Asunto(s)
Antifúngicos/uso terapéutico , Trasplante Óseo , Micosis/terapia , Osteomielitis/terapia , Pseudallescheria , Pirimidinas/uso terapéutico , Triazoles/uso terapéutico , Adolescente , Antifúngicos/farmacología , Fracturas Abiertas/complicaciones , Humanos , Inmunocompetencia , Masculino , Pruebas de Sensibilidad Microbiana , Micosis/diagnóstico , Micosis/etiología , Osteomielitis/diagnóstico , Osteomielitis/etiología , Pseudallescheria/citología , Pseudallescheria/efectos de los fármacos , Pseudallescheria/aislamiento & purificación , Pirimidinas/farmacología , Fracturas de la Tibia/complicaciones , Trasplante Autólogo , Resultado del Tratamiento , Triazoles/farmacología , VoriconazolAsunto(s)
Infecciones por Campylobacter/epidemiología , Campylobacter coli/aislamiento & purificación , Campylobacter jejuni/aislamiento & purificación , Infección Hospitalaria/epidemiología , Brotes de Enfermedades , Animales , Austria/epidemiología , Infecciones por Campylobacter/microbiología , Campylobacter coli/clasificación , Campylobacter coli/genética , Campylobacter jejuni/clasificación , Campylobacter jejuni/genética , Pollos/microbiología , Niño , Infección Hospitalaria/microbiología , Contaminación de Alimentos , Personal de Salud , HumanosRESUMEN
The present study aimed at analysing the intensity of apoptosis, as related to the expression of pro- and anti-apoptotic cellular markers (cas-3, MT, Ki-67 antigen), and at evaluating their expression in relation to the survival time of patients with colorectal adenocarcinoma. Material for the studies was obtained from 40 patients with primary colorectal adenocarcinomas (G2, T3N0M0), treated at the Lower Silesia Centre of Oncology in Wroclaw. Tumour samples were fixed in 4% buffered formalin and embedded in paraffin blocks. In obtained paraffin sections, TUNEL reaction (detection of apoptosis) and immunocytochemical reactions were performed (detection of cas-3, MT and Ki-67 antigen expression). The results disclosed a weak correlation between the intensity of apoptosis and the expression of MT, cas-3 and Ki-67 antigen (r = 0.18; r = 0.33; r = 0.15, respectively) in cells of colorectal adenocarcinomas. The survival time of the patients was shorter, when the apoptosis and expression of Ki-67 antigen were highly pronounced. The time periods of the patients' survival showed no correlation with the expression of cas-3 or MT. The obtained results point to the key role of apoptosis and proliferation processes in the clinical course of colorectal adenocarcinomas.