RESUMEN
The effect of Escherichia coli (E. coli) cells on two phospholipids [dipalmitoyl phosphatidylcholine (DPPC) and dimyristoyl phosphatidylcholine (DMPC)] monolayers at the surface of a 1.5 wt% NaCl salt solution has been investigated using surface tension measurement and Brewster angle microscopy. The results showed that a DPPC monolayer that has an elastic structure was changed in morphology by interaction with E. coli cells, whereas a DMPC monolayer that has an expandable structure did not change in morphology. In particular, the morphology changed significantly around the liquid-expanded (LE)-liquid-condensed (LC) phase transition point for the DPPC monolayer. It was found that the LE-LC phase transition range in a DPPC monolayer was sensitive to influence from the outside of the monolayer such as the action of E. coli cells. Such a monolayer has the potential for application as a membrane sensor for detecting a small amount of bacteria in a short time.