were closely associated with greenness, suggesting a taste receptor mechanism involvement in the perception. For the first time a correlation between an oral-cell based assay and astringency sensory perception was shown.

14.
J Clin Periodontol ; 40(11): 1001-6, 2013 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-24164568

RESUMEN

AIM: The aim of this cross-sectional study was to evaluate the prevalence of destructive periodontal disease and its risk indicators in adult Kiriri Indians from Northeast Brazil. MATERIALS AND METHODS: Full-mouth periodontal examinations were performed on a sample of 215 Indians (≥19 years). Bivariate analyses and logistic models were applied to assess associations between periodontitis and its putative risk factors. RESULTS: Prevalence of clinical attachment loss of ≥3, ≥5 and ≥7 mm was 97.8%, 63.8% and 30.8% respectively. Percentage of teeth per individual showing clinical attachment loss of ≥3, ≥5 and ≥7 mm was 49.8%, 18.4% and 8.0% respectively. After adjustment for covariates, individuals aged ≥35 years (OR = 5.83, 95% CI: 3.09-11.00; p < 0.001), men (OR = 2.18, 95% CI: 1.15-4.11; p = 0.02) and diabetics (OR = 3.92, 95% CI 1.03-14.99; p = 0.05) had a higher risk for destructive periodontitis, classified according to the CDC/AAP case definition. CONCLUSION: Though periodontitis was highly prevalent in Kiriri Indians, only few teeth showed advanced disease, and periodontitis was associated with higher age, male sex and diabetes. A public health action that includes programs of prevention and treatment targeting high-risk groups is vital to improve the periodontal status of this population.


Asunto(s)
Indígenas Sudamericanos/estadística & datos numéricos , Periodontitis/epidemiología , Adulto , Factores de Edad , Anciano , Brasil/epidemiología , Brasil/etnología , Estudios Transversales , Índice CPO , Caries Dental/epidemiología , Restauración Dental Permanente/estadística & datos numéricos , Diabetes Mellitus/epidemiología , Escolaridad , Femenino , Humanos , Renta/estadística & datos numéricos , Masculino , Persona de Mediana Edad , Pérdida de la Inserción Periodontal/epidemiología , Bolsa Periodontal/epidemiología , Prevalencia , Factores de Riesgo , Factores Sexuales , Fumar/epidemiología , Pérdida de Diente/epidemiología , Adulto Joven
15.
J Sports Sci Med ; 12(4): 623-9, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-24421720

RESUMEN

The aim of this study was to characterize the backstroke swimming technique of 11-13 year-old swimmers when performing at very high intensity. A sample of 114 swimmers was divided into four groups regarding maturational and gender effect, who performed 25- m backstroke swimming at 50-m pace. Using two underwater cameras the general biomechanical parameters (speed, stroke rate, stroke length and stroke index), the arm stroke phases and two indexes of arm coordination (Index of Coordination 1, which characterizes the continuity between propulsive phases of each arm and Index of Coordination 2 that evaluates the simultaneity between the beginning of the pull of one arm and of the recovery of the other arm) were measured. Post- pubertal swimmers achieved higher values of speed (1.06 ± 0.14 and 1.18 ± 0.14 m·s(-1) for pubertal and 1.13 ± 0.14 and 1.24 ± 0.12 m·s(-1) for post-pubertal girl and boy swimmers, respectively), stroke length (1.64 ± 0.26 and 1.68 ± 0.25 m·cycle(-1) for pubertal and 1.79 ± 0.22 and 1.75 ± 0.27 m·cycle(-1) for post-pubertal girls and boys, respectively) and stroke index. Regar-ding genders, male were faster than female swimmers. Boys also showed a higher stroke rate and stroke index than girls, who achieved higher results in the ratio between stroke length and arm span. As it was expected, no hand lag time was noticed in young swimmers. Although no differences were noticed between genders, the Index of Coordination 1 was in catch-up mode (-9.89 ± 3.16 and -10.16 ± 3.60 % for girls and -9.77 ± 2.93 and -10.39 ± 2.44 % for boys pubertal and post-pubertal, respectively) and the Index of Coordination 2 was in superposition mode (1.86 ± 4.39 and 2.25 ± 2.25 % from girls and 1.72 ± 2.62 and 1.95 ± 2.95 % for boys, pubertal and post-pubertal, respectively). Key PointsYoung swimmers adopt the catch-up arm coordina-tion when swimming backstroke.These swimmers present lower stroking parameters then those published for older and higher level swimmers.No hand lag time at the thigh was noticed, meaning that young swimmers perform the final phase of their arm cycle without inducing discontinuity be-tween the propulsive actions of the two arms.

16.
J Agric Food Chem ; 71(4): 2070-2081, 2023 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-36652684

RESUMEN

It is well known that repeated exposure to phenolic compounds (PCs) raises astringency perception. However, the link between this increase and the oral cavity's interactions with salivary proteins (SPs) and other oral constituents is unknown. To delve deeper into this connection, a flavonoid-rich green tea extract was tested in a series of exposures to two oral cell-based models using a tongue cell line (HSC3) and a buccal mucosa cell line (TR146). Serial exposures show cumulative PC binding to all oral models at all concentrations of the green tea extract; however, the contribution for the first and second exposures varies. The tongue mucosal pellicle (HSC3-Mu-SP) may contribute more to first-stage astringency (retaining 0.15 ± 0.01 mg mL-1 PCs at the first exposure), whereas the buccal mucosal pellicle (TR146-Mu-SP) retained significantly less (0.08 ± 0.02 mg mL-1). Additionally, increased salivary volume (SV+), which simulates the stimulation of salivary flow brought by a food stimulus, significantly enhances PC binding, particularly for TR146 cells: TR46-Mu-SP_SV+ bound significantly higher total PC concentration (0.17 ± 0.02 mg mL-1) than the model without increased salivary volume TR146-Mu-SP_SV- (0.09 ± 0.03 mg mL-1). This could be associated with a higher contribution of these oral cells for astringency perception during repeated exposures. Furthermore, PCs adsorbed in the first exposure to cell monolayer models (+TR146 and +HSC3) change the profile of PCs bound to these models in the second exposure. Regarding the structure binding activity, PCs with a total higher number of hydroxyl groups were more bound by the models containing SP. Regarding the SP, basic proline-rich proteins (bPRPs) may be involved in the increased perception of astringency upon repeated exposures. The extent of bPRP precipitation by PCs in mucosal pellicle models for both cell lines (HSC3 and TR146) in the second exposure (76 ± 13 and 83 ± 6%, respectively) was significantly higher than in the first one (25 ± 14 and 5 ± 6%, respectively).


Asunto(s)
Astringentes , Flavonoides , Aspergillus fumigatus/metabolismo , Astringentes/química , Azoles , Farmacorresistencia Fúngica , Flavonoides/metabolismo , Proteínas Fúngicas/metabolismo , Fenoles/metabolismo , Saliva/química , Proteínas y Péptidos Salivales/metabolismo , Té/metabolismo , Boca
17.
Plants (Basel) ; 12(19)2023 Sep 26.
Artículo en Inglés | MEDLINE | ID: mdl-37836137

RESUMEN

The consumption of 'not-from-concentrate' (NFC) fruit juices can be a convenient and enjoyable way to incorporate the nutritional benefits and flavors of fruits into one's diet. This study will focus on the effect of production of juices from apple and pear fruits, by using centrifugal decanter and tangential filtration, on the profile of polyphenols as a valuable source of bioactive compounds. Likewise, by-products from the juice industry were characterized in order to understand the high-value-added potential based on their composition of polyphenols. Briefly, apple and apple juice showed great contents of chlorogenic acid (0.990 ± 0.021 mg/g of DW), the dihydrochalcone phloridzin (1.041 ± 0.062 mg/g of DW), procyanidins (0.733 ± 0.121 mg/g of DW) and quercetin derivatives (1.501 ± 0.192 mg/g of DW). Likewise, the most abundant compounds in pear and pear juices were chlorogenic acid (0.917 ± 0.021 mg/g of DW), caffeoylquinic acid (0.180 ± 0.029 mg/g of DW), procyanidins (0.255 ± 0.016 mg/g of DW) and quercetin derivatives (0.181 ± 0.004 mg/g of DW). Both temperature and tangential speed affect the amount of phenolic compounds in fruit juices, highlighting the need to control the technological process to obtain a more nutritious/healthier beverage. Overall, NFC juices arise as a better option when compared with concentrated juices. Furthermore, the higher yield of phenolic compounds found in fruit pomace clearly open new ways for upcycling this fruit by-product as a high-value-added ingredient.

18.
Pharmaceuticals (Basel) ; 16(6)2023 Jun 20.
Artículo en Inglés | MEDLINE | ID: mdl-37375852

RESUMEN

Lycopene is a carotenoid with potential use in the treatment of chronic illnesses. Here, different formulations of lycopene were studied: lycopene-rich extract from red guava (LEG), purified lycopene from red guava (LPG) and a self-emulsifying drug delivery system loaded with LPG (nanoLPG). The effects of administering orally various doses of LEG to hypercholesterolemic hamsters were evaluated regarding the liver function of the animals. The cytotoxicity of LPG in Vero cells was analyzed by a crystal violet assay and by fluorescence microscopy. In addition, nanoLPG was employed in stability tests. LPG and nanoLPG were tested for their cytotoxic effect on human keratinocytes and antioxidant capacity on cells in an endothelial dysfunction model in an isolated rat aorta. Finally, the effect of different nanoLPG concentrations on the expression of immune-related genes (IL-10, TNF-α, COX-2 and IFN-γ) from peripheral blood mononuclear cells (PBMC) using real-time PCR was also analyzed. Results suggest that LEG, despite not being able to improve blood markers indicative of liver function in hypercholesterolemic hamsters, reduced hepatic degenerative changes. Additionally, LPG did not show cytotoxicity in Vero cells. In relation to nanoLPG, the effects produced by heat stress evaluated by Dynamics Light Scattering (DLS) and visually were loss of color, texture change and phase separation after 15 days without interfering with the droplet size, so the formulation proved to be efficient in stabilizing the encapsulated lycopene. Although LPG and nanoLPG showed moderate toxicity to keratinocytes, which may be related to cell lineage characteristics, both revealed potent antioxidant activity. LPG and nanoLPG showed vasoprotective effects in aortic preparations. The gene expression assay indicates that, although no significant differences were observed in the expression of IL-10 and TNF-α, the PBMCs treated with nanoLPG showed a reduction in transcriptional levels of IFN-γ and an increased expression of COX-2. Thus, the work adds evidence to the safety of the use of lycopene by humans and shows that tested formulations, mainly nanoLPG due to its stability, stand out as promising and biosafe products for the treatment of diseases that have oxidative stress and inflammation in their etiopathology.

19.
Chem Senses ; 37(2): 191-8, 2012 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-22086902

RESUMEN

Astringency has been defined as a group of sensations involving dryness, tightening, and shrinking of the oral surface. It has been accepted that astringency is due to the tannin-induced interaction and/or precipitation of the salivary proline-rich proteins (PRPs) in the oral cavity, as a result of the ingestion of food products rich in tannins, for example, red wine. The sensory evaluation of astringency is difficult, and the existence of fast and reliable methods to its study in vitro is scarce. So, in this work, the astringency of red wine supplemented with oligomeric procyanidins (condensed tannins), and the salivary proteins (SP) involved in its development were evaluated by high-performance liquid chromatography analysis of human saliva after its interaction with red wine and by sensorial evaluation. The results show that for low concentration of tannins, the decrease of acidic PRPs and statherin is correlated with astringency intensity, with these families having a high relative complexation and precipitation toward condensed tannins comparatively to the other SP. However, for higher concentrations of tannins, the relative astringency between wines seems to correlate's to the glycosylated PRPs changes. This work shows for the first time that the several families of SP could be involved in different stages of the astringency development.


Asunto(s)
Boca/fisiología , Proantocianidinas/farmacología , Saliva/química , Proteínas Salivales Ricas en Prolina/metabolismo , Percepción del Gusto , Vino/análisis , Astringentes/farmacología , Cromatografía Líquida de Alta Presión , Glicosilación , Humanos , Boca/efectos de los fármacos , Saliva/efectos de los fármacos , Saliva/metabolismo
20.
Pediatr Exerc Sci ; 24(3): 409-19, 2012 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-22971557

RESUMEN

Our aim was to characterize front crawl swimming performed at very high intensity by young practitioners. 114 swimmers 11-13 years old performed 25 m front crawl swimming at 50 m pace. Two underwater cameras was used to assess general biomechanical parameters (velocity, stroke rate, stroke length and stroke index) and interarm coordination (Index of Coordination), being also identified each front crawl stroke phase. Swimmers presented lower values in all biomechanical parameters than data presented in studies conducted with older swimmers, having the postpubertal group closest values to adult literature due to their superior anthropometric and maturational characteristics. Boys showed higher velocity and stroke index than girls (as reported for elite swimmers), but higher stroke rate than girls (in opposition to what is described for adults). In addition, when considering the total sample, a higher relationship was observed between velocity and stroke length (than with stroke rate), indicating that improving stroke length is a fundamental skill to develop in these ages. Furthermore, only catch-up coordination mode was adopted (being evident a lag time between propulsion of the arms), and the catch and the pull phases presented the highest and smallest durations, respectively.


Asunto(s)
Rendimiento Atlético/fisiología , Destreza Motora/fisiología , Natación/fisiología , Adolescente , Factores de Edad , Fenómenos Biomecánicos , Niño , Femenino , Humanos , Masculino , Pubertad , Factores Sexuales , Maduración Sexual , Extremidad Superior/fisiología , Grabación en Video
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