RESUMEN
Single-atom catalysts (SACs) are appealing for carbon dioxide (CO2) electroreduction with the utmost advantages; however, their preparation is still challenging because of the complicated procedure. Here, a novel Ni-based single-atom catalyst (Ni-BB-BD) is constructed from raw materials, [BMIM]BF4, [BMIM]DCN, and NiCl2·6H2O, directly without any precursor by only one-step pyrolysis. Ni-BB-BD achieves a maximum carbon monoxide Faradaic efficiency (FECO) of 96.5% at -0.8 V vs RHE, as well as long-term stability over 16 h. High current density up to -170.6 mA cm-2 at -1.0 V vs RHE is achieved in the flow cell along with a CO selectivity of 97.7%. It is identified that [BMIM]BF4 is the nitrogen source, while [BMIM]DCN is mainly taken as the carbon source. Theoretical studies have revealed that the rich nitrogen content, especially for the uncoordinated nitrogen, plays a critical role in lowering rate-limiting barrier height. This work develops a facile and effective strategy to prepare the SACs.
RESUMEN
Botryosphaeria dothidea is a worldwide pathogenic fungus that causes stem canker, leaf dieback, and fruit rot on a large number of crops and trees. Gummosis caused by B. dothidea is one of the most prevalent and devastating diseases on peach in southern China. This study reported a high-quality and well-annotated genome sequence of B. dothidea strain XNHG241. The findings can be used as a reference for studying fungal biology, pathogenic mechanism of B. dothidea, and the interaction between B. dothidea and host, and eventually facilitate peach gummosis management.
Asunto(s)
Ascomicetos , Enfermedades de las Plantas , Enfermedades de las Plantas/microbiología , Ascomicetos/genética , ChinaRESUMEN
14-3-3 proteins are signal moderators in sensing various stresses and play essential functions in plant growth and development. Although, 14-3-3 gene families have been identified and characterized in many plant species, its evolution has not been studied systematically. In this study, the plant 14-3-3 family was comprehensively analyzed from green algae to angiosperm. Our result indicated that plant 14-3-3 originated during the early evolutionary history of green algae and expanded in terricolous plants. Twenty-six 14-3-3 genes were identified in the tea genome. RNA-seq analysis showed that tea 14-3-3 genes display different expression patterns in different organs. Moreover, the expression of most tea 14-3-3 genes displayed variable expression patterns under different abiotic and biotic stresses. In conclusion, our results elucidate the evolutionary origin of plant 14-3-3 genes, and beneficial for understanding their biological functions and improving tea agricultural traits in the future.
Asunto(s)
Camellia sinensis , Camellia sinensis/genética , Camellia sinensis/metabolismo , Perfilación de la Expresión Génica/métodos , Regulación de la Expresión Génica de las Plantas , Filogenia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Estrés Fisiológico/genética , Té/genética , Té/metabolismoRESUMEN
OBJECTIVES: Electroconvulsive therapy (ECT) has significant effects on improving psychotic symptoms in schizophrenia (SZ), but the changes of brain function induced by it are unclear. The purpose of the study was to explore progressive ECT-induced changes in regional homogeneity (ReHo) at multiple time points before, during, and after a course of ECT. METHODS: The 27 in-patients with SZ (SZ group) who met the recruitment criteria accepted clinical evaluations and resting-state functional magnetic resonance imaging scans before the first ECT (pre-ECT), after the first ECT (ECT1), and after the eighth ECT (ECT8), all conducted within 10 to 12 hours. Forty-three healthy controls (HCs; HC group) who matched well with the patients for age, sex, and years of education were recruited. For Positive and Negative Syndrome Scale (PANSS) and ReHo, progressive changes were examined. RESULTS: Pair-wise comparisons of patient pre-ECT, ECT1, and ECT8 ReHo values with HC ReHo values revealed that ECT normalized the ReHo values in bilateral superior occipital gyrus (SOG), right lingual gyrus (LG), left medial prefrontal cortex. Furthermore, improved ReHo in bilateral SOG and right LG appeared after the first ECT application. The ReHo values in right middle occipital gyrus, right middle temporal gyrus, and right inferior parietal lobule were not significantly altered by ECT. The total PANSS score was lower even after the first ECT application (mean ΔPANSSECT1, 11.7%; range, 2%-32.8%) and markedly reduced after the eighth application (mean ΔPANSSECT8, 86.3%; range, 72.5%-97.9%). CONCLUSIONS: The antipsychotic effects of ECT may be achieved through regulating synchronization of some regions such as bilateral SOG, right LG, and left medial prefrontal cortex. Furthermore, the enhanced synchronizations also take place in other regions.
Asunto(s)
Terapia Electroconvulsiva , Esquizofrenia , Encéfalo/diagnóstico por imagen , Mapeo Encefálico , Terapia Electroconvulsiva/métodos , Humanos , Imagen por Resonancia Magnética/métodos , Esquizofrenia/terapiaRESUMEN
The formosolv fractionation process has been demonstrated to be an effective approach toward lignin recovery as an antioxidant from lignocellulosic biomass. In this study, four lignin fractions, FL-88%, FSL-70%, FIL-70% and FL-EtAc, were isolated from Phragmites australis biomass through two-step formosolv fractionation (88% formic acid delignification followed by 70% aqueous formic acid fractionation). To better understand the structural properties of the lignin obtained from this fractionation process, four isolated lignins were successfully characterized by gel permeation chromatography (GPC), Fourier transform infrared (FT-IR), two-dimensional heteronuclear single quantum coherence nuclear magnetic resonance (2D-HSQC NMR) spectroscopy, thermogravimetric analysis (TGA) and gas chromatograph-mass spectroscopy (GC/MS). It was found that lignin depolymerization via ß-O-4 cleavage occurred via a formylation, elimination and hydrolysis mechanism, accompanied by a competitive condensation reaction. Noteworthily, two-step formosolv fractionation can produce specific lignin fractions with different ABTS and DPPH radical scavenging activities. The FL-EtAc fraction with low molecular weight (Mw = 2748 Da) and good homogeneity (PDI = 1.5) showed excellent antioxidant activity, compared with the other three isolated lignin fractions, even equal to that of commercial antioxidant BHT at the same concentration of 2.0 mg·mL-1. These findings are of great help for specific lignin from biomass as a natural antioxidant in the future.
Asunto(s)
Antioxidantes , Lignina , Antioxidantes/química , Fraccionamiento Químico/métodos , Hidrólisis , Lignina/química , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
Previous biomechanics studies suggest that higher cognitive mental workload when performing office computer tasks may increase the risk of MSDs among office workers. Cognitive workload can be interpreted in terms of task factors (e.g. task complexity and time pressure) and mental workload factors which include mental demand and mental effort. A laboratory study was conducted to further explore how the task and mental workload factors affected computer users' biomechanical responses, specifically the muscle activation levels and sitting postures. Data were collected as 20 participants worked on computer tasks which varied in their levels of task complexity and time pressure. Visual analog scales were used for assessing mental workload factors. Results indicated that the level of mental effort reported, as opposed to the level of task complexity, was associated with changes in participants' biomechanical responses, but primarily occurred when the chair's backrest was not used. Practitioner summary: A study was conducted to investigate the association between computer users' cognitive workload and biomechanical responses when performing computer task. While task complexity was not directly associated with the changes in participants' biomechanical responses, higher reported mental effort was associated with increased biomechanical responses, but only when the participants did not use the backrest on the chair.
Asunto(s)
Análisis y Desempeño de Tareas , Carga de Trabajo , Computadores , Humanos , Postura , Sedestación , Carga de Trabajo/psicologíaRESUMEN
The current study investigated the impact of cognitive flexibility on the development of emotion understanding using a longitudinal tracking study. A total of 98 children aged 4 and 5 years were tested for cognitive flexibility, emotion understanding, and verbal ability across three time points within a year. The cross-lagged analyses indicated that early cognitive flexibility played a predictive role in the development of emotion understanding. More precisely, cognitive flexibility at Time 1 predicted emotion understanding at Time 2 and Time 3, and cognitive flexibility at Time 2 predicted emotion understanding at Time 3. Furthermore, mediation analysis showed that verbal ability mediated the impact of cognitive flexibility on emotion understanding. Early cognitive flexibility contributed to later emotion understanding by improving children's verbal ability. These findings suggest that there is a verbal ability-mediated pathway from cognitive flexibility to emotion understanding that provides a new perspective for the development mechanism of children's emotion understanding.
Asunto(s)
Cognición , Emociones , Niño , Desarrollo Infantil , Humanos , Estudios LongitudinalesRESUMEN
Oncolytic Newcastle disease virus (NDV) induces immunogenic cell death (ICD), liberating danger-associated molecular patterns (DAMPs) that provokes defiance in neoplastic malignancy. The present study aims to investigate whether and how oncolytic NDV triggers ICD in prostate cancer cells. We show that NDV/FMW, an oncolytic NDV strain FMW, elicited the expression and release of several ICD markers, that is calreticulin (CRT), heat shock proteins (HSP70/90) and high-mobility group box 1 (HMGB1), in prostate cancer cells. Furthermore, pharmacological repression of apoptosis, necroptosis, autophagy or endoplasmic reticulum (ER) stress exerted diverse effects on the HMGB1 and HSP70/90 evacuation in NDV/FMW-infected prostate cancer cells. Moreover, ICD markers induced in prostate cancer cells upon NDV/FMW infection, were enhanced by either treatment with a STAT3 (signal transducer and activator of transcription 3) inhibitor or shRNA-mediated knockdown of STAT3. In nude mice bearing prostate cancer cell-derived tumours, the tumours injected with the supernatants of NDV/FMW-infected cells grew smaller than mock-treated tumours. These results indicate that oncolytic NDV provokes the expression of ICD makers in prostate cancer cells. Our data also suggest that a combination of inhibition of STAT3 with oncolytic NDV could boost NDV-based anti-tumour effects against prostate cancer.
Asunto(s)
Muerte Celular Inmunogénica/genética , Viroterapia Oncolítica , Neoplasias de la Próstata/genética , Factor de Transcripción STAT3/genética , Animales , Apoptosis/genética , Autofagia/genética , Calreticulina/genética , Línea Celular Tumoral , Estrés del Retículo Endoplásmico/genética , Regulación Neoplásica de la Expresión Génica/genética , Proteína HMGB1/genética , Proteínas HSP70 de Choque Térmico/genética , Proteínas HSP90 de Choque Térmico/genética , Humanos , Masculino , Ratones , Ratones Desnudos , Necroptosis/genética , Virus de la Enfermedad de Newcastle/genética , Virus Oncolíticos/genética , Neoplasias de la Próstata/terapia , Neoplasias de la Próstata/virología , Factor de Transcripción STAT3/antagonistas & inhibidores , Ensayos Antitumor por Modelo de XenoinjertoRESUMEN
OBJECTIVE: To explore the change of muscular and biomechanical responses in different load stability and visual access conditions during an asymmetric lifting task. BACKGROUND: Previous studies found that lifting unstable loads resulted in changes to the biomechanical loads experienced by the spine and upper extremities. However, researchers have not extensively investigated behaviors when people lift potentially unstable loads. It was hypothesized that lifting a potentially unstable load can lead to changes in lifting behavior, which may be mitigated by visual access to the load. METHOD: Fourteen volunteers lifted either a stable load or a potentially unstable load that could move within the container during the lifting task. In half of the lifting conditions, the box was covered to restrict visual access when lifting. Spine kinematic and kinetic measures and surface electromyographic (EMG) signals from back, shoulder, and arm muscles were obtained. RESULTS: Lifts of the stable load were faster and generally had higher peak muscle activations than lifts of the potentially unstable load. Participants had less spine flexion when handling the potentially unstable load without visual access. CONCLUSION: When lifting and moving a potentially unstable load that could lead to a perturbation, people tended to lift the container more slowly comparing with lifting a stable load, which in turn reduced the peak muscle activities. APPLICATION: In industry, there are many work situations where workers need to lift or carry unstable loads that can shift during transport. Providing visual access to the load may help mitigate some of these effects.
Asunto(s)
Elevación , Extremidad Superior , Percepción Visual , Soporte de Peso , Fenómenos Biomecánicos , Electromiografía , Femenino , Humanos , Masculino , Adulto JovenRESUMEN
This paper aims to investigate the relationship between ESG performance and corporate innovation using a sample of Chinese-listed companies from 2009 to 2021. The findings reveal that ESG performance is positively correlated with both the quantity and quality of corporate innovation. Further, we employed the causal step approach and the Sobel mediation effect test to empirically examine the mechanisms. The results support that ESG performance promotes corporate innovation by reducing agency problems, enhancing information disclosure, and improving internal corporate governance. Heterogeneity analysis indicates that the positive impact of ESG performance on corporate innovation is more pronounced among firms in the central and western regions, as well as those in heavily polluting industries. Notably, we corrected for the truncation-bias in the patent data. This study contributes to the expanding literature on the economic implications of ESG and holds policy implications for promoting the high-quality development of corporate innovation.
RESUMEN
Cultivating strategic emerging industries (SEIs) is an important strategy for most countries around the world to seize the economic frontier. Academics have not yet reached a unified conclusion on whether the adoption of industrial policy from the government level can effectively promote its R&D and innovation behaviors and contribute to industrial upgrading. Based on the data regarding 33,425 Shanghai and Shenzhen A-share-listed companies from 2007 to 2020, this article employs the difference-in-difference model (DID) and the mediated effect model to identify the effect and mechanism of how industrial policy affects the innovation behavior of SEIs. The results of this study show that the promulgation and implementation of industrial policies can help stimulate enterprises to carry out R&D and innovation behaviors and improve the innovation level of SEIs. Its promoting effect on state-owned enterprises is more significant than that on non-state-owned enterprises, and its promoting effect on the eastern and central regions is more significant than that on the western region. Further analysis reveals that government subsidies and tax incentives are important transmission mechanisms through which industrial policy affects firms' innovation, with government subsidies playing a positive facilitating role and tax incentives having a negative impact.
RESUMEN
Electrochemical carbon dioxide reduction reaction (eCO2RR) to generate syngas is an appealing strategy for CO2 net reduction. However, it suffers from the inferior faradaic efficiency (FE), selectivity, and difficult modulation of hydrogen/carbon monoxide (H2/CO) ratio. To address these issues, a series of magnesium-nickel (Mg-Ni) dual atomic catalysts with different Ni contents are fabricated on the nitrogen-doped carbon matrix (MgNiX-NC DACs) by one-step pyrolysis. MgNi5-NC electrocatalyst generates 0.51-0.79 H2/CO ratios in a potential range of -0.6 to -1.0 V vs. reversible hydrogen electrode (RHE) and the total FE reaches 100 % with good stability. While a wider range of H2/CO (0.95-4.34) is achieved for MgNi3-NC electrocatalyst in the same overpotential range, which is suitable for typical downstream thermochemical reactions. Introduction of Ni species accelerates the generation of CO, however, there is much less influence on the H2 production as compared with Mg-based single atomic electrocatalyst. According to the experimental results and density functional theory (DFT) calculations, the synergistic effect between Mg and Ni achieves the satisfied results rather than each one fulfill its own duty for selective producing H2 and CO, respectively. This work introduces a feasible approach to develop atomic catalysts on main group metal for more controllable CO2RR.
RESUMEN
This study aimed to explore the effects of different nitrogen, phosphorus, and potassium ratios on the yield and nutritional quality of greenhouse tomatoes under a water and fertilizer integration model. Greenhouse tomatoes were used as the research object, and the "3414" fertilizer trial design was employed to assess tomato growth, yield, quality, and soil indicators across various treatment combinations. The goal was to determine the optimal fertilization scheme and recommend appropriate fertilizer quantities for tomato cultivation and production. The results revealed that different fertilizer ratios significantly affected both the quality and yield of tomatoes. Overall, the tomato yield tended to increase with higher fertilization amounts, with potassium exhibiting the most pronounced effect on yield increase, followed by phosphorus and nitrogen. The comprehensive analysis of principal components indicated that the N2P2K1 treatment yielded the highest nutritional quality and yield. Therefore, the best fertilization combination identified in this study consisted of nitrogen fertilizer at 197.28 kg hm-2, phosphorus fertilizer at 88.75 kg hm-2, and potassium fertilizer at 229.80 kg hm-2. These findings provided the scientific basis for optimizing fertilization practices in greenhouse tomato cultivation and production in the Jilin Province.
RESUMEN
BACKGROUND: Studies have shown that the chronic use of cannabis is associated with a decrease in blood pressure. Our previous studies prove that activating the cannabinoid type 2 (CB2) receptor in the brain can effectively reduce blood pressure in spontaneously hypertensive rats; however, the exact mechanism has not been clarified. The objective of this study is to demonstrate that activation of microglial CB2 receptors can effectively reduce the levels of TNF-α, IL-1ß, and IL-6 in the paraventricular nucleus (PVN) through inhibiting aerobic glycolysis, thereby relieving hypertension. METHODS: AngiotensinII (AngII) was administered to BV2 cells and C57 mice to induce hypertension and the release of proinflammatory cytokines. The mRNA and protein expression of the CB2 receptor, TNF-α, IL-1ß, IL-6, and the PFK and LDHa enzymes were detected using RT-qPCR and Western blotting. The Seahorse XF Energy Metabolism Analyzer was used to measure the oxidative phosphorylation and aerobic glycolysis metabolic pathways in BV2 cells. The long-term effects of injecting JWH133, a selective CB2 receptor agonist, intraperitoneally on blood pressure were ascertained. ELISA was used to measure norepinephrine and lactic acid levels while immunofluorescence labeling was used to locate the CB2 receptor and c-Fos. By injecting pAAV-F4/80-GFP-mir30shRNA (AAV2-r-CB2shRNA) into the lateral cerebral ventricle, the CB2 receptor in microglia was specifically knocked down. RESULTS: Activation of CB2 receptors by the agonist JWH133 suppressed TNF-α, IL-1ß, and IL-6 by inhibiting PFK and LDHa enzymes involved in glycolysis, as well as lactic acid accumulation, along with a reduction in glycoPER levels (marks of aerobic glycolysis) in AngII-treated BV2 cells. In AngII-treated mice, the administration of JWH133 specifically activated CB2 receptors on microglia, resulting in decreased expression levels of PFK, LDHa, TNF-α, IL-1ß, and IL-6, subsequently leading to a decrease in c-Fos protein expression within PVN neurons as well as reduced norepinephrine levels in plasma, ultimately contributing to blood pressure reduction. CONCLUSION: The results suggest that activation of the microglia CB2 receptor decreases the neuroinflammation to relieve hypertension; the underlying mechanism is related to inhibiting aerobic glycolysis of microglia.
Asunto(s)
Cannabinoides , Hipertensión , Receptor Cannabinoide CB2 , Animales , Ratones , Ratas , Agonistas de Receptores de Cannabinoides/farmacología , Cannabinoides/farmacología , Glucólisis , Hipertensión/tratamiento farmacológico , Hipertensión/metabolismo , Interleucina-6/metabolismo , Ácido Láctico/metabolismo , Microglía/metabolismo , Enfermedades Neuroinflamatorias , Norepinefrina/metabolismo , Ratas Endogámicas SHR , Receptor Cannabinoide CB2/efectos de los fármacos , Receptor Cannabinoide CB2/genética , Receptor Cannabinoide CB2/metabolismo , Factor de Necrosis Tumoral alfa/metabolismoRESUMEN
Ammonia monohydrate (NH3·H2O) is an important chemical widely used in industrial, agricultural, and pharmaceutical fields. Reject water is used as the raw material in self-built bipolar membrane electrodialysis (BMED) to produce NH3·H2O. The effects of electrode materials, membrane stack structure, and operating conditions (current density, initial concentrations of the reject water, and initial volume ratio) on the BMED process were investigated, and the economic costs were analyzed. The results showed that compared with graphite electrodes, ruthenium-iridium-titanium electrodes as electrode plates for BMED could increase current efficiency (25%) and reduce energy consumption (26%). Compared with two-compartment BMED, three-compartment BMED had a higher ammonia nitrogen conversion rate (86.6%) and lower energy consumption (3.5â kW· h/kg). Higher current density (15â mA/cm2) could achieve better current efficiency (79%). The BMED performances were improved when the initial NH4+ concentrations of the reject water increased from 500â mg NH4+/L to 1000â mg NH4+/L, but the performance decreased as the concentration increased from 1000â mg NH4+/L to 1500â mg NH4+/L. High initial volume ratio of the salt compartment and product compartment was beneficial for reducing energy consumption. Under the optimal operating conditions, only 0.13 $/kg reject water was needed to eliminate the environmental impact of reject water accumulation. This work indicates that BMED can not only achieve desalination of reject water, but also generate products that alleviate the operational pressure of factories.
RESUMEN
As a new technology for accurate utilization of sludge resources, sludge inorganic-organic matter separation (IOMS) has attracted wide attention. This study examined the impact of this pretreatment on environmental and economic performance of sludge composting and incineration using life cycle assessment (LCA) and whole life costing (WLC). LCA results indicated that IOMS pretreatment reduced the energy conservation and emission reduction (ECER) values of composting and incineration by 56 % and 76 %, respectively. Meanwhile, WLC exhibited that IOMS pretreatment could cut the break-even year of incineration from 11 years to 4 years. The combination of organic sludge incineration/composting with inorganic sludge sintering ceramsite reveals excellent environmental and economic performance. The application optimization hypothesis analysis of these two routes in various provinces of China indicates that Jiangsu has the greatest development potential and should become a major promotion region.
Asunto(s)
Aguas del Alcantarillado , Compostaje/métodos , Incineración , Ambiente , ChinaRESUMEN
During task-based functional magnetic resonance imaging (t-fMRI) patients with depressive disorder (DD) have shown abnormal caudate nucleus activation. There have been no meta-analyses that are conducted on the caudate nucleus using Activation Likelihood Estimation (ALE) in patients with DD, and the relationships between abnormal caudate activity and different behavior domains in patients with DD remain unclear. There were 24 previously published t-fMRI studies included in the study with the caudate nucleus as the region of interest. Meta-analyses were performed using the method of ALE. Included five ALE meta-analyses: (1) the hypoactivated caudate nucleus relative to healthy controls (HCs); (2) the hyper-activated caudate nucleus; (3) the abnormal activation in the caudate nucleus in the emotion domain; (4) the abnormal activation in cognition domain; (5) the abnormal activation in the affective cognition domain. Results revealed that the hypo-/hyper-activity in the caudate subregions is mainly located in the caudate body and head, while the relationships between abnormal caudate subregions and different behavior domains are complex. The hypoactivation of the caudate body and head plays a key role in the emotions which indicates there is a positive relationship between the decreased caudate activity and depressed emotional behaviors in patients with DD.
Asunto(s)
Núcleo Caudado , Trastorno Depresivo , Humanos , Núcleo Caudado/diagnóstico por imagen , Encéfalo , Emociones/fisiología , Trastorno Depresivo/diagnóstico por imagen , Imagen por Resonancia Magnética/métodosRESUMEN
Metabolic-associated fatty liver disease (MAFLD) is a chronic liver disease characterized by the excessive accumulation of fat in hepatocytes. However, due to the complex pathogenesis of MAFLD, there are no officially approved drugs for treatment. Therefore, there is an urgent need to find safe and effective anti-MAFLD drugs. Recently, the relationship between the gut microbiota and MAFLD has been widely recognized, and treating MAFLD by regulating the gut microbiota may be a new therapeutic strategy. Natural products, especially plant natural products, have attracted much attention in the treatment of MAFLD due to their multiple targets and pathways and few side effects. Moreover, the structure and function of the gut microbiota can be influenced by exposure to plant natural products. However, the effects of plant natural products on MAFLD through targeting of the gut microbiota and the underlying mechanisms are poorly understood. Based on the above information and to address the potential therapeutic role of plant natural products in MAFLD, we systematically summarize the effects and mechanisms of action of plant natural products in the prevention and treatment of MAFLD through targeting of the gut microbiota. This narrative review provides feasible ideas for further exploration of safer and more effective natural drugs for the prevention and treatment of MAFLD.
Asunto(s)
Productos Biológicos , Microbioma Gastrointestinal , Enfermedad del Hígado Graso no Alcohólico , Humanos , Enfermedad del Hígado Graso no Alcohólico/tratamiento farmacológico , Productos Biológicos/farmacología , Productos Biológicos/uso terapéutico , HepatocitosRESUMEN
Concurrent changing precipitation regimes and atmospheric nitrogen (N) deposition can have profound influences on soil carbon (C) cycling. However, how N enrichment regulates the responses of soil C fluxes to increasing variability of precipitation remains elusive. As part of a field precipitation gradient experiment with nine levels of precipitation amounts (-60 %, -45 %, -30 %, -15 %, ambient precipitation, +15 %, +30 %, +45 %, and +60 %) and two levels of N addition (0 and 10 g N m-2 yr-1) in a semi-arid temperate steppe on the Mongolian Plateau, this work was conducted to investigate the responses of soil respiration to decreased and increased precipitation (DP and IP), N addition, and their possible interactions. Averaged over the three years from 2019 to 2021, DP suppressed soil respiration by 16.1 %, whereas IP stimulated it by 27.4 %. Nitrogen addition decreased soil respiration by 7.1 % primarily via reducing microbial biomass C. Soil respiration showed symmetric responses to DP and IP within all the four precipitation variabilities (i.e., 15 %, 30 %, 45 %, and 60 %) under ambient N. Nevertheless, N addition did not alter the symmetric responses of soil respiration to changing precipitation due to the comparable sensitivities of microbial biomass and root growth to DP and IP under the N addition treatment. These findings indicate that intensified precipitation variability does not change but N addition could alleviate soil C releases. The unchanged symmetric responses of soil respiration to precipitation variability under N addition imply that N deposition may not change the response pattern of soil C releases to predicted increases in precipitation variability in grasslands, facilitating the robust projections of ecosystem C cycling under future global change scenarios.