RESUMEN
Three new pyrrole oligoglycosides, astebatheriosides A-C (1-3), and a new furan oligoglycoside, astebatherioside D (4), were isolated from the starfish Asterina batheri by various chromatographic methods. Their structures were elucidated by spectroscopic and chemical methods. Compounds 2, 3, and 4 moderately inhibited IL-12 p40 production in lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells (BMDCs) with IC50 values of 36.4, 31.6, and 22.8µM, respectively.
Asunto(s)
Asterina/química , Citocinas/metabolismo , Células Dendríticas/metabolismo , Glicósidos/química , Animales , Células de la Médula Ósea/citología , Células Cultivadas , Células Dendríticas/citología , Células Dendríticas/efectos de los fármacos , Furanos/química , Glicósidos/aislamiento & purificación , Glicósidos/farmacología , Subunidad p40 de la Interleucina-12/metabolismo , Interleucina-6/metabolismo , Lipopolisacáridos/toxicidad , Espectroscopía de Resonancia Magnética , Conformación Molecular , Pirroles/química , Factor de Necrosis Tumoral alfa/metabolismoRESUMEN
Two new guaiane derivatives (1 and 2) along with six known sesquiterpenoids (3-8) were isolated from the gorgonian Menella woodin. Their structures -were elucidated by ID and 2D NMR and HRESIMS data as well as by comparison of their spectra with those in the literature. Relative configurations of asymmetric centers in 1 and 2 were suggested on the basis of NOESY and ID NOE correlations, absolute stereochemistry of these compounds was proposed in result of comparison of calculated (for both enatiomers) and experimental ECD. Some suggestions were made regarding a biosynthesis of guaiane sesquiterpenoids in this species. All the compounds were firstly isolated from M woodin.
Asunto(s)
Antozoos/química , Sesquiterpenos/química , Animales , Estructura MolecularRESUMEN
Nine secondary metabolites, including a new cycloartane glucoside, rhizostyloside (1), were isolated from a methanol extract of Rhizophora stylosa leaves through several chromatographic experiments. The structures of the compounds were determined on the basis of NMR spectroscopic (1H and 13C NMR, HSQC, HMBC, 1H-1H COSY, NOESY) and HR-ESI-MS data and by comparison with literature values. Compound 1 exhibited significant cytotoxicity against three human cancer cell lines: KB (epidermoid carcinoma), LU-1 (lung adenocarcinoma), and SK-Mel-2 (melanoma). In addition, 1 strongly activated caspase-3/7 in LU-1 cells.