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[This corrects the article DOI: 10.1016/j.clinms.2018.06.002.].
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Background: Aldosterone measurement is critical for diagnosis of primary aldosteronism and disorders of the renin-angiotensin system. We developed an LC-MS/MS method for plasma and urinary aldosterone and compared it to our RIA method. We present a reference interval study for a Belgian population. Methods: 68 plasma and 23 urine samples were assayed for as part of a method comparison. For the reference interval study, we enrolled 282 healthy Caucasian volunteers (114 Male: mean age 35⯱â¯11â¯y and 168 Female: mean age 42⯱â¯13â¯y). A subset of 139 healthy volunteers agreed to a 24-h urine collection. For the method validation, 5 plasma and 8 urine pools were run in triplicate and quadruplicate, respectively, on 3 different days. Results: Between-run imprecision (CV) was 2.8-5.1% for plasma and 4.5-8.6% for urine, except at the low urine concentration of 2.99â¯nmol/L where a CV of 15.4% was observed. The limit of quantitation was 0.04â¯nmol/L for plasma and 6.65â¯nmol/L for urine. Recoveries, based on spiking experiments into natural matrix, did not differ significantly from 100%. Regression comparisons showed that, on average, RIA generated results were 59% and 11% higher than LC-MS/MS for plasma and urine, respectively. The MS reference interval we propose for plasma aldosterone is 0.07â¯nmol/L-0.73â¯nmol/L for women and 0.04â¯nmol/L-0.41â¯nmol/L for men. No gender difference was observed for urine aldosterone. The reference interval was determined to be <60.94â¯nmol/day. Conclusions: The LC-MS/MS method was validated and reference intervals for plasma and urine were established. A significant bias between RIA and LC-MS/MS was noted.