Construction of and evaluation of the immune response to two recombinant pseudorabies viruses expressing the B119L and EP364R proteins of African swine fever virus.

African swine fever (ASF) is an infectious disease caused by ASF virus (ASFV), which is characterized by high infectivity, rapid onset of disease, and a high mortality rate. Outbreaks of ASFV have caused great economic losses to the global pig industry, and there is a need to develop safe and effective vaccines. In this study, two recombinant pseudorabies virus (PRV) strains, rGXGG-2016-ΔgI/ΔgE-EP364R and rGXGG-2016-ΔgI/ΔgE-B119L, expressing the EP364R and B119L protein, respectively, of ASFV, were constructed by homologous recombination technology. Western blotting and immunofluorescence analysis showed that these foreign proteins were expressed in cells infected with the recombinant strains. The strains showed good genetic stability and proliferative characteristics for 20 passages in BHK-21 cells. Both of these strains were immunogenic in mice, inducing the production of specific antibodies against the expressed ASFV proteins while providing protection against lethal challenge with PRV. Thus, the recombinant strains rGXGG-2016-ΔgI/ΔgE-EP364R and rGXGG-2016-ΔgI/ΔgE-B119L could be used as candidate vaccines for both ASFV and PRV. In addition, our study identifies two potential target genes for the development of safe and efficient ASFV vaccines, provides a reference for the construction of bivalent ASFV and PRV vaccines, and demonstrates the feasibility of developing a live ASFV vector vaccine.

Exploring watershed ecological risk bundles based on ecosystem services: A case study of Shanxi Province, China.

Humans are having an increasingly profound impact on the environment along with the advent of the Anthropocene. Ecological risk assessment (ERA) as a method to quantify ecological problems can provide support for decision-makers, and it is one of key issues to integrate ecosystem services into ERA. In this study, an ERA framework was proposed under the loss-probability paradigm from the perspective of ecosystem services risk bundles. The results showed that initiatives aimed at ecological protection in Shanxi Province had been effective, the number of watersheds with low-risk bundles increased significantly (from 16.09% to 34.49%) and the watersheds basically overlapped with key forestation areas. However, the effects of forestation activities may no longer be as significant as they once were, as the relationship between forestation and water supply was becoming increasingly contradictory. Meanwhile, the conflict between urban expansion and natural ecosystem protection was intensifying, habitat degradation risks were gradually polarized, and the risk bundles dominated by high carbon emission and habitat degradation were increasing significantly (from 15.88% to 33.54%). Strengthening the construction of urban green space and controlling the expansion of human activities may be the next focus of ecological conservation in Shanxi Province. This study enriched the ERA framework with an ecosystem services risk bundle approach.

The Effects of Angelica Sinensis Polysaccharide on Tumor Growth and Iron Metabolism by Regulating Hepcidin in Tumor-Bearing Mice.

BACKGROUND/AIMS: Iron plays a fundamental role in cell biology and its concentration must be precisely regulated. It is well documented that excess iron burden contributes to the occurrence and progression of cancer. Hepcidin secreted by liver plays an essential role in orchestrating iron metabolism. In the present study, we aimed to investigate the ability of angelica sinensis polysaccharide (ASP) to decrease iron burden in tumor-bearing mice and the mechanism of ASP regulation hepcidin expression. METHODS: Western blot, RT-PCR, immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA) were used to detect the regulation of hepcidin and related cytokines by ASP. The role of ASP in tumor proliferation was investigated using in vivo assays. Iron depositions and iron concentrations in organs were determined by hematoxylin-eosin (H&E) staining and atomic absorption spectrophotometer. RESULTS: We found that ASP could inhibit tumor growth in mice xenografted with 4T1 and H22 cancer cells. In vivo experiments also showed that ASP could potently regulate hepcidin expression in liver and serum and decrease iron burden in liver, spleen and grafted tumors in mouse model. Treatment with ASP in hepatic cell lines reproduced comparable results in decreasing hepcidin as in mouse liver. Furthermore, we found that ASP markedly suppressed the expression of interleukin-6 (IL-6), JAK2, p-STAT3, and p-SMAD1/5/8 in liver, suggesting that JAK/STAT and BMP-SMAD pathways were involved in the regulation of hepcidin expression by ASP. We also found down-regulation of iron-related cytokines in ASP treated mice. CONCLUSION: The present study provides new evidence that ASP decreases hepcidin expression, which can reduce iron burden and inhibit tumor proliferation. These findings might aid ASP developed as a potential candidate for cancer treatment in patients with iron overload.

Nitrogen Emissions-Based Assessment of Anthropogenic Regional Ecological Risk: An Example of Taiwanese Urbanization, 1990-2015.

This study proposes a framework for evaluating anthropogenic nitrogen emissions and local vulnerability in order to assess regional ecological risk of human activity during a stable urbanization process. Taiwan, an isolated island with a unique environment, intensive agriculture, concentrated industries, and stable urbanization, was an ideal location for testing this framework. Local vulnerability is influenced by social characteristics, economic development, environmental protection, and other indicators related to these. Within the context of urbanization, therefore, and using official statistical data, human metabolism, agricultural and industrial production, and transportation were evaluated. The results indicate that the rate of anthropogenic nitrogen emissions decreased as the process of urbanization in Taiwan stabilized. While nitrogen emissions from agricultural production, household and industrial wastewater gradually decreased due to a reduction in the area of arable farmland, a reduction in the use of fertilizers and increased sewage treatment, nitrogen emissions from transportation increased due to higher energy consumption from vehicle use. Taiwan exhibited a higher degree of regional vulnerability in 1998 because motor vehicle density increased significantly, while rates of per capita green area and resource recovery remained relatively low. The study found that if Taiwan maintains its current conditions with respect to standard of living, agriculture, industry, and transportation, nitrogen emissions from human metabolism and agricultural and industrial production will not increase regional ecological risk, while nitrogen emissions from transportation will likely increase this risk. Therefore, this paper suggests that future environmental planning in Taiwan should prioritize low-emissions sustainable transportation.

Analyzing nonlinear variations in terrestrial vegetation in China during 1982-2012.

Quantifying the long-term trends of changes in terrestrial vegetation on a large scale is an effective method for detecting the effects of global environmental change. In view of the trend towards overall restoration and local degradation of terrestrial vegetation in China, it is necessary to pay attention to the spatial processes of vegetative restoration or degradation, as well as to clarify the temporal and spatial characteristics of vegetative growth in greater geographical detail. However, traditional linear regression analysis has some drawbacks when describing ecological processes. Combining nonparametric linear regression analysis with high-order nonlinear fitting, the temporal and spatial characteristics of terrestrial vegetative growth in China during 1982-2012 were detected using the third generation of Global Inventory Modeling and Mapping Studies (GIMMS3g) dataset. The results showed that high-order curves could be effective. The region joining Ordos City and Shaanxi Gansu Ningxia on the Loess Plateau may have experienced restoration-degradation-restoration processes of vegetative growth. In the Daloushan Mountains, degradation-restoration processes of vegetative growth may have occurred, and the occurrence of several hidden vegetative growth processes was located in different regions of eastern China. Changes in cultivated vegetation were inconsistent with changes in other vegetation types. In southern China and some high-altitude areas, temperature was the primary driver of vegetative growth on an interannual scale, while in the north, the effect of rainfall was more significant. Nevertheless, the influence of climate on vegetation activity in large urban areas was weak. The trend types of degradation-restoration processes in several regions were inconsistent with the implements of regional land development and protection strategy. Thus, the role of human activity cannot be ignored. In future studies, it will be still necessary to quantify the effects of human management on spatial patterns, develop trend-fitting methods, and explore more refined methods of analyzing the driving forces affecting large-scale changes in vegetative growth.

Isolation and identification of a novel porcine-related recombinant mammalian orthoreovirus type 3 strain from cattle in Guangxi Province, China.

The Mammalian orthoreovirus (MRV) infects various mammals, including humans, and is linked to gastrointestinal, respiratory, and neurological diseases. A recent outbreak in Liuzhou, Guangxi, China, led to the isolation of a new MRV strain, GXLZ2301, from fecal samples. This strain replicates in multiple cell lines and forms lattice-like structures. Infected cells exhibit single-cell death and syncytia formation. The virus's titers peaked at 107.2 TCID50/0.1 mL in PK-15 and BHK cells, with the lowest at 103.88 TCID50/0.1 mL in A549 cells. Electron microscopy showed no envelope with a diameter of about 70 nm. Genetic analysis revealed GXLZ2301 as a recombinant strain with gene segments from humans, cows, and pigs, similar to type 3 MRV strains from Italy (2015-2016). Pathogenicity tests indicated that while the bovine MRV strain did not cause clinical symptoms in mice, it caused significant damage to the gut, lungs, liver, kidneys, and brain. The emergence of this MRV strain may pose a threat to the health of animals and humans, and it is recommended that its epidemiology and recombination be closely monitored.

Identification and Full-Length Sequence Analysis of a Novel Recombinant Goat Astrovirus Genotype in Guangxi, China.

Astroviruses are single-stranded, positive-sense RNA viruses capable of infecting humans as well as a wide range of mammalian and avian species, with a length of approximately 6.6-7.7 kb. In this study, 139 goat fecal samples collected from the Guangxi province were used for the RT-PCR detection, and two of these were positive for goat astrovirus, with a positivity rate of 1.44% (2/139). The complete genome sequence of an astrovirus strain and the partial genome sequence of a strain astrovirus, named GX WZ 2023 and GX HC 2023, were amplified and sequenced, and their sequence lengths were 6284 nt and 6213 nt, respectively. Among them, the capsid protein of goat astrovirus GX HC 2023 showed the highest amino acid identity of 95.9% with ovine astrovirus GX, which belonged to the MAstV-2 genotype. However, the closest relative of the GX WZ 2023 strain was found to be the caprine astrovirus Sichuan, with a nucleotide sequence identity of 76.8%. The ORF1ab nonstructural protein of this strain showed the highest amino acid identities of 89.2 and 95.8% with the ovine astrovirus S5.1 and caprine astrovirus G5.1 strains, respectively. However, its ORF2 capsid protein has 68.4% amino acid identity with the bovine astrovirus (BAstV) 16 2021 CHN strain and only 21.9-64% amino acid identity with all available strains of goat astrovirus. The GX WZ 2023 strain was recombined with the Chinese (BAstV 16 2021 CHN) and Japanese bovine strains (BAstV JPN 2015) in the ORF2 region. Therefore, the goat astrovirus GX WZ 2023 is proposed as a new member of the family goat astroviridae based on the species classification criteria of the International Committee on Taxonomy of Viruses. These findings enhance our understanding of the prevalence and genetic evolution of goat astrovirus and provide a scientific basis for future studies of these viruses in other animals.

Association between serum lactate dehydrogenase and lymph node metastasis in cervical cancer.

The aim of the present study was to evaluate the association between serum lactate dehydrogenase (LDH) and the risk of lymph node metastasis (LNM) in the International Federation of Gynecology and Obstetrics (FIGO) 2009 cervical cancer (CC) stages IB1-IIA2. All patient medical records with FIGO 2009 stage IB1-IIA2 CC between January 2012 and January 2022 were analyzed retrospectively. The association between serum LDH and LNM was assessed using uni- and multivariate logistic regression analyses, subgroup analyses and P-splines. The present study included 586 patients, 91 (15.5%) of whom had LNM. Patients with an elevated LDH level were more likely to have a deep stromal invasion, lymph-vascular space invasion, LNM and to be of an older age. Multivariate logistic regression revealed a significant association between LNM and LDH levels. After adjusting for age, FIGO stage, tumor markers and risk factors according to the Sedlis criteria, patients in the highest LDH quartile had an increased risk of LNM compared with those in the lowest LDH quartile (odds ratio, 3.5; 95% CI, 1.57-7.81). Furthermore, P-spline regression revealed a dependence of LNM on LDH. The predictive value of LDH level remained significant in the subgroup analysis. The present study suggested that a higher LDH level was independently associated with CC and LNM, and that LDH level may serve as a potential tumor marker and treatment-related indicator.

Non-linear effects of meteorological variables on cooling efficiency of African urban trees.

Urban tree cover is widely regarded as an environmentally-friendly and effective urban cooling approach. Meteorological variables, including air temperature, wind speed, and humidity, have complex impacts on the cooling efficiency (CE) of urban trees (i.e., the negative ratio of the land surface temperature change to the tree cover percentage change). This means that increasing the urban tree cover to alleviate heat stress is not necessarily suitable for cities with different climates. African cities are confronted with larger heat risks but lack considerations for the effectiveness of urban tree cooling in urban planning. In this study, 40 African major cities with population greater than 500,000 in different climatic regions were selected, and 1459 CEs during each city's corresponding warmest 3 consecutive months were calculated combined with the availability of meteorological data. The generalized additive models revealed the non-linear impacts of air temperature/temperature dew point difference on CE, which were more evident in arid cities. The CE of urban trees actually increased and then decreased along with the increase of air temperature/temperature dew point difference, and the turning point were 34 °C/26 °C, respectively. African cities would have different frequencies of warm days with an air temperature over 34 °C under different Shared Socioeconomic Pathways within the next 30 years. Specially, the cities around Sahel would suffer up to 40-60% days over 34 °C, which meant their urban tree CE would decrease along with air temperature increase. This study highlighted that in African cities, especially those with arid climate, it was unadvisable to only count on increasing tree cover to alleviate urban heat stress in the warming future, which called for other combined cooling strategies.

Modelling regional ecological security pattern and restoration priorities after long-term intensive open-pit coal mining.

Long-term intensive open-pit mining can have huge impacts on ecosystems and the services they provide, affecting the integrity of ecosystem structures, functions and process and thus the "ecological security" of a whole mining region. The indirect and direct impacts of mining are spatially and temporally complex and therefore ecological security patterns need to be considered. However, to date there has been little research focusing on ecological security patterns in mining regions. This study aims to model and map ecological security and restoration priorities in an intensive open-cut coal mining region accounting for spatio-temporal changes of multiple ecosystem services. Four ecosystem services including habitat quality, carbon sequestration, water yield, and sediment retention were assessed and mapped in 1990, 2000, 2010, and 2020. Next ecological security patterns and restoration priorities were modelled and characterized using the Self-Organizing Feature Map to identify ecological sources, and circuit theory in Linkage Mapper to characterize connectivity and flows. The results showed that habitat quality, carbon sequestration, and water yield services were most affected by vegetation cover changes due to mining exploitation, while sediment retention was influenced by multiple natural factors, especially topography. Ecological sources, corridors, pinch-points and barriers showed various changing trends due to mined and built-up land expansion over time. Ecological source area declined from 1355.08km2 in 2000 to 584.92 km2 in 2020, while the number of corridors increased from 17 in 2000 to 25 in 2010 and then decreased to 21 in 2020. Although restoration has been conducted on some mine sites, regional-scale restoration needs greater consideration. This study provides decision-makers and stakeholders with a method for assessing regional-scale ecological security and restoration in a holistic and systematic way moving beyond a single mine, which is critical for balancing ecological security protection with minerals production in intensive mining regions.

Landscape eco-environmental research on littoral zone in China.

Littoral zone is a special land/landscape type. As an important kind of land resource in support, the use of littoral zone is vital to eastern coastal areas in China. And the research on littoral zone relates to the key theory of landscape ecology. Based on the theory of landscape ecology, the littoral zone was divided into four types: mud flat, sand beach, bench, and biological flat. The distribution of each type in China is pointed out. As a typical open system, littoral zone has six landscape ecological characteristics: (1) high sensitivity to disturbance; (2) distinct edge effect; (3) spatial aggregation of natural resources; (4) frequently spatial oscillation; (5) obviously spatial heterogeneity; and (6) noticeably spatial differentiation. Some proposals are also put forward on the land use and development of littoral zone for environmental protection and environmental management.

[Landscape regionalization of agro-pastoral transitional zone using self-organizing feature maps (SOFM) network: a case study of Inner Mongolia Autonomous Region, China].

Taking the agro-pastoral transitional zone of Inner Mongolia Autonomous Region, which has the most extrusive landscape differentiation in Northern China, as the object, a landscape spatial differentiation index was established based on the NDVI, integrated index of land use degree, and aridity index, and the characteristics of the landscape spatial differentiation were quantitatively identified by using SOFM network. The study area could be divided into six landscape zones, i. e., forest zone, forest-steppe transitional zone, agro-steppe mixed zone, typical steppe zone, steppe-desert transitional zone, and desert zone. As compared with the traditional schemes of physical geographical regionalization, our landscape regionalization could quickly regionalize the large scale landscape spatial differentiation in a quantitative way, and the regionalization results were basically in accordance with the original landscape characteristics and the landscape spatial differentiation pattern of the study area.

Antinociceptive and anti-inflammatory activities of ethyl acetate fraction from Zanthoxylum armatum in mice.

Zanthoxylum armatum DC. is a traditional Chinese medicine that is prescribed to alleviate pain and treat inflammatory disorders. This species is distributed mainly in the southeast and southwest regions of China. In the present study, we found that ethyl acetate fraction of ethanolic extract of Z. armatum could significantly decrease acetic acid-induced writhing numbers, and suppress formalin induced licking times in the first phase at the highest dose and in the second phase at all tested doses. This observation revealed that Z. armatum extract possessed powerful antinociceptive activity. The mechanisms of the antinociceptive effect might be mainly involved in the periphery inflammatory analgesic. In addition, the ethyl acetate fraction also inhibited xylene-induced ear swelling in a dose-dependent manner in mice. Eight lignans [eudesmin, horsfieldin, fargesin, kobusin, sesamin, asarinin, planispine A, and pinoresinol-di-3,3-dimethylallyl] were identified as major components of the ethyl acetate fraction. Considering related studies reporting the anti-inflammatory activity for the identified lignans, lignan might be responsible for its anti-inflammatory activity. Our results confirm that the traditional use of Z. armatum in the treatment of inflammation and pain is warranted.

Applicability study of using in-situ gamma-ray spectrometry technique for 137Cs and 210Pb(ex) inventories measurement in grassland environments.

In-situ measurement of fallout radionuclides (137)Cs and (210)Pb(ex) has the potential to assess soil erosion and sedimentation rapidly. In this study, inventories of (137)Cs and (210)Pb(ex) in the soil of Inner Mongolia grassland were measured using an In-situ Object Counting System (ISOCS). The results from the field study indicate that in-situ gamma-ray spectrometry has the following advantages over traditional laboratory measurements: no extra time is required for sample collection, no reference inventories are required, more economic, prompt availability of the results, the ability to average radionuclide inventory over a large area, and high precision.

[Relationships between landscape pattern and water quality at western reservoir area in Shenzhen City].

In this paper, the relationships between landscape pattern and water quality at western reservoir area in Shenzhen City were studied with grey connection method, and the influences of source' and 'sink' landscape patterns on non-point pollution were probed. The results showed that the dominance, adjacency, and fragmentation of 'source' and 'sink' landscapes could markedly influence the water quality. From 2000 to 2001, due to the changes of the 'source' and 'sink' landscape patterns in research areas, the output of pollutants increased and the reduction of pollution decreased, resulting in the deterioration of water quality of three reservoirs. According to the spatial distribution of 'source' and 'sink' landscapes, it was found that the distribution of 'sink' landscapes at the middle-lower reaches of the watersheds had close relationships with the changes of reservoir water quality, suggesting that 'sink' landscape pattern was of significance in the management of non-point pollution.

Evaluation for sustainable land use in mountain areas of Northwestern Yunnan Province, China.

As an important component of sustainable development in mountain areas, evaluation for sustainable land use is always one of the hotpots of researches on sustainable development. Traditional evaluation for sustainable land use mainly focuses on the sustainability of land use model and biological production on temporal scale, and overlooks the effects of land use patterns on the sustainability, while landscape ecology can be a good help to realize the spatial analysis of sustainable land use. In this study, a synthetic evaluation indexes system for sustainable land use was constructed through the application of landscape metrics. Taking Yongsheng County of Yunnan Province, China as a case study, a series of quantitative evaluation were conducted in 1996, 1999 and 2001, to monitor the temporal dynamics of regional land use sustainability. Two indicators, contributing amount of indexes, and obstacle amount of indexes, were also set up to ascertain the significance of all the evaluation indexes to the evaluation results. The results showed that, in the study phases, the land use sustainability of the whole county had been low with a stable but great spatial difference, and great changes took place in regional land use system in 1999 with the deviation from the aim of sustainable land use. It also showed that, the most important indexes contributing for the land use sustainability in the study period, were the indexes of population density and land use degree, followed by the index of landscape diversity and cropping index. And the most important indexes counteracting the land use sustainability were the indexes of per unit area total production value of industry and agriculture, per unit area yield of cereal crops, landscape fragmentation, followed by the indexes of per unit area yield of economic crops and fertilizer consume per unit area.

Environmental impact assessment of industrial structure change in a rural region of China.

As the embodiment of human activities, the change of regional industrial structure is an essential driving factor of global environmental change. Consequently, the research on the change of regional industrial structure and associated effects on the environment is one of the key issues of researches on sustainable development, human-environment relationship, and regional response to global environment change. However, compared to the flourish of researches on environmental impact assessment of industrial departments, few studies have been conducted to assess the environmental impact of regional industrial structure. In this study, based on a synthetic analysis of environmental disturbances of different industrial departments, the environmental impact coefficient of industrial department associated with the index of environmental impact of industrial structure was constructed, so as to make a quantitative assessment of environmental impact of the change of regional industrial structure. And the results of the case study in Lijiang City, a rural region of China, have showed that there are two obvious changes of industrial structure in the study area from 1992 to 2003, associated with a continuous decreasing of the index of environmental impact of industrial structure, which indicated a positive environmental effects of the change of regional industrial structure.

[Research progress on urban green space].

Nature, diversity, and openness are what we demand from urban green space in the 21st century. Based on the summary of urban green space connotation, types and significance, this paper reviewed the research hotspots of urban green space, i.e., conservation and planning of urban-rural fringe, restoration and preservation of natural areas and indigenous vegetation, ecological restoration and planning of green way, biodiversity conservation, ecosystem structure and services, and management policy. The difference between foreign and domestic urban green space researches and practices were summarized, and some preferential urban green space research aspects in the future were proposed. It was suggested that in China, urban green space strategy should be integrated into urban planning and land use planning.

[Spatial-temporal dynamics of landscape fragmentation in North Shannxi Loess Plateau].

The study of landscape fragmentation is an important means to reveal regional ecological processes. By using the remote sensing data of 1987, 1997 and 2002, and the GIS tools, some representative quantitative indices of the landscape spatial structure and landscape fragmentation in North Shannxi Loess Plateau were calculated, aimed to investigate the relationships between landscape ecological structure and landscape pattern in this area. The results showed that cropland contributed most to the extent of landscape fragmentation, but unused land lacked obvious relationship with it. To get large-area images accurately and promptly is very important for monitoring ecological process in regional scale. Moderate-Resolution Imaging Spectroradiometer (MODIS) images in 2003 were used to derive monthly landscape fragmentation information calculated with Local Index of Spatial Associations (LISA) based on Spatial Statistical Analysis (SSA). The analysis of the fragmented landscape types showed that human disturbance had a main contribution to the seasonal landscape fragmentation. Furthermore, with the increasing percentages of cropland and unused land, landscape became more fragmented, while forestland coverage had inverse effects on the fragmentation.

[Landscape spatial changes in Shenzhen and their driving factors].

Based on GIS technology, the MSS image in 1978 and the TM images in 1986, 1990, 1995 and 1999 were used to study the landscape spatial changes in Shenzhen during the past two decades. The results showed that the regional landscape in Shenzhen changed dramatically, from a typical agricultural landscape to a rapidly urbanizing landscape. The percentage of cultivated land decreased from 31.06% to 2.67%, while that of built-up area increased from 0.33% to 22.52%. The landscape diversity index showed a tendency of increase in 1978-1995, followed by a decrease in 1999. The index of landscape contagion decreased firstly and then increased, and the landscape fragmentation index increased from 0.0042 to 0.0073. Modified fractal dimension index showed the same tendency as landscape contagion index. Human activity was the main driving factor for landscape change. The change of geometry shape of landscape patches was driven by the economic interests of individual economic cells, and that of spatial configuration of landscape components was associated with the allocation of industries. The diversity of the whole landscape was mainly associated with the natural and ecological conditions such as topography and landform, while its change was strongly affected by the whole level of social and economic development.