RESUMEN
A fractional factorial design approach has been used to enhance secondary metabolite production by two Penicillium strains. The method was initially used to improve the production of bioactive extracts as a whole and subsequently to optimize the production of particular bioactive metabolites. Enhancements of over 500% in secondary metabolite production were observed for both P. oxalicum and P. citrinum. Two new alkaloids, citrinalins A (5) and B (6), were isolated and identified from P. citrinum cultures optimized for production of minor metabolites.
Asunto(s)
Alcaloides/aislamiento & purificación , Antibacterianos/aislamiento & purificación , Penicillium/metabolismo , Alcaloides/química , Animales , Antibacterianos/química , Antibacterianos/farmacología , Candida albicans/efectos de los fármacos , Caulerpa/microbiología , Cromatografía Líquida de Alta Presión , Cristalografía por Rayos X , Escherichia coli/efectos de los fármacos , Femenino , Humanos , Biología Marina , Staphylococcus aureus Resistente a Meticilina/efectos de los fármacos , Ratones , Pruebas de Sensibilidad Microbiana , Conformación Molecular , Estructura Molecular , Mycobacterium tuberculosis/efectos de los fármacos , Resonancia Magnética Nuclear Biomolecular , Penicillium/química , Pseudomonas aeruginosa/efectos de los fármacos , Proyectos de InvestigaciónRESUMEN
Chemical investigation of the cytotoxic and anti-tuberculosis active butanone extract obtained from the growth media of the marine-derived fungus Beauveria felina led to the isolation of two new destruxins, [beta-Me-Pro] destruxin E chlorohydrin (1) and pseudodestruxin C (3), along with five known cyclic depsipeptides. The structures of the new destruxin derivatives were established by analysis of spectroscopic data, while the absolute configuration of the common amino acid residues was established by Marfey's analysis. The absolute configuration of the 2(R),4(S)-5-chloro-2,4-dihydroxypentanoic acid residue in 1 could be established by application of a J-based configuration method followed by derivatization with R-MPA-Cl and NMR analysis.