Diversity-Oriented One-Pot Synthesis to Construct Functionalized Chroman-2-one Derivatives and Other Heterocyclic Compounds.

The asymmetric organocatalyzed diversity-oriented one-pot synthesis has been developed to construct chroman-2-one derivatives and other heterocyclic compounds with excellent efficiency and stereoselectivity. The reactions represent a challenging issue, since it altered the inherent selectivity profiles exhibited by the substrates of 2-hydroxycinnamaldehyde 1 and trans-ß-nitrostyrene 2, which was previously reported as the asymmetric oxa-Michael-Michael cascade to generate chiral chromans. It should be noted that polycyclic O,O-acetal-containing compounds, which are found in numerous natural products and biologically interesting molecules, could also be achieved in good yields with excellent enantioselectivity as a single diastereoisomer with five continuous stereogenic centers.

Do some anxiety disorders belong to the prodrome of bipolar disorder? A clinical study combining retrospective and prospective methods to analyse the relationship between anxiety disorder and bipolar disorder from the perspective of biorhythms.

BACKGROUND: In clinical practice, some patients diagnosed with anxiety disorder (AD) may develop bipolar disorder (BD) many years later, and some cases of AD may be cured by the use of mood stabilizers. However, the relationship between AD and BD should be explored further. METHOD: To track how many cases of AD turned to BD and to discover the differences between them, we recruited 48 patients diagnosed with BD, who were assigned to the BD group for the retrospective analysis, and we also recruited 186 patients diagnosed with AD at enrolment; this latter group was asked to complete follow-up surveys conducted 3 months, 6 months, 12 months and 18 months after the primary stage of the study. We defined another two groups according to the usage of mood stabilizers, the rates of reduction in scores on the Hamilton Anxiety Scale and Yale-Brown Obsessive Compulsive Scale, and the changes in Clinical Global Impression scores at different follow-up times: the anxiety group and the atypical BD group (who used mood stabilizers to treat AD). All subjects also completed the NEO Five-Factor Inventory and supplied blood samples to be tested for several endocrine indices (TSH, T3, FT3, T4, FT4, ACTH,PTC) and inflammatory cytokines (IL-6, IL-8, IL-10, TNF-α, CRP) at enrolment. RESULTS: In total, 14 subjects developed BD by the end of the study. One hundred eleven subjects were included in the anxiety group. Sixty-three subjects were assigned to the atypical BD group, and they had similar features to the 48 subjects in the BD group in terms of personality traits, abnormality rates of endocrine indices and levels of inflammatory cytokines. From the anxiety group to the atypical BD group and then the BD group, the age of first onset gradually decreased, while the frequency of onset and the score of suicidal ideation gradually increased. Furthermore, the atypical BD group showed markedly higher levels of TSH, IL-6, TNF-α and CRP than the other two groups. CONCLUSIONS: Some ADs with unique features might belong to the prodromal stage or the atypical presentation of BD, and recognizing these ADs early will economize many medical resources.

[Role of HPA and HPT Axis in Anxiety Disorder Complicated with Diabetes Mellitus].

OBJECTIVE: To determine the mediating role of hypothalamic-pituitary-adrenal axis (HPA) and hypothalamic-pituitary-thyroid axis (HPT) axis in anxiety disorder in patients with diabetes mellitus. METHODS: A total of 562 hospitalized patients with anxiety disorder participate in the study. Serum thyroid-stimulating hormone (TSH),total triiodothyronine (TT3),free triiodothyronine (FT3),total tetraiodothyronine (TT4),free tetraiodothyronine (FT4) ,adrenocorticotropic hormone (ACTH) and cortisol (PTC) were measured. Glucose tolerance test (OGTT) was performed,estimating insulin resistance index (HOMA IR) and insulin sensitivity index (WBISI). RESULTS: Of the participants,83 (14.8%) had diabetes. In those who were younger than 40 yr.,the diabetic patients were more likely to have abnormal FT4 and HPT ( P<0.05). The patients with diabetes were more likely to be older (OR=1.067, 95%CI:1.041-1.094, P=0.000) and have higher FT4 (OR=1.104, 95%CI:1.022-1.193, P=0.012) and PTC (OR=1.001, 95%CI:1.000-1.003, P=0.025) . Insulin resistance index increased while insulin sensitivity index decreased ( P<0.05) with abnormal PTC and HPA axis. Insulin sensitivity index decreased ( P<0.05) when ACTH,HPT axis,FT4 and TT3 were abnormal. CONCLUSION: Abnormal HPA or HPT axis mediates diabetic complications in patients with anxiety disorder. Early interventions on neuroendocrine hormone abnormality may help prevent diabetes in patients with anxiety disorder.

[Study the effects of neuroplasticity on major depression disorder in rTMS combined with antidepressant treatments].

OBJECTIVE: To explore the effects of neuroplasticity on major depression disorder (MDD) with event related potentials (ERPs) of mismatch negativity (MMN) and sensory gating potentials P50 for repetitive transcranial magnetic stimulation (rTMS) combined with antidepressant treatments. METHODS: A total of 159 patients with MDD randomly divided into two groups: 75 patients in group A was administrated rTMS treatment for 2 weeks (15 days), and continues to give antidepressant of selective serotonin reuptake inhibitor (SSRI) regularly. 84 patients in group B was consistently administered the similar antidepressant. The 24-item Hamilton Depression Scale (HAMD-24), MMN latency, S1-P50 amplitude, S2-P50 amplitude and S2-P50/S1-P50 amplitude ratio, and the percentages of abnormal P50 (S2-P50/S1-P50 > or = 0.5) were assessed and measured before treatment, at the 2nd and the 10th weekend after treatment in two treatment groups. Meanwhile, 90 normal control cases was set up as control group (group C). Results At 2th and 10th week after treatment, the HAMD-24 scores were reduced remarkably than those before treatment in group A and B (P < 0.001), and the scores in group A was lower than that in group B (P < 0.001). MMN latencies and S-P50 amplitudes after treatment in group A were significant shorter and lower than those before treatment in group A and after treatment in group B (P < 0.05) (except for intergroup comparison of S2-P50, amplitude after treatment weeks). No significant difference was found in S2-P50/S1-P50 ratio ( except for after treatment 2 weeks ) and percentage of abnormal P50 between group A after treatment and group C (P > 0.05). There were no statistical significance difference of ERPs between after treatment 2 weeks and 10 weeks in group A (P > 0.05). No statistical differences of ERPs between before and after treatment in group B (P > 0.05) was observed. CONCLUSION: rTMS combined with antidepressant therapy was superior to single antidepressant treatment, and marked improved automatic processing and sensory gating of the brain senior functional electrical physiological index. rTMS might induce effects of long-term potentiation/long-term depression like plasticity on brain, and keep brain function in a dynamic stability and balance. MMN and P50 maybe become neuroplasticity index reflecting relevant the brain senior function.

[Comparative Energy Consumption Structure and Mode between China and Major Energy-Consuming Countries Under the Background of Carbon Emission Reduction].

The top 23 countries with energy consumption accounting for 80% of the worldwide total in 2020 were selected as the research objects for this study. The energy consumption structure of these countries has been compared and studied from the proportion of clean energy in primary energy, the clean utilization rate of fossil energy, and the proportion of electric energy in terminal energy consumption, and the characteristics of energy consumption intensity were also analyzed based on the energy consumption per unit GDP, capital, and unit land area. The energy consumption patterns of various countries in social-economic operation, production, and life were also discussed, combined with the characteristics of industrial structure and subsector energy consumption. The concept of natural carbon sink load ratio of energy consumption was put forward, and the advantages and challenges of energy consumption mode transformation under carbon peak and carbon neutralization goals in China were identified and analyzed. The results showed that:the proportion of clean energy in primary energy, the proportion of energy consumption for power generation, the clean utilization rate of fossil energy, and the proportion of electric energy in terminal energy consumption in China were 15.90%, 53.48%, 37.51%, and 26.54%,respectively,all of which were in the forefront among the major energy-consuming countries in the world. China has built a favorable structural foundation for energy-intensive and clean utilization and formed a green and low-carbon energy consumption model; the proportion of non-industrial and agricultural energy consumption, especially that of transportation energy consumption, were both the lowest among major energy-consuming countries, and there is still much room for improvement in overall energy productivity based on the optimization of industrial structure. However, the relatively short carbon peak and neutralization target period poses a great challenge to the development speed and scale of clean energy to China, and the international situation under the constraint of carbon emission also increases the difficulty for China to achieve the carbon reduction goal by optimizing and adjusting the industrial structure.

Functional Characterization of the Ryanodine Receptor Gene in Diaphorina citri.

The Asian citrus psyllid Diaphorina citri (Hemiptera: Liviidae) is a major citrus pest spread around the world. It is also a vector of the bacterium 'Candidatus Liberibacter asiaticus', considered the cause of the fatal citrus disease huanglongbing (HLB). Insect ryanodine receptors (RyRs) are the primary target sites of diamide insecticides. In this study, full-length RyR cDNA from D. citri (named DcRyR) was isolated and identified. The 15,393 bp long open reading frame of DcRyR encoded a 5130 amino acid protein with a calculated molecular weight of 580,830 kDa. This protein had a high sequence identity (76-79%) with other insect homologs and a low sequence identity (43-46%) with mammals. An MIR domain, two RIH domains, three SPRY domains, four RyR repeat domains, an RIH-associated domain at the N-terminus, two consensus calcium-binding EF-hands, and six transmembrane domains were among the characteristics that DcRyR shared with insect and vertebrate RyRs. In expression analysis, the DcRyR gene displayed transcript abundance in all tissues and developmental stages as well as gene-differential and stage-specific patterns. In addition, diagnostic PCR experiments revealed that DcRyR had three potential alternative splice variants and that splicing events might have contributed to the various functions of DcRyR. However, diamide resistance-related amino acid residue mutations I4790M/K and G4946E were not found in DcRyR. These results can serve as the basis for further investigation into the target-based diamide pesticide resistance of D. citri.

Cognitive and neural correlates of depression-like behaviour in socially defeated mice: an animal model of depression with cognitive dysfunction.

Human depression is associated with cognitive deficits. It is critical to have valid animal models in order to investigate mechanisms and treatment strategies for these associated conditions. The goal of this study was to determine the association of cognitive dysfunction with depression-like behaviour in an animal model of depression and investigate the neural circuits underlying the behaviour. Mice that were exposed to social defeat for 14 d developed depression-like behaviour, i.e. anhedonia and social avoidance as indicated by reduced sucrose preference and decreased social interaction. The assessment of cognitive performance of defeated mice demonstrated impaired working memory in the T-maze continuous alternation task and enhanced fear memory in the contextual and cued fear-conditioning tests. In contrast, reference learning and memory in the Morris water maze test were intact in defeated mice. Neuronal activation following chronic social defeat was investigated by c-fosin-situ hybridization. Defeated mice exhibited preferential neural activity in the prefrontal cortex, cingulate cortex, hippocampal formation, septum, amygdala, and hypothalamic nuclei. Taken together, our results suggest that the chronic social defeat mouse model could serve as a valid animal model to study depression with cognitive impairments. The patterns of neuronal activation provide a neural basis for social defeat-induced changes in behaviour.

Processed Multiparameter Electroencephalogram-Guided General Anesthesia Management Can Reduce Postoperative Delirium Following Carotid Endarterectomy: A Randomized Clinical Trial.

Background: Patients undergoing carotid endarterectomy (CEA) for severe carotid stenosis are vulnerable to postoperative delirium, a complication frequently associated with poor outcome. This study investigated the impact of processed electroencephalogram (EEG)-guided anesthesia management on the incidence of postoperative delirium in patients undergoing CEA. Methods: This single-center, prospective, randomized clinical trial on 255 patients receiving CEA under general anesthesia compared the outcomes of patient state index (PSI) monitoring [SEDLine Brain Function Monitor (Masimo, Inc, Irvine, CA)] (standard group, n = 128) with PSI combined with density spectral array(DSA) -guided monitoring (intervention group, n = 127) to reduce the risk of intraoperative EEG burst suppression. All patients were monitored by continuous transcranial Doppler ultrasound (TCD) and near-infrared spectroscopy (NIRS) to avoid perioperative cerebral hypoperfusion or hyperperfusion. According to the surgical process, EEG suppression time was calculated separately for three stages: S1 (from anesthesia induction to carotid artery clamping), S2 (from clamping to declamping), and S3 (from declamping to the end of surgery). The primary outcome was incidence of postoperative delirium according to the Confusion Assessment Method algorithm during the first 3 days post-surgery, and secondary outcomes were other neurologic complications and length of hospital stay. Results: There were no episodes of cerebral hypoperfusion or hyperperfusion according to TCD and NIRS monitoring in either group during surgery. The incidence of postoperative delirium within 3 days post-surgery was significantly lower in the intervention group than the standard group (7.87 vs. 28.91%, P < 0.01). In the intervention group, the total EEG suppression time and the EEG suppression time during S2 and S3 were shorter (Total, 0 "0" vs. 0 "1.17" min, P = 0.04; S2, 0 "0" vs. 0 "0.1" min, P < 0.01; S3, 0 "0" vs. 0 "0" min, P = 0.02). There were no group differences in incidence of neurologic complications and length of postoperative hospital stay. Conclusion: Processed electroencephalogram-guided general anesthesia management, consisting of PSI combined with DSA monitoring, can significantly reduce the risk of postoperative delirium in patients undergoing CEA. Patients, especially those exhibiting hemodynamic fluctuations or receiving surgical procedures that disrupt cerebral perfusion, may benefit from the monitoring of multiple EEG parameters during surgery. Clinical Trial Registration: www.ClinicalTrials.gov, identifier: NCT03622515.

[A comparative study on behaviors of two depression models in rats induced by chronic forced swimming stress].

OBJECTIVE: To compare the behaviors of rats with depressions induced by chronic forced swimming stress under two different conditions. METHODS: Eighteen male rats were randomly divided into 3 groups, with 6 rats in each group. The rats in the control group (C group) were not forced into swimming, while the rats in the stress groups (S1 and S2) were forced to swim for 14 consecutive days. The rats in S1 group and S2 group swam for five minutes every morning, in water with (23 +/- 1) degree C, and (10 +/- 0.5) degree C in temperature, respectively. The weight gain, food intake, open-field test and saccharin solution test were observed on the seventh day and fourteenth day. RESULTS: On the seventh day following chronic swim stress, the rats in the S2 group had significant lower ratio in weight gain and food intake than the controls (P < 0.05). On the fourteenth day, the rats in the S2 group had significant lower ratio in weight gain (12.26 +/- 4.04)%, food intake (9.49 +/- 0.96)%, sucrose intake (28.63 +/- 3.51) g, and preference for saccharin solution (76.25 +/- 2.51)%, and less number of crossing (12.17 +/- 9.00) and times of rearing (3.17 +/- 3.60) than the controls (P < 0.05). The rats in the S1 group had significant lower ratio in weight gain and food intake than the controls on the seventh day following forced swimming. On the fourteenth day, the rats in the S1 group still had lower ratio in weight gain, but had higher ratio in food intake and preference for saccharin solution, and greater number of crossing than the controls. CONCLUSION: Chronic forced swimming at a lower temperature could induce depression better than at a higher temperature.

[Comparative Analysis of NOx Emission Characteristics of Non-Ultra-Low- and Ultra-Low-Emission Coal-Fired Power Units During the Start-Up Process].

Based on the NOx emission information of 671 coal-fired power units, the NOx emission characteristics of non-ultra-low-emission (non-ULE) and ultra-low-emission (ULE) coal-fired power units during the start-up process were compared and analyzed. The results showed that in the start-up stage of non-ULE units, the exceeding standard rate (ESR) of NOx emission concentration was 81.53%, the average exceeding standard hours (ESHs) was 2.64 h, and the average maximum emission hourly mean concentration (MEHC) was 284.06 mg·m-3. The NOx ESR of ULE units was 79.86%, the average ESHs was 2.52 h, and the average MEHC was 231.61 mg·m-3. There was no significant difference in the ESR and the average ESHs between non-ULE and ULE units, but the difference in the average MEHC was significant. There was no significant difference in the ESR, average ESHs, and average MEHC between different grades of non-ULE units, except that the average MEHC of 300 MW units was significantly lower than that of 200 MW units. There was statistical difference in the ESR, average ESHs, and average MEHC among different grades of ULE units, and 600 MW units performed best in the ESHs control, whereas 1000 MW units performed best in the emission concentration control.

[Association of serotonin and norepinephrine transporter gene polymorphisms with the susceptibility to depression].

OBJECTIVE: To determine whether the serotonin transporter (5-HTT) and norepinephrine transporter (NET) gene polymorphisms were associated with the susceptibility to depression. METHODS: Five hundred and seventy-nine patients with depression, evaluated using a 17-item Hamilton Depression Rating Scale for Depression (HAMD), and 437 healthy controls, all of Chinese Han origin, were genotyped by polymerase chain reaction. RESULTS: Both genotype distributions (P=0.033) and allele frequencies (P=0.023, OR = 1.250, 95% CI = 1.031-1.517) of NET-T182C were significantly different between patients and controls, where the T allele was associated with the onset of depression. Both NET-T182C T- and 5- HTTLPR L-carriers had higher baseline HAMD scores (P=0.032 and 0.023, respectively). There was an interaction between NET-T182C and 5-HTTLPR, where the combined genotype distributions were associated with both onset of depression (P=0.006) and the baseline HAMD scores (P=0.007). CONCLUSION: This study suggested a positive relationship between the NET-T182C polymorphism and the susceptibility to depression, and a positive relationship between NET-T182C/5-HTTLPR polymorphisms and the severity of depression.

[Neuron apoptosis induced by 3,4-methylenedioxy methamphetamine and expression of apoptosis-related factors in rat brain].

OBJECTIVE: To study the neuron apoptosis induced by i.p 3,4-methylenedioxy methamphetamine (MDMA) and the expression of apoptosis-related factors in rat brain. METHODS: Twenty rats were divided into 4 groups. In group A, the rats were injected intraperitoneally with single dosage of saline, while the rats of group B, C, D were injected i.p with MDMA in different regimen, which were 20 mg/kg, single injection in group B, 20 mg/ kg twice a day (8 am and 8 pm), for 2 day in group C, as well as 20 mg/kg, twice a day (8 am and 8 pm) for 4 days. Neuron apoptosis were measured by TUNEL, and the expression of Caspase-3 and CytC were detected by immunohistochemistry. RESULTS: Compared with saline group, apoptosis neurons were detected at the related brain regions (such as frontal cortex, hippocampus and striatum) of the rats in MDMA treated groups; Expression of Caspase-3 and CytC was observed at different level. Compared with group B, the number of apoptosis neurons of group C and D increased, and also the apoptosis-related factors in the brain tissue increased (P < 0.05). CONCLUSION: MDMA could induce neurons apoptosis and the expression of apoptosis-related factors such as Caspase-3 and CytC in rat brain.

[A proton magnetic spectroscopy research on hippocampus metabolisms in people with suicide-attempted depressions].

OBJECTIVE: To investigate the metabolic features of hippocampus in people with suicide-attempted depressions. METHODS: Proton resonance spectroscopy (1H-MRS) was performed on the bilateral hippocampi of 24 patients with suicide-attempted depressions and 24 healthy people. The ratios of N-acetylasparte(NAA)/creatine (Cr), choline(Cho)/Cr and myoinositol(mI)/Cr were calculated and used as markers reflecting the metabolic levels of NAA, Cho and mI. The differences between the patients with suicide-attempted depressions and the healthy people were analyzed. The left hippocampus was also compared with the right to determine the asymmetry of the hippocampus metabolic. RESULTS: The ratio of NAA/Cr in the left hippocampus of the patients with suicide-attempted depressions was significantly less than that of the healthy control (P < 0.05). The ratio of NAA/Cr in the left hippocampus was significantly greater than in the right hippocampus of the healthy control (P < 0.05). However, the left-right deference of the ratio of NAA/Cr disappeared in the patients with suicide-attempted depressions. There was an asymmetry between the left and right hippocampi in the healthy people (P < 0.05), but not in the patients with suicide-attempted depressions (P > 0.05). No correlations between the metabolic markers and the course of disease, number of episodes and HAMD17 scores were found in the patients with suicide-attempted depressions (P > 0.05). CONCLUSION: There is a decrease of metabolite in the left hippocampus of patients with suicide-attempted depressions. The asymmetry of the bilateral hippocampi disappears in the patients with suicide-attempted depressions.

[The mental health of children and adolescents from earthquake affected areas].

OBJECTIVE: To examine the mental health status of 11-16 years old children exposed to the 5.12 WenChuan earthquake and to help develop post-traumatic psychological interventions. METHODS: The self-reported Strengths and Difficulties Questionnaire (SDQ) was administered to 540 children (mean age: 13.82+/-0.98, male/female ratio 1.06:1) from the earthquake affected areas one month after the earthquake. RESULTS: (1) The children had an average difficulty score (T score) of 13.41+/-5.71, with 74.6% reporting at least minor perceived difficulties. (2) The children with higher levels of exposure had higher emotional subscale scores (z=-2.323, P=0.020). The female students had higher emotional subscale scores than the male students (z=-4.985, P=0.000). (4) The impact of the difficulties on the children's learning increased with age (z=9.106, P=0.011). (5) The longer the children lived in the transferred safe places, the more likely the children got an abnormal (OR=1.233, P=0.003). CONCLUSION: The mental health status of the children from the earthquake affected areas are influenced by their exposure levels, gender, age and the length of living in transferred safe places. These factors should be taken into considerations when post-traumatic psychological interventions are planned.

Characterization of the complete chloroplast genome of Sanming wild banana (Musa itinerans) and phylogenetic relationships.

Sanming wild banana (Musa itinerans) is a cold resistant wild banana found in Fujian province of China. In this study, we characterized its complete chloroplast genome using BGISEQ-500 sequencing. The chloroplast genome is 171,815 bp in size, containing a pair of IR regions (35,142 bp), a large single copy region (89,995 bp), and a small single copy region (11,464 bp). The whole chloroplast genome contains 111 unique genes, including 78 protein-coding genes, 29 tRNAs, and 4 rRNAs. Phylogenetic maximum likelihood analysis revealed that Sanming wild banana showed the closest relationship with Musa itinerans that collected from Yunnan Province of China.

[Impact of anxiety-depression symptoms on clinical recovery and serum TNF-alpha and IL-6 of patients with burns].

OBJECTIVE: To investigate the impact of anxiety-depression symptoms on the healing of burn wounds and the serum tumor necrosis factor a (TNF-alpha) and interleukin-6 (IL-6) in the patients with moderate and severe burns. METHODS: The patients with moderate and severe burns were divided into anxiety-depression group (24 patients) and non-anxiety-depression group (27 patients)according to the scores of anxiety-depression scores three days after burn measured by Hamilton Rating Scale for Depression (HAMD) and Hamilton Rating Scale for Anxiety (HAMA). The routine therapy was applied for both groups for 30 days. Then the scores of anxiety and depression and the degrees of wound healing were examined. The serum TNF-alpha IL-6 were measured with enzyme linked immunosorbent assay (ELISA). RESULTS: No significant decrease of the scores of depression was found before and after treatments in the anxiety-depression group (P > 0.05). The days that were needed for healing the wounds and the serum TNF-alpha and IL-6 increased in the patients with depression compared with the control group (P < 0.05). CONCLUSION: There are anxious and depressive reactions to burns by the patients. The continuous negative emotion can induce the increase of serum TNF-alpha and IL-6 and lead to delay of the healing of the burn wounds.

5-HTR1A and 5-HTR2A genetic polymorphisms and SSRI antidepressant response in depressive Chinese patients.

OBJECTIVE: Genetic variabilities within the serotoninergic system may predict response or remission to antidepressant drugs. Several serotonin receptor (5-HTR) gene polymorphisms have been associated with susceptibility to psychiatric diseases. In this study, we analyzed the correlation between 5-HTR1A and 5-HTR2A polymorphisms and response or remission to selective serotonin reuptake inhibitors (SSRIs) drugs. METHODS: Two hundred and ninety patients who met the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition criteria for major depressive disorder were involved in this study. SSRIs (fluoxetine, paroxetine, citalopram, or sertraline) were selected randomly for treatment. The Hamilton Rating Scale for Depression was used to evaluate the antidepressant effect. To assess 5-HTR gene variabilities, two single-nucleotide polymorphisms in 5-HTR1A (rs1364043 and rs10042486) and three in 5-HTR2A (rs6311, rs6313, and rs17289304) were genotyped by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using the Sequenom MassARRAY Analyzer 4 system. RESULTS: There were 220 responders and 70 nonresponders (120 remissioners and 170 nonremissioners) after 6 weeks of treatment. We found no association between any of the five 5-HTR1A and 5-HTR2A gene polymorphisms and antidepressant drug response or remission (P>0.05). It is worth mentioning that TT genotype frequency of rs10042486 was significantly different from the CT genotype frequency between responders and nonresponders, although the significance was not maintained after correcting for multiple testing. CONCLUSION: Thus, 5-HTR1A and 5-HTR2A gene polymorphisms may not play an important role in antidepressant drug response or remission.

Substrate-Controlled, One-Pot Synthesis: Access to Chiral Chroman-2-one and Polycyclic Derivatives.

Based on the appropriate choice of electrophiles, one-pot, multicomponent, enantioselective domino reactions have been realized which contain a five-step sequence and provide highly efficient access to potentially bioactive chroman-2-one derivatives as a single diastereoisomer with excellent enantioselectivities and in high yields. This new strategy could significantly improve the previous protocol by directly starting from commercial 2-hydroxybenzaldehydes rather than preformed lactols, which have to be synthesized in several additional steps.

Efficacy of atropine combined with paroxetine in vagus nerve excitatory panic disorder.

Panic disorder is often associated with the autonomic nervous system pattern - sympathetic activation and parasympathetic (vagal) withdrawal. However, we present one special case here to show a totally reversed pathogenesis - vagal activation occupying the leading role, which requires atropine to cure the patient's symptoms. Through this report, it is reasonably proven that panic disorder may be a heterogeneous condition, whose mechanism might be the imbalance between the sympathetic and parasympathetic tone.

[Association of psychosocial factors and platelet 5-HT with peptic ulcer].

OBJECTIVE: To scrutinize the association of psychosocial factors especially emotional factors with peptic ulcer. METHODS: Eighty-nine peptic ulcer patients and 50 normal controls were investigated by means of questionnaires for personality, alexithymia, social support, life events, and their platelet 5-HT levels were determined. RESULTS: By comparison with normal controls, the peptic ulcer patients had: (1) higher scores on the subscales such as Hs, D, Pt, Sc and Hy of personality; (2) Higher scores on HAMA, HAMD and Toronto alexithymia scale (TAS); (3) Significantly lower platelet 5-HT concentration. And in the ulcer patients who were not infected with HP (called HP negative), the area of their ulcer was found to have a positive correlation with HAMA and TAS scores (r=0.74, P<0.01; r=0.45, P<0.05). CONCLUSION: (1) There was a correlation between the onset of peptic ulcer and personality or manner of emotional expression. (2) There may be two subtypes of ulcer patients: one of it may be due to HP infection and the other may be more closely associated with negative emotion and manner of emotional expression; for the latter one, anxiolytics deserve to be included in the routine treatments.