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The selection of different organic ligands when synthesizing metal organic framework (MOFs) can change their effects on the adsorption performance. Here, four La-MOFs adsorbents (La-SA, La-FA, La-TA and La-OA) with different organic ligands and structures were synthesized by solvothermal method for phosphate adsorption, and the relationship between their adsorption properties and structures was established. Among four La-MOFs, their phosphate adsorption capacities and adsorption rates followed La-SA > La-FA > La-TA > La-OA. The results indicated that average pore diameter played a key role in phosphate adsorption and there was a positive correlation between average pore diameter and adsorption capacity (R2 = 0.86). Coexisting ion experiments showed that phosphate adsorptions on three La-MOFs (La-SA, La-FA and La-TA) were inhibited in the presence of CO32- and HCO3-. The inhibition of CO32- was the most pronounced and the results of redundancy analysis pointed out that it was mainly due to the change of pH value. In contrast, La-OA showed enhanced phosphate adsorption in the presence of CO32- and HCO3-, and the combination of pH experiments showed that phosphate adsorption by La-OA was increased under alkaline conditions. Further combined with FT-IR, XRD, high resolution energy spectra of XPS (La 3d, P 2p and O 1s) and XANES, the adsorption mechanisms were derived electrostatic attraction, chemical precipitation and inner sphere complexation, and the last two were identified as the main mechanisms. Moreover, it can be identified from XPS 2p that the phosphate adsorption on La-FA and La-OA were mainly in the LaPO4 state, while La-SA and La-TA mainly existed in the form of LaPO4·xH2O crystals and inner sphere complexes. From the perspective of material morphology, this work provides a thought for the rational design of MOFs with adjustable properties for phosphate adsorption.
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Estructuras Metalorgánicas , Contaminantes Químicos del Agua , Fosfatos/química , Adsorción , Espectroscopía Infrarroja por Transformada de Fourier , Ligandos , Lantano/química , CinéticaRESUMEN
Discharge of saline organic wastewater is increasing worldwide, yet how salt stress disrupts the microbial community's structure and metabolism in bioreactors has not been systematically investigated. The non-adapted anaerobic granular sludge was inoculated into wastewater with varying salt concentration (ranging from 0% to 5%) to examine the effects of salt stress on the structure and function of the anaerobic microbial community. Result indicated that salt stress had a significant impact on the metabolic function and community structure of the anaerobic granular sludge. Specifically, we observed a notable reduction in methane production in response to all salt stress treatments (r = -0.97, p < 0.01), while an unexpected increase in butyrate production (r = 0.91, p < 0.01) under moderate salt stress (1-3%) with ethanol and acetate as carbon sources. In addition, analysis of microbiome structures and networks demonstrated that as the degree of salt stress increased, the networks exhibited lower connectance and increased compartmentalization. The abundance of interaction partners (methanogenic archaea and syntrophic bacteria) decreased under salt stress. In contrast, the abundance of chain elongation bacteria, specifically Clostridium kluyveri, increased under moderate salt stress (1-3%). As a consequence, the microbial carbon metabolism patterns shifted from cooperative mode (methanogenesis) to independent mode (carbon chain elongation) under moderate salt stress. This study provides evidence that salt stress altered the anaerobic microbial community and carbon metabolism characteristics, and suggests potential guidance for steering the microbiota to promote resource conversion in saline organic wastewater treatment.
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Microbiota , Aguas Residuales , Aguas del Alcantarillado/química , Anaerobiosis , Carbono/metabolismo , Bacterias/metabolismo , Reactores Biológicos/microbiología , MetanoRESUMEN
Bioremediation is one of the most promising strategies for heavy metal immobilization. A new remediation system was demonstrated in this research, which combined phosphate solubilizing bacteria (PSB) with nZVI@Carbon/Phosphate (nZVI@C/P) composite to remediate lead contaminated soil. Experimental results indicated that the new system (nZVI@C/P + PSB) could effectively convert the labile Pb into the stable fraction after 30 days of incubation, which increased the maximum residual fraction percentage of Pb by 70.58%. The characterization results showed that lead may exist in the forms of Pb5(PO4)3Cl, PbSO4 and 3PbCO3·2Pb(OH)2·H2O in the soil treated with nZVI@C/P + PSB. Meanwhile, soil enzyme activities and Leclercia abundance were enhanced in the treated soil compared with CK during the incubation time. In addition, the specialized functions (e.g. ABC transporters, siderophore metabolism, sulfur metabolism and phosphorus metabolism) in PSB and nZVI@C/P + PSB group were also enhanced. These phenomena proved that the key soil metabolic functions may be maintained and enhanced through the synergistic effect of incubated PSB and nZVI@C/P. The study demonstrated that this new bioremediation system provided feasible way to improve the efficacy for lead contaminated soil remediation.
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Restauración y Remediación Ambiental , Contaminantes del Suelo , Fosfatos/química , Biodegradación Ambiental , Carbono/metabolismo , Plomo , Suelo/química , Contaminantes del Suelo/química , Enterobacteriaceae , Bacterias/metabolismoRESUMEN
Legacy phosphorus (P) in soil, accumulated over several years of fertilizer application in excess of crop demand, represents a huge and largely untapped resource. P activators can increase the availability of this P to plants by accelerating its transformation into soluble P fractions. In this study, we evaluated the potentials of four "P activators" (oxalic acid, lignin, phytase and ascorbic acid) to increase plant available P in a laboratory incubation experiment with two P-deficient calcareous soils used for wheat production. Samples were analysed for Olsen P, phosphomonoesterase and with Hedley sequential P fractionation. All four treatments had significant effects on different soil P fractions. Oxalic acid mainly enhanced inorganic P (Pi) solubility from the HCl-extractable P pool. Lignin enhanced P lability from the NaOH-, HCl- and residual-P pools. Phytase and ascorbic acid principally affected the organic P fractions (Po). Oxalic acid and lignin showed most potential to improve P (H2O-P, NaHCO3-Pi and NaHCO3-Po) availability, which increased by 110-419% and 4.1-122%, respectively. These findings illustrated the potential mechanisms responsible for P release associated with different P activators and reinforced the case for their use in increasing legacy P availability for agriculture in calcareous soils.
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Fósforo , Suelo , Agricultura , Fertilizantes , TriticumRESUMEN
Phosphate solubilizing bacteria (PSB) were isolated from heavy metal contaminated soils and their potentials for lead (Pb) immobilization in soil were studied in this paper. 53 PSB strains were isolated and their minimum inhibitory concentrations (MICs) for lead were determined. The results showed that strains B3, B4, B10, F2-1 and L1-5 had relatively high lead resistant capabilities with MICs ranged from 0.5 to 8â¯mM, and were identified as species of Leclercia adecarboxylata and Pseudomonas putida by 16S rRNA. The most efficient PSB strains could solubilize 200â¯mgâ¯L-1 of P, and phosphate solubilizing capacity of those PSBs was related to the concentrations of organic acids, acid phosphatase activity and pH. Furthermore, the growth pattern of L1-5 strain with the presence of Pb(NO3)2 was observed and a mechanism of biomineralization of lead ions in bacteria biomass was determined by FT-IR and XRD analyses. Results showed that high concentration of lead can inhibit the growth of PSBs, and L1-5 isolate can transform lead ions into lead hydroxyapatite and pyromorphite. The PSBs, which possessing the properties of immobilizing lead through phosphate solubilization and biomineralization could be exploited for bioremediation of Pb polluted soils in future.
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Metales Pesados , Contaminantes del Suelo , Plomo , Fosfatos , ARN Ribosómico 16S , Suelo , Microbiología del Suelo , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
The bioaccumulation and toxicity of heavy metals are serious threats to human activities and ecological health. The exploitation of environmentally friendly passivated materials is major importance for the remediation of heavy metal contaminated soil. This research developed a new type of environmental functional material with a core-shell structure, which is an iron-based material functionalized with phosphorus and carbon from sludge for heavy metal pollution remediation. The results indicated that the C/P@Fe exhibits excellent heavy metal removal ability, and the maximum removal rates of the two heavy metals in simulated wastewater could reach 100% under optimum reaction conditions. It also effectively converts the labile Cr/Pb into the stable fraction after 28 days of incubation, which increased the maximum residual fraction percentage of Cr and Pb by 32.43% and 160% in soil. Further analysis found that the carbon layer wrapped around the iron base could improve the electron transport efficiency of reducing iron, phosphorus and ferrum could react with heavy metal ions to form stable minerals, such as FeCr2O4, FeO·Cr2O3, Pb5(PO4)3OH, PbCO3, 2PbCO3·Pb(OH)2 and PbS, after reacting with C/P@Fe. The study demonstrated that the Iron-based materials functionalized with carbon and phosphorus from sludge provided a more efficient way to remove heavy metals.
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Carbono , Cromo , Hierro , Plomo , Fósforo , Aguas del Alcantarillado , Contaminantes del Suelo , Aguas Residuales , Fósforo/química , Aguas del Alcantarillado/química , Hierro/química , Carbono/química , Aguas Residuales/química , Plomo/química , Contaminantes del Suelo/química , Contaminantes del Suelo/análisis , Cromo/química , Suelo/química , Minerales/química , Metales Pesados/química , Restauración y Remediación Ambiental/métodos , Contaminantes Químicos del Agua/químicaRESUMEN
The effects of humic acids (HA) and fulvic acids (FA) on the fate of Cd in anaerobic environment upon microbial reduction of Cd-bearing ferrihydrite (Fh) with Geobacter metallireducens were investigated. The results showed that HA and FA could promote the reductive dissolution of Fh and the formation of vivianite. After incubation of 38 d, vivianite accounted for 47.19%, 59.22%, and 48.53% of total Fe in biological control batch (BCK), HA and FA batches (C/Fe molar ratio of 1.0), respectively, by Mössbauer spectroscopy analysis. In terms of Cd, HA and FA could promote the release of adsorbed Cd during the initial bioreduction process, but reassuringly, after 38 d the dissolved Cd with HA and FA addition batches were 0.58-0.91 and 0.99-1.08 times of the BCK, respectively. The proportions of residual Cd in HA batches were higher than FA and BCK batches, indicating that HA was better than FA in immobilizing Cd. This might be because the quinone groups in HA could act as electron shuttle. This study showed that HA facilitated the transformation of vivianite better than FA, and Cd can be stabilized by resorption or co-precipitation with vivianite, providing a theoretical support for the translocation of Cd in sediment-water interface.
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Cadmio , Sustancias Húmicas , Cadmio/química , Compuestos Férricos/química , Hierro/químicaRESUMEN
Secondary mineralization is a promising method for remediating cadmium (Cd) pollution in sediments, but the poor stability of Cd-containing secondary minerals is a bottleneck that limits the development of this approach. The existence of phosphate can enhance the formation of stable secondary minerals and points a new direction for Cd immobilization. In this research, a novel syntrophic system composed of phosphate solubilizing bacteria (PSB) and dissimilatory iron reducing bacteria (DIRB) was established and the effect and mechanism of Cd immobilization in the system were also explored. The results showed that under the conditions of DIRB:PSB (V:V)= 3:1, syntrophic bacteria dosage of 5% and glucose dosage of 5 g/L, Cd incorporated in the secondary minerals could account for about 60% of the total Cd. In the pH range of 5-9, alkaline environment was conducive to the immobilization of Cd and the percentage of combined Cd was up to 58%, while the combined Cd in secondary minerals decreased from 62% to 56% with the increase of initial Cd concentration from 0.1 to 0.3 mmol/L. In addition, XRD, XPS, Mössbauer and other characterization results showed that secondary minerals, such as Cd exchange hydroxyapatite (Cd-HAP) and kryzhanovskite (Fe3(PO4)2(OH)3) were formed in this new system. The established syntrophic system of PSB and DIRB is thus a prospective bioremediation technology for Cd immobilization in sediments and can avoid the potential risk might be caused by the addition of phosphorus-containing materials.
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Fosfatos , Contaminantes del Suelo , Fosfatos/química , Cadmio/química , Anaerobiosis , Estudios Prospectivos , Minerales/química , Bacterias , Biodegradación Ambiental , Hierro/química , Suelo/química , Contaminantes del Suelo/análisisRESUMEN
The components of waste cooking oil (WCO) are complex and contain toxic substances, which are difficult to treat biologically. Pseudomonas aeruginosa WO2 was isolated from oily sludge by an anaerobic enrichment-aerobic screening method, which could efficiently utilize WCO and produce rhamnolipid. The effects of nutrients and culture conditions on bacterial growth and lipase activity were investigated to optimize the fermentation of WCO. The results showed that strain WO2 utilized 92.25% of WCO and produced 3.03 g/L of rhamnolipid at 120 h. Compared with inorganic sources, the organic nitrogen source stabilized the pH of fermentation medium, improved lipase activity (up to 19.98 U/mL), and promoted the utilization of WCO. Furthermore, the WO2 strain exhibited inferior utilization ability of the soluble starch contained in food waste, but superior salt stress up to 60 g/L. These unique characteristics demonstrate the potential of Pseudomonas aeruginosa WO2 for the utilization of high-salinity oily organic waste or wastewater.
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Pseudomonas aeruginosa , Eliminación de Residuos , Culinaria , Alimentos , Glucolípidos , Tensoactivos/químicaRESUMEN
Controlling eutrophication and recovering phosphate from water bodies are hot issues in the 21st century. Adsorption is considered to be the best method for phosphate removal because of its high adsorption efficiency and fast removal rate. Among the many adsorbents, lanthanum (La)-based adsorbents have been paid more and more attention due to their strong affinity to phosphorus. This paper reviews research of phosphate adsorption on La-based adsorbents in different La forms, including lanthanum oxide/hydroxide, lanthanum mixed metal oxide/hydroxide, lanthanum carbonate, La3+, La-based metal-organic framework (La-MOF) and La-MOF derivatives. The La-based adsorbents can be loaded on many carriers, such as carbon material, clay minerals, porous silica, polymers, industrial wastes, and others. We find that lanthanum oxide/hydroxide and La3+ adsorbents are mostly studied, while those in the forms of lanthanum carbonate, La-MOF, and La-MOF derivatives are relatively few. The kinetic process of most phosphate adsorption is pseudo-second-order and the isotherm process is in accordance with the Langmuir model. The cost of La-based and other traditional adsorbents was compared. The adsorption mechanisms are categorized as electrostatic attraction, ligand exchange, Lewis acid-base interaction, ion exchange and surface precipitation. Besides, regeneration methods of La-based adsorbents are mainly acid, alkali, and salt-alkali. In addition, the La-based adsorbents after absorbing phosphate can be directly used as a slow-release fertilizer. This review provides a basis for the research on phosphate adsorption by La-based adsorbents. It should be carried out to further develop La-based materials with high adsorption capacity and good regeneration ability. Meanwhile, studies have been conducted on the reuse of phosphate after desorption, which needs more attention in future research.
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Lantano , Contaminantes Químicos del Agua , Adsorción , Álcalis , Concentración de Iones de Hidrógeno , Cinética , Fosfatos , FósforoRESUMEN
A novel vertical dual-loop reactor (VDLR) was developed to start and conduct a single-stage partial nitritation (PN) and anammox (PN/A) process for treating landfill leachate. Results showed that the total nitrogen (TN) removal reached 1.54 kg N/m3·d in the VDLR. It exhibited excellent mixing uniformity and buffer performance, which can increase the nitrogen removal performance up to 42.1 % via the improvement of anammox granular sludge activity (a particle size of 0.5-1 mm). Mass balance and microbial analysis indicated that the VDLR achieved efficient TN removal via anammox (99.24 %) and AOB (Nitrosomonas and Ellin6067) and anAOB (Candidatus kuenenia) played a vital role in this process.
RESUMEN
Soil lead pollution becomes a serious environmental problem. Microbial remediation has received widespread attentions due to high efficiency and no secondary pollution. In this research, a noval porous spherical phosphate-solubilizing bacteria bead loaded with biochar/nZVI (Bio-bead) was used to passivate lead in soil, and the effects and microecological regulation mechanisms of this process were also investigated. The results showed that the pH, OM, and AP of soil in the Bio-bead group were increased and the ORP was decreased over time compared with the blank group. The proportion of stable (oxidizable and residue) fractions of lead in Bio-bead group (45%) was much higher than that of the blank group (35%). In addition, the result of microbial community structure showed that Bio-beads did not change the species of dominant bacterial, excepting the abundance of Pseudomonas increased significantly and the abundance of Sphingomonas reduced during remediation. Redundancy analysis showed that pH, OM, AP and the ratio of residual and oxidizable fractions lead in soil were positively correlated with the abundance of Pseudomonas, while ORP was negatively correlated with the abundance of Pseudomonas. These findings have proved that Bio-bead is a potential strategy for remediation of lead-contaminated soil even in complexed soils.
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Restauración y Remediación Ambiental , Contaminantes del Suelo , Bacterias , Carbón Orgánico/química , Hierro/química , Plomo , Fosfatos , Porosidad , Suelo/química , Contaminantes del Suelo/análisisRESUMEN
Bioremediation technology has attracted increasing interest due to it efficient, economical and eco-friendly to apply to heavy metal contaminated soil. This study presents a new biological remediation system with phosphate functionalized iron-based nanomaterials and phosphate solubilizing bacterium strain Leclercia adecarboxylata. Different phosphate content functionalized iron-based nanomaterials were prepared, and nZVI@C/P1 (nP: nFe: nC=1:10:200) with high passivation efficiency was selected to combine with PSB for the remediation experiments. The change in lead fraction and microbial community under five conditions (CK, PSB, nZVI@C, nZVI@C/P1, nZVI@C/P1 + PSB) during 10 days incubation were investigate. The results indicated that nZVI@C/P1 + PSB increased the residual fraction of lead by 93.94% compared with the control group. Meanwhile, inoculation of Leclercia adecarboxylata became the dominant microflora in the soil microbial community during the remediation time, improving the utilization rate of phosphate in nZVI@C/P1 and enhancing the passivation efficiency of lead. Experimental findings demonstrated that combining nZVI@C/P1 with PSB could be considered as an efficient strategy for the lead contaminated soil remediation.
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Restauración y Remediación Ambiental , Nanoestructuras , Contaminantes del Suelo , Bacterias , Biodegradación Ambiental , Enterobacteriaceae , Hierro , Plomo , Fosfatos , Suelo , Contaminantes del Suelo/análisisRESUMEN
Phosphate solubilizing bacteria (PSBs) can effectively enhance the stability of lead via the formation of insoluble Pb-phosphate compounds. This research presents a bio-beads, which was implemented with the help of a self-designed porous spheres carrier, by immobilized PSBs strains Leclercia adecarboxylata (hereafter referred as L1-5). In addition, the passivation efficiency of lead via bio-beads under different conditions and its mechanism were also investigated in this study. The results indicated that phosphate solubilized by bio-beads could reach 30 mg/L in Ca3(PO4)2 medium containing 1 mM Pb2+, and the highest removal rate of Pb2+ in beef peptone liquid medium could reach 93%, which is better than that of free bacteria. Furthermore, it was also concluded that the lead could be transformed into stable crystal texture, such as Pb5(PO4)3Cl and Pb5(PO4)3OH. Both hydrophobic and hydrophilic groups in the bio-beads could capture Pb2+, which indicated that electrostatic attraction and ion-exchange were also the mechanism of Pb2+ adsorption. All the experimental findings demonstrated that this bio-bead could be consider as an efficient way for the lead immobilization in contaminated soil in the future.
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Carbón Orgánico/química , Enterobacteriaceae/metabolismo , Plomo/aislamiento & purificación , Contaminantes del Suelo/aislamiento & purificación , HierroRESUMEN
Phosphate solubilizing bacteria (PSBs) shows high potential to be used for lead passivation in sediments due to the abilities of releasing phosphate and the subsequent formation of insoluble Pb-phosphate compounds. In this research, microbial capsules implemented with sodium alginate and CaCl2, containing Leclercia adecarboxylata L15 (a lead resistant PSB) and Ca3(PO4)2, were developed and the performance on lead passivation under different conditions was examined. The optimal concentrations of sodium alginate and CaCl2 for formulating the capsules were determined to be 0.3% and 10%, respectively. The removal efficiency of Pb2+ by capsules containing L15 and Ca3(PO4)2 was up to 98% with a capsule dosage of 2%, initial Pb2+ concentration of 1mM and pH of 3.0, which was better than that of free L15 (18%) and capsules containing only L15 (34%). Lead was immobilized via the formation of Pb5(PO4)3Cl on the surface and Pb3(PO4)2 in the interior of the capsules. The simulated sediment remediation experiments showed that the acid soluble fraction of lead reduced from 28% to 14% and transformed into more stable fractions after 10 days. The experiment results indicated that PSBs capsules coupled with phosphate materials have a great promise for application in remediation of lead contaminated sediments.
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Fosfatos , Contaminantes del Suelo , Bacterias , Fosfatos de Calcio , Cápsulas , Enterobacteriaceae , Plomo , Fosfatos/análisis , Suelo , Contaminantes del Suelo/análisisRESUMEN
Phosphate solubilizing bacteria (PSB) can convert insoluble forms of phosphorus (P) to accessible forms. 11 strains of PSB, including five inorganic phosphate solubilizing bacteria (IPSBs) and six organic phosphate solubilizing bacteria (OPSBs), were isolated from rhizosphere soils of three plants Scirpus planiculmis, Zizania latifolia, and Phrnagmites australis in the Yeyahu Wetland of Beijing, China to investigate P-solubilizing activities. In addition, the distributions of P fractions in soil samples were also observed. All strains evaluated above 1.0 by the ratio of transparent circle diameter to colony diameter (D/d) on Ca3(PO4)2 or lecithin plates were identified by 16S rRNA sequencing. Results showed that Ca-bound P (Ca-P) was the main species of inorganic P (IP), and highly resistant organic P (HR-OP) accounted for the most part of organic P (OP). These strains were identified as bacterial species of Enterobacter asburiae, Acinetobacter sp., Bacillus cereus strain, and so on. The most efficient IPSB strain could convert over 430 mg L-1 orthophosphate, while the equivalent OPSB strain only liberated less than 4 mg L-1 in liquid culture, which indicated that IPSBs have a better P-solubilizing ability than OPSBs in rhizosphere soils of the Yeyahu Wetland and IPSBs are likely to regulate the P transformation process in this wetland. Graphical abstract á .
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Rizosfera , Microbiología del Suelo , Humedales , Beijing , China , Enterobacter , Organofosfatos , Fosfatos , Fósforo , Poaceae , ARN Ribosómico 16S/genética , SueloRESUMEN
The aim of this work was to ascertain the effects of Pb(II) and Cr(VI) on bacterial growth, generation of reactive oxygen species (ROS), activities of superoxide dismutase (SOD), and catalase (CAT), as well as the localization of bioaccumulated heavy metals in a phosphate-solubilizing bacterium. The results showed that the ROS increased from 1.4-fold to 1.8-fold of control under Pb(II) stress and decreased from 1.6-fold to 1.1-fold of control under Cr(VI) stress corresponding to metal concentrations (0.5-5 mmol·L-1). The SOD activities were ROS dependent; however, the CAT activities increased under both Pb(II) and Cr(VI) stress, from 11.4 to 21.8 U·mg-1 and 11.4 to 32.9 U·mg-1, respectively. Intra/extracellular accumulation were investigated by scanning transmission electron microscopy with energy dispersive X-ray spectroscopy (STEM-EDS) and it was calculated that extracellular accumulated Pb accounted for 61.7-95.9% of the total accumulation, while extracellular accumulated Cr only accounted for up to 3.6% of the total accumulation. Attenuated total reflection/Fourier-transform infrared spectroscopy (ATR-FTIR) analysis confirmed that the functional groups involved in those extracellular accumulation were not located in the loosely bound extracellular polysaccharides substances.
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Bacillus/crecimiento & desarrollo , Bacillus/metabolismo , Bioacumulación/efectos de los fármacos , Cromo/metabolismo , Plomo/metabolismo , Estrés Oxidativo/efectos de los fármacos , Fosfatos/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Catalasa/metabolismo , China , Metales Pesados/metabolismo , Oxidación-Reducción , Superóxido Dismutasa/metabolismoRESUMEN
Microorganisms in the rhizosphere of wetland plants can have a significant impact on phosphorus (P) interception. We investigated the seasonal pattern of microbial community structure and its relationship with different P forms in the rhizosphere of three plants Scirpus planiculmis, Zizania latifolia, and Phragmites australis from the Yeyahu Wetland, China. Chloroform fumigation-extraction was used to determine the soil microbial biomass P (SMBP) and phospholipid fatty acids (PLFA) were used to characterize microbial community composition. P fractions in rhizosphere soil samples were also observed using sequential chemical fractionation. Results showed that the average total PLFA (TPLFA) contents of rhizosphere soils ranged from 34.9 to 40.7nmol·g-1 and were highest in summer. Bacteria were predominant in the rhizospheres of all three plants, accounting for >63% of TPLFA. Aerobic bacteria, represented by 16:0 PLFA, were most abundant. Both organic P (OP) and inorganic P (IP) accumulated in the rhizosphere during the winter die-back phase. Furthermore, both TPLFA and bacterial PLFA decreased with increases in highly resistant OP (HR-OP), occluded P (Oc-P) and Calcium-bound P (Ca-P). This suggests that bacteria play an important role in P transformation and can make use of various P forms. We also found that SMBP was significantly negatively correlated with labile OP (L-OP), moderately labile OP (ML-OP) and HR-OP, reflecting a high degree of cross correlation between SMBP and the PLFA indices.
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Biodegradación Ambiental , Fósforo/metabolismo , Rizosfera , Microbiología del Suelo , Contaminantes del Suelo/metabolismo , Humedales , Suelo/químicaRESUMEN
In this research, microorganisms in rhizosphere/non-rhizosphere soils of Phragmites communis in the Yeyahu Wetland were studied. A sequential extraction procedure was used to analyze the phosphorus (P) forms in the rhizosphere/non-rhizosphere soil with a variety of plant growth conditions (April, July, October). The soil bacteria community structure and the diversity was measured using the high-throughput of 16S rRNA amplicons. Furthermore, the complete crystallographic analysis (CCA) method was used to analyze the relationship between phosphate solubilizing microorganisms and P transformation in the soil samples. The results showed that the rank order of inorganic P (IP) fractions in the soil was generally as follows:Ca-bound P (Ca-P) > Occluded P (Oc-P) > Fe-bound P (Fe-P) > Exchangeable P (Ex-P) > Al-bound P (Al-P). The IP content was most affected by the growth of Phragmites communis. The minimum content of IP appeared in the vigorous growth period and the total IP content in the rhizosphere soil was generally lower than in the non-rhizosphere soil. The rank order of organic P (OP) fractions were highly resistant OP (HR-OP) > moderately resistant OP (MR-OP) > moderately labile OP (ML-OP) > labile OP (L-OP), and all the components of OP first decreased and then increased with the growth of plant. The major phylogenic groups in rhizosphere/non-rhizosphere soil of Phragmites communis, included Proteobacteria, Acidobacteria, Chloroflexi, and Actinobacteria among which, Proteobacteria was the majority group in the community composition. Furthermore, the rhizosphere/non-rhizosphere microbial community structure was significantly affected by seasonal changes and existing differences between the rhizosphere and non-rhizosphere soils. In addition, the main functional groups of the modal transformation of P bacteria genera were Bacillus, Enterobacter, Pseudomonas, Burkholderia, Acinetobacter, which can make use of most OP and IP, playing an important role in the transformation of P in wetland soils.