RESUMEN
Here, we describe 4-dimethylaminoantipyrine (4-DMAA)-mediated interfacing as a broad biochemical indicator to stabilize and promote the higher response of electrodes for immunological detection. We hypothesized that the improved biological interactions of 4-DMAA with electrodes and biological samples may be due to the interaction properties of the benzene and pyrazole chemical groups with graphite and proteins, respectively. In order to demonstrate that 4-DMAA could be used as a general indicator in electrochemical immunoassays, we used peptides as probes for the diagnosis of four neglected tropical infectious diseases Tegumentary leishmaniasis, Visceral leishmaniasis, Strongyloidiasis, and Leprosy on commercial graphite screen-printed electrodes. 4-DMAA oxidation was used to indicate specific biological recognition between the epitope-based peptide and serum immunoglobulin G (IgG) from infected patients. We demonstrated that 4-DMAA should be incorporated into the electrodes prior to serum application, which avoids interference with its sensitivity and specificity. In addition, 4-DMAA oxidizes at a low anodic potential, and the oxidation peak is useful for detecting proteins in biological fluids. In summary, we have successfully demonstrated the broad application of 4-DMAA as a general indicator for the specific diagnosis of four infectious diseases in electrochemical immunosensors. Such a strategy is quite advantageous for indirect detection of proteins that lack electrochemical activities or are spatially inaccessible on the electrode surface. This new indicator opens a new avenue for monitoring biological recognition, especially for immunosensors.
Asunto(s)
Técnicas Biosensibles , Grafito , Aminopirina , Electrodos , Humanos , InmunoensayoRESUMEN
Glyphosate detection and quantification is still a challenge. After an extensive review of the literature, we observed that Fourier transform infrared spectroscopy (FTIR) had practically not yet been used for detection or quantification. The interaction between zinc oxide (ZnO), silver oxide (Ag2O), and Ag-doped ZnO nanocrystals (NCs), as well as that between nanocomposite (Ag-doped ZnO/AgO) and glyphosate was analyzed with FTIR to determine whether nanomaterials could be used as signal enhancers for glyphosates. The results were further supported with the use of atomic force microscopy (AFM) imaging. The glyphosate commercial solutions were intensified 10,000 times when incorporated the ZnO NCs. However, strong chemical interactions between Ag and glyphosate may suppress signaling, making FTIR identification difficult. In short, we have shown for the first time that ZnO NCs are exciting tools with the potential to be used as signal amplifiers of glyphosate, the use of which may be explored in terms of the detection of other molecules based on nanocrystal affinity.