RESUMEN
We describe a case of a testicular primitive neuroectodermal tumor (PNET) with intratubular germ cell neoplasia of the adjacent testicular parenchyma. The occurrence of testicular PNET is rare because malignant transformation of testicular germ cell tumors into somatic malignancy is uncommon. Based on morphological, immunohistochemical and molecular pathological findings these tumors resemble central PNETs as they otherwise typically occur in children without rearrangement of the Ewing sarcoma breakpoint region (EWSR) gene on chromosome 22. This case also showed no evidence for a translocation.
Asunto(s)
Biomarcadores de Tumor/genética , Transformación Celular Neoplásica/genética , Transformación Celular Neoplásica/patología , Neoplasias de Células Germinales y Embrionarias/genética , Neoplasias de Células Germinales y Embrionarias/patología , Neoplasias Testiculares/genética , Neoplasias Testiculares/patología , Adulto , Proteínas de Unión a Calmodulina/genética , Carcinoma in Situ/genética , Carcinoma in Situ/patología , Carcinoma in Situ/cirugía , Cromosomas Humanos Par 21/genética , Cromosomas Humanos Par 22/genética , Reordenamiento Génico/genética , Humanos , Hibridación Fluorescente in Situ , Antígeno Ki-1/genética , Masculino , Neoplasias de Células Germinales y Embrionarias/cirugía , Tumores Neuroectodérmicos Periféricos Primitivos/cirugía , Factor 3 de Transcripción de Unión a Octámeros/genética , Orquiectomía , Proteína EWS de Unión a ARN , Proteínas de Unión al ARN/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Neoplasias Testiculares/cirugía , Testículo/patología , Translocación Genética/genéticaRESUMEN
BACKGROUND: P150, a 150 kDa protein, was isolated from virally and oncogene-transformed mouse cell lines, partially purified and cloned. P150 is part of the large subunit of the eukaryotic translation initiation factor 3 with sequence homology to centrosomin A. A significant correlation between p150 expression and malignancy in breast, cervical and esophageal cancer have recently been demonstrated. MATERIALS AND METHODS: Here, 110 colorectal carcinomas of different grades and stages, including lymph node and liver metastases were compared to adjacent normal mucosa by immunohistochemistry of P150. Western blot analysis of selected cases confirmed the expression levels determined by immunohistochemistry. Additionally, immuno-electron and laser scanning microscopy (LSM) was performed. RESULTS: All investigated carcinomas revealed high levels of p150 protein compared to normal adjacent mucosa. The staining intensity was slightly heterogeneous, and positivity was correlated to the tumor grade with statistically significant differences of p150 expression between normal and neoplastic mucosa (p<0.0001, Kruskal-Wallis test). Western blots confirmed higher expression levels of p150 in the tumor. Immunogold labelling and LSM investigation showed high expression levels of p150 on the rough endoplasmic reticulum and polyribosomes, indicating that p150 is translationally active in these tumors. CONCLUSION: Thus, we propose that p150 plays an important role in development and growth of colorectal carcinomas. Furthermore, p150 expression might provide us with reliable information on the biological behaviour of tumors and the clinical course of the disease.