Acute exercise activates the AHR in peripheral blood mononuclear cells in an intensity-dependent manner.

The kynurenine pathway of tryptophan degradation generates several metabolites such as kynurenine or kynurenic acid that serve as endogenous ligands of the aryl hydrocarbon receptor (AHR). Due to its distinct biological roles particularly modulating the immune system, the AHR is a current therapeutic target across different inflammation-related diseases. Here, we show an acute exercise-induced increase in AHR ligand availability on a systemic level and a kynurenine pathway activation in peripheral blood mononuclear cells (PBMCs). Concurrently, the AHR is activated in PBMCs following acute exercise. Exercise effects on both, kynurenic acid and AHR activation in PBMCs were greater in response to high-intensity interval exercise (50 min., six three-minute intervals á 90% V̇O2peak, and three-minute intervals at 50% V̇O2peak in between) compared to workload-matched moderate intensity continuous exercise (50 min.). In conclusion, these data indicate a novel mechanistic link how exercise modulates the immune system through the kynurenine pathway-AHR axis, potentially underlying exercise-induced benefits in various chronic diseases.

Preconditioning Strategies Before Maximum Clean Performance in Female Weightlifters.

ABSTRACT: Kelekian, GK, Zaras, N, Stasinaki, AN, Spiliopoulou, P, Karampatsos, G, Bogdanis, G, and Terzis, G. Preconditioning strategies before maximum clean performance in female weightlifters. J Strength Cond Res 36(8): 2318-2321, 2022-The aim of the study was to examine the acute effect of performing clean pulls with either 85% or 120% one repetition maximum (1RM), on subsequent clean performance, in female Olympic weightlifters. Eight competitive female Olympic weightlifters (age, 22.9 ± 5.8 years; height, 1.67 ± 0.06 m; training experience, 6.1 ± 3.9 years; clean performance, 66.3 ± 3.9 kg), performed clean pulls either with 85% or with 120% 1RM, 3 minutes before their maximum effort in clean, in 2 different days, in a counterbalanced order. The rate of perceived exertion (RPE) was provided after each intervention. Body composition (dual x-ray absorptiometry), countermovement jumping (CMJ) performance, leg press isometric rate of force development (RFD), and peak force (PF) were also evaluated on different days. Clean performance was increased by 6.1 ± 3.6% ( p = 0.002) after preconditioning with 85% 1RM and 4.7 ± 3.1% ( p = 0.004) after 120% 1RM. Rate of perceived exertion was lower after preconditioning with 85% 1RM, compared with 120% 1RM ( p = 0.015). No significant correlation was found between the changes in clean performance after 85% or 120% 1RM, with lean body mass, CMJ performance, and isometric leg press RFD, and leg press PF. These data suggest that lifting either 85% or 120% 1RM in clean pulls is an effective strategy for acute increase of clean performance in female Olympic weightlifters, whereas the use of 85% 1RM load may be performed with less exertion.

Effect of Concurrent Power Training and High-Intensity Interval Cycling on Muscle Morphology and Performance.

ABSTRACT: Spiliopoulou, P, Zaras, N, Methenitis, S, Papadimas, G, Papadopoulos, C, Bogdanis, GC, and Terzis, G. Effect of concurrent power training and high-intensity interval cycling on muscle morphology and performance. J Strength Cond Res 35(9): 2464-2471, 2021-The aim of the study was to examine the effect of performing high-intensity interval cycling on muscle morphology and performance immediately after power training (PT). Twenty healthy female physical education students were assigned into 2 training groups. One group performed PT, and the other group performed the same PT followed by high-intensity interval aerobic training on a cycle ergometer (PTC). Training was performed 3 days per week for 6 weeks. Countermovement jump (CMJ) height and CMJ power, half-squat maximal strength (1 repetition maximum), maximum aerobic power, vastus lateralis muscle fiber composition, and cross-sectional area (CSA) were evaluated before and after the intervention. Countermovement jump height increased after PT (10.1 ± 6.6%, p = 0.002) but not after PTC (-5.1 ± 10.5%, p = 0.099), with significant difference between groups (p = 0.001). Countermovement jump power increased after PT (4.5 ± 4.9%, p = 0.021) but not after PTC (-2.4 ± 6.4, p = 0.278), with significant difference between groups (p = 0.017). One repetition maximum increased similarly in both groups. Muscle fiber composition was not altered after either PT or PTC. Vastus lateralis muscle fiber CSA increased significantly and similarly after both PT (I: 16.9 ± 16.2%, p = 0.035, ΙΙΑ: 12.7 ± 10.9%, p = 0.008,ΙΙΧ: 15.5 ± 17.1%, p = 0.021) and PTC (Ι: 18.0 ± 23.7%, p = 0.033,ΙΙΑ: 18.2 ± 11.4%, p = 0.001,ΙΙΧ: 25.5 ± 19.6%, p = 0.003). These results suggest that the addition of high-intensity interval cycling to PT inhibits the anticipated increase in jumping performance induced by PT per se. This inhibition is not explained by changes in muscle fiber type composition or vastus lateralis muscle fiber CSA adaptations.

Effect of Inter-Repetition Rest Vs. Traditional Resistance Training on The Upper Body Strength Rate of Force Development and Triceps Brachii Muscle Architecture.

The purpose of the study was to examine the effect of seven-week inter-repetition rest vs. traditional resistance training on upper body maximum strength, the rate of force development and triceps brachii muscle architecture. Sixteen male participants were equally assigned into the inter-repetition rest and the traditional group. In both groups, training included the bench press exercise performed with 4 sets of 6 maximum repetitions, two training sessions per week. Twenty-second inter-repetition rest was employed for the inter-repetition rest group only. Measurements before and after the training period included maximum strength in the bench press, the isometric upper body rate of force development and peak force and triceps brachii muscle architecture. Maximum strength increased significantly in both groups (inter-repetition rest group: 21.5 ± 5.7% vs. traditional group: 13.5 ± 7.2%, p < 0.05), however, the maximum strength percentage increase was greater in the inter-repetition rest group compared to the traditional group (p = 0.027). Upper body isometric peak force increased only after inter-repetition rest training (10.7 ± 10.3%, p = 0.009). The rate of force development remained unchanged for both groups (p > 0.05), although percentage changes in time frames of 0-80 and 0-100 milliseconds were greater for the inter-repetition rest group compared to the traditional training group (p = 0.024 and p = 0.044, respectively). Triceps brachii thickness increased similarly for both groups (p < 0.05). These results suggest that inter-repetition rest may induce greater increases in maximum strength and the rate of force development compared to traditional training during the initial weeks of resistance training.

Exercise-Induced Changes in Tumor Growth via Tumor Immunity.

Immunity in the tumor microenvironment plays a central role in tumor development. Cytotoxic immune cells act against tumors, while tumors manage to trigger immunosuppressive mechanisms for defense. One bout of physical exercise acutely regulates the immune system inducing short-term redistribution of immune cells among body organs. Repeated acute immune cell mobilization with continuing exercise training results in long-term adaptations. These long-term exercise-induced changes in the immune system arise both in healthy and in diseased populations, including cancer patients. Recent preclinical studies indicate that physical exercise may have a positive impact on intra-tumoral immune cell processes, resulting in tumor suppression. This short narrative review describes the effect of physical exercise on tumor growth as detected via changes in tumor immunity. Research evidence shows that exercise may improve tumor-suppressive functions and may reduce tumor-progressive responses and mechanisms of immune cells, controlling tumor development. Specifically, it seems that exercise in rodents triggers shifts in tumor infiltration of macrophages, neutrophils, natural killer cells, cytotoxic and regulatory T lymphocytes, resulting in tumor suppression. These recent promising data suggest that physical exercise could be combined with anticancer immunotherapies, although exercise parameters like intensity, duration, and frequency need to be evaluated in more detail. More research is needed to investigate the effect of exercise in other immune cell subtypes and their possible connection with tumor growth, whilst information from human tumors is also required.

Rate of Force Development, Muscle Architecture, and Performance in Elite Weightlifters.

PURPOSE: The purpose of the present study was to investigate the relationship between weightlifting performance and the rate of force development (RFD), muscle architecture, and body composition in elite Olympic weightlifters. METHODS: Six male Olympic weightlifters (age 23.3 [3.4] y, body mass 88.7 [10.2] kg, body height 1.76 [0.07] m, snatch 146.7 [15.4] kg, clean and jerk 179.4 [22.1] kg), all members of the national team, participated in the study. Athletes completed a 16-week periodized training program aiming to maximize their performance at the national competition event. Measurements, including maximal strength (1-repetition maximum) in snatch, clean and jerk, back and front squat, isometric leg press RFD and peak force, countermovement jump, vastus lateralis muscle architecture, and body composition, were performed before and after the training period. RESULTS: Weightlifting performance increased significantly after training (P < .05). Leg press RFD increased only in time windows of 0 to 200 and 0 to 250 milliseconds after training (8.9% [8.5%] and 9.4% [7.7%], respectively, P < .05) while peak force remained unaltered (P < .05). Front squat strength increased significantly (P < .05), while countermovement jump power increased 2.3% (2.1%) (P < .05). No changes were observed for muscle architecture and lean body mass (P > .05). Significant correlations were observed between performance in snatch and clean and jerk with isometric leg press RFD, at all time windows, as well as with lean body mass and squat 1-repetition maximum. CONCLUSIONS: These results suggest that regular examination of RFD, lean body mass, and lower extremities' 1-repetition maximum may be useful performance predictors in elite Olympic weightlifters.

Weak Association Between Vastus Lateralis Muscle Fiber Composition and Fascicle Length in Young Untrained Females.

The aim of the study was to investigate the relationships between vastus lateralis muscle fiber length and fiber type composition in individuals with minimal exposure to systematic resistance/power training. In sixty female physical education students (age: 21.03 ± 2.1 years, body weight: 59.8 ± 9.7 kg, body height: 166.2 ± 6.5 cm), with no experience in systematic training, lean body mass, VL muscle architecture and fiber composition type, countermovement jumping (CMJ) performance, and isometric leg press rate of force development were evaluated. Data were analyzed for all participants, as well as two equally numbered groups assigned according to their maximum countermovement jumping power (High-Power or Low-Power group). Significant but low correlations were found between type II muscle fiber percentage and fascicle length (N = 60, p < 0.05). Significant correlations were found between type IIa and IIx muscle fiber percentage cross-sectional area (%CSA) and fascicle length (N = 60; r = 0.321, and r = 0.378; respectively, p < 0.05). These correlations were higher for the High-Power group (r = 0.499, and r = 0.522; respectively, p < 0.05), and lower, and nonsignificant, for the Low-Power group. The best predictor of strength/power performance was the lean body mass of the lower extremities (r = 0.389-0.645, p < 0.05). These results suggest that in females with minimal exposure to systematic training, fascicle length may be weakly linked with type II fiber areas, only in females with high-power profiles.

Lean Body Mass, Muscle Architecture, and Performance in Well-Trained Female Weightlifters.

Lean mass and quadriceps muscle architecture have been associated with performance in male well-trained weightlifters, but no data exist for female weightlifters. The aim of the study is to investigate the relationship between lean mass, quadriceps cross sectional area (CSA), and muscle architecture with weightlifting performance in female weightlifters. Eight well-trained female weightlifters (age 23.5 ± 6.3 years, maximum total lifting performance = 147.4 ± 34.1 kg) participated in the study. Five of the athletes were members of the national team and three were among the nation's top-five performers of the respective body-weight category. Measurements included maximum lifting performance in snatch and clean and jerk, body composition (dual x-ray absorptiometry), vastus lateralis (VL) muscle architecture, vastus intermedius (VI) muscle thickness and quadriceps muscles' CSA and countermovement jump (CMJ). Very large to nearly perfect correlations were found between snatch and clean and jerk for trunk lean body mass (r = 0.959 and 0.929), for total CSA (r = 0.732 and 0.608), and CMJ power (r = 0.933 and 0.896). These results suggest that lean body mass, quadriceps' CSA and CMJ should be monitored regularly in female weightlifters to detect potential modifications in lifting performance.

Gastrocnemius Medialis Architectural Properties in Flexibility Trained and Not Trained Child Female Athletes: A Pilot Study.

Gastrocnemius medialis (GM) architecture and ankle angle were compared between flexibility trained (n = 10) and not trained (n = 6) female athletes, aged 8-10 years. Ankle angle, fascicle length, pennation angle and muscle thickness were measured at the mid-belly and the distal part of GM, at rest and at the end of one min of static stretching. Flexibility trained (FT) and not trained athletes (FNT) had similar fascicle length at the medial (4.19 ± 0.37 vs. 4.24 ± 0.54 cm, respectively, p = 0.841) and the distal part of GM (4.25 ± 0.35 vs. 4.18 ± 0.65 cm, respectively, p = 0.780), similar pennation angles, and muscle thickness (p > 0.216), and larger ankle angle at rest (120.9 ± 4.2 vs. 110.9 ± 5.8°, respectively, p = 0.001). During stretching, FT displayed greater fascicle elongation compared to FNT at the medial (+1.67 ± 0.37 vs. +1.28 ± 0.22 cm, respectively, p = 0.048) and the distal part (+1.84 ± 0.67 vs. +0.97 ± 0.97 cm, respectively, p = 0.013), larger change in joint angle and muscle tendon junction displacement (MTJ) (p < 0.001). Muscle thickness was similar in both groups (p > 0.053). Ankle dorsiflexion angle significantly correlated with fascicle elongation at the distal part of GM (r = -0.638, p < 0.01) and MTJ displacement (r = -0.610, p < 0.05). Collectively, FT had greater fascicle elongation at the medial and distal part of GM and greater MTJ displacement during stretching than FNT of similar age.

Muscle fiber composition, jumping performance, and rate of force development adaptations induced by different power training volumes in females.

This study aimed to investigate the effect of 3 different eccentric-only power training volumes on muscle fiber type composition and power performance. Twenty-nine females were assigned into 3 groups and performed 10 weeks of either 3 (low volume), 6 (moderate volume), or 9 (high volume) sets/session of 4 fast-velocity eccentric-only half-squats against 70% of concentric 1-repetition maximum (1RM), followed by 3 maximum countermovement jumps (CMJs) after each set. Half-squat 1RM, CMJ height/power, maximum isometric force, rate of force development (RFD) and muscle fiber cross-sectional area (CSA) were increased in all groups (p = 0.001). Low-volume training induced higher increases in CMJ height/power and early RFD, compared with the moderate- and high-volume training programs (p < 0.001). Significant reductions in type IIx muscle fiber percentages and %CSAs were found after moderate- and high-volume training, with concomitant increases in type IIa fibers (p = 0.001). Significant correlations were found between the changes in type IIa and type IIx percentages, fiber CSA, %CSA, and the changes in performance (r: -0.787 to 0.792; p < 0.05). These results suggest that relatively large eccentric power training volumes may result in detrimental neuromuscular adaptations, minimal changes in early RFD, and a reduction of type IIx muscle fiber percentage. Novelty Low but not high volume of power training maintains type IIx muscle fibers. Early rate of force development increases after a low- or moderate-power training volume, but not after a high-power training volume. Training-induced changes in type IIx muscle fiber percentage is related with changes in early rate of force development.

Changes in Muscle Power and Muscle Morphology with Different Volumes of Fast Eccentric Half-Squats.

The aim of the study was to evaluate power performance and muscle morphology adaptations in response to 5 weeks of fast-eccentric squat training (FEST) performed twice per week, with three different training volumes. Twenty-five moderately trained females were assigned into three groups performing eight repetitions of FEST of either four sets (4 × 8 group; N = 9), 6 sets (6 × 8 group; N = 8) or eight sets (8 × 8 group, N = 8). Before and after the intervention, countermovement jumping height (CMJh) and power (CMJp), half squat maximal strength (1-RM), quadriceps cross-sectional area (QCSA) and vastus lateralis (VL) architecture and fiber type composition were evaluated. Significant increases (p < 0.05) were found for all groups, with no differences among them in 1-RM (4 × 8: 14.8 ± 8.2%, 6 × 8: 13.1 ± 9.2% and 8 × 8: 21.6 ± 7.0%), CMJh (4 × 8: 12.5 ± 8.5%, 6 × 8: 11.3 ± 9.3% and 8 × 8: 7.0 ± 6.2%), CMJp (4 × 8: 9.1 ± 6.0%, 6 × 8: 7.1 ± 5.2% and 8 × 8: 5.0 ± 3.9%) and QCSA (4 × 8: 7.7 ± 4.7%, 6 × 8: 9.0 ± 6.8% and 8 × 8: 8.2 ± 6.5%). Muscle fiber type distribution remained unaltered after training in all groups. VL fascicle length increased and fascicle angle decreased only in 6 × 8 and 8 × 8 groups. In conclusion, four sets of eight fast-eccentric squats/week increase lower body power and strength performance and maintain type IIX muscle fibers after 5 weeks, at least in moderately trained females.