On the Security of a PUF-Based Authentication and Key Exchange Protocol for IoT Devices.

Recently, Roy et al. proposed a physically unclonable function (PUF)-based authentication and key exchange protocol for Internet of Things (IoT) devices. The PUF protocol is efficient, because it integrates both the Node-to-Node (N2N) authentication and the Node-to-Server (N2S) authentication into a standalone protocol. In this paper, we therefore examine the security of the PUF protocol under the assumption of an insider attack. Our cryptanalysis findings are the following. (1) A legitimate but malicious IoT node can monitor the secure communication among the server and any other IoT nodes in both N2N authentication and N2S authentication. (2) A legitimate but malicious IoT node is able to impersonate a target IoT node to cheat the server and any other IoT nodes in N2N authentication and the server in N2S authentication, respectively. (3) A legitimate but malicious IoT node can masquerade as the server to cheat any other target IoT nodes in both N2N authentication and N2S authentication. To the best of our knowledge, our work gives the first non-trivial concrete security analysis for the PUF protocol. In addition, we employ the automatic verification tool of security protocols, i.e., Scyther, to confirm the weaknesses found in the PUF protocol. We finally consider how to prevent weaknesses in the PUF protocol.

Long non-coding RNA ncRuPAR regulates gastric cancer cell proliferation and apoptosis via phosphoinositide 3-kinase/protein kinase B signaling.

Objective: To determine the effect and mechanism of the long non-coding RNA (lncRNA) ncRuPAR (non-protein coding RNA, upstream of coagulation factor II thrombin receptor [F2R]/protease-activated receptor-1 [PAR-1]) in human gastric cancer. Methods: HGC-27-ncRuPAR overexpression and MGC-803-ncRuPAR-RNAi knockdown gastric cancer cell lines were established. We assessed the effect of ncRuPAR on cell proliferation, apoptosis, migration, and invasion using Cell Counting Kit 8, flow cytometry, scratch and transwell assays, respectively. Differentially expressed genes in HGC-27-ncRuPAR overexpression and HGC-27-empty vector cell lines were identified using Affymetrix GeneChip microarray analysis. Ingenuity Pathway Analysis (IPA) of the microarray results was subsequently conducted to identify ncRuPAR-enriched pathways, followed by validation using real time-quantitative PCR (RT-qPCR). As one of the top enriched pathways, phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway was further examined by western blotting to determine its role in ncRuPAR-mediated regulation of gastric cancer pathogenesis. Results: ncRuPAR inhibited human gastric cancer cell proliferation and induced G1/S phase arrest and apoptosis, but did not affect migration or invasion in vitro. Overexpression of ncRuPAR in vitro was found to inhibit its known target PAR-1, as well as PI3K/Akt signaling. The downstream targets of PI3K/Akt, cyclin D1 was downregulated, but there was no change in expression level of B-cell lymphoma 2 (Bcl-2). Conclusions: We showed that lncRNA-ncRuPAR could inhibit tumor cell proliferation and promote apoptosis of human gastric cancer cells, potentially by inhibiting PAR-1, PI3K/Akt signaling, and cyclin D1. The results suggest a potential role for lncRNAs as key regulatory hubs in GC progression.

Research progress in targeted therapies for gastric cancer.

Patients with advanced gastric cancer experience rapid disease progression with limited survival, high mortality, and a lack of surgical options. Thus, radiochemotherapy or a combination of chemotherapeutics with targeted therapy is the mainstay of treatment. In comparison to the treatment of other malignant tumors, in gastric cancer, the development of molecularly targeted drugs has been relatively slow. Currently, there are two major classes of molecularly targeted drug regimens that have achieved a certain efficacy in clinical practice: anti-vascular endothelial growth factor (anti-VEGF) therapy and anti-epidermal growth factor receptor (anti-EGFR) therapy. Trastuzumab has been approved as the standard of care for first-line treatment in advanced human epidermal growth factor receptor 2 (HER2)-positive gastric cancer. Ramucirumab in combination with paclitaxel is the recommended regimen for second-line treatment, and apatinib is recommended as third-line treatment. This review summarizes the current status of targeted therapies in the treatment of gastric cancer and gives a perspective on the future.

A novel fluorescent probe with aggregation induced emission (AIE) effect based on 1,4-dihydropyridine and its applications.

A fluorescent hydrazine hydrate probe (DMA) based on 1,4-dihydropyridine derivatives was designed and synthesized. The fluorescence emission peak of this probe is in the near-infrared region (667 nm), which has good selectivity to hydrazine hydrate and low detection limit (11 nM). Importantly, the probe exhibits aggregation-induced emission (AIE) characteristics. In addition, the probe is prepared with a portable test paper to realize the identification of hydrazine hydrate in the solution and the quantitative detection of hydrazine hydrate gas.

Xiaotansanjiefang inhibits the viability of colorectal cancer cells via Jagged 1/Notch 3/Snail signaling pathway.

The purpose of this study is to explore the anti-colorectal cancer of Xiaotansanjiefang, a famous traditional Chinese medicine, and its potential anti-cancer mechanism. In this study, the HCT116 cell spheres were prepared as in vitro study model. We found the Xiaotansanjiefang medication was able to inhibit the proliferation of HCT116 cell spheres in a dose-dependent manner, especially in 3 and 6 mg/ml Xiaotansanjiefang medication treated groups. We also found the high concentration of Xiaotansanjiefang medication could suppress the migration and promote the apoptosis of HCT116 cell spheres. Moreover, we found the expression of Jagged 1, Notch 3, Snail, and Hes 1 were decreased in HCT116 cell spheres treated with Xiaotansanjiefang medication. Furthermore, the proliferation and apoptosis behaviors of HCT116 cell spheres treated with Xiaotansanjiefang medication were reversed with the addition of Jagged 1 Fc chimera protein. The expression of Jagged 1, Notch 3, Snail, and Hes 1 were also increased again in HCT116 cells treated with Xiaotansanjiefang medication plus with Jagged 1 Fc chimera protein. The presented study may provide a promising strategy to treat and prevent colorectal cancer.

Curcumin Alleviates Oxaliplatin-Induced Peripheral Neuropathic Pain through Inhibiting Oxidative Stress-Mediated Activation of NF-κB and Mitigating Inflammation.

Oxaliplatin is a first-line clinical drug in cancer treatment and its side effects of peripheral neuropathic pain have also attracted much attention. Neuroinflammation induced by oxidative stress-mediated activation of nuclear factor-kappa B (NF-κB) plays an important role in the course. Current studies have shown that curcumin has various biological activities like antioxidant, anti-inflammatory, antitumor and so on, while few studies were conducted about its role in oxaliplatin-induced peripheral neuropathic pain. The aim of this study is to verify the mechanism of curcumin alleviating oxaliplatin-induced peripheral neuropathic pain. Intraperitoneal injection with oxaliplatin (4 mg/kg body weight) was given to the rats twice a week and last for four weeks to establish the model rats. Gavage administration of curcumin (12.5, 25, and 50 mg/kg body weight, respectively) was conducted for consecutive 28 d to explore the effects and potential mechanism. Our results showed that curcumin administration could increase mechanical withdrawal threshold and decrease the paw-withdrawal times of cold allodynia significantly; meanwhile, motor nerve conduction velocity (MNCV) and sense nerve conduction velocity (SNCV) were both increased and the injured neurons of the spinal cord were repaired. In addition, curcumin administration increased superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and reduced malondialdehyde (MDA). Moreover, the curcumin operation inhibited the activated of NF-κB and level of inflammatory factors like tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß) and interleukin-6 (IL-6). In conclusion, these findings suggested that curcumin could alleviate oxaliplatin-induced peripheral neuropathic pain; the mechanism might be inhibiting oxidative stress-mediated activation of NF-κB and mitigating neuroinflammation.

On Secure Simple Pairing in Bluetooth Standard v5.0-Part I: Authenticated Link Key Security and Its Home Automation and Entertainment Applications.

Bluetooth is an important technical standard for short-range and low-power wireless communication. The home automation and entertainment (HAE) systems often make use of Bluetooth technology to link different Bluetooth devices and form Bluetooth networks. The security concerns of the HAE systems are raised due to massive deployment of the Bluetooth devices. The Bluetooth standard mainly depends on the secure simple pairing (SSP) solution to protect the Bluetooth devices. Hence, we investigate the SSP solution according to the Bluetooth standard v5.0. The contributions are threefold. (1) A formal security model is proposed to evaluate SSP's association models and authenticated link key. (2) We formally analyze two SSP protocols and present the security requirements for basic cryptographic modules in these SSP protocols. (3) We discuss the typical SSP applications in the HAE systems. Our results are useful to not only evaluating and designing the SSP protocols but also enhancing the security of the HAE systems in which the Bluetooth access is available.

On Secure Simple Pairing in Bluetooth Standard v5.0-Part II: Privacy Analysis and Enhancement for Low Energy.

Bluetooth low energy devices are very popular in wireless personal area networks. According to the Bluetooth standard specifications, the low energy secure simple pairing (LESSP) protocol is the process by which the pairing devices negotiate the authenticated secret key. To violate the user privacy, the adversary can perhaps link the runs of the LESSP protocol to the targeted device, which usually relates to the specially appointed user. Hence, we investigate deep into the privacy of the LESSP protocol. Our main contributions are threefold: (1) We demonstrate that the LESSP protocol suffers from privacy vulnerability. That is, an adversary without any secret key is able to identify the targeted device by the LESSP protocol. (2) An improvement is therefore proposed to repair the privacy vulnerability in the LESSP protocol. (3) We develop a formal privacy model to evaluate the privacy vulnerabilities in the LESSP protocol and its improved versions. We further prove that our improvement on the LESSP protocol is private under the privacy model. In addition, the performance evaluation shows that our improvement is as efficient as the LESSP protocol. Our research results are beneficial to the privacy enhancement of Bluetooth systems in wireless personal area networks.

[Inhibitory effect of Xiaotan Sanjie Recipe on the microsatellite instability of orthotopic transplantation tumor in MKN-45 human gastric cancer nude mice].

OBJECTIVE: To study the inhibitory effect of Xiaotan Sanjie Recipe (XSR) on the microsatellite instability of orthotopic transplantation tumor in MKN-45 human gastric cancer nude mice. METHODS: The 3rd passage subcutaneous transplantation tumor was taken as the origin of the model by using MKN-45 human gastric cancer cell lines. MKN-45 human gastric cancer nude mouse model was established using OB glue adhesive method. Then 30 nude mice were divided into the model group, the XSR group, and the chemotherapy group. Mice in the XSR group were intragastrically given XSR at the daily dose of 0.4 mL. Mice in the chemotherapy group were intragastrically given Fluorouracil at the daily dose of 0.4 mL. No intervention was given to mice in the model group. After 6 weeks of medication, the tumor weight was measured, and the tumor inhibition rate calculated. The size, the peak height, and the peak area of 5 microsatellite instability sites were detected. RESULTS: The tumor inhibition rate was 40. 84% in the XSR group. The tumor weight was significantly lower in the XSR group than in the model group (P < 0.01), showing no statistical difference when compared with the chemotherapy group (P >0.05). The incidence of high microsatellite instability (MSI-H) in the model group was 70%, and the incidence of low microsatellite instability (MSI-L) was 30%. Microsatellite stable site tended be stable after 6 weeks of XSR treatment. CONCLUSION: XSR showed inhibition on microsatellite instable orthotopic transplantation tumor in MKN-45 human gastric cancer nude mice.

DNA-functionalized quantum dots: fabrication, structural, and physicochemical properties.

We have systematically investigated the effect of physicochemical conditions, such as pH, salt concentration, and DNA/nanoparticle ratio, on the chemical conjugation process and structural and optical stability of carboxyl-functionalized quantum dots (QDs) functionalized with amino-modified DNA. We reveal the relationship between aqueous conditions and the amount of DNA conjugated on QDs, colloidal stability, and yield of the final QD-DNA conjugates. By carefully adjusting the environmental variables we have successfully achieved up to 20 DNA strands conjugated per QD, and demonstrated how this number can be tuned. The fabricated QD-DNA conjugates are dispersed and optically stable in salted solutions for over a month. We have also evaluated the involved interparticle interactions to explain the solution behavior of QD-DNA conjugates. Our results provide a basic understanding of the physiochemical processes governing a nanoparticle-biomolecule conjugation and the structural stability of the formed conjugates. Such fabricated QD-DNA conjugates might be of great benefit for programmable assemblies of optically active nanomaterials and for emerging biosensing methods based on nanomaterials.

Xiaotan Sanjie decoction normalizes tumor permissive microenvironment in gastric cancer (Review).

Gastric cancer (GC) develops in a complex tissue environment, the tumor microenvironment (TME), which it relies on for persistent proliferation, migration, invasion and metastasis. Non­malignant stromal cell types within the TME are regarded as a clinical meaningful target with the lower risk of resistance and tumor relapse. Studies have revealed that the Xiaotan Sanjie decoction, which is formulated on the basis of the theory of phlegm syndrome, a Traditional Chinese Medicine concept, modulates released factors such as transforming growth factor­ß from tumor cells, immune cells, cancer­associated fibroblasts, extracellular matrix, as well as vascular endothelial growth factor involved in the process of angiogenesis within the TME. Clinical studies have also shown that the Xiaotan Sanjie decoction is associated with favorable survival and quality of life. The present review aimed to interpret the hypothesis that Xiaotan Sanjie decoction has the ability to normalize the GC tumor cells by influencing functions of stromal cells within the TME. The possible association between phlegm syndrome and the TME in GC was discussed in the present review. Overall, Xiaotan Sanjie decoction may be suitable to be added to tumor cell­directed agents or emerging immunotherapies becoming a desirable modality in the management of GC and acquire improved outcomes for patients with GC.

Phase Change Material Enhanced Radiative Cooler for Temperature-Adaptive Thermal Regulation.

Passive radiative cooling (PRC), as an electricity-free and environmentally friendly cooling strategy, is highly desirable in improving the global energy landscape. Despite numerous efforts, most designs for PRC are so devoted to improving the cooling performance in the daytime that they neglect the triggered overcooling at night. Herein, we approached an effective design for temperature-adaptive thermal management through integrating PRC and temperature control of room-temperature phase change material. Compared with conventional radiative coolers, the developed phase change material-enhanced radiative cooler (PCMRC) can adjust its performance according to the temperature of day and night. The PCMRC achieved an average subambient temperature drop of ∼6.3 °C under direct sunlight and an average temperature rise of ∼2.1 °C above ambient temperature at night, as well as a reduced temperature difference between day and night. The temperature-adaptive PCMRC shows great promise for passive radiative cooling regulation, which can further extend the applications of passive radiative cooling.

ZIF-67-modified magnetic nanoparticles for extraction of phenoxy carboxylic acid herbicides.

Phenoxy carboxylic acid (PCA) herbicides are commonly used herbicides that can easily accumulate in soil, groundwater, crops, and vegetable surfaces. Thus, they pose a serious risk to human health. Accurate detection of trace amounts of PCAs in various matrixes is crucial. Herein, ZIF-67-modified magnetic nanoparticles (MNPs, ZIF-67@Fe3O4) were prepared by growing ZIF-67 on the surface of Fe3O4 MNPs. The introduction of ZIF-67 improved the dispersion of Fe3O4 nanoparticles in water and enhanced their extraction performance for PCAs. When an eluent consisting of ammonia water and acetonitrile (5% : 95%; v/v) was employed, 10 mg of ZIF-67@Fe3O4 displayed optimal extraction performance for PCAs in a 20 mL sample solution at a pH of 3. We achieved a limit of detection ranging from 0.014 µg L-1 to 0.056 µg L-1 for four types of PCA herbicides by using the newly developed method. Notably, the values were considerably lower than the maximum concentration levels of PCAs in drinking water set by the Environmental Protection Agency. The relative recovery rate of PCAs using ZIF-67@Fe3O4 ranged from 83.75% to 117.07% when applied to river water and apple samples. These results demonstrate the great potential of ZIF-67@Fe3O4 in determining the residues of organic pesticides in real samples.

Study on the flocculation mechanism of a bioflocculant from Acinetobacter sp. TH40 combined with graphene oxide for the removal of metal ions from water.

In order to realize the simultaneous removal of nine metal ions from water, in this study, an excellent flocculant suitable for the simultaneous removal of multiple metal ions in water was developed by using the excellent flocculation properties of graphene oxide (GO) combined with biological flocculants. First, this study investigated the concentrations and pollution levels of nine metal pollutants in surface water and groundwater of a typical city in central China. The maximum concentrations of these nine metal ions were Al 0.29, Ni 0.0325, Ba 0.948, Fe 1.12, As 0.05, Cd 0.01, Zn 1.45, Mn 1.24, and Hg 0.16 (in mg/L). Second, the three-dimensional structure diagram of GO was established. Gaussian16W software and the pm6D3 semi-empirical method were used to analysis the structure and the vibration of GO. The B3LYP function and basis set DEF2SVP was used to calculate the single point energy. Third, with varying the flocculation time, it was found that the maximum flocculation efficiency could reach more than 80.00% under the optimal conditions, that is, with a metal ion mixture of 20 mg/L. The optimal dosage of GO was 15 mg/L. The optimal time for bioflocculation efficiency was 2.5 h, and the optimal concentration of bioflocculant was 3 mg/L. The optimal flocculation efficiency was 82.01% under the optimal conditions.

A chinese herbal formula to improve general psychological status in posttraumatic stress disorder: a randomized placebo-controlled trial on sichuan earthquake survivors.

Introduction. Posttraumatic stress disorder (PTSD) is accompanied by poor general psychological status (GPS). In the present study, we investigated the effects of a Chinese herbal formula on GPS in earthquake survivors with PTSD. Methods. A randomized, double-blind, placebo-controlled trial compared a Chinese herbal formula, Xiao-Tan-Jie-Yu-Fang (XTJYF), to placebo in 2008 Sichuan earthquake survivors with PTSD. Patients were randomized into XTJYF (n = 123) and placebo (n = 122) groups. Baseline-to-end-point score changes in the three global indices of the Symptom Checklist-90-Revised (SCL-90-R) and rates of response in the SCL global severity index (GSI) were the primary endpoints. A subanalysis of the nine SCL factors and the sleep quality score were secondary endpoints. Results and Discussion. Compared to placebo, the XTJYF group was significantly improved in all three SCL global indices (P = 0.001~0.028). More patients in the XTJYF group reported "much improved" than the placebo group (P = 0.001). The XTJYF group performed significantly better than control in five out of nine SCL factors (somatization, obsessive-compulsive behavior, depression, anxiety, and hostility (P = 0.001~0.036)), and in sleep quality score (P < 0.001). XTJYF produced no serious adverse events. These findings suggest that XTJYF may be an effective and safe treatment option for improving GPS in patients with PTSD.

Ziyin Huatan Recipe, a Chinese herbal compound, inhibits migration and invasion of gastric cancer by upregulating RUNX3 expression.

OBJECTIVES: Ziyin Huatan Recipe (ZYHT), a traditional Chinese medicine comprised of Lilii Bulbus, Pinelliae Rhizoma, and Hedyotis Diffusa, has shown promise in treating gastric cancer (GC). However, its potential mechanism has not yet been clearly addressed. This study aimed to predict targets and molecular mechanisms of ZYHT in treating GC by network pharmacology analysis and to explore the role of ZYHT in GC both in vitro and in vivo. METHODS: Targets and molecular mechanisms of ZYHT were predicted via network pharmacology analysis. The effects of ZYHT on the expression of metastasis-associated targets were further validated by Western blot and quantitative real-time polymerase chain reaction. To explore the specific molecular mechanisms of the effects of ZYHT on migration and invasion, the runt-related transcription factor 3 (RUNX3) gene was knocked out by clustered regularly interspaced short palindromic repeats/Cas9, and lentiviral vectors were transfected into SGC-7901 cells. Then lung metastasis model of GC in nude mice was established to explore the anti-metastasis effect of ZYHT. Western blot and immunohistochemistry were used to explore the impact of ZYHT on the expression of metastasis-related proteins with or without RUNX3 gene. RESULTS: The network pharmacology analysis showed that ZYHT might inhibit focal adhesion, migration, invasion and metastasis of GC. ZYHT inhibited the proliferation, migration and invasion of GC cells in vitro via regulating the expression of metastasis-associated targets. Knocking out RUNX3 almost completely reversed the cell phenotypes (migration and invasion) and protein expression levels elicited by ZYHT. In vivo studies showed that ZYHT inhibited the metastasis of GC cells to the lung and prolonged the survival time of the nude mice. Knocking out RUNX3 partly reversed the metastasis of GC cells to the lung and the protein expression levels elicited by ZYHT. CONCLUSION: ZYHT can effectively inhibit the invasion and migration of GC in vitro and in vivo, and its molecular mechanism may relate to the upregulation of RUNX3 expression.

Poly(lactic acid)/artificially cultured diatom frustules nanofibrous membranes with fast and controllable degradation rates for air filtration.

The worldwide pandemic, coronavirus COVID-19, has been posing a serious threat to the global economy and security in last 2 years. The monthly consumption and subsequent discarding of 129 billion masks (equivalent to 645,000 tons) pose a serious detrimental impact on environmental sustainability. In this study, we report a novel type of nanofibrous membranes (NFMs) with supreme filtration performance and controllable degradation rates, which are mainly composed of polylactic acid (PLA) and artificially cultured diatom frustules (DFs). In this way, the filtration efficiency of particular matter (PM) and the pressure drop were significantly improved in the prepared PLA/DFs NFMs as compared with the neat PLA NFM. In specific, with incorporation of 5% DFs into fibers, PM0.3 removal with a filtration efficiency of over 99% and a pressure drop of 109 Pa were achieved with a membrane thickness of only 0.1 mm. Moreover, the yield strength and crystallinity degree of the PLA/DFs5 NFMs were sharply increased from 1.88 Mpa and 26.37% to 2.72 Mpa and 30.02%. Besides those unique characters, the PLA/DFs5 presented excellent degradability, accompanied by the degradation of 38% in 0.01 M sodium hydroxide solution after 7 days and approximately 100% in natural condition after 42 days, respectively. Meanwhile, the environmentally friendly raw materials of the composite polylactic acid and artificially cultured diatom frustules could be extracted from corn-derived biomass and artificially cultivated diatoms, ensuring the conformance to carbon neutrality and promising applications in personal protection. Supplementary information: The online version contains supplementary material available at 10.1007/s42114-022-00474-7.

Adhesive hydrogel wrap loaded with Netrin-1-modified adipose-derived stem cells: An effective approach against periarterial inflammation after endovascular intervention.

Endovascular interventions, such as balloon dilation and stent implantation, are currently recommended as the primary treatment for patients with peripheral artery disease (PAD), greatly improving patient prognosis. However, the consequent lumen restenosis that occurs after endovascular interventions has become an important clinical problem. Inflammation has been proven to be crucial to postoperative restenosis. In previous studies we have identified that Netrin-1-modified adipose-derived stem cells (N-ADSCs) transplantation is an effective anti-inflammatory strategy to repair vascular damage. Nevertheless, it remained to be explored how one could constantly deliver N-ADSCs onto damaged arteries. Therefore, we developed an adhesive double network (DN) hydrogel wrap loaded with N-ADSCs for sustained perivascular delivery. Inspired by the adhesion mechanism of mussels, we developed an adhesive and tough polyacrylamide/calcium-alginate/reduced graphene oxide/polydopamine (PAM/CA/rGO/PDA) hydrogel. Dopamine was attached to graphene sheets and limitedly oxidized to generate free catechol groups. The hydrogel could wrap damaged arteries and induce anti-inflammatory effects through N-ADSCs. In vitro experiments demonstrated that N-ADSCs significantly promoted the M2 polarization of macrophages to anti-inflammatory phenotypes and reduced the expression of inflammatory factors. In vivo experiments in a rat carotid artery guidewire injury model showed that the adhesive hydrogel wrap loaded with N-ADSCs could significantly reduce arterial inflammation, inhibit intimal hyperplasia and improve re-endothelialization. Altogether, this newly developed N-ADSCs-loaded hydrogel wrap provides an effective slow-releasing system, which may be a promising way to prevent and treat restenosis after endovascular interventions.

Conductive Hydrogel Conduits with Growth Factor Gradients for Peripheral Nerve Repair in Diabetics with Non-Suture Tape.

Diabetic patients suffer from peripheral nerve injury with slow and incomplete regeneration owing to hyperglycemia and microvascular complications. This study develops a graphene-based nerve guidance conduit by incorporating natural double network hydrogel and a neurotrophic concentration gradient with non-invasive treatment for diabetics. GelMA/silk fibroin double network hydrogel plays quadruple roles for rapid setting/curing, suitable mechanical supporting, good biocompatibility, and sustainable growth factor delivery. Meanwhile, graphene mesh can improve the toughness of conduit and enhance conductivity of conduit for regeneration. Here, novel silk tapes show quick and tough adhesion of wet tissue by dual mechanism to replace suture step. The in vivo results demonstrate that gradient concentration of netrin-1 in conduit have better performance than uniform concentration caused by chemotaxis phenomenon for axon extension, remyelination, and angiogenesis. Altogether, GelMA/silk graphene conduit with gradient netrin-1 and dry double-sided adhesive tape can significantly promote repairing of peripheral nerve injury and inhibit the atrophy of muscles for diabetics.

Binary heterogeneous superlattices assembled from quantum dots and gold nanoparticles with DNA.

Controllable assembly of three-dimensional (3D) superlattices composed of different types of nanoscale objects opens new opportunities for material fabrication. Herein we show the successful assembly of heterogeneous 3D structures from gold nanoparticles (AuNPs) and quantum dots (QDs) using DNA encoding. By applying synchrotron-based small-angle X-ray scattering, we found that AuNPs and QDs are positioned in a body-centered cubic lattice, while each particle type, AuNP and QD, is arranged in a simple-cubic manner. Our studies demonstrate a route for assembly of integrated heterogeneous 3D structures from different nano-objects by DNA-encoded interactions.