Analgesic potential of transdermal nicotine patch in surgery: a systematic review and meta-analysis of randomised placebo-controlled trials.

OBJECTIVES: We aimed (1) to systematically review the efficacy of transdermal nicotine patches (NP) for postoperative analgesia, (2) to establish the current quality of evidence and assist clinical decision-making on the subject, and (3) to identify methodological limitations and the need for more well-designed studies. MATERIALS AND METHODS: We searched six electronic databases, protocol records, and other sources without date or language restriction until March 2022. To develop the search strategy, we formulated a clinical question by using the PICOD method. Eligibility criteria included randomised placebo-controlled trials on the analgesic potential of NP for surgical procedures. This systematic review followed the PRISMA 2020 statement, and we registered the protocol in PROSPERO (#CRD42020205956). RESULTS: We included 10 randomised placebo-controlled trials (535 patients). The NP administered before induction of anaesthesia and at beginning of surgery reduced the pain immediately after surgery (-0.38; 95% confidence interval [CI]: -0.73 to -0.02), and 6 h (-0.34; 95% CI: -0.68 to -0.01), 12 h (-0.43; 95% CI: -0.71 to -0.15) and 24 h (-0.35; 95%CI: -0.59 to -0.10) after surgery, compared with the placebo patch (PP) group. Sensitivity testing suggests that opioid use could underestimate NP analgesia. Late demand for the first analgesic and consumption of rescue analgesics tended to be lower in the NP group. CONCLUSIONS: The current findings suggest, with low certainty of evidence, the analgesic potential of NP for surgical procedures. CLINICAL RELEVANCE: Perioperative use of NP significantly improved postoperative pain, even when opioids were administered or prescribed. Nevertheless, the clinical relevance should be interpreted with caution, owing to the effect sizes of the summary measures and methodological issues. The analgesic potential of NP as an adjuvant therapy to regulate pain and acute inflammation may offer certain clinical advantages, thus warranting further investigation.

Gasdermin-D activation by SARS-CoV-2 triggers NET and mediate COVID-19 immunopathology.

BACKGROUND: The release of neutrophil extracellular traps (NETs) is associated with inflammation, coagulopathy, and organ damage found in severe cases of COVID-19. However, the molecular mechanisms underlying the release of NETs in COVID-19 remain unclear. OBJECTIVES: We aim to investigate the role of the Gasdermin-D (GSDMD) pathway on NETs release and the development of organ damage during COVID-19. METHODS: We performed a single-cell transcriptome analysis in public data of bronchoalveolar lavage. Then, we enrolled 63 hospitalized patients with moderate and severe COVID-19. We analyze in blood and lung tissue samples the expression of GSDMD, presence of NETs, and signaling pathways upstreaming. Furthermore, we analyzed the treatment with disulfiram in a mouse model of SARS-CoV-2 infection. RESULTS: We found that the SARS-CoV-2 virus directly activates the pore-forming protein GSDMD that triggers NET production and organ damage in COVID-19. Single-cell transcriptome analysis revealed that the expression of GSDMD and inflammasome-related genes were increased in COVID-19 patients. High expression of active GSDMD associated with NETs structures was found in the lung tissue of COVID-19 patients. Furthermore, we showed that activation of GSDMD in neutrophils requires active caspase1/4 and live SARS-CoV-2, which infects neutrophils. In a mouse model of SARS-CoV-2 infection, the treatment with disulfiram inhibited NETs release and reduced organ damage. CONCLUSION: These results demonstrated that GSDMD-dependent NETosis plays a critical role in COVID-19 immunopathology and suggests GSDMD as a novel potential target for improving the COVID-19 therapeutic strategy.

Oxidative Stress in Alzheimer's Disease: Should We Keep Trying Antioxidant Therapies?

The risk of chronic diseases such as Alzheimer's disease is growing as a result of the continuous increasing average life span of the world population, a syndrome characterized by the presence of intraneural neurofibrillary tangles and senile plaques composed mainly by beta-amyloid protein, changes that may cause a number of progressive disorders in the elderly, causing, in its most advanced stage, difficulty in performing normal daily activities, among other manifestations. Therefore, it is important to understand the underlying pathogenic mechanisms of this syndrome. Nevertheless, despite intensive effort to access the physiopathological pathways of the disease, it remains poorly understood. In that context, some hypotheses have arisen, including the recent oxidative stress hypothesis, theory supported by the involvement of oxidative stress in aging, and the vulnerability of neurons to oxidative attack. In the present revision, oxidative changes and redox mechanisms in Alzheimer's disease will be further stressed, as well as the grounds for antioxidant supplementation as adjuvant therapy for the disease will be addressed.

Nutritional supplementation with the mushroom Agaricus sylvaticus reduces oxidative stress in children with HIV.

BACKGROUND: The involvement of free radicals and oxidative stress in HIV infection has been extensively studied, and the benefits of antioxidant supplementation in animal studies have been demonstrated. However, few studies have demonstrated a benefit in clinical studies. OBJECTIVE: To verify the effects of dietary supplementation with Agaricus sylvaticus, a mushroom rich in antioxidants, on the oxidative profile of children born with HIV undergoing antiretroviral therapy. DESIGN: The sample included 24 children (both boys and girls) between two and eight years of age, of whom 10 were HIV positive and received supplementation with Agaricus sylvaticus for a three-month period, and 14 were HIV negative and received no supplementation. At the beginning and conclusion of the study, thiobarbituric acid-reactive substances (TBARS), nitrite and nitrate (NN), Trolox equivalent antioxidant capacity, and the antioxidant capacity of inhibition of diphenyl-picrilhidrazil (DPPH) free radicals were analyzed. RESULTS: Before supplementation, significantly higher values of TBARS and NN, but decreased values of DPPH, were observed in infected subjects when compared with HIV-negative subjects. After supplementation, a reduction of TBARS and NN values and an increase in DPPH and Trolox equivalent antioxidant capacity values were observed in HIV-positive subjects. CONCLUSIONS: The results of the present study suggest the involvement of oxidative stress in HIV infection, with the participation of NN synthesis. Additionally, supplementation reversed oxidative alterations and improved antioxidant defense in infected individuals, and may become a complementary strategy in the treatment of these patients.

HISTORIQUE: L'activité des radicaux libres et du stress oxydatif dans l'infection par le VIH a fait l'objet de nombreuses études, et les bienfaits des suppléments d'antioxydants ont été démontrés dans des études sur des animaux. Cependant, peu d'études en ont confirmé les avantages dans des études cliniques. OBJECTIF: Vérifier les effets de suppléments alimentaires contenant de l'Agaricus sylvaticus, un champignon riche en antioxydants, sur le profil oxydatif des enfants nés avec le VIH sous thérapie antirétrovirale. MÉTHODOLOGIE: L'échantillon se composait de 24 enfants (garçons et filles) de deux à huit ans, dont dix étaient positifs au VIH et ont reçu des suppléments d'Agaricus sylvaticus pendant trois mois, et 14 étaient négatifs au VIH est n'ont pas reçu de suppléments. Au début et à la fin de l'étude, les chercheurs ont analysé les substances réactives à l'acide thio-barbiturique (SRATB), les nitrites et les nitrates (NN), la capacité antioxydante en équivalent Trolox et la capacité antioxydante de l'inhibition des radicaux libres diphényl-picrylhydrazyl (DPPH). RÉSULTATS: Avant la prise de suppléments, les sujets infectés présentaient des valeurs considérablement plus élevées de SRATB et de NN, mais plus faibles de DPPH, que les sujets négatifs au VIH. Après la prise de suppléments, les valeurs de SRATB et de NN diminuaient tandis que celles de DPPH et de la capacité antioxydante en équivalent Trolox augmentaient chez les sujets positifs au VIH. CONCLUSIONS: D'après les résultats de la présente étude, le stress oxydatif est actif dans l'infection par le VIH, avec la participation de la synthèse des NN. De plus, des suppléments renversaient les altérations oxydatives et accroissaient la défense assurée par les antioxydants chez les sujets infectés. De tels suppléments pourraient devenir une stratégie complémentaire au traitement.

Challenges in the pharmacological treatment of patients under suspicion of chronic traumatic encephalopathy: A review.

Chronic traumatic encephalopathy (CTE) is caused by progressive neurodegeneration associated with repetitive head impacts. This disease is more common in professionals who practice contact sports, resulting in a concussion and subconcussive trauma. CTE is characterized by the accumulation of hyperphosphorylated tau protein in neurons, astrocytes, and frontotemporal lobe degeneration. Symptoms are usually nonspecific and overlap with other neurodegenerative diseases, such as Alzheimer's disease and frontotemporal dementia, making it difficult to provide drug treatment for patients with this comorbidity. Therefore, the objective of this article is to present an updated review of the pharmacological treatment of chronic traumatic encephalopathy and its challenges.

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions.

Pathogens can cause a wide variety of infectious diseases. The biological processes induced by the host in response to infection determine the severity of the disease. To study such processes, researchers can use high-throughput sequencing techniques (RNA-seq) that measure the dynamic changes of the host transcriptome at different stages of infection, clinical outcomes, or disease severity.This investigation can lead to a better understanding of the diseases, as well as uncovering potential drug targets and treatments. The protocol presented here describes a complete pipeline to analyze RNA-sequencing data from raw reads to functional analysis. The pipeline is divided into five steps: (1) quality control of the data; (2) mapping and annotation of genes; (3) statistical analysis to identify differentially expressed genes and co-expressed genes; (4) determination of the molecular degree of the perturbation of samples; and (5) functional analysis. Step 1 removes technical artifacts that may impact the quality of downstream analyses. In step 2, genes are mapped and annotated according to standard library protocols. The statistical analysis in step 3 identifies genes that are differentially expressed or co-expressed in infected samples, in comparison with non-infected ones. Sample variability and the presence of potential biological outliers are verified using the molecular degree of perturbation approach in step 4. Finally, the functional analysis in step 5 reveals the pathways associated with the disease phenotype. The presented pipeline aims to support researchers through the RNA-seq data analysis from host-pathogen interaction studies and drive future in vitro or in vivo experiments, that are essential to understand the molecular mechanism of infections.

Involvement of Opioid System and TRPM8/TRPA1 Channels in the Antinociceptive Effect of Spirulina platensis.

Spirulina platensis is a "super-food" and has attracted researchers' attention due to its anti-inflammatory, antioxidant, and analgesic properties. Herein, we investigated the antinociceptive effects of Spirulina in different rodent behavior models of inflammatory pain. Male Swiss mice were treated with Spirulina (3-300 mg/kg, p.o.), indomethacin (10 mg/kg, p.o.), or vehicle (0.9% NaCl 10 mL/kg). Behavioral tests were performed with administration of acetic acid (0.6%, i.p.), formalin 2.7% (formaldehyde 1%, i.pl.), menthol (1.2 µmol/paw, i.pl.), cinnamaldehyde (10 nmol/paw, i.pl.), capsaicin (1.6 µg/paw, i.pl.), glutamate (20 µmol/paw, i.pl.), or naloxone (1 mg/kg, i.p.). The animals were also exposed to the rotarod and open field test to determine possible effects of Spirulina on locomotion and motor coordination. The quantitative phytochemical assays exhibited that Spirulina contains significant concentrations of total phenols and flavonoid contents, as well as it showed a powerful antioxidant effect with the highest scavenging activity. Oral administration of Spirulina completely inhibited the abdominal contortions induced by acetic acid (ED50 = 20.51 mg/kg). Spirulina treatment showed significant inhibition of formalin-induced nociceptive behavior during the inflammatory phase, and the opioid-selective antagonist markedly blocked this effect. Furthermore, our data indicate that the mechanisms underlying Spirulina analgesia appear to be related to its ability to modulate TRMP8 and TRPA1, but not by TRPV1 or glutamatergic system. Spirulina represents an orally active and safe natural analgesic that exhibits great therapeutic potential for managing inflammatory pain disorders.

Gene signatures of autopsy lungs from obese patients with COVID-19.

BACKGROUND & AIMS: Obesity is associated with low grade systemic inflammation and insulin resistance. Although metabolic and immunological changes may contribute to the increased risk for COVID-19 mortality in obese, little is known about the impact of obesity in the lungs of patients with COVID-19. METHODS: We analyzed gene expression profiles of autopsy lungs of a cohort of 14 COVID-19 patients and 4 control individuals. Patients were divided into 3 groups according to their comorbidities: hypertension, type 2 diabetes (T2D) and obesity. We then identified the molecular alterations associated with these comorbidities in COVID-19 patients. RESULTS: Patients with only hypertension showed higher levels of inflammatory genes and B-cell related genes when compared to those with T2D and obesity. However, the levels of IFN-gamma, IL22, and CD274 (a ligand that binds to receptor PD1) were higher in COVID-19 patients with T2D and obesity. Several metabolic- and immune-associated genes such as G6PD, LCK and IL10 were significantly induced in the lungs of the obese group. CONCLUSION: Our findings suggest that SARS-CoV-2 infection in the lungs may exacerbate the immune response and chronic condition in obese COVID-19 patients.

Proximity of the maxillary artery to the neck of the mandibular condyle: anatomical study.

PURPOSE: The objective of this study was to evaluate the anatomical distance of the maxillary artery, the most superior portion of the condyle and subcondyle in standardized coronal sections, acquired from 16 hemifaces in 8 cadavers. METHODS: Measurements were taken to evaluate the distance between the uppermost portion of the mandibular condyle and the height of the maxillary artery (AB) and between the lateral (CD) and medial (EF) sides of the condylar neck to the artery. RESULTS: The mean for AB was 21.1 mm hemiface right and 22.9 mm left on the anterior face, 22.5 mm and 20.7 mm, respectively, on the rear face. The CD measurement presented 6.7 mm right side in the anterior face and 6.3 mm left, and the posterior face was 6.3 mm right side and 5.4 mm left; EF presented a mean of 2.2 mm right and 1.5 mm left on the anterior face and 1.6 mm for both sides on the posterior face. CONCLUSIONS: There was no statistically significant difference between hemifaces. The proximity of the maxillary artery to the medial face of the neck of the mandibular condyle is millimetric and presents risks of severe hemorrhage for interventions in the infratemporal fossa.

Percepção dos profissionais da saúde no enfrentamento da covid-19: principais desafios no processo de trabalho / Healthcare providers' perception of COVID-19: main challenges in the work process / Percepción de los profesionales de la salud sobre el afrontamiento de la COVID-19: principales retos en el proceso de trabajo

O cenário advindo como consequência da pandemia de covid-19 acarretou diversas mudanças na vida dos profissionais de saúde. A percepção desse grupo torna-se indispensável para a compreensão do contexto, visto que esses trabalhadores são figuras essenciais no enfrentamento da doença. O objetivo deste estudo foi analisar a percepção de gestores e profissionais que atuaram durante a primeira onda da pandemia de covid-19 no município de Sobral (CE). Trata-se de uma pesquisa exploratório-descritiva, com abordagem qualitativa, feita por meio da aplicação de um instrumento de coleta de dados. O estudo foi realizado com gestores e trabalhadores que atuaram de março a dezembro de 2020 no município. A coleta foi feita no formato híbrido, com auxílio da plataforma Google Meet, fundamentada na metodologia snowball sampling e no método de saturação. Após a coleta, os discursos foram transcritos ­ utilizando a ferramenta Microsoft Word ­ e analisados ­ com suporte do software NVivo11. Foram contactados 68 profissionais. Identificou-se predominância final de enfermeiros, sexo feminino e atuantes na atenção terciária. Após análise e categorização, identificaram-se 12 unidades de registro. Foram dispostos os principais desafios e dificuldades acarretados para os processos de trabalho e suas subcategorias: a sobrecarga de trabalho e o desgaste físico; a reorganização dos serviços em resposta à nova realidade; o desconhecimento sobre a doença e seu impacto psicológico no fazer profissional; e o fator social e a dificuldade de seguimento da assistência na atenção primária. Por fim, conclui-se que os desafios identificados se inter-relacionam, repercutindo uns nos outros, e constata-se a importância de conhecer esses entraves para ações mais bem direcionadas e estratégicas.

The scenario that has arisen in response to COVID-19 has led to a variety of changes in the lives of healthcare providers. The perception of this group is essential to understanding this context since they are key figures in coping with the disease. This study aimed to analyze the perception of managers and professionals who worked during the first wave of the COVID-19 pandemic in the municipality of Sobral/CE. This is an exploratory-descriptive study with a qualitative approach that applied a collection instrument with managers and workers who worked from March to December 2020 in the municipality. Data were collected using the Snowball Smapling and the saturation method in a hybrid format using the Google Meet platform. After the collection, responses were transcribed using Microsoft Word and analyzed with the aid of NVivo11. In total, 68 professionals were contacted, with a final predominance of female nurses working in tertiary care. After analysis and categorization, 12 recording units were identified. The main challenges and difficulties for work processes and their subcategories include work overload and physical exhaustion; reorganization of services in response to the new reality; lack of knowledge about the disease and its psychological impact on professional practice; and the social factor and the difficulty of following up care in primary care. Finally, we conclude that the identified challenges are interrelated and have repercussions on each other, and that it is important to understand these obstacles to take more targeted and strategic actions.

El escenario que ha surgido en respuesta a la pandemia de la COVID-19 ha provocado diversos cambios en la vida de los profesionales de la salud. La percepción de este grupo es indispensable para comprender el contexto, ya que estos trabajadores son figuras esenciales en el enfrentamiento a la enfermedad. El objetivo de este estudio fue analizar la percepción de gestores y profesionales que actuaron durante la primera ola de la pandemia de la COVID-19 en el municipio de Sobral (Ceará, Brasil). Se trata de un estudio exploratorio-descriptivo, con enfoque cualitativo, realizado mediante la aplicación de una herramienta de recolección de datos. En esta investigación participaron gestores y trabajadores que actuaron de marzo a diciembre de 2020 en el municipio. Se realizó la recolección en formato híbrido, con ayuda de la plataforma Google Meet, basada en el método bola de nieve y en el método de saturación. Después, se transcribieron los discursos, utilizando la herramienta Microsoft Word, y se analizaron con el apoyo del software NVivo11. Se contactaron 68 profesionales. Se identificó predominio de enfermeros, mujeres y trabajadores de atención terciaria. Después del análisis y de la categorización, se identificaron 12 unidades de registro. Se presentaron los principales retos y dificultades que generan los procesos de trabajo y sus subcategorías: la sobrecarga de trabajo y el agotamiento físico; la reorganización de los servicios en respuesta a la nueva realidad; el desconocimiento de la enfermedad y su impacto psicológico en la práctica profesional; y el factor social y la dificultad de seguimiento de los cuidados en atención primaria. Finalmente, se concluye que los retos identificados están interrelacionados, impactándose entre sí, y se señala la importancia de conocer estos obstáculos para emprender acciones más específicas y estratégicas.

Conjunctivitis epidemiological profile in an ophthalmological service in the metropolitan region of Fortaleza - Ceará ­ Brazil / Perfil epidemiológico da conjuntivite em um serviço oftalmológico na região metropolitana de Fortaleza - Ceará ­ Brasil

Objective: to evaluate the seasonal and endemic characteristics of conjunctivitis at the ophthalmology service of the Leiria de Andrade Foundation (FLA) in the last ten years to trace the epidemiological profile of conjunctivitis in Fortaleza - CE. Methods: this was a descriptive and epidemiological study based on quantitative and qualitative analysis, retrospectively, from January to December 2012 to 2019, with a projection for the years 2020 and 2021. Results: 107,778 medical records were analysed, with endemic and seasonal fluctuation, being the months of October to December more frequent in the intervals of the highest incidence of the disease. Two peaks were notorious, one with epidemic characteristics, from July 2013 to November 2014, and the other with outbreak characteristics, probably due to a national-level epidemic, between January and April 2018. The most affected age group was between 19 and 59 years, covering about 72% of cases, with no statistical difference between genders. Conclusion: according to the study data, it was possible to infer that the epidemiological scenario of Fortaleza - CE is in line with the literature regarding age range and sex. The endemicity of the disease reinforces its relevance in the scenario of the Unified Health System (SUS) of the region.

Objetivo: avaliar as características sazonais e endêmicas da conjuntivite no serviço de oftalmologia da Fundação Leiria de Andrade (FLA) nos últimos 10 anos, a fim de traçar o perfil epidemiológico da conjuntivite em Fortaleza - CE. Métodos: estudo descritivo e epidemiológico, com base em análise quantitativa e qualitativa, retrospectivamente, de janeiro a dezembro de 2012 a 2019, com projeção para os anos de 2020 e 2021. Resultados: foram analisados 107.778 prontuários, com variação endêmica e sazonal, estando os meses de outubro a dezembro com maior frequência dentro dos intervalos de maior incidência da doença. Notaram-se dois picos, um com características epidêmicas, de julho de 2013 a novembro de 2014, e outro com características de surto, provavelmente decorrente de uma epidemia de nível nacional entre janeiro e abril de 2018. A faixa etária mais afetada foi entre 19 e 59 anos, compreendendo cerca de 72% dos casos, sem diferença estatística entre os gêneros. Conclusão: de acordo com os dados do estudo, foi possível inferir que o cenário epidemiológico de Fortaleza - CE está de acordo com a literatura quanto à faixa etária e ao sexo. A endemicidade da doença reforça sua pesquisa no cenário do Sistema Único de Saúde (SUS) da região

Drogas antiagregantes plaquetárias e anticoagulantes em pacientes que se submeterão à cirurgia oral: conduta / Antiagregant platelet and anticoagulant drugs in patients who will submit oral surgery: conduct

Objetivo: Como a frequência de pacientes em uso de anticoagulantes e antiagragantes plaquetários nos consultórios odontológicos é crescente, este trabalho objetivou avaliar através de Revisão de Literatura, qual o melhor manejo desses medicamentos na prática odontológica perioperatória. Metodologia: Trata-se de uma revisão integrativa da literatura, utilizadas as bases de dados Scielo e PubMed. Foram escolhidos os seguintes descritores disponíveis na BVs e PubMed em inglês "Platelet Aggregation Inhibitors", "Oral Surgical Procedures" e "Antigoagulants" no período de 2016 a 2021. Também foram consultados livros e sites de diretrizes do Governo. Foram escolhidos 20 artigos para elaboração da pesquisa. Resultados: doenças cardiovasculares e outras condições clínicas pró-coagulantes tem prevalência crescente e são conhecidos fatores de risco para a ocorrência de fenômenos tromboembólicos graves. A terapia antitrombótica tem papel definido nesses casos. No perioperatorio de cirurgias orais, a decisão por suspender ou manter a terapia deve ser individualizada e pode ser orientada por guidelines. Conclusão: procedimentos orais de baixo risco de sangramento podem ser conduzidos sem a descontinuação da terapia antitrombótica. Cirurgias de moderado a alto risco frequentemente requerem suspensão temporária das medicações para fins de minimizar os riscos de complicações hemorrágicas... (AU)

Objective: As the frequency of patients using anticoagulants and antiplatelet agents in dental offices is increasing, this study aimed to evaluate, through a Literature Review, which is the best management of these medications in dental perioperative practice. Methodology: This is an integrative literature review, being used Scielo and PubMed databases. The following descriptors available in BVs and PubMed "Platelet aggregation inhibitors", "Oral Surgical Procedures" and "Antigoagulants" were used, from 2016 to 2021. In addition, the search was also performed in guideline books and Government websites. Twenty articles were chosen for research elaboration. Results: established cardiovascular disease and other procoagulant clinical conditions have an increasing prevalence, especially among the elderly, and are known risk factors for the occurrence of severe thromboembolic phenomena. Antithrombotic therapy has defined role in these cases. In the perioperative period of oral surgery, the decision to suspend or maintain therapy must be individualized and may be guided by guidelines. Age appears as a clinical criterion in the main ones used. Conclusion: oral procedures with low risk of bleeding can be carried out without discontinuing antithrombotic therapy. Moderate to high-risk surgeries usually require its temporary suspension in order to minimize the risk of bleeding complications... (AU)

Participação popular em um município do interior do Ceará: análise a partir da percepção de enfermeiros e gerentes da Atenção Primária à Saúde / Popular participation in a city in the countryside of Ceará: Analysis from the perception of nurses and managers of Primary Health Care

Este estudo analisa a percepção de enfermeiros e gerentes da APS acerca da percepção de enfermeiros e gerentes da Atenção Primária à Saúde (APS) no que se refere aos mecanismos da participação popular. Trata-se de um estudo descritivo-exploratório de abordagem qualitativa, cujos participantes foram enfermeiros e gerentes da Estratégia Saúde da Família de um município no interior do Estado do Ceará. A coleta de dados foi realizada por meio de entrevista semiestruturada e teve a análise feita por meio da técnica de Análise de Conteúdo de Bardin com suporte do software N VIVO 11. O estudo respeitou os princípios éticos referentes à Resolução 466/2012 do CNS. O estudo revela a adoção de diferentes estratégias pelos profissionais para fomentar o protagonismo dos usuários no controle social. Entretanto, apesar disso, ainda pode-se perceber a resistência dos indivíduos a exercerem seus direitos de cidadania. Assim, cabe aos profissionais incentivar o comportamento participativo dos usuários.

This study analyzes the perception of nurses and PHC managers about the articulation of the health service with the community. This is a descriptive-exploratory study with a qualitative approach, whose participants were nurses and managers of the Family Health Strategy of a municipality in the countryside of the State of Ceará. Data collection was carried out through semi-structured interviews and the analysis was carried out using the Bardin Content Analysis technique, supported by the N VIVO 11 software. The study respected the ethical principles related to CNS Resolution 466/2012 . The study reveals the adoption of different strategies by professionals to foster the role of users in social control. However, despite this, it is still possible to perceive the resistance of individuals to exercise their citizenship rights. Thus, it is up to professionals to strengthen the politicized bases of users.

Dental trauma with intrusive luxation and six-year monitoring: clinical case report / Traumatismo dentário com luxação intrusiva com acompanhamento de seis anos: relato de caso clínico

Objective: this study is designed to report a clinical intrusion case, which exhibited enamel hypoplasia on the crown of tooth 11 and which underwent clinical and X-ray monitoring for six years. Case report: A threeyear- old female patient exhibited intrusion of deciduous teeth 51, 52, 61, and 62. After X-ray, it was detected that teeth 51 and 61 had perforated the nasal cavity floor, but all four teeth had re-erupted and began to be monitored. After six months, teeth 51 and 61 exhibited bone loss in the interproximal area, as well as mobility, which led to tooth extraction. Teeth 52 and 62 re-erupted in a satisfactory position, and exhibited no signs and symptoms of infection, periapical alteration, or mobility. These teeth were therefore maintained in the arch. After six years, it was detected that tooth 11 had erupted with enamel hypoplasia. Final considerations: Longterm clinical and X-ray control proved essential, as it allowed for the early diagnosis of potential alterations, and minimized potential sequelae to the permanent teeth.(AU)

Objetivo: relatar um caso clínico de intrusão com acompanhamento clínico e radiográfico de 6 anos, que apresentou hipoplasia de esmalte na coroa do dente 11. Relato de caso: paciente de 3 anos, sexo feminino, apresentou intrusão dos dentes decíduos 51, 52, 61 e 62. Após radiografia, foi detectado que os dentes 51 e 61 tinham perfurado o soalho da fossa nasal, porém, ocorreu re-erupção dos 4 dentes e foi realizado acompanhamento. Após 6 meses, nos dentes 51 e 61, foram observadas perda óssea na região interproximal e mobilidade, optando-se pelas exodontias. Os dentes 52 e 62 re-erupcionaram em posição satisfatória e com ausência de sinais e sintomas de infecções, alterações periapicais e mobilidade, sendo mantidos no arco. Após 6 anos, foi observado que o dente 11 erupcionou apresentando hipoplasia de esmalte. Considerações finais: mostrou-se essencial o controle, clínico e radiográfico, em longo prazo, diagnosticando precocemente possíveis alterações e minimizando sequelas que podem acometer os dentes permanentes.(AU)

Traqueostomia: condutas e técnica / Tracheostomy: Conducts and Technique

A traqueostomia é um procedimento cirúrgico que objetiva permitir desobstrução das vias aéreas superiores do paciente com intuito de garantir a vida. O procedimento é realizado em situações de comprometimento do trato respiratório superior por tumores, cirurgias, traumas faciais graves ou infecções. A traqueostomia também é realizada para prevenir lesões laríngeas ou nas vias aéreas superiores causadas por intubação traqueal prolongada. O objetivo desse trabalho é apresentar técnicas e condutas corretas... (AU)

Tracheostomy is a surgical procedure that aims to allow unblocking of the patient's upper airways in order to ensure life. The procedure is performed in situations of involvement of the upper respiratory tract by tumors, surgery, severe facial trauma or infections. Tracheostomy is also performed to prevent laryngeal or upper airway injuries caused by prolonged tracheal intubation. The objective of this work is to present a techniques and correct conduct... (AU)

[Alterations in cholesterol, triglyceride and total phospholipid levels in plasma of Callithrix jacchus (sagüi) reinfected by Schistosoma mansoni]. / Alterações nos níveis de colesterol, triglicerídeo e fosfolipídeo total em plasma de Callithrix jacchus (sagüi) reinfectado por Schistosoma mansoni.

Little information is available on the lipid changes caused by Schistosoma mansoni reinfection. In this work it was evaluated alteration in the plasma lipids due to one reinfection by Schistosoma mansoni in the non human primate Callithrix jacchus (sagüi). Blood samples from C. jacchus, prior and after 60 days infection and reinfection, were collected by intravenous puncture, anticoagulated with EDTA (1mg/mL) and centrifuged at 2,500 xg, in order to obtain the plasma. Total cholesterol, cholesteryl ester, total phospholipid and triglyceride levels were determined by spectrophotometer methods. The results showed that there are significant reduction in cholesterol total, cholesteryl ester, total phospholipid and triglyceride concentrations in plasma of animals reinfected by Schistosoma mansoni, in comparison to the same animals prior and after one infection. This study showed that a second infection of Callithrix jacchus by Schistosoma mansoni causes plasma lipid alterations, which are more significant than after a single infection.

Saúde bucal em crianças com câncer: conhecimentos e práticas dos cuidadores / Oral health in children with cancer: knowledge and practices of caregivers

Objetivo: avaliar os conhecimentos e as práticas dos cuidadores sobre saúde bucal em crianças com câncer, na instituição beneficente Lar Amigos de Jesus, em Fortaleza, Ceará, além de identificar as principais complicações bucais decorrentes do tratamento oncológico e como é estabelecida a relação médico-dentista desses pacientes. Materiais e método: a população do estudo foi composta por 15 cuidadores, que são pessoas responsáveis pelo acompanhamento das crianças acolhidas pela entidade. A coleta de dados foi realizada por meio de entrevista semiestruturada. Para análise dos dados, foi utilizada a técnica de análise de conteúdo temático. Resultados: todos os entrevistados eram do sexo feminino e não trabalhavam. Quanto ao grau de parentesco com a criança, 14 das entrevistadas eram as mães e uma entrevistada era a avó. Após a decomposição do corpus e da codificação das unidades temáticas, o material coletado foi estruturado nas seguintes categorias: cuidados em saúde bucal, complicações bucais decorrentes do tratamento oncológico, relação médico-dentista, saúde geral e saúde bucal. Conclusão: a manutenção da higiene bucal das crianças, antes, durante e após o tratamento antineoplásico, é da maior relevância para evitar o aparecimento de complicações bucais decorrentes das diferentes terapias aplicadas no combate ao câncer. Entretanto, pode-se perceber a falta de informação sobre as práticas de higiene bucal e a dificuldade em entender a importância da saúde bucal para a manutenção da saúde sistêmica. Ficou evidenciada a importância da inserção do cirurgião-dentista na equipe multidisciplinar para acompanhamento do paciente oncológico pediátrico, visando à melhoria da qualidade de vida desse sujeito. (AU)

Objective: to assess the knowledge and practices of caregivers on oral health in children with cancer at Lar Amigos de Jesus - a charity institution in Fortaleza, Ceará, Brazil. It also aims to identify the main oral complications from cancer treatment and the establishment of the physician-dentist relationship of these patients. Materials e method: the study population consisted of 15 caregivers responsible for accompanying the children welcomed by the institution. The data was collected using semi-structured interviews. The data was analyzed with the thematic content analysis technique. Results: all respondents were women and did not work. As for the relationship to the children, 14 of the respondents were their mothers and one was the child's grandmother. After decomposing the corpus and coding the thematic units, the material collected was structured into the following categories: oral health care, oral complications from cancer treatment, physician-dentist relationship, general health, and oral health. Conclusion: maintaining the oral hygiene of children before, during, and after the antineoplastic treatment is essential to prevent the appearance of oral complications from the different therapies used to fight cancer. However, there is a lack of information on oral hygiene practices and difficulty understanding the importance of oral health to the maintenance of systemic health. The insertion of a dentist in the multidisciplinary team to follow-up pediatric cancer patients is evidenced, aiming to improve the children's quality of life. (AU)

Alternative of Lower-Cost Glass-Ionomer Sealant in the Prevention of Caries Lesions in Brazilian Children

Objective: To compare retention, preventive effectiveness and longevity of two different highviscosity glass-ionomer sealants Ketac Molar® and Maxxion R® in the sealing of erupting and out-of-occlusion first permanent molar in children aged 5-7 years. Material and Methods: Children with past caries experience with healthy erupting first permanent molar were included in the study. Teeth 16 and 46 were sealed with Ketac Molar® and teeth 26 and 36 were sealed with Maxxion R®. After 8 months, the retention of sealants was evaluated using the following criteria: total retention; presence of sealant in two thirds of the occlusal surface; presence of sealant in one third of the surface; and total absence of the sealant. Results: Statistical difference between materials was observed, and Ketac Molar® retention was superior to that of Maxxion R® (p<0.05). Regarding the development of caries in sealed first permanent molar, none of the teeth sealed with Ketac Molar® or Maxxion R® developed caries lesion. Conclusion: Ketac Molar® retention is significantly superior to that of Maxxion R®, but the preventive effectiveness in the development of carious lesions is similar for both, which is extremely interesting for the field of public health, since the cost of the material is significantly lower.

Microleakage analysis of dental caries lesions sealed with flow resin and compared to microhybrid resin restorations in dentin / Avaliação da microinfiltração de lesões de cárie dentinárias seladas com resina flow e restauradas com resina microhíbrida

ABSTRACT Objective: To evaluate the sealing of cavities of dentinal occlusal caries lesions, reproduced in vitro, with flow resin compared to cavity restorations presenting healthy dentin using microhybrid composite resin. Methods: The sample consisted of 27 healthy deciduous molars where cavities of approximately 2 mm in the fossa region were performed and occlusal cleft of each tooth were sealed, impermeabilization was performed and the sample was randomly divided into 2 groups: group I underwent cariogenic challenge and occlusal sealing with resin flow. The teeth of group II were restored with microhybrid composite resin. The teeth were immersed in 5% methylene blue for 8 hours at 37° C and washed until all the dye was removed from the surface. The teeth were sectioned in the mesio-distal direction. The penetration of the dye was evaluated: 0- no penetration; 1- dye penetration up to 1/3 of the restoration; 2- dye penetration up to 2/3 of the restoration depth; 3 - penetration of dye into the pulp wall. The results were analyzed by the Biostat 4.0 program. Descriptive analysis and the mode among the examiners submitted to the Mann-Whitney test. Results: There was no significant difference in microleakage between restoration performed in healthy dentin with microhybrid composite resin or maintenance of infected dentin in primary teeth sealed with resin flow (p = 0.6035). Conclusion: It was concluded that the marginal infiltration of primary molars sealed with microhybrid composite resin and resin flow was not influenced by the removal -or not -of the carious tissue or the material used.

RESUMO Objetivo: Avaliar o selamento de cavidades de lesões de cárie oclusais em dentina, reproduzidas in vitro, com resina flow comparando com restaurações em cavidades com dentina hígida utilizando resina composta microhíbrida. Métodos: Foram selecionados 27 molares decíduos hígidos, realizadas cavidades de aproximadamente 2 mm na região de fossa e fissura da face oclusal de cada dente, impermeabilização e divisão em 2 grupos aleatoriamente: grupo I foram submetidos ao desafio cariogênico e selamento oclusal com resina flow. Os dentes do grupo II foram restaurados com resina composta microhíbrida. Os dentes foram imersos em azul de metileno a 5% durante 8 horas a 37 ° C e lavados até que todo o corante fosse removido da superfície. Os dentes foram seccionados no sentido mesio-distal. A penetração do corante foi avaliada: 0- nenhuma penetração; 1- a penetração de corante até 1/3 da restauração; 2- a penetração de corante até 2/3 da restauração profundidade; 3- penetração de corante para a parede pulpar. Os resultados foram analisados pelo programa Biostat 4.0. Foi feita análise descritiva e a moda entre os examinadores submetidas ao teste de Mann-Whitney. Resultados: Não houve diferença significante na microinfiltração entre restauração realizada em dentina hígida com resina composta microhíbrida ou a manutenção da dentina infectada em dentes decíduos selados com resina flow (p=0.6035). Conclusão: A remoção ou não do tecido cariado, assim como o material utilizado, não influenciou na infiltração marginal de molares decíduos selados com resina composta microhíbrida e resina flow.

Selamento de lesões de cárie oclusais em molares decíduos: relato de dois casos clínicos

Objetivo: foi relatar dois casos clínicos sobre selamentode lesões de cárie em dentina com resina flow em molaresdecíduos. Relato de caso: foram selecionadas duascrianças, de 4 e 6 anos de idade, que apresentavam lesãode cárie oclusal, com abertura menor do que trêsmilímetros e profundidade envolvendo a dentina: umalocalizava-se na metade externa, e outra, iniciando aprogressão para metade interna. O selamento das lesõesfoi realizado com resina flow (Natural Flow - DFL),depois de prévio condicionamento com ácido fosfóricoa 37% e aplicação do sistema adesivo na cavidade. Foirealizado acompanhamento clínico e radiográfico deseis meses, analisando a integridade do material de selamentoe a progressão das lesões de cárie. Nos dois casos,houve retenção completa do material selador, quese manteve íntegro. Radiograficamente, as lesões de cárienão apresentaram progressão, pelo contrário, houvesinais de regressão, aumentando as chances de um bomprognóstico. Considerações finais: o selamento de lesõesde cárie com resina flow, desde que devidamentecontrolado clínica e radiograficamente, pode propiciara paralisação de lesões de cárie em dentina, sendo umaabordagem altamente vantajosa, não invasiva, de baixocusto e que preserva os tecidos dentais.