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1.
Appl Environ Microbiol ; 53(8): 1880-4, 1987 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-3116935

RESUMEN

After Clostridium botulinum type G organisms and toxin were identified in necropsy specimens in cases of unexplained death in adults and infants (O. Sonnabend, W. Sonnabend, R. Heinzle, T. Sigrist, R Dirnhofer, and U. Krech, J. Infect. Dis. 143:22-27, 1981), extensive research to detect C. botulinum type G in soil samples from Switzerland was done. A total of 41 specimens from virgin soil and from cultivated land were examined for the presence of C. botulinum type G and other toxin types. Because of the lack of the lipase marker in type G, the detection of C. botulinum type G was based on the demonstration of type G organisms in enrichment cultures by a type G-specific enzyme-linked immunosorbent assay to detect both the type G toxin and antigen; enrichment cultures in which type G toxin or antigen was identified by enzyme-linked immunosorbent assay were then tested by a type G-specific gel immunodiffusion agar procedure. This method not only isolated strains of type G but also strains of Clostridium subterminale, a nontoxigenic variant of C. botulinum type G. As a consequence of the observed cross-reactions caused by strains of C. subterminale within this test system, all isolates of type G had to be definitively confirmed by mouse bioassay. The sequential steps of these methods seem to be very useful for detecting C. botulinum type G organisms. C. botulinum type G strains were isolated in five soil samples from different locations in close association with cultivated land.(ABSTRACT TRUNCATED AT 250 WORDS)


Asunto(s)
Clostridium botulinum/aislamiento & purificación , Microbiología del Suelo , Antígenos Bacterianos/análisis , Toxinas Botulínicas/análisis , Clostridium botulinum/inmunología , Reacciones Cruzadas , Ensayo de Inmunoadsorción Enzimática , Inmunodifusión , Suiza
2.
Lancet ; 1(8423): 237-41, 1985 Feb 02.
Artículo en Inglés | MEDLINE | ID: mdl-2857317

RESUMEN

As part of a programme to exclude infection as the cause of death in infants who died suddenly and unexpectedly necropsies were carried out on 70 such infants. In 11 cases (15%), a pathological diagnosis could be made at necropsy; in 9 of these, causative bacteria or viruses were found. The 59 cases in which the cause of death could not be found had histological features characteristic of sudden infant death syndrome (SIDS). Botulinum toxin was found in 9 SIDS cases (15%). 8 of these infants had botulinum toxin and organisms of different types (A, B, C, F, G) in the contents of the ileojejunum or colon. 4 of them also had toxin in the serum. No botulinum toxin or organisms were found in the 11 infants who died of identified causes or 18 other infants who died in hospital of known causes.


Asunto(s)
Botulismo/microbiología , Enfermedades Intestinales/microbiología , Muerte Súbita del Lactante/microbiología , Toxinas Botulínicas/análisis , Clostridium botulinum/aislamiento & purificación , Femenino , Humanos , Lactante , Recién Nacido , Masculino , Muerte Súbita del Lactante/etiología , Muerte Súbita del Lactante/patología , Virosis/microbiología
3.
Lancet ; 1(8529): 357-61, 1987 Feb 14.
Artículo en Inglés | MEDLINE | ID: mdl-2880164

RESUMEN

A 27-year-old man with type F botulism (classification undetermined) had two episodes of botulinum toxaemia with identification of botulinum toxin and Clostridium botulinum organisms in faecal specimens during a three-month stay in hospital. Between these clinical episodes neither toxin nor Cl botulinum could be demonstrated. The illness was severe with quadriplegia, respiratory insufficiency, and bowel paralysis. In addition the patient had sensory abnormalities and a raised protein in the cerebrospinal fluid. The results demonstrate for the first time in detail an intestinal colonisation with and multiplication of C botulinum organisms and in-vivo production of toxin in an adult. The clinical findings at first pointed to Guillain-Barré syndrome, and it is suggested that patients with this syndrome should be examined for botulinum toxin in serum and for toxin and organisms in stool.


Asunto(s)
Toxinas Botulínicas/sangre , Botulismo/diagnóstico , Clostridium botulinum/aislamiento & purificación , Enfermedades Intestinales/diagnóstico , Adulto , Clostridium botulinum/clasificación , Diagnóstico Diferencial , Humanos , Masculino , Polirradiculoneuropatía/diagnóstico
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