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We studied factors affecting osteoporotic hip fracture mortality in Hawai'i, a region with unique geography and racial composition. Men, older adults, higher ASA score, lower BMI, and NHPI race were associated with higher mortality. This is the first study demonstrating increased mortality risk after hip fracture in NHPI patients. PURPOSE: To estimate mortality rates and identify specific risk factors associated with 1-year mortality after osteoporotic hip fracture in Hawai'i. METHODS: A retrospective review of adults (≥ 50 years) hospitalized with an osteoporotic hip fracture at a large multicenter healthcare system in Hawai'i from 2011 to 2019. The Kaplan-Meier curves and log-rank tests examined survival probability by sex, age group, race/ethnicity, primary insurance, body mass index (BMI), and American Society of Anesthesiologists (ASA) physical status classification. After accounting for potential confounders, adjusted hazard ratios (aHR) and 95% confidence intervals (CI) were obtained from Cox proportional hazards regression models. RESULTS: We identified 1755 cases of osteoporotic hip fracture. The cumulative mortality rate 1 year after fracture was 14.4%. Older age (aHR 3.50; 95% CI 2.13-5.76 for ≥ 90 vs 50-69), higher ASA score (aHR 5.21; 95% CI 3.09-8.77 for ASA 4-5 vs 1-2), and Native Hawaiian/Pacific Islander (NHPI) race (aHR 1.84; 95% CI 1.10-3.07 vs. White) were independently associated with higher mortality risk. Female sex (aHR 0.64; 95% CI 0.49-0.84 vs male sex) and higher BMI (aHR 0.35; 95% CI 0.18-0.68 for obese vs underweight) were associated with lower mortality risk. CONCLUSION: In our study, men, older adults, higher ASA score, lower BMI, and NHPI race were associated with significantly higher mortality risk after osteoporotic hip fracture. NHPIs are an especially vulnerable group and comprise a significant portion of Hawai'i's population. Further research is needed to address the causes of higher mortality and interventions to reduce hip fractures and associated mortality.
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Sacrificio de Animales , Artiodáctilos , Especies Introducidas , Investigadores , Colombia , Biodiversidad , Ecología/métodosAsunto(s)
COVID-19/epidemiología , COVID-19/virología , SARS-CoV-2/patogenicidad , Adolescente , COVID-19/diagnóstico , COVID-19/transmisión , Vacunas contra la COVID-19/administración & dosificación , Niño , Humanos , Programas de Inmunización , Opinión Pública , Factores de Tiempo , Uruguay/epidemiologíaAsunto(s)
Investigación Biomédica , COVID-19/epidemiología , Recolección de Datos , Gobierno Federal , Personal de Salud , Política , SARS-CoV-2 , Investigación Biomédica/normas , Brasil/epidemiología , COVID-19/diagnóstico , COVID-19/prevención & control , COVID-19/transmisión , Prueba de COVID-19 , Colombia/epidemiología , Recolección de Datos/normas , Necesidades y Demandas de Servicios de Salud , Humanos , Enfermeras y Enfermeros , Organizaciones , Perú/epidemiología , Médicos , Pobreza/estadística & datos numéricos , Reproducibilidad de los Resultados , Revelación de la Verdad , Venezuela/epidemiologíaAsunto(s)
Vacunas contra la COVID-19/inmunología , COVID-19/epidemiología , COVID-19/prevención & control , Control de Enfermedades Transmisibles/estadística & datos numéricos , Investigadores , Anticuerpos Neutralizantes/análisis , Anticuerpos Neutralizantes/inmunología , COVID-19/inmunología , COVID-19/transmisión , Vacunas contra la COVID-19/administración & dosificación , Control de Enfermedades Transmisibles/legislación & jurisprudencia , Conductas Relacionadas con la Salud , Humanos , Inmunización Secundaria , Máscaras , Distanciamiento Físico , Reino Unido/epidemiologíaAsunto(s)
Vacunas contra la COVID-19/provisión & distribución , Ensayos Clínicos Fase III como Asunto/ética , Salud de la Familia/ética , Gobierno Federal , Política , Opinión Pública , Investigadores/ética , Universidades , Protocolos Clínicos , Ensayos Clínicos Fase III como Asunto/métodos , Femenino , Humanos , Masculino , Perú , ConfianzaRESUMEN
Cannabis is one of the most commonly used recreational drugs worldwide. Rrecent epidemiology studies have linked increased cardiac complications to cannabis use. However, this literature is predominantly based on case incidents and post-mortem investigations. This study elucidates the molecular mechanism of Δ9-tetrahydrocannabinol (THC), and its primary metabolites 11-Hydroxy-Δ9-THC (THC-OH) and 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH). Treatment of cardiac myocytes with THC-OH and THC-COOH increased cell migration and proliferation (p < 0.05), with no effect on cell adhesion, with higher doses (250-100 ng/mL) resulting in increased cell death and significant deterioration in cellular architecture. Conversely, no changes in cell morphology or viability were observed in response to THC. Expression of key ECM proteins α-SMA and collagen were up-regulated in response to THC-OH and THC-COOH treatments with concomitant modulation of PI3K and MAPK signalling. Investigations in the planarian animal model Polycelis nigra demonstrated that treatments with cannabinoid metabolites resulted in increased protein deposition at transection sites while higher doses resulted in significant lethality and decline in regeneration. These results highlight that the key metabolites of cannabis elicit toxic effects independent of the parent and psychoactive compound, with implications for cardiotoxicity relating to hypertrophy and fibrogenesis.
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Cannabis , Alucinógenos , Analgésicos/metabolismo , Animales , Agonistas de Receptores de Cannabinoides , Cannabis/metabolismo , Cannabis/toxicidad , Cardiotoxicidad , Dronabinol/toxicidad , Alucinógenos/metabolismo , Miocitos Cardíacos/metabolismoRESUMEN
ABSTRACT: Taylor, L and Jakeman, JR. The impact of a repeated sprint training program on performance measures in male field hockey players. J Strength Cond Res 36(7): 1984-1988, 2022-Sprint interval training (SIT) has been shown to be effective at improving athletic performance in laboratory studies, but the efficacy of SIT programs incorporated into normal training schedules is poorly considered. This study aimed to investigate the impact of a running SIT intervention applied to competitive athletes within a training program and to consider whether an uphill or flat (horizontal) SIT protocol had different effects on performance changes over time. Eighteen male hockey players (mean ± SD: age, 20.7 ± 0.9 years; hockey training experience, 9.9 ± 3.0 years) completed 2 sessions of SIT per week for 8 weeks, with intensity progressively increasing from 6 sprints in week 1 to 12 sprints in week 8. Subjects were randomly allocated to a flat or uphill (6% gradient) training condition (n = 9) and completed 30-m maximal sprint efforts with a 30-second recovery. Performance measures, including squat jump, 30-m sprint speed, and repeated sprint time, all improved significantly (p ≤ 0.05). Squat jump performance improved by 3.84 (d = 0.8) and 3.55 (d = 0.7) in the flat and uphill groups, respectively. Thirty-meter sprint speed improved by 0.06 (d = -0.4) and 0.10 (d = -0.7), and repeated sprint performance also improved, with the fastest of recorded sprints after intervention being 0.06 and 0.04 faster in the flat and uphill groups, respectively. Supplementing a normal hockey training week with SIT can have a positive impact on performance measures in male university hockey players. Furthermore, using an uphill training modality had a small, nonsignificant additional positive effect to some performance adaptations.
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Rendimiento Atlético , Entrenamiento de Intervalos de Alta Intensidad , Hockey , Carrera , Adulto , Humanos , Masculino , Modalidades de Fisioterapia , Adulto JovenRESUMEN
Ethanol is the most commonly used recreational drug worldwide. This study describes the development and validation of a headspace gas chromatography flame ionisation detection (HS-GC-FID) method using dual columns and detectors for simultaneous separation and quantitation. The use of a dual-column, dual-detector HS-GC-FID to quantitate ethanol is a common analytical technique in forensic toxicology; however, most analytical systems utilise pressure-balance injection rather than a simplified gas-tight syringe, as per this technique. This study is the first to develop and validate a technique that meets the specifications of the United Kingdom's requirements for road traffic toxicology testing using a Shimadzu GC-2014 gas-tight syringe. The calibration ranged from 10 to 400 mg/100 mL, with a target minimum linearity of r2 > 0.999, using tertiary butanol as the internal standard marker. The method has an expanded uncertainty at 99.73% confidence of 3.64% at 80 mg/100 mL, which is the blood alcohol limit for drink driving in England and Wales. In addition, at 200 mg%the limit at which a custodial sentence may be imposed on the defendantthe expanded uncertainty was 1.95%. For both the 80 mg% and 200 mg% concentrations, no bias was present in the analytical method. This method displays sufficient separation for other alcohols, such as methanol, isopropanol, acetaldehyde, and acetone. The validation of this technique complies with the recommended laboratory guidelines set out by United Kingdom and Ireland Association of Forensic Toxicologists (UKIAFT), the recently issued Laboratory 51 guidelines by the United Kingdom Accreditation Service (UKAS), and the criteria set out by the California Code of Regulations (CCR), 17 CCR § 1220.1.
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Etanol , Jeringas , Acetaldehído/análisis , Cromatografía de Gases , Etanol/análisis , Ionización de Llama/métodosRESUMEN
Paediatric atrial fibrillation (AF) is an infrequent entity in the absence of congenital heart disease as children are unlikely to have the structural and functional changes in their myocardium to sustain the arrhythmia. Any child presenting with this arrhythmia needs to be carefully evaluated for concealed cardiac pathology such as cardiomyopathy or inherited arrhythmia syndromes. AF leading to a haemodynamically unstable patient is rare and should prompt synchronised cardioversion, while stable patients can be discussed with a paediatric cardiologist. Tachycardia-induced cardiomyopathy and thromboembolism are possible complications of sustained AF and anticoagulation is usually indicated to prevent the latter. Risk of AF increases with age and body mass index. Obesity and athletics are known risk factors and recurrence can be seen even in the absence of any identifiable underlying pathology.
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Fibrilación Atrial , Fibrilación Atrial/etiología , Fibrilación Atrial/terapia , Niño , Cardioversión Eléctrica , Corazón , Humanos , Pediatras , RecurrenciaRESUMEN
Cocaine is one of the most widely abused illicit drugs worldwide and has long been recognised as an agent of cardiac dysfunction in numerous cases of drug overdose. Cocaine has previously been shown to up-regulate cytoskeletal rearrangements and morphological changes in numerous tissues; however, previous literature observes such changes primarily in clinical case reports and addiction studies. An investigation into the fundamental cytoskeletal parameters of migration, adhesion and proliferation were studied to determine the cytoskeletal and cytotoxic basis of cocaine in cardiac cells. Treatment of cardiac myocytes with cocaine increased cell migration and adhesion (p < 0.05), with no effect on cell proliferation, except with higher doses eliciting (1-10 µg/mL) its diminution and increase in cell death. Cocaine downregulated phosphorylation of cofilin, decreased expression of adhesion modulators (integrin-ß3) and increased expression of ezirin within three hours of 1 µg/mL treatments. These functional responses were associated with changes in cellular morphology, including alterations in membrane stability and a stellate-like phenotype with less compaction between cells. Higher dose treatments of cocaine (5-10 µg/mL) were associated with significant cardiomyocyte cell death (p < 0.05) and loss of cellular architecture. These results highlight the importance of cocaine in mediating cardiomyocyte function and cytotoxicity associated with the possible loss of intercellular contacts required to maintain normal cell viability, with implications for cardiotoxicity relating to hypertrophy and fibrogenesis.
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Cardiotoxicidad/patología , Cocaína/toxicidad , Citoesqueleto/patología , Miocitos Cardíacos/patología , Vasoconstrictores/toxicidad , Animales , Cardiotoxicidad/etiología , Adhesión Celular , Movimiento Celular , Supervivencia Celular , Células Cultivadas , Citoesqueleto/efectos de los fármacos , Miocitos Cardíacos/efectos de los fármacos , RatasRESUMEN
BACKGROUND: Concentrated cherry juice reportedly contains melatonin which, in turn, has been highlighted as an important regulator in initiating sleep. AIM: The present investigation aims to clarify whether Night Time Recharge (NTR), a marketed sleep aid containing cherry extract, improves key sleep parameters in young, active adults with mildly poor sleep. METHODS: A double-blind, randomized, placebo-controlled, cross-over study design was employed. Twenty participants (nine female) consumed either NTR or a placebo for seven days. Accelerometers were used to assess sleep quality and physical activity levels. Urinary levels of 6-sulphatoxymelatonin (6-SMT), a marker of melatonin synthesis, was assessed via enzyme-linked immunosorbent assay. RESULTS: 6-SMT levels increased following NTR treatment (28.95 ng/ml) compared with placebo (4.0 ng/ml) (p < 0.001). There was also a significant difference (p = 0.047) in dietary tryptophan consumption during the NTR treatment (1236 mg) versus placebo (1149 mg). No trace of melatonin was detected from our analysis of the supplement. NTR had no significant effect on any sleep parameters with the exception of sleep latency (p = 0.001). CONCLUSIONS: As chemical analysis of NTR by liquid-chromatography mass-spectrometry identified no detectable melatonin, the tryptophan content of the supplement is a likely reason for improvement in sleep latency. These results are in contrast to previous studies which have found a positive effect on sleep following cherry supplementation. Future work should focus on sleep latency and investigating whether cherry juice is effective in participants with problems in initiating sleep.
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Suplementos Dietéticos/análisis , Melatonina/análogos & derivados , Melatonina/orina , Prunus avium/química , Latencia del Sueño/efectos de los fármacos , Triptófano/orina , Acelerometría , Estudios Cruzados , Método Doble Ciego , Inglaterra , Femenino , Cromatografía de Gases y Espectrometría de Masas , Humanos , Masculino , Adulto JovenRESUMEN
STUDY OBJECTIVE: Point-of-care ultrasonography protocols are commonly used in the initial management of patients with undifferentiated hypotension in the emergency department (ED). There is little published evidence for any mortality benefit. We compare the effect of a point-of-care ultrasonography protocol versus standard care without point-of-care ultrasonography for survival and clinical outcomes. METHODS: This international, multicenter, randomized controlled trial recruited from 6 centers in North America and South Africa and included selected hypotensive patients (systolic blood pressure <100 mm Hg or shock index >1) randomized to early point-of-care ultrasonography plus standard care versus standard care without point-of-care ultrasonography. Diagnoses were recorded at 0 and 60 minutes. The primary outcome measure was survival to 30 days or hospital discharge. Secondary outcome measures included initial treatment and investigations, admissions, and length of stay. RESULTS: Follow-up was completed for 270 of 273 patients. The most common diagnosis in more than half the patients was occult sepsis. We found no important differences between groups for the primary outcome of survival (point-of-care ultrasonography group 104 of 136 patients versus standard care 102 of 134 patients; difference 0.35%; 95% binomial confidence interval [CI] -10.2% to 11.0%), survival in North America (point-of-care ultrasonography group 76 of 89 patients versus standard care 72 of 88 patients; difference 3.6%; CI -8.1% to 15.3%), and survival in South Africa (point-of-care ultrasonography group 28 of 47 patients versus standard care 30 of 46 patients; difference 5.6%; CI -15.2% to 26.0%). There were no important differences in rates of computed tomography (CT) scanning, inotrope or intravenous fluid use, and ICU or total length of stay. CONCLUSION: To our knowledge, this is the first randomized controlled trial to compare point-of-care ultrasonography to standard care without point-of-care ultrasonography in undifferentiated hypotensive ED patients. We did not find any benefits for survival, length of stay, rates of CT scanning, inotrope use, or fluid administration. The addition of a point-of-care ultrasonography protocol to standard care may not translate into a survival benefit in this group.
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Protocolos Clínicos , Hipotensión/diagnóstico , Sistemas de Atención de Punto/estadística & datos numéricos , Ultrasonografía/estadística & datos numéricos , Servicio de Urgencia en Hospital , Femenino , Humanos , Hipotensión/diagnóstico por imagen , Hipotensión/mortalidad , Masculino , Persona de Mediana Edad , América del Norte , Mejoramiento de la Calidad , SudáfricaRESUMEN
α-Tocopherol (α-TOH) is the primary lipophilic radical trapping antioxidant in human tissues. Oxidative catabolism of α-tocopherol (αTOH) is initiated by ω-hydroxylation of the terminal carbon (C-13) of the isoprenoid sidechain followed by oxidative transformations that sequentially truncate the chain to yield the 2,5,7,8-tetramethyl(3'carboxyethyl)-6-hydroxychroman (α-CEHC). After conjugation to glucuronic acid, 3'-carboxyethyl-6-hydroxychroman glucuronide is excreted in urine. We report here that the same enzyme that accomplishes this task, the cytochrome P450 monooxygenase CYP-4F2, can also ω-hydroxylate the terminal carbon of α-tocopheryl quinone. A standard sample of ω-OH-α-tocopheryl quinone (ω-OH-α-TQ) was synthesized as a mixture of stereoisomers by allylic oxidation of α-tocotrienol using SeO2 followed by double-bond reduction and oxidation to the quinone. After incubating human liver microsomes or insect cell microsomes expressing only recombinant human CYP-4F2, cytochrome b5, and NADPH P450 reductase with d6-α-tocopheryl quinone (d6-αTQ), we showed that the ω-hydroxylated (13-OH) d6-α-TQ was produced. We further identified the production of the terminal carboxylic acid d6-13-COOH-αTQ. The ramifications of this discovery to the understanding of tocopherol utilization and metabolism, including the quantitative importance of the αTQ-ω-hydroxylase pathway in humans, are discussed.
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Familia 4 del Citocromo P450/metabolismo , Microsomas Hepáticos/metabolismo , Vitamina E/análogos & derivados , Vitamina E/metabolismo , Animales , Humanos , Hidroxilación , Insectos , Oxidación-Reducción , Proteínas Recombinantes/metabolismoRESUMEN
The aquaporin family of integral membrane proteins is composed of channels that mediate cellular water flow. Aquaporin 4 (AQP4) is highly expressed in the glial cells of the central nervous system and facilitates the osmotically driven pathological brain swelling associated with stroke and traumatic brain injury. Here we show that AQP4 cell surface expression can be rapidly and reversibly regulated in response to changes of tonicity in primary cortical rat astrocytes and in transfected HEK293 cells. The translocation mechanism involves PKA activation, influx of extracellular calcium, and activation of calmodulin. We identify five putative PKA phosphorylation sites and use site-directed mutagenesis to show that only phosphorylation at one of these sites, serine 276, is necessary for the translocation response. We discuss our findings in the context of the identification of new therapeutic approaches to treating brain edema.
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Acuaporina 4/metabolismo , Edema Encefálico/metabolismo , Secuencias de Aminoácidos , Animales , Acuaporina 4/química , Acuaporina 4/genética , Astrocitos/metabolismo , Edema Encefálico/genética , Calcio/metabolismo , Calmodulina/metabolismo , Células Cultivadas , Proteínas Quinasas Dependientes de AMP Cíclico/metabolismo , Células HEK293 , Humanos , Presión Osmótica , Fosforilación , Transporte de Proteínas , RatasRESUMEN
Objectives: While our understanding of coronavirus disease 2019 (COVID-19) has evolved, uncertainty remains regarding utility of previously established pulmonary embolism (PE) screening guidelines in patients with COVID-19. Many studies have investigated the efficacy of D-dimer (DD) screenings for patients with COVID-19 admitted to inpatient services, but few have evaluated patients in the emergency department (ED). The purpose of this study was to investigate utility of DD threshold for PE screening in patients with COVID-19 presenting to the ED. Methods: This was a retrospective, multicenter cohort including patients presenting to three EDs between March 1, 2020 and February 1, 2021 who tested positive for COVID-19 during ED visit or in 60 days prior to presentation and had DD ordered in ED. Patients were grouped by those who underwent computed tomography pulmonary angiogram (CTPA) to evaluate for PE and those who did not, and descriptive statistics were performed. Those who underwent CTPA were further divided into PE-positive and PE-negative groups. The discriminative ability of DD in predicting PE in patients with COVID-19 was analyzed using the receiver operating characteristic (ROC) curve. Results: A total of 570 patients with COVID-19 were included in the study, of which 107 underwent CTPA to evaluate for PE. History of diabetes, elevated glucose, elevated lactate dehydrogenase, elevated white blood cell count, elevated platelets, elevated respiratory rate, and lower temperature were associated with increased risk for PE. Compared to those without PE, patients with PE were significantly more likely to be hospitalized (100% vs. 82%, p = 0.020) and admitted to the ICU (64% vs. 24%, p = 0.002). Those with PE had a significantly higher median DD value (21,177 ng/mL) compared to PE-negative group (952 ng/mL, p < 0.001). The ROC curve for DD in predicting PE had an area under the curve of 0.91 (95% confidence interval [0.84, 0.98]). In our study population, the optimal DD threshold for predicting PE was 1815 ng/mL (sensitivity 93% and specificity 80%). A conservative threshold of 1089 ng/mL could be used with sensitivity 100% and specificity 58%. Conclusion: DD is often elevated in patients with COVID-19, regardless of PE. While the classically used DD cutoff is 500 ng/mL, our study demonstrated a threshold of 1089 ng/mL safely predicted PE in patients with COVID-19 .