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1.
Proc Natl Acad Sci U S A ; 120(10): e2214076120, 2023 03 07.
Artículo en Inglés | MEDLINE | ID: mdl-36848567

RESUMEN

Lentinula is a broadly distributed group of fungi that contains the cultivated shiitake mushroom, L. edodes. We sequenced 24 genomes representing eight described species and several unnamed lineages of Lentinula from 15 countries on four continents. Lentinula comprises four major clades that arose in the Oligocene, three in the Americas and one in Asia-Australasia. To expand sampling of shiitake mushrooms, we assembled 60 genomes of L. edodes from China that were previously published as raw Illumina reads and added them to our dataset. Lentinula edodes sensu lato (s. lat.) contains three lineages that may warrant recognition as species, one including a single isolate from Nepal that is the sister group to the rest of L. edodes s. lat., a second with 20 cultivars and 12 wild isolates from China, Japan, Korea, and the Russian Far East, and a third with 28 wild isolates from China, Thailand, and Vietnam. Two additional lineages in China have arisen by hybridization among the second and third groups. Genes encoding cysteine sulfoxide lyase (lecsl) and γ-glutamyl transpeptidase (leggt), which are implicated in biosynthesis of the organosulfur flavor compound lenthionine, have diversified in Lentinula. Paralogs of both genes that are unique to Lentinula (lecsl 3 and leggt 5b) are coordinately up-regulated in fruiting bodies of L. edodes. The pangenome of L. edodes s. lat. contains 20,308 groups of orthologous genes, but only 6,438 orthogroups (32%) are shared among all strains, whereas 3,444 orthogroups (17%) are found only in wild populations, which should be targeted for conservation.


Asunto(s)
Lentinula , Filogenia , Asia Oriental , Tailandia
2.
World J Microbiol Biotechnol ; 28(3): 1315-9, 2012 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-22805853

RESUMEN

To establish a high throughput and cost-efficiency procedure for distinguishing among cultivars in Lentinula edodes, the polymorphism in the genes reported previously in this fungus was examined using PCR or PCR-RFLP techniques with a high-efficiency genome scanning (HEGS) system. As a result, PCR-based markers derived from eight genes (tyr, cap, ppa, IGS-RFLP, pri B-RFLP, mfbC-RFLP, gla-RFLP, xy-RFLP) showed polymorphisms among cultivars in this fungus and consequently, enabled to distinguish seventy-nine cultivars used in this study.


Asunto(s)
Marcadores Genéticos , Genoma Fúngico , Tipificación Molecular/métodos , Técnicas de Tipificación Micológica , Reacción en Cadena de la Polimerasa/métodos , Hongos Shiitake/clasificación , Hongos Shiitake/genética , Polimorfismo Genético , Polimorfismo de Longitud del Fragmento de Restricción
3.
Fungal Genet Biol ; 47(2): 152-8, 2010 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-19850145

RESUMEN

The fruiting-body primordium of Coprinopsis cinerea exhibits remarkable photomorphogenesis. Under a 12-h light/12-h dark regime, the primordium proceeds to the fruiting-body maturation phase in which the primordium successively undergoes basidiospore formation, stipe elongation and pileus expansion, resulting in the mature fruiting-body. In continuous darkness, however, the primordium never proceeds to the maturation phase: the pileus and stipe tissues at the upper part of the primordium remain rudimentary while the basal part of the primordium elongates, producing the etiolated "dark stipe" phenotype. In our previous studies, blind mutants, which produce dark stipes under light conditions that promote fruiting-body maturation in the wild-type, have been isolated, and two genes, dst1 and dst2, responsible for the mutant phenotype have been identified. In this study we show that the dst2-1 mutant exhibits a blind phenotype during asexual spore production in addition to that in fruiting-body photomorphogenesis. We also reveal that dst2 is predicted to encode a protein with a putative flavin adenine dinucleotide (FAD)-binding-4 domain. The two blind phenotypes, together with the existence of an FAD-binding domain in Dst2, suggest that Dst2 may play a role in perceiving blue light.


Asunto(s)
Coprinus/genética , Coprinus/metabolismo , Flavina-Adenina Dinucleótido/metabolismo , Proteínas Fúngicas/metabolismo , Northern Blotting , Clonación Molecular , Cuerpos Fructíferos de los Hongos/genética , Cuerpos Fructíferos de los Hongos/metabolismo , Proteínas Fúngicas/genética , Regulación Fúngica de la Expresión Génica , Modelos Genéticos , Mutación , Sistemas de Lectura Abierta , Fenotipo , Polimorfismo de Longitud del Fragmento de Restricción , Unión Proteica , Estructura Terciaria de Proteína
4.
Genetics ; 171(1): 101-8, 2005 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-15956671

RESUMEN

The homobasidiomycete Coprinus cinereus exhibits remarkable photomorphogenesis during fruiting-body development. Under proper light conditions, fruiting-body primordia proceed to the maturation phase in which basidia in the pileus undergo meiosis, producing sexual spores, followed by stipe elongation and pileus expansion for efficient dispersal of the spores. In the continuous darkness, however, the primordia do not proceed to the maturation phase but are etiolated: the pileus and stipe tissues at the upper part of the primordium remain rudimentary and the basal part of the primordium elongates, producing "dark stipe." In this study we genetically analyzed five strains that produce dark stipes even if light conditions promoting the maturation are given and then characterized one of them, Uar801 (dst1-1). The dst1 gene was cloned as a DNA fragment that rescues the dst1-1 mutation. Dst1 is predicted to be a protein of 1175 amino acids that contains two PAS domains, a coiled-coil structure, and a putative, glutamine-rich, transcriptional activation domain (AD). One of the PAS domains exhibits significant similarity to the LOV domains of known blue-light receptors, suggesting that Dst1 is a blue-light receptor of C. cinereus. The dst1-1 mutation is predicted to truncate the putative AD in the C-terminal region.


Asunto(s)
Coprinus/genética , Proteínas Fúngicas/genética , Alelos , Secuencia de Aminoácidos , Clonación Molecular , Coprinus/crecimiento & desarrollo , Coprinus/efectos de la radiación , ADN de Hongos/química , ADN de Hongos/genética , Regulación del Desarrollo de la Expresión Génica , Regulación Fúngica de la Expresión Génica , Luz , Datos de Secuencia Molecular , Morfogénesis/genética , Morfogénesis/efectos de la radiación , Mutación , Alineación de Secuencia , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido
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