Interaction between plant-based dietary pattern and frailty on cognitive decline: a longitudinal analysis of the Chinese Longitudinal Healthy Longevity Survey cohort.

OBJECTIVES: Frailty is a risk factor for faster cognitive decline, while plant-based dietary patterns are associated with decreased risk of cognitive decline. We aimed to explore their interaction with cognitive function among older adults. METHODS: We used data from the Chinese Longitudinal Healthy Longevity Survey between 2008 and 2018. Frailty was evaluated based on the frailty index (FI), and the plant-based diet index (PDI) was calculated using food frequency questionnaire at baseline. Repeated measures of the Mini-Mental State Examination (MMSE) were utilised to assess cognitive function. We used linear mixed models to estimate regression coefficients (ß) and 95% confidence intervals (CI). RESULTS: We included 7,166 participants with a median follow-up of 5.8 years. Participants in pre-frail (ß = -0.18, 95% CI: -0.24, -0.13) and frail (ß = -0.39, 95% CI: -0.48, -0.30) groups experienced an accelerated decline in MMSE score compared with the robust group. The PDI modified the above association, with corresponding associations with frailty being much more pronounced among participants with a lower PDI (frail vs. robust ß = -0.44, 95% CI: -0.56, -0.32), compared with those with a higher PDI (frail vs. robust ß = -0.27, 95% CI: -0.40, -0.13). In addition, A combination of frailty and a low PDI was strongly associated with a faster decline in MMSE score (ß = -0.52, 95% CI: -0.63, -0.41). CONCLUSION: Adherence to plant-based dietary patterns attenuates the association between frailty and cognitive decline. If the observed association is causal, promoting plant-based dietary patterns may be a strategy to reduce the effects of frailty on neurological health.

How did consumers' self-protective behavior formed during the COVID-19 pandemic?

Introduction: This study explored the formation mechanism of consumers' self-protective behavior during the COVID-19 pandemic, which is very important for policy settings to regulate consumer behavior. Based on the basic framework of the Protective Action Decision Model (PADM), this study analyzed the formation mechanism of consumers' self-protective willingness from the perspective of risk information, and explained the deviation between consumers' self-protective willingness and behavior from the perspective of protective behavior attributes. Methods: Based on 1,265 consumer survey data during the COVID-19 pandemic, the empirical test was carried out. Results and Discussion: The amount of risk information has a significant positive impact on the consumers' self-protective willingness, where the credibility of risk information plays a positive moderating role between them. Risk perception plays a positive mediating role between the amount of risk information and the consumers' self-protective willingness, and the positive mediating effect of risk perception is negatively moderated by the credibility of risk information. In the protective behavior attributes, hazard-related attributes play a positive moderating role between the consumers' self-protective willingness and behavior, while resource-related attributes play the opposite role. Consumers pay more attention to hazard-related attributes than resource-related attributes, and they are willing to consume more resources to reduce risk.

Environmental decentralization, environmental regulation, and green technology innovation: evidence based on China.

The reform of the environmental management system and policy optimization is key to promoting green technology innovation. However, empirical studies on environmental monitoring decentralization are limited. As a result, this paper analyzes the impacts of environmental decentralization and environmental regulation on green technology innovation using China's 30 provincial administrative panel data ranging from 2008 to 2017. The study outcome denotes that environmental decentralization is not conducive to green technology innovation. Similar effects are also found for environmental administration decentralization, environmental supervision decentralization, and environmental monitoring decentralization. Secondly, there is a U-shaped relationship between environmental regulation and green technology innovation. With the strengthening of environmental administrative decentralization and environmental supervision decentralization, environmental regulation has a significant positive role in promoting green technology innovation. Furthermore, environmental decentralization reduces the inhibitory effect of environmental regulation on green technology innovation in the eastern and central regions. Environmental decentralization does not play a regulatory role in the impact of environmental regulation on green technology innovation in economically developed areas. Lastly, the impact of environmental regulation on green technology innovation shows significant threshold characteristics with the change in environmental decentralization, in which there is an optimal threshold interval for environmental decentralization. The study concluded with an important reference value for determining reasonable levels of environmental decentralization among different regions and improving relevant environmental regulation strategies.

Associations of Leisure Activities and Sleep Duration with Cognitive Function: A Nationwide Prospective Cohort Study of Chinese Old Adults.

BACKGROUND: Leisure activities and sleep duration are correlated and have been linked to cognitive function, but most studies have examined only one of these factors. OBJECTIVE: To investigate the independent and joint associations of leisure activities and sleep duration with cognitive function among older adults. METHODS: We included 7,796 participants aged≥65 years from the Chinese Longitudinal Healthy Longevity Survey during 2008-2018 (waves 5-8). Self-reported leisure activities and sleep duration were assessed at baseline, and cognitive function was measured repeatedly using the Chinese version of the Mini-Mental State Examination (MMSE) at baseline and during follow-up. We used linear mixed models to estimate regression coefficients (ß) and 95% confidence intervals (CI). RESULTS: The median follow-up duration was 5.77 years. After adjusting for each other and potential confounders, both lower leisure activity score (each 1-point decrease ß= -0.33, 95% CI: -0.36 to -0.30) and longer sleep duration (each 1-hour increase ß= -0.17, 95% CI: -0.22 to -0.11) were independently associated with lower MMSE score. Furthermore, we observed an additive interaction between leisure activities and sleep duration (pinteraction < 0.001). A combination of low leisure activity score and long sleep duration was strongly associated with decreased MMSE score (ß= -2.51, 95% CI: -2.85 to -2.16) compared with the group with combined high leisure activity score and normal sleep duration. CONCLUSION: Both leisure activities and sleep duration were independently associated with cognitive function. Moreover, the combination of leisure inactivity and prolonged sleep duration predicted worse cognitive function (a preclinical hallmark of Alzheimer's disease) in an additive manner.

Insights into the trace Sr2+ impact on the gel properties and spatial structure of mutton myofibrillar proteins.

Myofibrillar proteins (MPs) and the quality of meat strongly depend on the properties of MP gels, which in turn depend on several parameters that include the thermal history and the concentration of metal ions. Strontium element (Sr) widely exists in mineral water and is found as strontium ions (Sr2+), which is an essential trace element for humans. This study investigated the effects of trace Sr2+ on the structure-function relationship of mutton MPs, as well as their gels with water. Trace concentrations of Sr2+ were found to significantly alter the conformation of the MPs. An increase in Sr2+ concentration was associated with a reduction in the tightness and strength of the gel and a significant increase in its water-holding capacity As compared to the untreated control sample, the solubility, particle size, and the magnitude of the Zeta potential of the gels increased by 13.03 %, 12.62 %, and 19.73 %, respectively, whereas the water retention capacity and the gel strength increased by 23.13 % and 21.90 %, at a Sr2+ concentration of 5.0 mg/L. Molecular docking predicted an increase in ionic bonds and disulfide bonds because Sr2+ had a strong interaction with hydrophilic amino acids and acidic amino acids. The analysis of molecular forces further verified the significant facilitation of interactions between MP molecules with the induction of Sr2+. As compare to the untreated control group, the ionic and disulfide bonds increased by 141.17 % and 66.94 %, when treated with 5.0 mg/L Sr2+. These changes were likely due to the enhancement of protein-protein interactions caused by Sr2+, which could induce MP molecules to properly unfold and aggregate in gel formation. The results could provide a basis for improving the texture and the quality of meat and meat products.

Effects of Citrus Fiber on the Gel Properties of Mutton Myofibrillar Protein.

This work investigated the effects of different additions of water-soluble citrus fiber (SCF) and water-insoluble citrus fiber (ICF) on the gel properties of the mutton myofibrillar protein (MP). The key parameters of water-holding capacity (WHC), rheological properties, and microstructure were evaluated. The addition of 2.5-10% of SCF and ICF significantly improved (p < 0.05) the WHC and gel strength of mutton MP gel. The rheological results showed that the viscoelasticity of MP with 5% SCF was the best, and the T2 relaxation time of the gel was significantly shortened. SEM results showed SCF reduced the number of pores in the MP gel, forming a more compact network structure. ICF stabilized the MP gel network structure as a filler after water absorption and expansion. However, the gel lost moisture under the action of strong external force (freeze-drying), which left large pores. These data confirmed that SCF and ICF could effectively improve the gel properties of meat products.

Ecological efficiency of hog scale production under environmental regulation in China: based on an optimal super efficiency SBM-Malmquist-Tobit model.

China's hog production is facing the dual pressures of the market and environment. A systematic analysis of the ecological efficiency (eco-efficiency) of hog cultivation is of great significance for the development of sustainability and distribution optimization in the industry. This paper investigates the eco-efficiency of hog production and the determinants of eco-efficiency in China using panel data (2004-2018). An optimal super efficiency slacks-based measure (SBM)-Malmquist-Tobit model is adopted for hog production analysis, and the empirical results show a great variation in eco-efficiency across provinces, ranging from 0.557 to 1.19 with a mean value of 0.937 in 2018. The predominant production area of hogs is found being transferred from north to south, with small- and medium-scale predominant production areas shifted from East China to Southwest China, and large-scale predominant production areas shifted from North China to South Central China. Another finding is that eco-efficiency increased by the improvement of technical efficiency. In addition, the Tobit regression results show that rural economic development, the government's investment in environmental control, the market advantage index, and transportation conditions had positive effects on the eco-efficiency; meanwhile, the forbidden policy for livestock cultivation in certain areas, the structure of the hog breeding industry, the density of slaughtered fattened hogs, and the prices of hogs had negative effects on the eco-efficiency.

Modulating the structure of lamb myofibrillar protein gel influenced by psyllium husk powder at different NaCl concentrations: Effect of intermolecular interactions.

In this study, a strategy involving psyllium husk powder (PHP) was proposed to alleviate the textural deterioration of protein gels under low-sodium conditions. Results revealed that myofibrillar protein (MP) in 0.3 M NaCl could accommodate more PHP to achieve better gels properties compared with that of 0.6 M NaCl. The 3 % addition of PHP could lessen the textural deterioration of gels at 0.3 M NaCl because of the insertion of PHP into the hydrophobic cavity of MP. Consequently, the reduction in protein viscoelasticity and the thermal stability of the head and tail of myosin improved. α-Helix structures unfolded, intermolecular forces formed, and proteins aggregated. Molecular docking predicted hydrogen bonds and hydrophobic interactions as the main forces to stabilize the conformation of composites. Experiments further verified that hydrophobic interactions and disulfide bonds were the main forces that stabilized the structure of MP-PHP composite gels.

Effect of different cooking water on flavor characteristics of mutton soup.

The mutton flavor is affected by cooking water significantly, and the flavor of mutton is delicious and widely loved by consumers through an extremely simple processing in northwest China, such as Inner Mongolia, Ningxia, and Xinjiang. The flavor shows obvious changes if get out of these areas even use the same raw meat, which may be caused by different cooking water. To determine whether and how the cooking water affect the mutton soup flavor, the elements in water, the flavor was studied by inductively coupled plasma mass spectrometry (ICP-MS), amino acid analyzer, and thermal desorption (TDS)-gas chromatography-mass spectrometry (GC-MS). Specifically, three water samples from different sources, Ningxia (NXW), Beijing (BJW), and ultrapure water from the laboratory (PUW), were used for cooking with Tan sheep's ribs to get different mutton soups. The inductively coupled plasma mass spectrometry (ICP-MS) results showed that the elements and the concentration of solutes in different water sources were significantly different. The NXW batch had the highest Na, Mg, K, and Sr concentrations, and Na in NXW water reached to 50.60 mg/L, which existed as Na+, significantly (p < .05) higher than BJW (8.63 mg/L) and PUW, which were important to the flavor of mutton soup. The PUW batch had the highest content of free amino acids, and the content of glutamic acid (Glu) reached to 17.89 µg/mL. The NXW batch had the highest content of taste nucleotides, and the content of 5´-IMP reached to 68.68 µg/ml. The volatiles of the three batches had significant differences, and only 40 volatiles were detected in all batches. Further flavor studies using electronic nose and electronic tongue showed significant differences in overall aroma and overall taste, especially bitterness, saltiness, and astringency. The results could provide a basis for improving the flavor quality for the mutton soup.

Osmotic stress alters circadian cytosolic Ca2+ oscillations and OSCA1 is required in circadian gated stress adaptation.

The circadian clock is a universal timing system that involved in plant physical responses to abiotic stresses. Moreover, OSCA1 is an osmosensor responsible for [Ca2+]i increases induced by osmotic stress in plants. However, there is little information on osmosensor involved osmotic stress-triggered circadian clock responses. Using an aequorin-based Ca2+ imaging assay, we found the gradient (0 mM, 200 mM, 500 mM) osmotic stress (induced by sorbitol) both altered the primary circadian parameter of WT and osca1 mutant. This means the plant switch to a fast day/night model to avoid energy consumption. In contrast, the period of WT and osca1 mutant became short since the sorbitol concentration increased from 0 mM to 500 mM. As the sorbitol concentration increased, the phase of the WT becomes more extensive compared with osca1 mutant, which means WT is more capable of coping with the environmental change. Moreover, the amplitude of WT also becomes broader than osca1 mutant, especially in high (500 mM) sorbitol concentration, indicate the WT shows more responses in high osmotic stress. In a word, the WT has much more flexibility to cope with the osmotic stress than osca1 mutant. It implies the OSCA1 might be involved in the circadian gated plant adaptation to the environmental osmotic stress, which opens an avenue to study Ca2+ processes with other circadian signaling pathways.

Phylogenetic and Molecular Evolutionary Analysis of Mitophagy Receptors under Hypoxic Conditions.

As animals evolved to use oxygen as the main strategy to produce ATP through the process of mitochondrial oxidative phosphorylation, the ability to adapt to fluctuating oxygen concentrations is a crucial component of evolutionary pressure. Three mitophagy receptors, FUNDC1, BNIP3 and NIX, induce the removal of dysfunctional mitochondria (mitophagy) under prolonged hypoxic conditions in mammalian cells, to maintain oxygen homeostasis and prevent cell death. However, the evolutionary origins and structure-function relationships of these receptors remain poorly understood. Here, we found that FUN14 domain-containing proteins are present in archaeal, bacterial and eukaryotic genomes, while the family of BNIP3 domain-containing proteins evolved from early animals. We investigated conservation patterns of the critical amino acid residues of the human mitophagy receptors. These residues are involved in receptor regulation, mainly through phosphorylation, and in interaction with LC3 on the phagophore. Whereas FUNDC1 may be able to bind to LC3 under the control of post-translational regulations during the early evolution of vertebrates, BINP3 and NIX had already gained the ability for LC3 binding in early invertebrates. Moreover, FUNDC1 and BNIP3 each lack a layer of phosphorylation regulation in fishes that is conserved in land vertebrates. Molecular evolutionary analysis revealed that BNIP3 and NIX, as the targets of oxygen sensing HIF-1α, showed higher rates of substitution in fishes than in mammals. Conversely, FUNDC1 and its regulator MARCH5 showed higher rates of substitution in mammals. Thus, we postulate that the structural traces of mitophagy receptors in land vertebrates and fishes may reflect the process of vertebrate transition from water onto land, during which the changes in atmospheric oxygen concentrations acted as a selection force in vertebrate evolution. In conclusion, our study, combined with previous experimental results, shows that hypoxia-induced mitophagy regulated by FUDNC1/MARCH5 might use a different mechanism from the HIF-1α-dependent mitophagy regulated by BNIP3/NIX.