Asunto(s)
Gastritis , Hiperpotasemia/tratamiento farmacológico , Poliestirenos , Sorbitol , Estómago , Lesión Renal Aguda/complicaciones , Anciano , Catárticos/administración & dosificación , Catárticos/efectos adversos , Quelantes/administración & dosificación , Quelantes/efectos adversos , Gastritis/inducido químicamente , Gastritis/diagnóstico , Gastroscopía/métodos , Humanos , Hiperpotasemia/etiología , Isquemia/inducido químicamente , Masculino , Necrosis/inducido químicamente , Poliestirenos/administración & dosificación , Poliestirenos/efectos adversos , Sorbitol/administración & dosificación , Sorbitol/efectos adversos , Estómago/irrigación sanguínea , Estómago/diagnóstico por imagen , Estómago/patología , Tomografía Computarizada por Rayos X/métodosAsunto(s)
Esófago/irrigación sanguínea , Neoplasias Gastrointestinales/complicaciones , Nevo Azul/complicaciones , Neoplasias Cutáneas/complicaciones , Enfermedades Vasculares/etiología , Esófago/patología , Neoplasias Gastrointestinales/patología , Humanos , Masculino , Persona de Mediana Edad , Nevo Azul/patología , Neoplasias Cutáneas/patología , Enfermedades Vasculares/patologíaAsunto(s)
Leiomiosarcoma/secundario , Neoplasias Retroperitoneales/patología , Neoplasias Gástricas/secundario , Adulto , Femenino , Humanos , Leiomiosarcoma/diagnóstico por imagen , Pólipos/diagnóstico por imagen , Neoplasias Retroperitoneales/diagnóstico por imagen , Neoplasias Gástricas/diagnóstico por imagen , Tomografía Computarizada por Rayos XRESUMEN
Glial cell line-derived neurotrophic factor (GDNF) is a factor produced by glial cells that is required for the development of the enteric nervous system. In transgenic mice that overexpress GDNF in the pancreas, GDNF has been shown to enhance beta-cell mass and improve glucose control, but the transcriptional and cellular processes involved are not known. In this study we examined the influence of GDNF on the expression of neurogenin3 (Ngn3) and other transcription factors implicated in early beta-cell development, as well as on beta-cell proliferation during embryonic and early postnatal mouse pancreas development. Embryonic day 15.5 (E15.5) mouse pancreatic tissue when exposed to GDNF for 24 h showed higher Ngn3, pancreatic and duodenal homeobox gene 1 (Pdx1), neuroD1/beta(2), paired homeobox gene 4 (Pax4), and insulin mRNA expression than tissue exposed to vehicle only. Transgenic expression of GDNF in mouse pancreata was associated with increased numbers of Ngn3-expressing pancreatic cells and higher beta-cell mass at embryonic day 18 (E18), as well as higher beta-cell proliferation and Pdx1 expression in beta-cells at E18 and postnatal day 1. In the HIT-T15 beta-cell line, GDNF enhanced the expression of Pax6. This response was, however, blocked in the presence of Pdx1 small interfering RNA (siRNA). Chromatin immunoprecipitation studies using the HIT-T15 beta-cell line demonstrated that GDNF can influence Pdx1 gene expression by enhancing the binding of Sox9 and neuroD1/beta(2) to the Pdx1 promoter. Our data provide evidence of a mechanism by which GDNF influences beta-cell development. GDNF could be a potential therapeutic target for the treatment and prevention of diabetes.
Asunto(s)
Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/metabolismo , Proliferación Celular , Factor Neurotrófico Derivado de la Línea Celular Glial/metabolismo , Células Secretoras de Insulina/metabolismo , Proteínas del Tejido Nervioso/metabolismo , Páncreas/metabolismo , Animales , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/genética , Sitios de Unión , Línea Celular , Inmunoprecipitación de Cromatina , Cricetinae , Proteínas del Ojo/metabolismo , Regulación del Desarrollo de la Expresión Génica , Edad Gestacional , Factor Neurotrófico Derivado de la Línea Celular Glial/genética , Proteínas de Homeodominio/metabolismo , Ratones , Ratones Transgénicos , Proteínas del Tejido Nervioso/genética , Organogénesis , Factor de Transcripción PAX6 , Factores de Transcripción Paired Box/metabolismo , Páncreas/embriología , Regiones Promotoras Genéticas , Interferencia de ARN , ARN Mensajero/metabolismo , Ratas , Proteínas Recombinantes/metabolismo , Proteínas Represoras/metabolismo , Factor de Transcripción SOX9/metabolismo , Transactivadores/metabolismo , Factor de Transcripción HES-1 , Activación Transcripcional , Transfección , Regulación hacia ArribaAsunto(s)
Absceso Abdominal/complicaciones , Absceso Abdominal/diagnóstico , Drenaje , Endoscopía del Sistema Digestivo , Obstrucción de la Salida Gástrica/etiología , Absceso Abdominal/patología , Absceso Abdominal/cirugía , Adulto , Femenino , Obstrucción de la Salida Gástrica/cirugía , Humanos , Cavidad Peritoneal , Resultado del TratamientoAsunto(s)
Dolor Abdominal , Dentaduras , Cuerpos Extraños , Válvula Ileocecal/patología , Dolor Abdominal/diagnóstico , Dolor Abdominal/etiología , Dolor Abdominal/cirugía , Anciano , Colonoscopía/métodos , Cuerpos Extraños/complicaciones , Cuerpos Extraños/diagnóstico , Cuerpos Extraños/fisiopatología , Cuerpos Extraños/cirugía , Humanos , Masculino , Tomografía Computarizada por Rayos X , Resultado del TratamientoRESUMEN
We present a 24-year-old combat veteran who underwent extensive work-up for elevated aminotransferases, including liver biopsy, with no underlying pathology identified. Subsequent investigations showed elevated creatinine kinase and aldolase. The patient was later diagnosed with biopsy-proven dysferlin-deficient muscular dystrophy. Persistent transaminase elevation despite negative liver work-up should prompt clinicians to consider extrahepatic sources of enzyme elevation. Promptly correlating aminotransferase elevation with musculoskeletal pathology may present an opportunity for clinicians to detect myopathies such as muscular dystrophy in their preclinical stages.
RESUMEN
BACKGROUND: Development of pretransplantation islet culture strategies that preserve or enhance ß-cell viability would eliminate the requirement for the large numbers of islets needed to restore insulin independence in type 1 diabetes patients. We investigated whether glial cell line-derived neurotrophic factor (GDNF) could improve human islet survival and posttransplantation function in diabetic mice. METHODS: Human islets were cultured in medium supplemented with or without GDNF (100 ng/mL) and in vitro islet survival and function assessed by analyzing ß-cell apoptosis and glucose stimulated insulin release. In vivo effects of GDNF were assessed in streptozotocin-induced diabetic nude mice transplanted under the kidney capsule with 2000 islet equivalents of human islets precultured in medium supplemented with or without GDNF. RESULTS: In vitro, human islets cultured for 2 to 10 days in medium supplemented with GDNF showed lower ß-cell death, increased Akt phosphorylation, and higher glucose-induced insulin secretion than islets cultured in vehicle. Human islets precultured in medium supplemented with GDNF restored more diabetic mice to normoglycemia and for a longer period after transplantation than islets cultured in vehicle. CONCLUSIONS: Our study shows that GDNF has beneficial effects on human islet survival and could be used to improve islet posttransplantation survival.