Investigation of chemokine receptor CCR2V64Il gene polymorphism and migraine without aura in the Iranian population.

BACKGROUND AND OBJECTIVES: Migraine is a multifactorial common neurovascular disease with a polygenic inheritance. Inflammation plays an important part in migraine pathophysiology. C-C chemokine receptor 2 (CCR2) is an important chemokine for monocyte aggregation and transendothelial monocyte migration. The aim of our study was to investigate the association of migraine with CCR2V64Il polymorphism in the Iranian population. METHODS: We assessed 103 patients with newly diagnosed migraine and 100 healthy subjects. Genomic DNA samples were extracted from peripheral blood and genotypes of CCR2V64Il gene polymorphism were determined. For measuring the severity of headache, every patient filled out the MIGSEV questionnaire. RESULTS: There were no significant differences in the distribution of both 64Il allele and heterozygote (GA) genotype of CCR2 gene polymorphism (P = 0.396; OR = 0.92, 95% CI = 0.50-1.67 and P = 0.388; OR = 0.91, 95% CI = 0.47-1.73, resp.) between case and control groups. There was no significant difference of alleles frequency between three grades of MIGSEV (P = 0.922). CONCLUSIONS: In conclusion our results revealed no association between CCR2V64Il polymorphism and susceptibility to migraine and also headache severity in the Iranian population.

Association between Ala379Val polymorphism of lipoprotein-associated phospholipase A2 and migraine without aura in Iranian population.

BACKGROUND: Migraine is a common neurovascular disorder with multifactorial and polygenic inheritance. The aim of this study was to investigate the association of a migraine without aura and Ala379Val polymorphism of lipoprotein-associated phospholipase A2 (Lp-PLA2) gene in the Iranian population. METHODS: In this study, 103 migraine patients and 100 healthy controls were enrolled. DNA samples were extracted and the Ala379Val polymorphism of Lp-PLA2 gene was investigated. To assess severity of a headache, patients filled out the headache impact test (HIT-6) and migraine severity (MIGSEV) questionnaires. RESULTS: Allele V had significantly lower frequency in the case group than control subjects [P = 0.001, odds ratio (OR) = 0.25, confidence interval (CI): 0.15-0.40]. The frequency of migraine patients that were a carrier of V allele (V/V and A/V) was statistically significant lower than the control group (P = 0.003, OR = 2.39, CI: 1.35-4.23). There was no significant difference of alleles frequency between three grades of MIGSEV (P = 0.316). Furthermore, total HIT-6 score was not significantly different between different genotypes (P = 0.466). CONCLUSION: Our results showed that Ala379Val gene polymorphism of LP-PLA2 is associated with lower risk of migraine but not with severity of headaches in an Iranian population.

Association of the long pentraxin PTX3 gene polymorphism (rs3816527) with migraine in an Iranian population.

BACKGROUND: Migraine is a common neurovascular disorder with multifactorial and polygenic inheritance. It has been shown that migraine may be a form of sterile neurogenic inflammation. Pentaxins 3 (PTX3) has been detected in brain during inflammatory responses. The aim of our study was to investigate the association of rs3816527 polymorphism of the PTX3 gene and migraine in an Iranian population. METHOD: We included 103 newly diagnosed migraine patients and 148 healthy subjects as control group. Genomic DNA samples extracted from the peripheral blood and genotypes of PTX3 rs3816527 gene polymorphism were determined. The patients filled out HIT-6 questionnaire as a scale to evaluate the severity of headache. RESULTS: The genotype frequency of PTX3 was significantly different between the migraine patients and the control subjects. CC variant homozygote genotype was statistically more frequent in the patients than in the controls (P<0.05; OR=1.74, 95% CI=1.04-2.94). Also the C allele was not significantly more frequent in the patients (P=0.096; OR=1.27, 95% CI=0.88-1.85). A separate analysis in male and female subjects showed no significant differences between the different genotypes and phenotypes of PTX3 rs3816527 gene and susceptibility to migraine in female subjects. Total HIT-6 score was significantly different between three PTX3 genotypes (P=0.008). CONCLUSION: In conclusion our results showed the association between the PTX3 rs3816527 gene polymorphism with susceptibility to migraine only in the male patients. Also total HIT-6 scores as a scale for assessment of the severity were related to the PTX3 rs3816527 gene polymorphism. But this relation was not established by headache frequency.

Association between promoter region of the uPAR (rs344781) gene polymorphism in genetic susceptibility to migraine without aura in three Iranian hospitals.

INTRODUCTION: Migraine is a chronic neurological disorder. Inflammation has a key role in migraine pathophysiology. Urokinase plasminogen activator receptor (uPAR) directly involves in inflammatory conditions by facilitating migration of inflammatory cells to different tissues. The aim of this study was to investigate whether uPAR rs344781, common genetic polymorphism in the uPAR promoter region, might be associated with migraine without aura susceptibility in an Iranian population. METHODS: We enrolled 103 newly diagnosed patients with migraine and 100 healthy controls. Peripheral blood sample was used for DNA extraction and uPAR rs344781 gene polymorphism was determined. Patients filled HIT-6 as a tool to evaluate headache severity. RESULTS: The genotype frequency of uPAR is significantly different between migraine patients and control subjects. Heterozygote genotype (AG) was statistically more frequent in the patients than the controls (P=0.001; OR=2.67, 95% CI=1.51-4.7). Also G allele was more frequent in the patients. Total HIT-6 score was not significantly different between heterozygote and homozygote patients (55.50±2.22 vs. 49.60±3.68 respectively, P=0.075). CONCLUSION: In conclusion, our study showed a significant association between uPAR rs344781 gene promoter polymorphism and migraine without aura susceptibility but not with headache severity.