RESUMEN
Traditionally, there is a trade-off between the numerical aperture and field of view for a microscope objective. Diffractive lens arrays (DLAs) with overlapping apertures are used to overcome such a problem. A spot array with an NA up to 0.83 and a pitch of 75 µm is produced by the proposed DLA at a wavelength of 488 nm. By measurement of the fluorescence beads, the DLA-based confocal setup shows the capability of high-resolution measurement over an area of 3mm×3mm with a 2.5×0.07NA objective. Further, the proposed fluorescence microscope is insensitive to optical aberrations, which has been demonstrated by imaging with a simple doublet lens.
RESUMEN
High resolution and large fields of view are difficult to achieve simultaneously by microscope objectives. In this work, we develop a reflection confocal microscope based on diffractive lens arrays to solve the problem. We demonstrate a prototype that generates a spot array with a numerical aperture of 0.78. Laterally, experiments show a spatial cutoff frequency of 1024 lp/mm by a 0.15 NA objective, and 912 lp/mm by a 0.07 NA objective with a 785 nm diode laser. Axially, an average height of 961 nm with a standard deviation of 49 nm is measured with a 925.5 nm calibrated step height target.
RESUMEN
Illumination systems with tunable spectrums have been receiving an increasing amount of attention due to their wide application and unique capability. This paper proposes a programmable light source in the visible range based on the combination of a prism and an echelle grating. A supercontinuum laser is utilized as the primary source, whose echellogram is projected to a digital mirror device for wavelength selection. A complete calibration procedure is developed to generate any target spectrum of choice. Experiments have shown that spectral peaks with a full width at half-maximum of 1 nm can be easily generated and the wavelength tuning resolution can reach as small as 0.01 nm.