Asunto(s)
Infecciones por Citomegalovirus/sangre , Trasplante de Riñón/fisiología , Serina Endopeptidasas/sangre , Adulto , Infecciones por Citomegalovirus/inmunología , Citotoxicidad Inmunológica , Ensayo de Inmunoadsorción Enzimática , Femenino , Estudios de Seguimiento , Granzimas , Humanos , Trasplante de Riñón/inmunología , Estudios Longitudinales , Masculino , Persona de Mediana Edad , Complicaciones Posoperatorias , Estudios Retrospectivos , Factores de TiempoAsunto(s)
Linfocitos T CD8-positivos/inmunología , Infecciones por Citomegalovirus/inmunología , Infecciones por Herpesviridae/inmunología , Memoria Inmunológica , Trasplante de Riñón/inmunología , Selectina L/sangre , Complicaciones Posoperatorias , Serina Endopeptidasas/sangre , Adulto , Antígenos CD/sangre , Ciclosporina/uso terapéutico , Femenino , Granzimas , Antígenos HLA-DR/sangre , Herpesvirus Humano 4 , Humanos , Inmunosupresores/uso terapéutico , Trasplante de Riñón/fisiología , Masculino , Persona de Mediana Edad , Prednisolona/uso terapéutico , Receptores de Interleucina-2/sangre , Subgrupos de Linfocitos T/inmunología , Trasplante HomólogoRESUMEN
The physical maps of the DNAs of two cytomegalovirus isolates, AD169 and SG, were compared by cross-blot hybridization and by hybridization of nitrocellulose-bound SG fragments with cloned 32P-labelled AD 169 fragments. From this comparison it can be concluded that both physical maps are co-linear to a large extent. Most variability existed at the termini of the long and short component (at the repeats). Other differences were the presence or absence of restriction endonuclease cleavage sites.
Asunto(s)
Proteínas Bacterianas , Citomegalovirus/genética , ADN Viral/análisis , Desoxirribonucleasas de Localización Especificada Tipo II , Genes Virales , Secuencia de Bases , Enzimas de Restricción del ADN , Desoxirribonucleasa HindIII , Hibridación de Ácido NucleicoRESUMEN
BACKGROUND: T-lymphocytes that co-express CD4 and CD8 antigens may be found in small percentages in the peripheral blood of healthy individuals, and have a CD4brightCD8dull phenotype. CD4dullCD8bright T-lymphocytes have been found only in temporal association with some viral infections. METHODS: Four-colour flow cytometric analysis of peripheral blood mononuclear cells from a renal transplant recipient with cytomegalovirus infection was performed. RESULTS: A small but clearly distinguishable subpopulation of CD4dullCD8bright double-positive T-lymphocytes was detected, that exhibited phenotypic characteristics of cytotoxic T-lymphocytes and were granzyme B positive. Furthermore, no naive cells appeared to be present within this subpopulation. CONCLUSIONS: CD4dullCD8bright double-positive T-lymphocytes are enriched for memory and effector cytotoxic T cells.
Asunto(s)
Antígenos CD4/análisis , Antígenos CD8/análisis , Memoria Inmunológica , Serina Endopeptidasas/metabolismo , Linfocitos T/inmunología , Femenino , Granzimas , Humanos , Inmunofenotipificación , Persona de Mediana EdadRESUMEN
CD8+ T cells employ granzyme B (GrB) to induce apoptosis in target cells. Increased expression of GrB has been put forward as a diagnostic marker in transplant rejection and viral infection. Three-color flow cytometric analysis revealed that peripheral blood CD8+ T lymphocytosis during primary cytomegalovirus infection after renal transplantation resulted from expansion of a CD8+GrB+CD62L+ T cell subset that was almost absent during stable transplant function or acute rejection. This expansion coincided with a temporary increase in systemic soluble GrB (sGrB) levels. No such increase was observed during stable transplant function or acute rejection. Thus, the primary immune response to cytomegalovirus infection is accompanied by appearance of CD8+GrB+CD62L+ T cells and increased sGrB levels in the peripheral blood compartment. Determination of the latter may provide a novel approach for monitoring viral infections.