RESUMEN
Adenoviruses are generally weak interferon inducers, triggering chicken embryo fibroblast cells by a UV-resistant viral component, probably the capsid or capsid elements, to produce 50 to 100 IU of interferon per ml. Adenovirus types 12, 18, and 31, however, can induce by a UV-sensitive mechanism 10 to 20 times more interferon than other types do. By using mutant and recombinant adenoviruses, we demonstrated that early region 1A was responsible for the enhanced interferon production of chicken cells infected with adenovirus type 12.
Asunto(s)
Adenoviridae/fisiología , Interferón Tipo I/biosíntesis , Proteínas Oncogénicas Virales/fisiología , Adenoviridae/clasificación , Adenoviridae/genética , Proteínas Precoces de Adenovirus , Animales , Cápside/fisiología , Cápside/efectos de la radiación , Embrión de Pollo , Fibroblastos/metabolismo , Recombinación Genética , Transcripción Genética , Rayos UltravioletaRESUMEN
Human interferon-alpha 1 and interferon-beta genes with their flanking regions were introduced into mouse LMTK- cells. Although transfected cells contained the interferon genes with a similar copy number and produced a similar amount of interferon-specific mRNA, cells containing the human interferon-beta gene secreted about 10 times more human interferon than cells transfected with the human interferon-alpha 1 gene. When the coding region of the interferon-beta gene was replaced by that of the interferon-alpha 1 gene (hybrid interferon beta/alpha gene), the human interferon production of transfected cells fell by approx. one order of magnitude. These results show that in the case of exogenous interferon genes a translational or post-translational mechanism might significantly affect the final level of human interferons, resulting in higher titres of interferon-beta than of interferon-alpha.