Human temporomandibular joint and myofascial pain biochemical profiles: a case-control study.

Neurobiological mechanisms of human musculoskeletal pain are poorly understood. This case-control study tested the hypothesis that biomarkers within temporomandibular muscle and joint disorders (TMJD) subjects' masseter muscles or temporomandibular joint (TMJ) synovial fluid correlate with plasma biomarker concentrations. Fifty subjects were recruited and categorized into TMJD cases (n=23) and pain-free controls (n=27) at the University of Minnesota School of Dentistry. Prior to specimen collection, pain intensity and pressure pain threshold masseter muscles and the TMJs were assessed. We collected venous blood; biopsied masseter muscle; and sampled TMJ synovial fluid on the subjects' side of maximum pain intensity. We assayed these tissues for the presence of nerve growth factor (NGF), bradykinin (BK), leukotreine B(4) (LTB(4) ) and prostaglandin E(2) (PGE(2) ), F(2) -isoprostane (F(2) I) and substance P (SP). The data was analyzed using Spearman Correlation Coefficients. We found that only plasma concentrations of bradykinin statistically correlated with synovial fluid concentrations (ρ=-0·48, P=0·005), but no association was found between pain intensities. The data suggests that biomarkers used to assess TMJD need to be acquired in a site-specific manner. We also discovered that F(2) I concentrations were associated with muscle pain intensity and muscle pressure pain threshold (PTT) (ß=0·4, 95%CI: 0·03-0·8) and joint PPT (ß=0·4, 95%CI: 0·07-0·8) suggesting that muscle oxidative stress is involved in myofascial pain and that F(2) -I may be a biomarker for myofascial pain.

Evidence of oxidative stress in temporomandibular disorders: a pilot study.

Oxidative stress is involved in the pathogenesis of many conditions and is caused by free radicals in concentrations that overwhelm the natural scavenging mechanisms and cause pain and inflammation. This investigation sought to determine whether pain from temporomandibular disorders was associated with increased oxidative stress as measured by biomarkers in saliva and serum. Both salivary and serum levels of the oxidative stress biomarkers including 8-hydroxydeoxyguanosine, malondialdehyde and total antioxidant status were compared in patients with mild and severe TMJD pain and with healthy controls. These biomarkers were determined spectrophotometrically in saliva and serum from 10 high TMJD pain patients, 10 low TMJD pain patients, and 10 healthy control subjects from National Institute of Dental Research's TMJ Implant Registry and Repository. Linear and logistic regression analyses were used to evaluate the association between each biomarker and TMJD pain. The mean levels of log 8-hydroxydeoxyguanosine (saliva P < 0·0001, serum P = 0·0008), malondialdehyde (saliva P = 0·002, serum P = 0·004) and total antioxidant status (saliva P = 0·005; serum P = 0·001) achieved statistically significant differences between groups. In linear regression analysis, both salivary and serum levels of each biomarker were associated with TMJD pain. In a multivariable analysis, again, both salivary levels and serum levels were also different between groups. Salivary levels of oxidative stress ratios of 8-hydroxydeoxyguanosine, malondialdehyde and total antioxidant status were significantly different between patients with TMJD pain and controls and was comparable to that in serum. These biomarkers hold promise as a potential diagnostic and therapeutic strategy.

Clinical decision on pit and fissure sealing according to the occlusal morphology. A descriptive study.

BACKGROUND: The objective of this descriptive study was to evaluate the clinical decision on sealing pits and fissures according to the occlusal morphology in patients with low individual caries risk (ICR). MATERIALS AND METHODS: A total of 222 dentists, 86 affiliated to the French Society of Paediatric Odontology (SFOP) and 136 general practice dentists (GPs), answered the same questionnaire with illustrations of 4 occlusal surfaces of permanent molars: they indicated firstly if these were at risk and secondly the corresponding decision regarding sealing. This questionnaire assessed the decision on widening pits and fissures before sealing and the type of sealant material used. Multivariate logistic regression analyses were performed to identify the factors associated with the clinical decision to widen pits and fissures. RESULTS: Sealing of at-risk teeth was indicated by 89% of dentists, whereas sealing of not at-risk occlusal surfaces was recommended by 46%. SFOP dentists were more prone to recommend pit and fissures sealants. The multivariate analyses demonstrated that only the type of material was associated with the clinical decision to widen pits and fissures. Forty eight percent of dentists choose the same material in all clinical situations. CONCLUSION: The wide variations in sealant use and placement technique implies there is no apparent consensus among GP and SFOP dentists. Although the criteria are similar in numerous scientific societies, not all dentists are acting upon these recommendations.

Deep carious lesions management in children and adolescents: a questionnaire survey among dentists practicing paediatric dentistry in France.

INTRODUCTION: While many questionnaire surveys have been undertaken worldwide to investigate practices toward deep carious lesion (DCL) management in adults, very few are related to children and adolescents. The present cross-sectional study aimed to assess DCL management in children and adolescents among dentists practicing paediatric dentistry in France (Fr-DPPDs). The secondary objective was to compare practices between Fr-DPPDs and dentists registered in the European Academy of Paediatric Dentistry (EAPD). METHODS: A questionnaire was electronically administrated to members of the CEOP (Collège des Enseignants en Odontologie Pédiatrique), the SFOP (Société Française d'Odontologie Pédiatrique), and the EAPD. Descriptive analyses, Chi-square and McNemar tests, ANOVA, crude and adjusted binary logistic regression analyses were performed. RESULTS: A total of 99 Fr-DPPDs and 146 EAPD members answered the questionnaire. Among the Fr-DPPDs, the preferred caries removal (CR) methods were the complete CR in one step for primary teeth and mature permanent teeth (respectively, 70% and 48%) and in two steps for immature permanent teeth (39%). EAPD members were more likely, than Fr-DPPDs, to choose selective CR versus complete CR in primary teeth (odds ratio = 2.60; 95% CI 1.39-4.85). Moreover, for primary or immature permanent teeth, general practitioners were less likely to choose selective CR than specialists and exclusive practitioners in paediatric dentistry, (p < 0.001). CONCLUSION: Tooth type [primary, permanent (immature or mature)] seemed to influence DCL management. Fr-DPPS should prioritise pulpal vitality when managing DCL.

Manual toothbrush wear and consequences on plaque removal.

OBJECTIVE: The aim of this study was to determine the mean time interval before needing to discard different types of manual toothbrushes based on wear, and on the impact of progressive wear on plaque removal efficacy. METHODOLOGY: Two cross-over randomized clinical trials involving the same 12 volunteers tested four toothbrushes of different designs. In Study 1, the brushes were the Butler 211 (BTB) and Fluocaril Sensia (FSTB). In Study 2, the brushes were Elmex InterX (ETB) and Elgydium Interactive (ELTB). In both trials, the volunteers randomly used one of the two toothbrushes for two minutes twice a day during the first week, and the other brush during the following week, and continued this alternating routine for two months. An image acquisition system and the Visilog 5.2 image analysis program were used to calculate the wear index (WI) after one and two months of use for each toothbrush. The OHI-S plaque index (PI) was recorded after one and three minutes of tooth brushing at one and two months. The Friedman or Kruskal-Wallis test was used to compare data. RESULTS: Except for the ELTB, the wear of the toothbrushes was greater during the first month of brushing than during the second. The total WI was FSTB 35.46 (range = 62.11), ELTB 30.14 (range = 67.92), ETB 48.77 (range = 123.87), and BTB 98.20 (range = 134.75). The differences were significant at p = 0.008. The post one-minute PI scores significantly increased with wear, except in the case of ELTB (p = 0.244). When comparing all toothbrushes' PI at the one and three-minute tooth brushing times, there were significant differences at one month among them at p < 0.0001. CONCLUSION: The amount of wear depended on the toothbrush design. It was greater for rectangular tooth brushing areas or when bristles had smaller diameters and different heights. With two-month-old toothbrushes, plaque removal was better when there was less measurable wear.

Performance of a recent light fluorescence device for detection of occlusal carious lesions in children and adolescents.

AIM: To assess the validity and reliability of a recent light fluorescence device, Soprolife® (Sopro-Acteon group) in detecting occlusal caries in children and adolescents and to compare its diagnostic performance with DIAGNOPen® (Kavo). METHODS: A multi-centre study was carried out to validate Soprolife® in 103 children, aged from 5-15 years, on 310 primary and 433 permanent posterior teeth. The sensitivity (SE), specificity (SP) and the area under the Receiver Operating Characteristic (ROC) curve (AUC) were evaluated using visual International Caries Detection and Assessment System (ICDAS) and radiographic examinations as the gold standards. The performance of the Soprolife® was compared with that of the DIAGNOPen® on the same teeth. The reproducibility was assessed using weighted Kappa coefficient. RESULTS: When all carious lesions using ICDAS 1-6 were considered, SE, SP and AUC for the Soprolife® were 88.50, 70.73 and 0.84 respectively. The validity was significantly higher for primary teeth (AUC = 0.90) than for permanent teeth (0.80); the validity of the Soprolife® (0.84) was significantly higher than that of DIAGNOPen® (0.80). The inter- and intra-examiner kappa coefficients were 0.87 and 0.85 respectively. CONCLUSION: The Soprolife® was a valid instrument providing reproducible results, particularly for primary teeth.

Altered Levels of Salivary 8-oxo-7-hydrodeoxyguanosine in Breast Cancer.

Oxidative stress has been implicated in the pathogenesis of breast cancer (BC). To determine whether BC is associated with altered salivary redox homeostasis, we performed a case-control study assessing the relationship between BC and 8-oxo-7-hydrodeoxyguanosine (8-oxodG), a marker for oxidative damage to DNA. Enzyme-linked immunosorbent assay for 8-oxodG was used on whole, unstimulated saliva of 134 BC patients and 226 healthy controls. Associations of the redox data were assessed by analysis of variance and logistic regression analysis. Our results revealed that there were 1) significantly lower mean levels of salivary 8-oxodG in BC patients versus controls ( P = 0.0005), 2) significantly lower levels among participants who did not receive radiotherapy and/or chemotherapy as compared with controls ( P < 0.0001), 3) significantly lower levels among BC patients who did not receive these treatments than among those who did ( P < 0.02), 4) and no significant differences in mean 8-oxodG levels among BC patients positive or negative for estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 ( P ≥ 0.08). Our results suggest that BC is associated with decreased levels of oxidatively modified DNA in saliva. Knowledge Transfer Statement: The results of our current case-control study indicate that the molecular biomarker of oxidative stress 8-oxo-7-hydrodeoxyguanosine, measured from saliva, is associated with breast cancer. Our findings may provide the basis for future studies on molecular biomarkers of oxidative stress and breast cancer using saliva as an accessible and noninvasive tissue.

Relationship between dental factors and risk of upper aerodigestive tract cancer.

We examined the relationship between dental health variables and risk of upper aerodigestive tract (UADT) cancers in a case-control study in Southern Brazil. The study population included 717 cases of cancers of the mouth, pharynx, and larynx and 1434 controls matched on age, gender, period of admission and study site. The association with dental factors was investigated by conditional logistic regression using extensive adjustment for a priori and empirical confounders, including tobacco and alcohol consumption, diet and sociodemographic variables. Lifetime use of dentures was not associated with risk of any UADT cancer, but history of oral sores secondary to ill-fitting dentures was associated with cancers of the mouth (odds ratio [OR] = 2.3, 95% confidence interval [CI] 1.2-4.6) and of the pharynx (OR = 2.7, 95% CI 1.1-6.2) among those using dentures. The association for mouth cancers was restricted primarily to an increased risk of tongue neoplasms (OR = 9.1, 95% CI 1.9-43.4). Less than daily tooth brushing frequency was also associated with risk of cancer of the tongue (OR = 2.1, 95% CI 1.0-4.3) and of other parts of the mouth (OR = 2.4, 95% CI 1.0-5.4). Having broken teeth was not significantly associated with risk of UADT cancer of any site. We conclude that poor oral hygiene due to infrequent tooth brushing and sores caused by dentures are risk factors for cancer of the mouth and that these associations are unlikely to be due to insufficient control of confounding.

Effects of four treatment strategies for temporomandibular joint closed lock.

A previous randomized controlled trial (RCT) by Schiffman et al. (2007)(15) compared four treatments strategies for temporomandibular joint (TMJ) disc displacement without reduction with limited mouth opening (closed lock). In this parallel group RCT, 106 patients with magnetic resonance imaging (MRI)-confirmed TMJ closed lock were randomized between medical management, non-surgical rehabilitation, arthroscopic surgery, and arthroplasty. Surgical groups also received rehabilitation post-surgically. The current paper reassesses the effectiveness of these four treatment strategies using outcome measures recommended by the International Association of Oral and Maxillofacial Surgeons (IAOMS). Clinical assessments at baseline and at follow-up (3, 6, 12, 18, 24, and 60 months) included intensity and frequency of TMJ pain, mandibular range of motion, TMJ sounds, and impairment of chewing. TMJ MRIs were performed at baseline and 24 months, and TMJ tomograms at baseline, 24 and 60 months. Most IAOMS recommended outcome measures improved significantly over time (P≤0.0003). There was no difference between treatment strategies relative to any treatment outcome at any follow-up (P≥0.16). Patient self-assessment of treatment success correlated with their ability to eat, with pain-free opening ≥35mm, and with reduced pain intensity. Given no difference between treatment strategies, non-surgical treatment should be employed for TMJ closed lock before considering surgery.

Searching for optimal dose-volume constraints to reduce rectal toxicity after hypofractionated radiotherapy for prostate cancer.

AIMS: Late rectal toxicity is a major concern for prostate cancer patients treated with radiotherapy. Rectal dose-volume constraints, set as guidelines to reduce its incidence, vary among institutions. From a group of patients uniformly treated with hypofractionated radiotherapy, we correlated the incidence of late rectal toxicity with rectal dose-volume rectal constraints as described in three randomised trials for prostate cancer. MATERIALS AND METHODS: Favourable-risk prostate cancer patients received a dose of 66 Gy in 22 fractions without hormonal therapy. Toxicity was prospectively assessed using Common Toxicity Criteria v3. The whole or part of the rectum and rectal wall were contoured as an organ at risk for all patients. The rectal constraints of the RTOG 0126, RTOG 0415 and the PROFIT trials were used to correlate with late rectal toxicity. RESULTS: The median follow-up time was 58 months. Late rectal toxicity was 62, 20 and 18% for grades 0, 1 and 2/3, respectively. No statistically significant correlation was found between late rectal toxicity and the rectal constraints used in the three trials. The number of patients violating the recommended constraints was similar for the group with grade 2/3 toxicity and the group without any toxicity. Analysis derived from the actual dose-volume histogram dose parameters of this group of patients did not show a relationship between dose to volume of the rectum and late rectal toxicity that could generate a guideline of dose constraints. CONCLUSION: For this group of patients, despite the use of recognised dose-volume constraint guidelines of three trials, we were unable to establish a relationship between these constraints and the late rectal toxicity registered. Further studies on the correlation of dosimetric parameters with rectal toxicity, particularly for hypofractionated regimens, are required. Non-dosimetric factors may also be involved in the risk of late rectal toxicity.

A case-control study of temporomandibular disorders: symptomatic disc displacement.

This case-control study was designed to investigate the risk factors for disc displacement (DD) without myofascial pain (MFP). The study population included 59 cases with DD without MFP, selected in two hospital dental clinics, and 100 concurrent controls selected in one of these clinics. The association with DD was evaluated for bruxism, head-neck trauma, orthodontic treatment, and sociodemographic characteristics by using unconditional logistic regression. In the multivariate analysis, excluding psychological factors, an association was found between DD and clenching-grinding (OR=3.57; 95% CI: 1.27-9.98). This association persisted when anxiety (OR=3.07; 95% CI: 1.08-8.70) or depression (OR=4.02; 95% CI: 1.43-11.31) was included in the model. A positive association was noted between orthodontic treatment and DD (OR=3.10; 95% CI: 1.06-9.65). The effect between orthodontic treatment and DD remained and increased with the inclusion of anxiety (OR=3.65; 95% CI: 1.15-11.61) or depression (OR=3.20; 95% CI: 1.06-9.65). A high level of anxiety (OR=2.40; 95% CI: 1.01-5.73), was positively related to DD. We concluded that clenching combined with grinding, and orthodontic treatment are factors related to DD. The interpretation of these associations, however, requires caution because of the inclusion of prevalent cases.

Heterogeneity of temporomandibular disorders: cluster and case-control analyses.

Cluster analysis has been applied to the classification of temporomandibular disorders (TMD). The factors most often used in these classification systems are psychological and psychosocial. The aim of this study is to classify individuals diagnosed with TMD, by using cluster analysis based on their clinical condition and the global severity of the disease. In this study, one investigator selected the patients at the dental clinics located at the Jewish General and Montreal General hospitals, Montreal, Canada, from September 1994 to December 1997. The study population included 162 outpatients. The results of this study indicated the existence of four TMD subgroups: three TMD pain groups with localized or generalized disorder related to different levels of interferences in their life and a non-pain, but disabled group. External validation of the cluster solution support the replication of the four groups and allow for further interpretation of the patients' profiles. Clenching-grinding and depression were related to the groups presenting generalized TMD. Orthodontic treatment and female sex, however, were the factors associated with a more localized condition. This classification system may provide a better understanding of the TMD subgroups and clues for the treatment and prognosis of TMD patients.