A dust-enshrouded tidal disruption event with a resolved radio jet in a galaxy merger.

Tidal disruption events (TDEs) are transient flares produced when a star is ripped apart by the gravitational field of a supermassive black hole (SMBH). We have observed a transient source in the western nucleus of the merging galaxy pair Arp 299 that radiated >1.5 × 1052 erg at infrared and radio wavelengths but was not luminous at optical or x-ray wavelengths. We interpret this as a TDE with much of its emission reradiated at infrared wavelengths by dust. Efficient reprocessing by dense gas and dust may explain the difference between theoretical predictions and observed luminosities of TDEs. The radio observations resolve an expanding and decelerating jet, probing the jet formation and evolution around a SMBH.

Regulation of transcription from the human muscle phosphofructokinase P2 promoter by the Sp1 transcription factor.

The human muscle phosphofructokinase (HPFKM) p2 promoter contains sequence elements that are similar to the Sp1 transcription factor binding site consensus sequence. DNase I footprinting identified four regions of the HPFKM p2 promoter that bound purified Sp1. Gel retardation analysis using HeLa S3 nuclear extracts and purified Sp1 protein demonstrated that each of the four recognition elements bound the Sp1 transcription factor. The function of the HPFKM p2 promoter elements was examined in transient transfection assays using these binding sites cloned into a minimal promoter element. In Drosophila Schneider line-2 cells, each of these regulatory regions trans-activated transcription from a minimal promoter element in response to exogenously expressed Sp1. In addition, transcription from the HPFKM p2 promoter was shown to be trans-activated by exogenously expressed Sp1 in Drosophila Schneider line-2 cells. Deletion analysis of the HPFKM p2 promoter demonstrated that the promoter region between -66 and +16 was sufficient to confer sp1 responsiveness. This promoter region includes one of the regulatory elements footprinted by the purified Sp1 transcription factor and mediates the majority of the transcriptional activity from the HPFKM p2 promoter in the human cervical carcinoma cell line HeLa S3. This demonstrates that the HPFKM p2 promoter contains four functional Sp1 binding sites that may contribute to the level of transcription from this promoter in a variety of cell types.

Cloning and characterization of the human muscle phosphofructokinase gene.

A 35-kbp region of genomic DNA encoding the human muscle phosphofructokinase (HPFK-M) gene including all of the coding exons (1-22) plus 2.2-kbp of 5'-flanking sequence has been cloned. The exon boundaries are the same as has been observed for the rabbit muscle phosphofructokinase (RPFK-M), the human liver phosphofructokinase (HPFK-L), and the mouse liver phosphofructokinase (MPFK-L) genes. Characterization of the structure of the HPFK-M gene and its transcript in Epstein-Barr virus transformed B-cell lines derived from patients with glycogen storage disease type VII (GSDVII or Tarui's disease) demonstrated that this single-copy gene encodes a normal sized 3.0-kb transcript in the four cases examined. This suggests the lesion in these cases represents either a point mutation or possibly a small insertion or deletion resulting in the synthesis of a defective HPFK-M protein. Analysis of the 5'-flanking region demonstrated the presence of a functional promoter located within 114 nucleotides of a proposed transcription initiation site. This promoter was active in the human cervical carcinoma cell line, HeLa S3, the dedifferentiated human hepatoma cell line, HepG2.1, and the mouse myoblast cell line, C2C12, suggesting this promoter has a broad cell-type specificity. In addition, from the known HPFK-M cDNA sequences, this observation indicates that the HPFK-M gene has a second promoter located upstream from the genomic region isolated in this study.

Simultaneous detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis by a rapid PCR method.

The identification of periodontal pathogens by conventional methods is time-consuming and difficult. Therefore, a multiplex PCR method for simultaneous detection of Actinobacillus actinomycetemcomitans (A.a.) and Porphyromonas gingivalis (P.g.) was developed for rapid and easy determination of these risk-indicator bacteria in human periodontal disease. The PCR primers were designed to hybridize to various regions of 16S rRNA genes, and a hot-start technique was used to obtain maximum sensitivity and specificity. This method can detect both of these bacteria in subgingival plaque samples at concentrations as low as 5 to 50 cells per sample. The sensitivity, however, was even 10 times better when the bacteria were analyzed in a water suspension. Since the only step between sample collection and the actual analysis is a brief centrifugation of the patient sample, the detection can be readily carried out in four hours. The performance of the method was studied with 36 patient samples. The results showed that the PCR method detected A.a. (44% vs. 25%, respectively) and P.g. (56% vs. 42%, respectively) more often than the conventional culture in plaque samples. Thus, our multiplex PCR method is rapid and more effective than conventional protocols in detecting these periodontal pathogens.

Identification of Bacteroides forsythus in subgingival dental plaque with the aid of a rapid PCR method.

Bacteroides forysthus has been shown to be prevalent among patients with periodontitis. Conventional microbiological methods used to identify this bacterium, however, are laborious and time-consuming and are therefore not well-suited for screening purposes. We have developed a polymerase chain-reaction (PCR) method which is rapid, specific, and simple to perform and does not require other sample pre-treatment except a brief centrifugation. This method was applied to the detection of B. forsythus in subgingival plaque of 58 periodontitis patients. When compared with the results of conventional culturing, the PCR method always confirmed the culture-positive results, while none of the PCR negative samples was shown to be culture-positive. The PCR method appeared to give more than double the number of samples positive for B. forsythus than culturing (89.7% vs. 37.9%). The analysis requires less than 4 hrs to perform, and is specific only to B. forsythus and sensitive enough to detect fewer than 5 bacteria.

Periodontal health in 45,X women (Turner's syndrome).

The overall periodontal states of 81 women with 45,X chromosome complement (Turner's Syndrome) and a sister of 31 of these women were studied. The ages of the subjects ranged from 13 to 35 years. Mean Gingival Index and Calculus Index values were lower in all 45,X women studied and in a subgroup of 45,X women whose sisters formed the control group than in the controls. When the effect of age was taken into account differences were similar. Pathologically deep probing depths of 4 mm or over were found in 9 (11.1%) of all 45,X women, in 3 (9.7%) of the subgroup of 45,X women whose sisters formed the control group and in 7 (22.6%) of the controls. The results indicate that periodontal health was better in the 45,X females than in the controls.

Periodontal health in 47,XXY men (Klinefelter syndrome).

Periodontal health in 37 patients with a 47,XXY chromosome complement (Klinefelter Syndrome) and in 12 brothers of patients was studied. Mean age-adjusted Gingival, Gingival Bleeding and Calculus Indices were statistically significantly higher in 47,XXY men than in the controls. The mean percentage of tooth surfaces with pathologically deepened probing depths for all 47,XXY men was 8.5%, for the subgroup of 47,XXY men with brothers it was 7.1%, and for the brothers it was 7.5%. Tooth mobility was found in two 47,XXY men and in one control. The results indicate that gingival inflammation was increased in the 47,XXY men than in the controls.

Measurement of gas exchange in intensive care: laboratory and clinical validation of a new device.

The performance of a new gas exchange monitor was assessed both in laboratory simulation and in ICU patients. Laboratory simulation using N2 and CO2 injections resulted in a mean error of 2 +/- 2% in CO2 production (VCO2) and 4 +/- 4% in oxygen consumption (VO2) in respirator measurements (n = 55) and in a mean error of 3 +/- 2% in VCO2 and 4 +/- 2% in VO2 in canopy measurements (n = 25). The mean error in RQ during ethanol burning was 2 +/- 2% in respirator measurements (n = 45) and 1 +/- 1% in canopy measurements. FIO2 had little effect on the accuracy of VCO2, whereas the accuracy on high rates of VO2 (VO2 = 400 ml/min) was reduced, when FIO2 increased: the error ranged from 1 +/- 1% to 6 +/- 1%, except at VO2 400 ml/min during FIO2 0.8, where the error was 16 +/- 3%. Neither peak airway pressure (+13 to +63 cm H2O) nor PEEP (0 to +20 cm H2O) had an effect on the accuracy. The highest level of minute ventilation studied (22.5 L/min) reduced the accuracy slightly (mean error of VCO2 4 +/- 1% and VO2 7 +/- 2%). In patients during controlled mechanical ventilation, increasing FIO2 from 0.4 to 0.6 had no effect on the results. VO2 was consistently higher by gas exchange than by the Fick principle: 16 +/- 9% during controlled ventilation (n = 20), 21 +/- 8% on synchronized intermittent mandatory ventilation (n = 10) and 25 +/- 8% during spontaneous breathing. We conclude that the device proved to be accurate for gas exchange measurements in the ICU.

Polymorphic restriction sites of type II collagen gene: their location and frequencies in the Finnish population.

Restriction fragment length polymorphism (RFLP) of the cartilage-specific type II collagen gene has been studied in the Finnish population. Two high-frequency alleles, also reported in other populations, were detected. The HindIII allele had a frequency of 0.33, and that detected with PvuII a frequency of 0.46. Both of these frequencies resembled the ones reported for other populations. Also one BamHI allele, not earlier reported, was found at a low frequency. Two other previously reported polymorphisms for BamHI and EcoRI were not detected in the Finnish population. The RFLPs showed a fair agreement with the Hardy-Weinberg equilibrium. A linkage disequilibrium was found between PvuII and HindIII markers. The alpha 1(II) collagen gene seems to be more conserved in populations of various origins than the alpha 2(I) collagen gene. These polymorphic collagen markers would be useful in linkage studies of various inherited cartilage disorders.

Predisposition to familial osteoarthrosis linked to type II collagen gene.

The genetic background of two families, in whom a predisposition to primary osteoarthrosis is inherited as a dominant trait, was investigated. Use of restriction fragment length polymorphisms within and around the type II collagen gene on chromosome 12 revealed a linkage between this cartilage-specific gene and primary osteoarthrosis.