RESUMEN
Cervical cancer is one of the most common gynecological malignant tumors. At present, it has been confirmed that the occurrence and development of cervical cancer is related to human papillomavirus infection. As a new regulatory molecule and research hotspot, circRNA is abnormally expressed in tumors and other diseases, and is expected to become a new biomarker for diagnosis and prediction of tumor occurrence and development. In this research, bioinformatics analysis and RT-PCR analysis showed that hsa_circ_0009143 (circRNA_PVT1) was up-regulated in cervical cancer. Knockdown of circRNA_PVT1 inhibits the migration and invasion of cervical cancer cells and would prevent pulmonary metastasis. Overexpression of circRNA_PVT1 induced migration and invasion of cervical cancer cells, which would result in the promotion of pulmonary metastasis. Finally, we found that circRNA_PVT1 can induce EMT of cervical cancer cells via targeting miR-1286 by exosome pathway, which can be a novel mechanism of cervical cancer progression.
Asunto(s)
Movimiento Celular , Transición Epitelial-Mesenquimal , Neoplasias Pulmonares/metabolismo , ARN Circular/metabolismo , Neoplasias del Cuello Uterino/metabolismo , Biología Computacional , Exosomas/genética , Exosomas/metabolismo , Femenino , Regulación Neoplásica de la Expresión Génica , Células HeLa , Humanos , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/secundario , MicroARNs/genética , MicroARNs/metabolismo , Invasividad Neoplásica , ARN Circular/genética , Reacción en Cadena en Tiempo Real de la Polimerasa , Transducción de Señal , Neoplasias del Cuello Uterino/genética , Neoplasias del Cuello Uterino/patologíaRESUMEN
OBJECTIVES: To analyze the prognosis and related risk factors of patients with scarred uterus complicated with central placenta previa. MATERIAL AND METHODS: A total of 272 parturient women admitted to our hospital between June 2013 and December 2016 were selected, of whom 142 cases with central placenta previa were designated as a control group and another 130 with scarred uterus complicated with central placenta previa were allocated as an observation group. The delivery outcomes of the two groups were compared, and the influencing factors were comprehensively analyzed. RESULTS: The prenatal and postpartum blood losses of the observation group were significantly higher than those of the control group (P < 0.05). The incidence rates of placental adhesion, placenta accreta, hysterectomy and puerperal infection in the obstetric group significantly exceeded those of the control group (P < 0.05). Logistic regression analysis showed that postpartum hemorrhage and placenta implantation were risk factors affecting prognosis (P < 0.05). CONCLUSIONS: Patients with scarred uterus and central placenta previa suffered from serious complications such as profuse postpartum hemorrhage and placental adhesion after delivery. Particular attention should be paid to women with scarred uterus during subsequent pregnancy to prevent placenta previa and to reduce the risks of delivery, thereby benefiting prognosis evaluation.