Record-Breaking Rain Event Altered Estuarine Viral Assemblages.

Viruses are the dominant biological entity in the ocean, play a vital role in biogeochemical cycles, and provide their hosts with novel metabolic capabilities through auxiliary metabolic genes (AMGs). Hurricane Harvey was a category 4 hurricane that made landfall on the Texas coast in 2017 and lashed the Houston area with 1.4-1.7 × 1010 m3 of rainfall. In this paper, we aim to characterize how the changes in abiotic conditions brought by Hurricane Harvey altered the viral assemblages of Galveston Bay at the taxonomic level and determine how viral ecosystem functions were altered. Metagenomes of the viruses and their hosts were sequenced from a transect in Galveston Bay over the five weeks following the storm. Our results show that the viral assemblages of Galveston Bay dramatically changed following Hurricane Harvey's landfall. Of the abiotic parameters measured, salinity had the strongest effect on shaping the viral assemblages. In the five weeks following Hurricane Harvey, there was a steady increase of metabolic genes and putative viral infections. Our study provides the first in-depth look at how marine viral assemblages respond and recover from extreme rainfall events, which models predict will become more frequent and intense with climate change.

Functionally diverse microbial communities show resilience in response to a record-breaking rain event.

Estuaries provide many ecosystem services and host a majority of the world's population. Here, the response of microbial communities after a record-breaking flood event in a highly urbanized estuary was followed. Hurricane Harvey (hereafter Harvey) was a category 4 hurricane that made landfall on the Texas coast in 2017 and lashed the Houston area with 1.4-1.7 × 1010 m3 of rainfall, disrupting the natural gradients of nutrients and salinity. Here, we utilized metagenomics to analyze how Harvey altered the microbial community of Galveston Bay over five weeks following the storm. We hypothesized that the community would shift from a marine dominated community to that of a terrestrial and freshwater origin. We found that following the storm there were changes in the distribution of species with specific metabolic capacities, such as Cyanobacteria, enriched in oxygenic photosynthesis and nitrogen fixation genes, as well as Verrucomicrobia and Betaproteobacteria, with high prevalence of the SOX complex and anoxygenic photosynthesis genes. On the other hand, dominant members of the community with more diverse metabolic capabilities showed less fluctuations in their distribution. Our results highlight how massive precipitation disturbances can alter microbial communities and how the coalescence of diverse microorganisms creates a resilient community able to maintain ecosystem services even when the system is in an altered state.