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1.
Metab Brain Dis ; 31(6): 1249-1258, 2016 12.
Artículo en Inglés | MEDLINE | ID: mdl-27272740

RESUMEN

Hepatic encephalopathy (HE) is a major complication of liver failure/disease which frequently develops during the progression of end-stage liver disease. This metabolic neuropsychiatric syndrome involves a spectrum of symptoms, including cognition impairment, attention deficits and motor dysfunction which eventually can progress to coma and death. Pathologically, HE is characterized by swelling of the astrocytes which consequently leads to brain edema, a common feature found in patients with acute liver failure (ALF) as well as in cirrhotic patients suffering from HE. The pathogenic factors involved in the onset of astrocyte swelling and brain edema in HE are unresolved. However, the role of astrocyte swelling/brain edema in the development of HE remains ambiguous and therefore measuring brain edema as an endpoint to evaluate HE is questioned. The following review will determine the effect of astrocyte swelling and brain edema on neurological function, discuss the various possible techniques to measure brain edema and lastly to propose a number of neurobehavioral tests to evaluate HE.


Asunto(s)
Astrocitos/patología , Edema Encefálico/diagnóstico , Progresión de la Enfermedad , Determinación de Punto Final/normas , Encefalopatía Hepática/diagnóstico , Animales , Ansiedad/diagnóstico , Ansiedad/epidemiología , Ansiedad/psicología , Edema Encefálico/epidemiología , Edema Encefálico/psicología , Determinación de Punto Final/métodos , Encefalopatía Hepática/epidemiología , Encefalopatía Hepática/psicología , Humanos , Reproducibilidad de los Resultados
2.
Proc Natl Acad Sci U S A ; 108(2): 846-51, 2011 Jan 11.
Artículo en Inglés | MEDLINE | ID: mdl-21187412

RESUMEN

Aquaporin-4 (AQP4) is a primary influx route for water during brain edema formation. Here, we provide evidence that brain swelling triggers Ca(2+) signaling in astrocytes and that deletion of the Aqp4 gene markedly interferes with these events. Using in vivo two-photon imaging, we show that hypoosmotic stress (20% reduction in osmolarity) initiates astrocytic Ca(2+) spikes and that deletion of Aqp4 reduces these signals. The Ca(2+) signals are partly dependent on activation of P2 purinergic receptors, which was judged from the effects of appropriate antagonists applied to cortical slices. Supporting the involvement of purinergic signaling, osmotic stress was found to induce ATP release from cultured astrocytes in an AQP4-dependent manner. Our results suggest that AQP4 not only serves as an influx route for water but also is critical for initiating downstream signaling events that may affect and potentially exacerbate the pathological outcome in clinical conditions associated with brain edema.


Asunto(s)
Acuaporina 4/química , Acuaporina 4/genética , Astrocitos/metabolismo , Edema Encefálico/metabolismo , Calcio/metabolismo , Adenosina Trifosfato/química , Animales , Encéfalo/patología , Edema/patología , Femenino , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Ósmosis , Fotones , Transducción de Señal , Agua/química
4.
J Glaucoma ; 29(4): 280-286, 2020 04.
Artículo en Inglés | MEDLINE | ID: mdl-32053556

RESUMEN

PRéCIS:: Adjuvant diclofenac and apraclonidine eye drop given in conjunction with selective laser trabeculoplasty (SLT) do not significantly impact medium-term intraocular pressure (IOP) reduction compared with placebo, but apraclonidine can be used to blunt immediate postlaser pressure spikes. PURPOSE: There is limited high-grade evidence guiding the choice of eye drops given before and after SLT. The authors chose to measure IOP during the first 24 hours, at 1 week, 6 weeks, and 6 months after SLT, and compare the effect of apraclonidine before SLT and diclofenac after SLT, with placebo. MATERIALS AND METHODS: In this double-blind, randomized, placebo-controlled trial, patients with open-angle glaucoma or ocular hypertension referred for SLT were recruited between 2016 and 2018. Patients were randomized to receive either apraclonidine pre-SLT with placebo post-SLT, placebo pre-SLT with diclofenac post-SLT, or placebo before and after SLT. RESULTS: Sixty eyes from 35 patients were treated with 360-degree SLT. Twenty-four-hour IOP measurements with patient self-monitoring after SLT demonstrated a moderate IOP spike at 1 hour and 2 hours post-SLT in the placebo and diclofenac study arms (mean=+4.05±0.58 mm Hg and +4.47±0.73, respectively, P<0.001 vs. pre-SLT IOP), which was prevented by apraclonidine (mean=-2.41±0.88 mm Hg, P<0.0001 vs. other study arms post-SLT). There were no significant differences between the 3 arms of the study on the long-term IOP reduction achieved by SLT (6 wk: P=0.51, 6 mo: P=0.42). CONCLUSIONS: Neither the use of apraclonidine before SLT nor diclofenac after SLT significantly influenced the IOP reduction induced by SLT. Except for a slight and transient reduction in intraocular inflammation, there was no beneficial effect of diclofenac on early IOP changes or the degree of patient discomfort relative to placebo.


Asunto(s)
Clonidina/análogos & derivados , Diclofenaco/uso terapéutico , Glaucoma de Ángulo Abierto/cirugía , Presión Intraocular/efectos de los fármacos , Hipertensión Ocular/cirugía , Trabeculectomía , Agonistas de Receptores Adrenérgicos alfa 2/uso terapéutico , Anciano , Antiinflamatorios no Esteroideos/uso terapéutico , Clonidina/uso terapéutico , Método Doble Ciego , Femenino , Glaucoma de Ángulo Abierto/fisiopatología , Humanos , Terapia por Láser/efectos adversos , Láseres de Semiconductores/uso terapéutico , Masculino , Persona de Mediana Edad , Hipertensión Ocular/fisiopatología , Soluciones Oftálmicas/uso terapéutico , Tonometría Ocular
5.
Sci Transl Med ; 12(536)2020 03 25.
Artículo en Inglés | MEDLINE | ID: mdl-32213628

RESUMEN

Despite high metabolic activity, the retina and optic nerve head lack traditional lymphatic drainage. We here identified an ocular glymphatic clearance route for fluid and wastes via the proximal optic nerve in rodents. ß-amyloid (Aß) was cleared from the retina and vitreous via a pathway dependent on glial water channel aquaporin-4 (AQP4) and driven by the ocular-cranial pressure difference. After traversing the lamina barrier, intra-axonal Aß was cleared via the perivenous space and subsequently drained to lymphatic vessels. Light-induced pupil constriction enhanced efflux, whereas atropine or raising intracranial pressure blocked efflux. In two distinct murine models of glaucoma, Aß leaked from the eye via defects in the lamina barrier instead of directional axonal efflux. The results suggest that, in rodents, the removal of fluid and metabolites from the intraocular space occurs through a glymphatic pathway that might be impaired in glaucoma.


Asunto(s)
Sistema Glinfático , Péptidos beta-Amiloides/metabolismo , Animales , Acuaporina 4/metabolismo , Sistema Glinfático/metabolismo , Presión Intracraneal , Ratones , Nervio Óptico , Retina , Cuerpo Vítreo
6.
Elife ; 72018 12 18.
Artículo en Inglés | MEDLINE | ID: mdl-30561329

RESUMEN

The glymphatic system is a brain-wide clearance pathway; its impairment contributes to the accumulation of amyloid-ß. Influx of cerebrospinal fluid (CSF) depends upon the expression and perivascular localization of the astroglial water channel aquaporin-4 (AQP4). Prompted by a recent failure to find an effect of Aqp4 knock-out (KO) on CSF and interstitial fluid (ISF) tracer transport, five groups re-examined the importance of AQP4 in glymphatic transport. We concur that CSF influx is higher in wild-type mice than in four different Aqp4 KO lines and in one line that lacks perivascular AQP4 (Snta1 KO). Meta-analysis of all studies demonstrated a significant decrease in tracer transport in KO mice and rats compared to controls. Meta-regression indicated that anesthesia, age, and tracer delivery explain the opposing results. We also report that intrastriatal injections suppress glymphatic function. This validates the role of AQP4 and shows that glymphatic studies must avoid the use of invasive procedures.


Asunto(s)
Acuaporina 4/metabolismo , Astrocitos/metabolismo , Encéfalo/metabolismo , Sistema Glinfático , Animales , Acuaporina 4/genética , Transporte Biológico , Líquido Cefalorraquídeo/metabolismo , Líquido Extracelular/metabolismo , Ratones Noqueados , Ratas
8.
Trends Neurosci ; 37(11): 620-8, 2014 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-25236348

RESUMEN

Edema formation frequently complicates brain infarction, tumors, and trauma. Despite the significant mortality of this condition, current treatment options are often ineffective or incompletely understood. Recent studies have revealed the existence of a brain-wide paravascular pathway for cerebrospinal (CSF) and interstitial fluid (ISF) exchange. The current review critically examines the contribution of this 'glymphatic' system to the main types of brain edema. We propose that in cytotoxic edema, energy depletion enhances glymphatic CSF influx, whilst suppressing ISF efflux. We also argue that paravascular inflammation or 'paravasculitis' plays a critical role in vasogenic edema. Finally, recent advances in diagnostic imaging of glymphatic function may hold the key to defining the edema profile of individual patients, and thus enable more targeted therapy.


Asunto(s)
Astrocitos/patología , Edema Encefálico/patología , Encéfalo/metabolismo , Líquido Cefalorraquídeo/metabolismo , Líquido Extracelular/metabolismo , Animales , Astrocitos/metabolismo , Barrera Hematoencefálica/metabolismo , Barrera Hematoencefálica/patología , Encéfalo/patología , Edema Encefálico/metabolismo , Humanos
10.
Sci Rep ; 3: 2582, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-24002448

RESUMEN

In the brain, a paravascular space exists between vascular cells and astroglial end-foot processes, creating a continuous sheath surrounding blood vessels. Using in vivo two-photon imaging we demonstrate that the paravascular circulation facilitates selective transport of small lipophilic molecules, rapid interstitial fluid movement and widespread glial calcium signaling. Depressurizing the paravascular system leads to unselective lipid diffusion, intracellular lipid accumulation and pathological signaling in astrocytes. As the central nervous system is devoid of lymphatic vessels, the paravascular space may serve as a lymphatic equivalent that represents a separate highway for the transport of lipids and signaling molecules.


Asunto(s)
Astrocitos/metabolismo , Encéfalo/irrigación sanguínea , Encéfalo/metabolismo , Metabolismo de los Lípidos , Microcirculación , Transducción de Señal , Animales , Transporte Biológico , Calcio/metabolismo , Femenino , Colorantes Fluorescentes/administración & dosificación , Colorantes Fluorescentes/química , Masculino , Ratones , Ratones Transgénicos , Microscopía Confocal , Imagen Molecular/métodos
11.
Cell Calcium ; 54(6): 387-94, 2013 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-24138901

RESUMEN

Astrocyte Ca2+ signals in awake behaving mice are widespread, coordinated and differ fundamentally from the locally restricted Ca2+ transients observed ex vivo and in anesthetized animals. Here we show that the synchronized release of norepinephrine (NE) from locus coeruleus (LC) projections throughout the cerebral cortex mediate long-ranging Ca2+ signals by activation of astrocytic α1-adrenergic receptors. When LC output was triggered by either physiological sensory (whisker) stimulation or an air-puff startle response, astrocytes responded with fast Ca2+ transients that encompassed the entire imaged field (positioned over either frontal or parietal cortex). The application of adrenergic inhibitors, including α1-adrenergic antagonist prazosin, potently suppressed both evoked, as well as the frequently observed spontaneous astroglial Ca2+ signals. The LC-specific neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP-4), which reduced cortical NE content by >90%, prevented nearly all astrocytic Ca2+ signals in awake mice. The observations indicate that in adult, unanesthetized mice, astrocytes do not respond directly to glutamatergic signaling evoked by sensory stimulation. Instead astrocytes appear to be the primary target for NE, with astrocytic Ca2+ signaling being triggered by the α1-adrenergic receptor. In turn, astrocytes may coordinate the broad effects of neuromodulators on neuronal activity.


Asunto(s)
Astrocitos/metabolismo , Señalización del Calcio , Receptores Adrenérgicos alfa 1/metabolismo , Agonistas de Receptores Adrenérgicos alfa 1/farmacología , Antagonistas de Receptores Adrenérgicos alfa 1/farmacología , Animales , Conducta Animal/efectos de los fármacos , Bencilaminas/farmacología , Corteza Cerebral/efectos de los fármacos , Corteza Cerebral/metabolismo , Locus Coeruleus/efectos de los fármacos , Locus Coeruleus/metabolismo , Ratones , Ratones Endogámicos C57BL , Inhibidores de la Captación de Neurotransmisores/farmacología , Norepinefrina/metabolismo , Prazosina/farmacología , Receptores Adrenérgicos alfa 1/química
12.
J Cereb Blood Flow Metab ; 33(7): 996-9, 2013 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-23611872

RESUMEN

Using in vivo two-photon imaging, we show that mice deficient in aquaporin-4 (AQP4) display increased fluorescence of nicotinamide adenine dinucleotide (NADH) when subjected to cortical spreading depression. The increased NADH signal, a proxy of tissue hypoxia, was restricted to microwatershed areas remote from the vasculature. Aqp4 deletion had no effects on the hyperemia response, but slowed [K(+)]o recovery. These observations suggest that K(+) uptake is suppressed in Aqp4(-/-) mice as a consequence of decreased oxygen delivery to tissue located furthest away from the vascular source of oxygen, although increased oxygen consumption may also contribute to our observations.


Asunto(s)
Acuaporina 4/fisiología , Depresión de Propagación Cortical/fisiología , Hipoxia Encefálica/metabolismo , Microscopía de Fluorescencia por Excitación Multifotónica/métodos , NAD/metabolismo , Oxígeno/metabolismo , Animales , Acuaporina 4/genética , Circulación Cerebrovascular/efectos de los fármacos , Circulación Cerebrovascular/fisiología , Depresión de Propagación Cortical/efectos de los fármacos , Depresión de Propagación Cortical/genética , Femenino , Eliminación de Gen , Masculino , Ratones , Ratones Noqueados , Modelos Biológicos , NAD/química , Consumo de Oxígeno/fisiología , Cloruro de Potasio/metabolismo , Cloruro de Potasio/farmacología
13.
Nat Med ; 19(12): 1643-8, 2013 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-24240184

RESUMEN

Ammonia is a ubiquitous waste product of protein metabolism that can accumulate in numerous metabolic disorders, causing neurological dysfunction ranging from cognitive impairment to tremor, ataxia, seizures, coma and death. The brain is especially vulnerable to ammonia as it readily crosses the blood-brain barrier in its gaseous form, NH3, and rapidly saturates its principal removal pathway located in astrocytes. Thus, we wanted to determine how astrocytes contribute to the initial deterioration of neurological functions characteristic of hyperammonemia in vivo. Using a combination of two-photon imaging and electrophysiology in awake head-restrained mice, we show that ammonia rapidly compromises astrocyte potassium buffering, increasing extracellular potassium concentration and overactivating the Na(+)-K(+)-2Cl(-) cotransporter isoform 1 (NKCC1) in neurons. The consequent depolarization of the neuronal GABA reversal potential (EGABA) selectively impairs cortical inhibitory networks. Genetic deletion of NKCC1 or inhibition of it with the clinically used diuretic bumetanide potently suppresses ammonia-induced neurological dysfunction. We did not observe astrocyte swelling or brain edema in the acute phase, calling into question current concepts regarding the neurotoxic effects of ammonia. Instead, our findings identify failure of potassium buffering in astrocytes as a crucial mechanism in ammonia neurotoxicity and demonstrate the therapeutic potential of blocking this pathway by inhibiting NKCC1.


Asunto(s)
Amoníaco/farmacología , Astrocitos/efectos de los fármacos , Inhibición Neural/efectos de los fármacos , Neuronas/efectos de los fármacos , Potasio/metabolismo , Convulsiones/inducido químicamente , Animales , Animales Recién Nacidos , Astrocitos/metabolismo , Células Cultivadas , Evaluación Preclínica de Medicamentos , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Neuronas/fisiología , Ratas , Ratas Wistar , Convulsiones/fisiopatología
14.
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