[Determination of 16 elements in the different pine pollen by TXRF].

After microwave digestion, 16 elements in pine pollen were simultaneously determined by TXRF. The results show that all the 16 elements were found in all pine pollens. There was a significant difference in the average content of the element such as Ca, Ti, Mn, Zn and Rb between different groups of pine pollen (P < or = 0.01). There was a difference in the average content of the element such as K, V, Fe, Co, Cu and Sr between them (P < or = 0.05). And there was no difference in the average content of the element such as Cr, Ni, As, Pb and Se between them. The results also show that pine pollen has the spectral characteristics of warm property or cold property drug. They were closely related to the tree species and the growth environment or the growth area.

[Study on the interaction of methyl pheophorbide-a and bovine serum albumins by fluorescence].

The binding reaction of methyl pheophorbide-a with bovine serum albumins (BSA) in aqueous solution was studied by fluorescence and UV-Vis absorption spectra. The results indicated that the combination reaction of them was a single static quenching process. In aqueous solution, methyl pheophorbide-a strongly bound BSA with the apparent molar ratio of 2 : 1 because of methyl pheophorbide-a polymerized by itself. The binding constant KB was 6.7 x 10(4) L x mol(-1). In mixture solvent of tetrahydrofuran and water, methyl pheophorbide-a existed as single molecule and bound BSA with a molar ratio of 1: 1. There is single position for combining methyl pheophorbide-a with BSA. The shortest binding distance (r = 3.50 nm) and energy transfer efficiencies (E = 0.39) between donor (BSA) and acceptor (methyl pheophorbide-a) were obtained by Förster's nonradiative energy transfer mechanism.