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INTRODUCTION: Because paradoxical sleep deprivation (PSD) induces a catabolic hormone profile, we sought to evaluate the morphology of the tibialis anterior (TA) muscle and testosterone and corticosterone levels of paradoxical sleep-deprived rats. METHODS: Three study groups of rats were established: the first group was sleep deprived for 96 h; the second group was also sleep deprived for 96 h, but then returned to their home-cage and allowed to sleep for the next 96 h; and the third group was the control group, with a normal sleep cycle. RESULTS: PSD reduced the weight and fiber cross-sectional area of the TA muscle. Moreover, PSD enhanced plasma corticosterone and reduced serum testosterone levels. The 96 h of sleep after PSD was sufficient to restore partially the morphology of TA, while hormones returned to basal levels. CONCLUSION: PSD induces hormonal alterations that may mediate muscle atrophy.
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Atrofia Muscular/etiología , Privación de Sueño/complicaciones , Análisis de Varianza , Animales , Modelos Animales de Enfermedad , Masculino , Ratas , Ratas WistarRESUMEN
In the present review, we identify which instruments and parameters are used for sleep quality monitoring in individual sport athletes and which deï¬nitions were used for sleep quality parameters in this literature ï¬eld. Systematic searches for articles reporting the qualitative markers related to sleep in team sport athletes were conducted in PubMed, Scopus and Web of Science online databases. The systematic review followed the Preferred Reporting Items for Systematic Reviews. The initial search returned 3316 articles. After the removal of duplicate articles, eligibility assessment, 75 studies were included in this systematic review. Our main ï¬ndings were that the most widely used measurement instruments were Actigraphy (25%), Rating Likert Scales (16%) and Sleep Diary (13%). On sleep quality parameters (Sleep duration = 14%; Wake after sleep onset = 14%; Sleep Quality = 12%; Sleep Effciency = 11% and Sleep Latency = 9%), the main point is that there are different deï¬nitions for the same parameters in many cases reported in the literature. We conclude that the most widely used instruments for monitoring sleep quality were Actigraphy, Likert scales and Sleep diary. Moreover, the deï¬nitions of sleep parameters are inconsistent in the literature, hindering the understanding of the sleep-sport performance relationship.
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BACKGROUND: CrossFit is recognized as one of the fastest growing high-intensity functional training modes in the world. However, scientific data regarding the practice of CrossFit is sparse. Therefore, the objective of this study is to analyze the findings of scientific literature related to CrossFit via systematic review and meta-analysis. METHODS: Systematic searches of the PubMed, Web of Science, Scopus, Bireme/MedLine, and SciELO online databases were conducted for articles reporting the effects of CrossFit training. The systematic review followed the PRISMA guidelines. The Oxford Levels of Evidence was used for all included articles, and only studies that investigated the effects of CrossFit as a training program were included in the meta-analysis. For the meta-analysis, effect sizes (ESs) with 95% confidence interval (CI) were calculated and heterogeneity was assessed using a random-effects model. RESULTS: Thirty-one articles were included in the systematic review and four were included in the meta-analysis. However, only two studies had a high level of evidence at low risk of bias. Scientific literature related to CrossFit has reported on body composition, psycho-physiological parameters, musculoskeletal injury risk, life and health aspects, and psycho-social behavior. In the meta-analysis, significant results were not found for any variables. CONCLUSIONS: The current scientific literature related to CrossFit has few studies with high level of evidence at low risk of bias. However, preliminary data has suggested that CrossFit practice is associated with higher levels of sense of community, satisfaction, and motivation.
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The aim of this study was to identify the possible association between biochemical markers of exercise addiction and affective parameters in a sample of athletes during 2weeks of withdrawal exercise. Eighteen male runners were distributed into a control group (n=10) composed of runners without exercise addiction symptoms and an exercise addiction group (n=8) composed of runners with exercise addiction symptoms. The volunteers performed a baseline evaluation that included affective questionnaires, blood samples, body composition and an aerobic test performed at ventilatory threshold I. After the baseline evaluation, the groups started an exercise withdrawal period that was sustained for 2weeks. During exercise withdrawal, an actigraph accelerometer was used to monitor the movement index, and CK and LDH were measured in blood samples to validate the non-exercise practice. At the end of the exercise withdrawal period, a blood collection, aerobic test and mood scale was performed in the re-test. The results showed that at the end of the experimental protocol, when compared with the control group, the exercise addiction group showed an increase in depression, confusion, anger, fatigue and decreased vigor mood that improved post-exercise, along with low levels of anandamide at all time-points evaluated and a modest increase in ß-endorphin post-exercise. Moreover, the exercise addiction group showed a decrease in oxygen consumption and respiratory exchange ratio after the exercise withdrawal period, which characterized a detraining phenomenon. Our data suggest that a 2-week withdrawal exercise period resulted in an increase of negative mood in exercise addiction; additionally, exercise addiction showed low levels of anandamide.
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Afecto , Ácidos Araquidónicos/sangre , Atletas/psicología , Endocannabinoides/sangre , Ejercicio Físico/psicología , Alcamidas Poliinsaturadas/sangre , Adulto , Biomarcadores/sangre , Depresión/fisiopatología , Ejercicio Físico/fisiología , Humanos , Masculino , Consumo de Oxígeno , betaendorfina/sangreRESUMEN
BACKGROUND: Paradoxical sleep deprivation activates the sympathetic nervous system and the hypothalamus-pituitary-adrenal axis, subsequently interfering with the cardiovascular system. The beneficial effects of resistance training are related to hemodynamic, metabolic and hormonal homeostasis. We hypothesized that resistance training can prevent the cardiac remodeling and dysfunction caused by paradoxical sleep deprivation. METHODS: Male Wistar rats were distributed into four groups: control (C), resistance training (RT), paradoxical sleep deprivation for 96 hours (PSD96) and both resistance training and sleep deprivation (RT/PSD96). Doppler echocardiograms, hemodynamics measurements, cardiac histomorphometry, hormonal profile and molecular analysis were evaluated. RESULTS: Compared to the C group, PSD96 group had a higher left ventricular systolic pressure, heart rate and left atrium index. In contrast, the left ventricle systolic area and the left ventricle cavity diameter were reduced in the PSD96 group. Hypertrophy and fibrosis were also observed. Along with these alterations, reduced levels of serum testosterone and insulin-like growth factor-1 (IGF-1), as well as increased corticosterone and angiotensin II, were observed in the PSD96 group. Prophylactic resistance training attenuated most of these changes, except angiotensin II, fibrosis, heart rate and concentric remodeling of left ventricle, confirmed by the increased of NFATc3 and GATA-4, proteins involved in the pathologic cardiac hypertrophy pathway. CONCLUSIONS: Resistance training effectively attenuates cardiac dysfunction and hormonal imbalance induced by paradoxical sleep deprivation.
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Ecocardiografía Doppler , Hemodinámica , Hipertrofia Ventricular Izquierda , Condicionamiento Físico Animal , Privación de Sueño , Remodelación Ventricular , Angiotensina II/sangre , Animales , Corticosterona/sangre , Frecuencia Cardíaca , Ventrículos Cardíacos/diagnóstico por imagen , Ventrículos Cardíacos/metabolismo , Ventrículos Cardíacos/fisiopatología , Hipertrofia Ventricular Izquierda/sangre , Hipertrofia Ventricular Izquierda/diagnóstico por imagen , Hipertrofia Ventricular Izquierda/etiología , Factor I del Crecimiento Similar a la Insulina/metabolismo , Masculino , Ratas , Ratas Wistar , Privación de Sueño/sangre , Privación de Sueño/diagnóstico por imagen , Privación de Sueño/fisiopatología , Testosterona/sangreRESUMEN
Objective. Describe multicompartmental changes in the fat and various muscle fiber types, as well as the hormonal profile and metabolic rate induced by SD in rats. Methods. Twenty adult male Wistar rats were equally distributed into two groups: experimental group (EG) and control group (CG). The EG was submitted to SD for 96 h. Blood levels of corticosterone (CORT), total testosterone (TESTO), insulin like growth factor-1 (IGF-1), and thyroid hormones (T3 and T4) were used to assess the catabolic environment. Muscle trophism was measured using a cross-sectional area of various muscles (glycolytic, mixed, and oxidative), and lipolysis was inferred by the weight of fat depots from various locations, such as subcutaneous, retroperitoneal, and epididymal. The metabolic rate was measured using oxygen consumption ([Formula: see text]O2) measurement. Results. SD increased CORT levels and decreased TESTO, IGF-1, and T4. All fat depots were reduced in weight after SD. Glycolytic and mixed muscles showed atrophy, whereas atrophy was not observed in oxidative muscle. Conclusion. Our data suggest that glycolytic muscle fibers are more sensitive to atrophy than oxidative fibers during SD and that fat depots are reduced regardless of their location.
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Sleep deprivation (SD) can induce muscle atrophy. We aimed to investigate the changes underpinning SD-induced muscle atrophy and the impact of this condition on rats that were previously submitted to resistance training (RT). Adult male Wistar EPM-1 rats were randomly allocated into 1 of 5 groups: control, sham, SD (for 96 h), RT, and RT+SD. The major outcomes of this study were muscle fiber cross-sectional area (CSA), anabolic and catabolic hormone profiles, and the abundance of select proteins involved in muscle protein synthesis and degradation pathways. SD resulted in muscle atrophy; however, when SD was combined with RT, the reduction in muscle fiber CSA was attenuated. The levels of IGF-1 and testosterone were reduced in SD animals, and the RT+SD group had higher levels of these hormones than the SD group. Corticosterone was increased in the SD group compared with the control group, and this increase was minimized in the RT+SD group. The increases in corticosterone concentrations paralleled changes in the abundance of ubiquitinated proteins and the autophagic proteins LC3 and p62/SQSTM1, suggesting that corticosterone may trigger these changes. SD induced weight loss, but this loss was minimized in the RT+SD group. We conclude that SD induced muscle atrophy, probably because of the increased corticosterone and catabolic signal. High-intensity RT performed before SD was beneficial in containing muscle loss induced by SD. It also minimized the catabolic signal and increased synthetic activity, thereby minimizing the body's weight loss.
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Contracción Muscular , Músculo Esquelético/metabolismo , Atrofia Muscular/prevención & control , Entrenamiento de Fuerza , Privación de Sueño/terapia , Animales , Autofagia , Corticosterona/metabolismo , Modelos Animales de Enfermedad , Factor I del Crecimiento Similar a la Insulina/metabolismo , Masculino , Proteínas Musculares/metabolismo , Músculo Esquelético/patología , Músculo Esquelético/fisiopatología , Atrofia Muscular/metabolismo , Atrofia Muscular/patología , Atrofia Muscular/fisiopatología , Complejo de la Endopetidasa Proteasomal/metabolismo , Proteolisis , Ratas Wistar , Transducción de Señal , Privación de Sueño/metabolismo , Privación de Sueño/patología , Privación de Sueño/fisiopatología , Testosterona/metabolismo , UbiquitinaciónRESUMEN
Fundamento: O treinamento resistido promove benefícios à saúde cardiovascular, a qual é influenciada pela privação de sono. Objetivo: Investigar o efeito prévio do treinamento resistido de alta intensidade sobre a contratilidade miocárdica de ratos privados de sono paradoxal. Métodos: Quarenta ratos machos Wistar foram distribuídos nos grupos controle (CTRL), treinamento resistido (TRES), privação de sono paradoxal por 96 horas (PSP96) e treinamento resistido seguido de privação de sono paradoxal por 96 horas (TRES/PSP96). O treinamento resistido foi de alta intensidade, por 8 semanas, 5x/semana. Vinte e quatro horas após a última sessão de treinamento, os grupos PSP96 e TRES/PSP96 foram submetidos ao protocolo de privação de sono paradoxal e em seguida foi realizado o estudo in vitro da mecânica contrátil do músculo papilar isolado. Resultados: Em comparação ao CTRL, os grupos PSP96 e TRES/PSP96 apresentaram menor comprimento do músculo papilar e aumento da área de secção transversa. Associado a essas alterações, verificou-se a diminuição das derivadas temporais da força na contração e relaxamento em todas as condições avaliadas. Somente o grupo PSP96 apresentou redução da tensão de repouso e lentidão no tempo de relaxamento, sendo este último atenuado pelo treinamento resistido prévio. Conclusão: O treinamento resistido prévio à PSP foi parcialmente protetor contra as alterações contráteis do músculo papilar, minimizando a lentidão no tempo de relaxamento. Assim, o caráter de alta intensidade do protocolo adotado parece não proteger plenamente o tecido cardíaco frente a PSP
Background: Resistance training promotes cardiovascular health benefits that may affected by sleep deprivation. Objective: To evaluate the effect of high-intensity resistance training on myocardial contractility in rats subsequently subjected to paradoxical sleep deprivation. Methods: Forty male Wistar rats were distributed into control group (CTRL), resistance training (REST), 96-hour paradoxical sleep deprivation (PSD96) and resistance training followed by 96-hour paradoxical sleep deprivation (REST/PSD96). The animals underwent highintensity resistance training for 8 weeks, 5x/week. Twenty-four hours after the last training session, the PSD96 and REST/PSD96 groups were submitted to paradoxical sleep deprivation, which was followed by the in vitro study of isolated papillary muscle contractile mechanics. Results: In comparison with the CTRL group, a lower papillary muscle length and increased cross sectional area were found in PSD96 and RETS/PSD96, which were associated with decreased temporal parameters of contraction force and relaxation. Decreased resting tension and slowing of relaxation time were found in the PSD96 group only. This effect was attenuated by previous resistance training. Conclusion: Resistance training partially prevented contractile changes induced by PSD, minimizing the slowing in relaxation time. Thus, high-intensity exercise seems to not fully protect the cardiac tissue from PSD-induced effects