RESUMEN
In the majority of magnetic systems the surface is required to order at the same temperature as the bulk. In the present Letter, we report a distinct and unexpected surface magnetic phase transition at a lower temperature than the Néel temperature. Employing grazing incidence x-ray resonant magnetic scattering, we have observed the near-surface behavior of uranium dioxide. UO2 is a noncollinear, triple-q, antiferromagnet with the U ions on a face-centered cubic lattice. Theoretical investigations establish that at the surface the energy increase-due to the lost bonds-is reduced when the spins near the surface rotate, gradually losing their component normal to the surface. At the surface the lowest-energy spin configuration has a double-q (planar) structure. With increasing temperature, thermal fluctuations saturate the in-plane crystal field anisotropy at the surface, leading to soft excitations that have ferromagnetic XY character and are decoupled from the bulk. The structure factor of a finite two-dimensional XY model fits the experimental data well for several orders of magnitude of the scattered intensity. Our results support a distinct magnetic transition at the surface in the Kosterlitz-Thouless universality class.
RESUMEN
INTRODUCTION: Tm:YAG 2 mum cw laser prostatectomy was introduced in the treatment of benign ptostatic obstruction (BPO). Since then numerous studies have been published proving efficacy during follow-up. However, different surgical techniques were introduced with different names for similar techniques that complicate comparison. This reviews aim was to compare published data and break down surgical techniques to core points. The authors define validate appellations for different surgical techniques and propose further use of these names to ensure homogenous nomenclature. MATERIALS AND METHODS: All publications on Thulium:YAG prostatectomy have been included in this review. Articles were reviewed and associated due to the nature of the surgical approach. A systematic review of published data was performed. RESULTS: Sixteen peer-reviewed publications dealing with Tm:YAG laser prostatectomy were printed since 2005. Four different surgical principles are described, including vaporization, resection and enucleation. Follow-up, up to 24 months, showed durable functional results. CONCLUSION: Efficacy of Tm:YAG prostatectomy was shown. Surgical techniques include "Tm:YAG Vaporization of the prostate (ThuVAP)", "Tm:YAG VapoResection of the prostate (ThuVaRP)" and "Tm:YAG VapoEnucleation of the prostate (ThuVEP)". The almost blunt enucleation is introduced as "Tm:YAG laser enucleation of the prostate (ThuLEP)". The authors recommend the use of this neologism in the future. Further, large-scale prospective studies are needed to prove long-term durability. To initiate and canalize these upcoming studies, the Urothulium Study Group was founded, combining international experts on Thulium:YAG laser prostatectomy under its roof.
Asunto(s)
Terapia por Láser/instrumentación , Láseres de Estado Sólido , Prostatectomía/instrumentación , Hiperplasia Prostática/cirugía , Tulio , Humanos , Terapia por Láser/métodos , Masculino , Prostatectomía/métodosRESUMEN
Cnidocytes, the stinging cells of cnidarians, discharge nematocysts in response to physical contact accompanied by the stimulation of specific chemoreceptors. Cnidocytes in fishing tentacles of a sea anemone are now found to discharge nematocysts preferentially into targets vibrating at 30, 55, and 65 to 75 hertz. Moreover, in the presence of submicromolar concentrations of known chemosensitizers, such as N-acetylated sugars and mucin, these optima shift to 5, 15, 30, and 40 hertz, frequencies that correspond to the movements of swimming prey. Hence, chemoreceptors for these substances tune cnidocyte mechanoreceptors to frequencies that match the movements of the prey.
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Células Quimiorreceptoras/fisiología , Cnidarios/fisiología , Mecanorreceptores/fisiología , Anémonas de Mar/fisiología , AnimalesRESUMEN
BACKGROUND: The chaperonins, a family of molecular chaperones, are large oligomeric proteins that bind nonnative intermediates of protein folding. They couple the release and correct folding of their ligands to the binding and hydrolysis of ATP. Chaperonin 60 (cpn60) is a decatetramer (14-mer) of 60 kD subunits. Folding of some ligands also requires the cooperation of cpn10, a heptamer of 10 kD subunits. RESULTS: We have determined the three-dimensional arrangements of subunits in Rhodobacter sphaeroides cpn60 in the nucleotide-free and ATP-bound forms. Negative stain electron microscopy and tilt reconstruction show the cylindrical structure of the decatetramer comprising two rings of seven subunits. The decatetramer consists of two cages joined base-to-base without a continuous central channel. These cages appear to contain bound polypeptide with an asymmetric distribution between the two rings. The two major domains of each subunit are connected on the exterior of the cylinder by a narrower bridge of density that could be a hinge region. Binding of ATP to cpn60 causes a major rearrangement of the protein density, which is reversed upon the hydrolysis of the ATP. Cpn10 binds to only one end of the cpn60 structure and is visible as an additional layer of density forming a cap on one end of the cpn60 cylinder. CONCLUSIONS: The observed rearrangement is consistent with an inward 5-10 degrees rotation of subunits, pivoting about the subunit contacts between the two heptamers, and thus bringing cpn60 domains towards the position occupied by the bound polypeptide. This change could explain the stimulation of ATPase activity by ligands, and the effects of ATP on lowering the affinity of cpn60 for ligands and on triggering the release of folding polypeptides.
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Ventrículos Cardíacos/diagnóstico por imagen , Obesidad/diagnóstico por imagen , Disfunción Ventricular Izquierda/diagnóstico por imagen , Velocidad del Flujo Sanguíneo , Índice de Masa Corporal , Ecocardiografía , Ventrículos Cardíacos/patología , Humanos , Obesidad/patología , Volumen Sistólico , Disfunción Ventricular Izquierda/patología , Función Ventricular IzquierdaRESUMEN
Ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) catalyzes the key reaction of the Calvin reductive pentose phosphate cycle and as such is responsible for life as we know it. This enzyme has been intensively studied for decades. Evidence that RubisCO phylogenies are incongruent with those derived from other macromolecules has been accumulating and recent discoveries have driven home this point. Here we review findings regarding RubisCO phylogeny and discuss these in the context of the important biochemical and structural features of the enzyme. The implications for the engineering of improved RubisCO enzymes are considered.
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Ribulosa-Bifosfato Carboxilasa/genética , Ribulosa-Bifosfato Carboxilasa/metabolismo , Cianobacterias/enzimología , Cianobacterias/genética , Técnicas de Transferencia de Gen , Genes Bacterianos , Cinética , Modelos Moleculares , Estructura Molecular , Familia de Multigenes , Mutagénesis , Filogenia , Conformación Proteica , Ribulosa-Bifosfato Carboxilasa/químicaRESUMEN
A protein closely related to the Escherichia coli GroEL protein has been isolated from Rhodobacter sphaeroides. Native and SDS-polyacrylamide gel electrophoresis of this protein have shown that it is present in the cell as a multimeric complex of Mr 670,000 which is composed of a monomer of Mr 58,000. Antisera raised against the Mr 58,000 polypeptide have been shown to cross-react with GroEL and the alpha subunit of the pea plastid chaperonin. The N-terminal amino acid sequence of the Mr 58,000 polypeptide is identical to that of GroEL at 15 of 19 residues and is also closely related to the alpha subunit of the pea plastid chaperonin, though less so to the beta subunit.
Asunto(s)
Proteínas Bacterianas/análisis , Proteínas de Choque Térmico/análisis , Rhodobacter sphaeroides/análisis , Secuencia de Aminoácidos , Proteínas Bacterianas/genética , Proteínas Bacterianas/inmunología , Chaperonina 60 , Chaperoninas , Reacciones Cruzadas , Electroforesis en Gel de Poliacrilamida , Fabaceae/análisis , Proteínas de Choque Térmico/genética , Proteínas de Choque Térmico/inmunología , Datos de Secuencia Molecular , Peso Molecular , Plantas Medicinales , Proteínas/genética , Rhodobacter sphaeroides/genética , Rhodobacter sphaeroides/inmunología , Homología de Secuencia de Ácido NucleicoRESUMEN
An oligomer probe was designed to detect the presence of a putative phoB gene in the genome of the marine, phycoerythrin-containing cyanobacterium Synechococcus sp. WH7803. A 2.2 kb PstI fragment, identified using this probe, was cloned and the complete nucleotide sequence determined. The fragment contained two open reading frames encoding polypeptides which display all the sequence features expected of the response regulator and histidine protein kinase elements of a two component sensory system. Northern analysis confirmed that transcription of these genes was induced by phosphate limitation. On the basis of the sequence similarities and the regulation of their transcription by the availability of inorganic phosphate (Pi) these open reading frames were designated as phoB and phoR, respectively.
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Cianobacterias/genética , Cianobacterias/metabolismo , Genes Bacterianos , Fosfatos/metabolismo , Secuencia de Aminoácidos , Proteínas Bacterianas/genética , Secuencia de Bases , Clonación Molecular , ADN Bacteriano/genética , Datos de Secuencia Molecular , Sistemas de Lectura AbiertaRESUMEN
A young man with psychologic problems and a long history of social inadequacy presented with voiding dysfunction. Videocystometrography revealed a normal filling phase and normal initiation of voiding interrupted by considerable straining by the patient and marked sphincter electromyographic (EMG) activity. Temporary amelioration was achieved by infiltration of the sphincter with lignocaine hydrochloride and by biofeedback therapy. In such cases optimal results are expected from long-term behavioral therapy.
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Biorretroalimentación Psicológica , Retención Urinaria/psicología , Retención Urinaria/terapia , Adulto , Humanos , MasculinoRESUMEN
In response to damage to hair bundles caused by exposure to calcium free buffers, sea anemones secrete large protein complexes named 'repair proteins' that rapidly restore structural integrity and function to hair bundles. A specific chromatographic fraction of the repair protein mixture, named 'fraction beta', has biological activity comparable to the complete repair protein mixture (Watson et al., 1998, Hear. Res. 115, 119-128). In this study, we find that polyclonal antibodies raised against deglycosylated fraction beta specifically bind fraction beta on Western blots. Anti-fraction beta delays the normal recovery of vibration sensitivity in experimental animals (i.e., those with hair bundles damaged by calcium free buffers). Moreover, anti-fraction beta disrupts vibration sensitivity in control animals (i.e., those with healthy hair bundles). Experimentally damaged hair bundles subsequently exposed to repair protein and then processed for immunoelectron microscopy show labeled linkages interconnecting stereocilia of the hair bundle. Immunofluorescence microscopy confirms strong labeling of hair bundles treated with repair proteins and only weak labeling of tips of hair bundles from control animals. Immunofluorescence microscopy indicates stores of repair proteins in gland cells of the body column in control animals and in gland cells of the mouth in experimental animals. Repair biological activity is confirmed in column purified homogenates of these tissues. Apparently repair proteins are delivered to damaged hair bundles in mucus carried by beating cilia.
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Proteínas/inmunología , Proteínas/metabolismo , Anémonas de Mar/inmunología , Anémonas de Mar/metabolismo , Animales , Cilios/metabolismo , Cabello/metabolismo , Mecanorreceptores/metabolismo , Microscopía Fluorescente , Microscopía Inmunoelectrónica , Conejos , Anémonas de Mar/anatomía & histología , VibraciónRESUMEN
Blind cave fish use the lateral line sensory system to detect nearby objects. The fish responds to sudden perturbations in the water column by initiating startle responses in which they swim more rapidly. Normal startle responses disappear after trauma caused by a single 15 s immersion in calcium free water, but return within 5 days if the traumatized fish are treated with 'repair proteins' isolated from sea anemones. Polyclonal antibodies raised to fraction beta, a specific chromatographic fraction of repair proteins, bind to hair cells within superficial neuromasts. Likewise, biotinylated fraction beta binds to hair cells in neuromasts. Neuromast hair cells exposed to calcium free water followed by repair proteins have more compact hair bundles than do hair cells exposed only to calcium free water. We propose that anemone repair proteins replace linkages between stereocilia destroyed by exposure to calcium free water.
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Peces/fisiología , Mecanorreceptores/efectos de los fármacos , Mecanorreceptores/fisiología , Proteínas/aislamiento & purificación , Proteínas/farmacología , Anémonas de Mar/química , Animales , Avidina/metabolismo , Ceguera/fisiopatología , Células Ciliadas Auditivas/efectos de los fármacos , Células Ciliadas Auditivas/fisiología , Células Ciliadas Auditivas/ultraestructura , Inmunohistoquímica , Mecanorreceptores/ultraestructura , Microscopía Electrónica de Rastreo , Reflejo de Sobresalto/efectos de los fármacos , Reflejo de Sobresalto/fisiología , Regeneración/efectos de los fármacosRESUMEN
Sea anemones are among the simplest animals to use hair bundles to detect vibrations. Although we previously found anemone bundles to be morphologically similar to vertebrate hair bundles, only indirect evidence implicated anemone bundles in mechanotransduction. Here, we test mechanotransduction of these bundles using loose-patch current recording from apical membranes of cells at the base of deflected bundles. Step bundle deflection results in graded membrane currents that are inward in some cells (positive) and outward in other cells (negative). Positive responses range from 5 to 30 pA, abruptly saturate with stronger stimuli, and increase in duration with prolonged deflections. Negative responses range from 10 to 150 pA, show a logarithmic relation to stimulus strength, and attenuate with prolonged deflections. Additionally, responses are reversibly inhibited by streptomycin. We present a model for anemone bundle mechanotransduction modified from the gating spring model for vertebrate mechanotransduction. Because anemone bundles comprise stereocilia arising from a multicellular complex, we propose that supporting cells on opposite sides of a bundle function as oppositely polarized hair cells. Thus, deflection induces ion channels to open in cells on one side of the complex, while allowing channels to close in cells on the opposite side of the complex.
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Anémonas de Mar/anatomía & histología , Anémonas de Mar/fisiología , Adaptación Fisiológica , Animales , Fenómenos Biomecánicos , Polaridad Celular , Electrofisiología , Activación del Canal Iónico , Modelos Biológicos , Técnicas de Placa-Clamp , Estimulación Física , Anémonas de Mar/efectos de los fármacos , Estreptomicina/farmacología , Vertebrados , VibraciónRESUMEN
Sea anemones are marine invertebrates that use hair bundles to detect swimming movements of prey. Prey are captured by nematocysts (stinging capsules) that discharge into the prey. To further characterize anemone hair bundles and to compare hair bundles in anemones with hair bundles in vertebrates, we investigated fine structure and cytochemistry of anemone hair bundles. In addition, using a biological assay based on counting nematocysts discharged into vibrating test probes, we examined sensitivity of vibration detection to aminoglycoside antibiotics, Ca(2+)-free seawater, and amiloride. Like vertebrate hair bundles, anemone hair bundles are composed of stereocilia, possess lateral linkages between stereocilia whose preservation for transmission electron microscopy is enhanced by ruthenium red, and possess tip links morphologically similar to vertebrate tip links. Furthermore, vibration-dependent discharge of nematocysts is reversibly inhibited by 10(-4) M streptomycin and abolished by brief exposure to Ca(2+)-free seawater. However, unlike vertebrate hair bundles, anemone hair bundles appear to be insensitive to amiloride since vibration-dependent discharge of nematocysts is unaffected by up to mM amiloride. Thus, anemone hair bundles may serve as a useful model system for vertebrate hair bundles with the interesting feature of being insensitive to amiloride.
Asunto(s)
Células Ciliadas Auditivas/fisiología , Células Ciliadas Auditivas/ultraestructura , Mecanorreceptores/fisiología , Mecanorreceptores/ultraestructura , Anémonas de Mar/fisiología , Anémonas de Mar/ultraestructura , Amilorida/farmacología , Animales , Antibacterianos/farmacología , Calcio/farmacología , Cilios/ultraestructura , Células Ciliadas Auditivas/efectos de los fármacos , Mecanorreceptores/efectos de los fármacos , Microscopía Electrónica , Microscopía Electrónica de Rastreo , Modelos Biológicos , Anémonas de Mar/efectos de los fármacos , Agua de Mar , Especificidad de la Especie , Estreptomicina/farmacología , VertebradosRESUMEN
Sea anemones are sessile invertebrates that detect movements of prey using numerous hair bundles located on tentacles surrounding their mouth. Previously we found that hair bundles of anemones are structurally and functionally similar to those of vertebrates. After 10-15 min exposure to calcium depleted buffers, hair bundles in chickens suffer moderate damage from which they recover in 12 h without requiring new protein synthesis [Zhao, Yamoah and Gillespie, Proc. Natl. Acad. Sci. USA 94 (1996) 15469-15474]. We find that after 1 h exposure to calcium free seawater, hair bundles of anemones suffer extensive damage from which they recover in 4 h, apparently because of newly synthesized, secretory proteins called 'repair proteins'. Recovery is delayed in a dose dependent fashion by cycloheximide. In the presence of exogenously added repair proteins, recovery occurs within 8 min and is cycloheximide insensitive. Recovery is ascertained by a bioassay performed on intact specimens, by electrophysiology, and by timelapse video microscopy. Fraction beta, a chromatographic fraction with bioactivity comparable to the complete mixture of repair proteins, consists of complexes having an estimated mass of 2000 kDa. Avidin based cytochemistry suggests that biotinylated fraction beta binds to damaged hair bundles. SDS-PAGE gel electrophoresis demonstrates that fraction beta contains 8-10 polypeptides of 90 kDa or smaller. At least four of these polypeptides apparently are consumed during the repair process. Negatively stained samples of fraction beta are shown by transmission electron microscopy to include filamentous structures similar in length (150 nm) and width (6 nm) to linkages between stereocilia. The filamentous structures can be associated with globular structures (20 nm in diameter). A model is presented wherein repair proteins comprise replacement linkages and enzymes that attach linkages to appropriate membrane proteins.
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Células Ciliadas Auditivas/fisiología , Anémonas de Mar/fisiología , Animales , Calcio/análisis , Cicloheximida , Células Ciliadas Auditivas/ultraestructura , Histocitoquímica , Microscopía Electrónica , Peso Molecular , Biosíntesis de Proteínas , Inhibidores de la Síntesis de la Proteína , Agua de Mar/análisis , VibraciónRESUMEN
Hair bundle mechanoreceptors of sea anemones are similar to those of the acousticolateralis system of vertebrates (Watson, Mire and Hudson, 1997, Hear. Res. 107, 53-63). Anemone hair bundles are repaired by 'repair proteins' secreted following a complete loss of structural integrity and loss of function caused by 1 h exposure to calcium free seawater. Exogenously supplied repair proteins (RP) restore structural integrity to hair bundles and restore vibration sensitivity in 7-8 min (Watson, Mire and Hudson, 1998, Hear. Res. 115, 119-128). We here report that exogenously supplied ATP enhances the rate by which RP restore vibration sensitivity. A bimodal dose response to ATP indicates maximal enhancement at picomolar and micromolar concentrations of ATP. At these concentrations of ATP, vibration sensitivity is restored in 2 min. These data suggest that at least two ATPases exhibiting different binding affinities for ATP are involved in the repair process. Whereas the higher affinity site is specific for ATP, the lower affinity site does not discriminate between ATP and ADP. Nucleotidase cytochemistry localizes ATPase activity in isolated repair proteins. In the absence of exogenously added RP, sea anemones secrete and consume ATP during the 4 h recovery period after 1 h exposure to calcium free seawater. In the presence of exogenously added RP, ATP is secreted and then consumed within 10 min. Quinacrine cytochemistry localizes possible stores of ATP in the apical cytoplasm of sensory neurons located at the center of the hair bundle. According to our model, ATP is secreted by the sensory neuron after its hair bundle loses structural integrity. Hydrolysis of ATP by repair proteins is essential to the repair process.
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Adenosina Trifosfato/fisiología , Mecanorreceptores/fisiología , Anémonas de Mar/fisiología , Adenosina Trifosfato/metabolismo , Adenosina Trifosfato/farmacología , Animales , Espacio Extracelular/metabolismo , Histocitoquímica , Mecanorreceptores/efectos de los fármacos , Mecanorreceptores/ultraestructura , Microscopía Electrónica , Nucleotidasas , Proteínas/fisiología , Quinacrina , Anémonas de Mar/metabolismo , Agua de Mar , VibraciónRESUMEN
Benign prostatic hyperplasia (BPH) is a very common condition affecting over 800,000 American males each year. A standard, effective, and well-proven therapy is prostatectomy. This surgical procedure is used to treat, in the United States, approximately 400,000 BPH patients annually. Major treatment benefit is expected in 70% to 80% of patients. Complications are seen in 20% of the surgically treated patients. Due to the advanced age of BPH patients and the presence of other serious coexisting medical problems, surgical therapy may be difficult to utilize. These patients, who present a high risk for surgery, are in need of alternative treatments. Alternative therapy in BPH patients with clinically important symptoms and signs of urinary outflow obstruction include treatment with pharmacological agents, balloon dilatation, laser beam therapy, transurethral thermal therapy, transrectal microwave hyperthermia, and transurethral microwave hyperthermia. These alternative treatment modalities are currently under intensive study. These new treatment modalities ultimately must be compared with the standard treatment, which is prostatectomy. Due to the unpredictable natural history of BPH, it is desirable that each Phase III study should contain a no-treatment observation-only arm. Adenocarcinoma of the prostate (CaP) has become a tumor, which first in frequency, and second in importance in cancer mortality statistics of American males. Local tumor control rates and long-term survivals, with radical prostatectomy or radiation therapy, have been excellent. There was, however, recent concern regarding a high incidence of microscopic local tumor recurrence following a definitive course of irradiation. Deep regional or intracavitary hyperthermia (HT) with phase steering may be of value as an adjuvant treatment to radiotherapy. This HT may increase the incidence of local tumor control obtained with radiotherapy. Phase I-II clinical studies are currently underway.
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Hiperplasia Prostática/terapia , Neoplasias de la Próstata/terapia , Antagonistas Adrenérgicos alfa/uso terapéutico , Antagonistas de Andrógenos/uso terapéutico , Cateterismo/métodos , Ensayos Clínicos como Asunto , Diatermia/métodos , Humanos , Hipertermia Inducida/métodos , Terapia por Láser/métodos , Masculino , Microondas , Prostatectomía/efectos adversos , Prostatectomía/métodos , Prostatectomía/normas , Hiperplasia Prostática/clasificación , Hiperplasia Prostática/diagnóstico , Hiperplasia Prostática/fisiopatologíaRESUMEN
Recently great interest has been generated in alternatives to transurethral resection for benign prostatic hyperplasia. Lasers are currently being assessed, but marketing has for the moment outstripped basic science. A cystoscopic approach was used delivering Nd:YAG or KTP laser energy via forward and sidefiring fibers and contact tip devices in 51 patients. The sidefiring device is intended to coagulate a volume of prostate that subsequently sloughs, leaving a cavity. Treatment of the apical and middle lobe tissue using this technique was unsatisfactory. A further disadvantage was the interval between treatment and improvement in urine flow, which was approximately 6 weeks. The use of temporary prostatic stents has helped to overcome this delay in treatment effect. Encouraging early results have been achieved using forward-firing fibers to treat apical and middle lobe tissue. The use of contact tip devices to perform bloodless prostatotomies in combination with sidefire or bare fiber has also proved useful. Laser prostatectomy is an exciting field with considerable potential but remains in the developmental stage.
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Terapia por Láser , Prostatectomía/métodos , Anciano , Anciano de 80 o más Años , Estudios de Seguimiento , Humanos , Masculino , Persona de Mediana Edad , Hiperplasia Prostática/cirugía , Estudios RetrospectivosRESUMEN
Cnidocytes, the stinging cells of enidarians, discharge enidae (intracellular capsules containing eversible tubules) in response to physical contact combined with the stimulation of specific chemoreceptors. These receptors, occurring in at least two classes, bind N-acetylated sugars and certain amino-compounds, respectively (Thorington and Hessinger, 1988). Colloidal gold coated with bovine submaxillary mucin (mucin-gold) binds exclusively at the surface of the supporting cells which surround enidocytes (Watson and Hessinger, 1986). We now find that mucin-gold sensitizes enidocytes to discharge nematocysts in a dose-dependent manner. In addition, we find that the number of mucin-gold particles appearing at the surface of supporting cells changes over time, and that such changes correlate with the time-course of enidocyte responsiveness. Thus, the discharge of nematocysts by enidocytes may be regulated by the number of receptor-ligand complexes at the surface of the adjacent supporting cells. We conclude that enidocytes and supporting cells constitute receptor-effector complexes. Subsequent to binding at the cell surface, mucin-gold is endocytosed (Watson and Hessinger, 1987a). Multivesicular bodies seem to dispose of the endocytosed mucin-gold at the cell surface rather than via lysosomes. This novel route appears to be the major pathway by which endocytosed mucingold is removed from supporting cells.
RESUMEN
Collodial gold coated with the glycoprotein, bovine submaxillary mucin (BSM-gold), was used to localize chemoreceptors known to be involved in triggering the discharge of cnidae in sea anemones. BSM-gold binds exclusively at the apical surface of the supporting cell, the cell adjacent to the cnidocyte (Watson and Hessinger, 1986). Subsequent to binding, BSM-gold is internalized into endosomes and then translocated to multivesicular bodies (MVBs) and lysosomes. At cold temperature (4 degrees C), BSM-gold appears in endosomes near the surface of the cell but not in endosomes located more medially in the cell, nor in MVBs or lysosomes. The kinetics and sequence of intracellular translocation of BSM-gold were studied by fixing animals at various intervals following incubation in BSM-gold. Unlike that for supporting cells adjacent to non-cnidocytes, the amount of gold at the surface of supporting cells adjacent to penetrant cnidocytes does not seem to change despite considerable internalization of the mucin-probe. Apparently, free receptors replace receptor-ligand complexes in a one-for-one fashion in these cells.
RESUMEN
The mature nematocyst lies just beneath the cnidodyte plasma membrane. A microtubule array surrounds the nematocyst capsule just beneath the capsule tip. We propose that the array helps to hold the capsule at the cnidocyte cell surface until discharge. The undischarged capsule tip is sealed by three apical flaps, joined together along complex radial seams. The seams are filled with subunits that appear to bind the flaps together. Upon discharge, the flaps separate along the radial seams to permit thread eversion. The everted thread is lined on both sides by subunits that are stained by antimonate, indicating that they bind calcium. We suggest that, together, the subunits hold the uneverted thread in its folded and coiled configuration. Thread eversion would follow subunit uncoupling. The capsule and thread interiors of partially discharged nematocysts are stained by antimonate. In contrast, the capsule and thread interiors of fully discharged nematocysts are not stained by antimonate. Thus, nematocyst calcium might be injected into the target tissue where it is presumed to act in conjunction with nematocyst venom to promote cell death.