Partner-switching components PmgA and Ssr1600 regulate high-light acclimation in Synechocystis sp. PCC 6803.

Photomixotrophic growth A (PmgA) is a pleiotropic regulator essential for growth under photomixotrophic and prolonged high-light (HL) conditions in the cyanobacterium Synechocystis sp. PCC 6803. The overall similarity with the antisigma factor of the bacterial partner-switching system indicates that PmgA exerts a regulatory function via phosphorylation of its target proteins. In this study, we performed an in vitro phosphorylation assay and protein-protein interaction analysis and found that PmgA interacts with 4 antisigma antagonist homologs, Ssr1600, Slr1856, Slr1859, and Slr1912, but specifically phosphorylates Ssr1600. Phenotypic analyses using the set of gene disruption and overexpression strains of pmgA and ssr1600 revealed that phosphorylation by PmgA is essential for the accumulation of Ssr1600 protein in vivo. The ssr1600-disrupted mutant showed similar phenotypes as those previously reported for the pmgA-disrupted mutant, namely, no obvious phenotype just after the shift to HL, but higher chlorophyll content, 5-aminolevulinic acid synthesis activity, and psaAB transcript levels than those in the wild type after 6 h. These findings indicate that the phosphorylated form of Ssr1600 works as the output of the partner-switching system to coordinately repress chlorophyll biosynthesis and accumulation of photosystem I during HL acclimation.

Tuning of ion-release capability from bio-ceramic-polymer composites for enhancing cellular activity.

In our previous study, we investigated the synergetic effects of inorganic ions, such as silicate, Mg2+ and Ca2+ ions on the osteoblast-like cell behaviour. Mg2+ ions play an important role in cell adhesion. In the present study, we designed a new composite that releases a high concentration of Mg2+ ions during the early stage of the bone-forming process, and silicate and Ca2+ ions continuously throughout this process. Here, 40SiO2-40MgO-20Na2O glass (G) with high solubility and vaterite-based calcium carbonate (V) were selected as the source of silicate and Mg2+ and Ca2+ ions, respectively. These particles were mixed with poly(lactic-co-glycolic acid) (PLGA) using a kneading method at 110°C to prepare the composite (G-V/PLGA, G/V/PLGA = 4/56/40 (in weight ratio)). Most of the Mg2+ ions were released within 3 days of immersion at an important stage for cell adhesion, and silicate and Ca2+ ions were released continuously at rates of 70-80 and 180 ppm d-1, respectively, throughout the experiment (until day 7). Mouse-derived osteoblast-like MC3T3-E1 proliferated more vigorously on G-V/PLGA in comparison with V-containing PLGA without G particles; it is possible to control the ion-release behaviour by incorporating a small amount of glass particles.