[Correlation analysis between prenatal exposure of per-/polyfluoroalkyl compounds and neonatal outcome].

Objective: To investigate the correlation between the prenatal exposure of per-/polyfluoroalkyl substances (PFASs) and the neonatal outcome. Methods: A total of 506 maternal infant cohort samples were collected in Hangzhou, Zhejiang province from 2020 to 2021. The exposure levels of seven PFASs in maternal serum before delivery were detected by solid-phase extraction-ultra performance liquid chromatography tandem mass spectrometry. Multivariable linear regression model was used to analyze the influence of prenatal exposure of PFASs on birth weight, birth length and Apgar score. Results: The maternal age, prenatal body mass index and gestation age were (31.3±4.3) years old, (26.7±3.2) kg/m2 and (265.0±28.3) days, respectively. The birth weight, birth length and scores of Apgar-1 and Apgar-5 were (3.1±0.8) kg, (49.3±2.9) cm, (9.88±0.47) points and (9.99±0.13) points, respectively. PFASs were widely distributed in maternal serum, with the highest concentration of (18.453±19.557) ng/ml, (6.756±9.379) ng/ml and (5.057±8.555) ng/ml for perfluorooctanoic acid (PFOA), perfluorooctane sulfonate (PFOS) and 6∶2 chlorinated polyfluorinated ether sulfonate (Cl-PFESA), respectively. Maternal age, parity and delivery mode were associated with the exposure level of PFASs (P<0.05). Subgroup analysis showed that PFOS had negative effects on birth weight (ß=-0.958), birth length (ß=-0.073) and Apgar-5 score (ß=-0.288) for neonates in the low birth weight (LBW) group. 6∶2 Cl-PFESA and 8∶2 Cl-PFESA inhibited the birth weight (ß=-0.926; ß=-0.552) and length (ß=-0.074; ß=-0.045) of newborn in the LBW group. In addition, 4∶2 fluorotelomer sulfonate (FTS) was associated with increased birth weight (ß=0.111) and decreased Apgar-5 score (ß=-0.030) in the normal weight group. Conclusion: Prenatal exposure to PFASs is associated with birth weight, birth length and Apgar-5 score. It is necessary to continue to pay attention to the impact of PFASs on fetal growth and development through maternal-fetal transmission.

A two-dimensional Sb/InS van der Waals heterostructure for electronic and optical related applications.

Stable configurations with excellent optical adsorption are crucial for photovoltaics or photocatalysis. Two-dimensional materials with intrinsic electric fields have been proposed as suitable for electric and optical devices. Here, we have performed DFT calculations on the electronic and optical properties of a bilayer Sb/InS van der Waals heterostructure, which consists of Sb and InS monolayers, by studying the band structures, charge density difference and distribution. Interestingly, the Sb/InS bilayer exhibits typical type-II band alignment character with a direct energy gap of 0.44 eV, and the electrons and holes are separated on different surfaces. Furthermore, applying an external E-field and biaxial strain is proved to be an effective way to modify the energy gap, the same as the electronic and optical properties. These theoretical predictions pave the way for high performance electronic and optical devices based on new two-dimensional van der Waals structures.

Limonitum Ameliorates Castor Oil-Induced Diarrhoea in Mice by Modulating Gut Microbiota.

Diarrhoea is a common clinical condition; its pathogenesis is strongly associated with gut microbiota dysbiosis. Limonitum is a well-known traditional Chinese medicine that exerts appreciable benefits regarding the amelioration of diarrhoea. However, the mechanism through which Limonitum ameliorates diarrhoea remains unclear. Here, the efficacy and underlying mechanism of Limonitum decoction (LD) regarding diarrhoea were explored from the aspect of gut microbiota. Castor oil (CO) was used to induce diarrhoea in mice, which were then used to evaluate the effects of LD regarding the timing of the first defecation, diarrhoea stool rate, degree of diarrhoea, diarrhoea score, intestinal propulsive rate, and weight of intestinal contents. The concentrations of short-chain fatty acids (SCFAs), including acetic, propionic, isobutyric, butyric and valeric acids, were analysed by gas chromatography-mass spectrometry (GC-MS). The 16S rRNA high-throughput sequencing technology was applied to evaluate changes in the gut microbiota under exposure to LD. LD was found to effectively ameliorate the symptoms of diarrhoea, and the diversity and relative abundance of gut microbiota were restored to normal levels following LD treatment. Additionally, LD significantly restored the observed reductions in SCFAs. These results provide strong evidence that LD can sufficiently ameliorate diarrhoea in mice by regulating their gut microbiota. The findings presented here highlight that Limonitum may constitute a prospective remedy for diarrhoea.

[Pathological features related to onco-immunity and their clinical significance of pancreatic ductal adenocarcinoma].

Objective: To investigate the tumor immunity-related pathologic features and clinical significance in pancreatic ductal adenocarcinoma (PDAC). Methods: All pathologic materials and clinical information of 192 PDAC patients from the Cancer Hospital of the University of Chinese Academy of Sciences from January 2010 to December 2020 were collected. The onco-immune microenvironment associated morphologic features were evaluated, and MHC-Ⅰ, PD-L1, CD3, and CD8 expression were detected by immunohistochemistry (IHC). Then the correlation between the factors and their influence on prognosis was analyzed. Results: There were 163 cases of non-specific adenocarcinoma (163/192, 84.90%), 18 cases of adeno-squamous carcinoma (18/192, 9.37%), and 11 cases of other rare subtypes (11/192, 5.73%). Perineural invasion was observed in 110 cases (110/192, 57.29%) and vascular invasion in 86 cases (86/192, 44.79%). There were 84 cases (84/182, 46.15%) with severe chronic inflammation. Tumor infiltrating immune cell numbers (TII-N) were increased in 52 cases (52/192, 27.08%). Lymphocytes and plasma cells were the main infiltrating immune cells in 60 cases (60/192, 31.25%), whereas in 34 cases (34/192, 17.71%) the tumors were mainly infiltrated by granulocytes, and 98 cases (98/192, 51.04%) showed mixed infiltration. CD3+T cells were deficient in 124 cases (124/192, 66.31%). CD8+T cells were deficient in 152 cases (152/192, 79.58%). MHC-Ⅰ expression was down-regulated in 156 cases (156/192, 81.25%), and PD-L1 was positive (CPS≥1) in 46 cases (46/192, 23.96%). Statistical analysis showed that TII-N was negatively correlated with vascular invasion (P=0.035), perineural invasion (P=0.002), stage (P=0.004) and long-term alcohol consumption (P=0.039). The type of immune cells correlated positively with chronic pancreatic inflammation (P=0.002), and negatively with tumor differentiation (P=0.024). CD8+T cells were positively correlated with CD3+T cells (P=0.032), MHC-Ⅰ expression (P<0.001) and PD-L1 expression (P=0.001), and negatively correlated with long-term smoking (P=0.016). Univariate analysis showed that histological nonspecific type (P=0.013) and TII-N (P<0.001) were the factors for good prognosis. Vascular invasion (P=0.032), perineural invasion (P=0.001), high stage (P=0.003) and long-term alcohol consumption (P=0.004) were adverse prognostic factors. COX multivariate risk analysis found that TII-N was an independent favorable factor for PDAC, while perineural invasion was an independent adverse risk factor. Conclusions: TII-N is an independent superior prognostic factor for PDAC, and significantly correlated with many factors; chronic alcohol consumption and smoking may inhibit onco-immunity in PDAC patients.

[Relationship between onco-immunological and morphologic characteristics of lymphoepithelioma-like carcinoma and lymphocyte subtypes of peripheral blood].

Objective: To study the relationship between the onco-immunological and morphologic characteristics of lymphoepithelioma-like carcinoma (LELC) and peripheral blood lymphocyte subtypes and its clinical significance. Methods: The pathologic and clinical data of 117 LELC patients who were admitted to the Tumor Hospital of the University of Chinese Academy of Sciences from 2006 to 2018 were collected. The histological classification was based on previously reported morphological classification method. The onco-immunological and morphologic characteristics of the tumors such as lymphoid follicle formation and interstitial fibrous hyperplasia, patient's peripheral blood lymphocyte subtypes and prognosis data were collected. The relationship between various factors and their impact on prognosis were analyzed. Results: There were 117 patients, including 61 females and 56 males. The male to female ratio was 0.9∶1.0. The age of onset was 24-89 years (median 52 years). Primary sites included head and neck (68 cases), lungs (26 cases), stomach (15 cases), and others (eight cases). Morphologically, 54 cases were type Ⅰ, 62 cases were type Ⅱ, and one case could not be classified. The onco-immunological and morphologic features of the LELC tumors showed a continuous spectrum. Interstitial TILs were noted from focally to diffuse, and the interstitial fibrous tissues were from hardly visible to obvious sclerotic. Formation of lymphoid follicles was seen in 42 patients; obvious fibrosis was seen in 31 cases. Data of peripheral blood lymphocyte subtyping by flow cytometry were available in 73 cases. These data included CD3+total T cells, CD3+CD4+helper T cells, CD3+CD8+cytotoxic T cells, CD3-CD56+natural killer (NK) cells, CD3-CD19+B cells, CD4+CD45RA-T helper induction subgroup, CD4+CD45RA+ T suppression induction subgroup, CD4+CD45RO+memory T cell subgroup, CD45RA+CD45RO+activated T cell subgroup, CD8+CD38+activated cytotoxic T cell, and CD25+lymphocytes and CD44+lymphocyte. The proportion of lymphocytes of each subtype was normal in most patients, but the proportion of CD44+lymphocytes in 61 cases (83.6%) was increased; the proportion of T cell suppression induced subgroups was decreased in 53 cases (72.6%). Correlation analysis found a significant correlation between clinical stage and NK cells (P=0.023); tumor histologic type and cytotoxic T cells were significantly positively correlated (P=0.012); while tumor cell morphologic differentiation was significantly related to total T cells (P=0.003) and NK cells (P=0.026); Formation of interstitial lymphoid follicles was positively correlated with memory T cell subsets (P=0.025); Tumor interstitial fibrosis was significantly positively correlated with T suppression-induced subpopulations (P=0.004), and was significantly negatively correlated with total T cells (P=0.023) and with the expression of CD44 adhesion molecules (P=0.003). Survival analysis found that lymphoid follicle formation was a favorable prognostic factor for LELC (P=0.001). Conclusions: The onco-immunological and morphologic features in LELC show a continuous spectrum; the tumor clinicopathological characteristics and onco-immunological morphology are closely related to peripheral blood T lymphocyte subtypes, and the formation of interstitial lymphoid follicles is a favorable prognostic factor for LELC.

[Association between Lipoprotein(a) and the characteristics of left main coronary artery plaque in patients with stable angina pectoris].

Objective: To investigate the relationship between Lipoprotein (LP) (a) level and the characteristics of tissue components of left main coronary artery (LMCA) plaque. Methods: A total of 102 patients with stable angina pectoris who underwent percutaneous coronary intervention (PCI) in the People's Hospital of Henan Province from June 2010 to October 2016 were included. We performed intravascular ultrasound-virtual histology (IVUS-VH) to their LMCAs and evaluated the tissue characteristics, and the blood level of total cholesterol (TC), triacylglycerol (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), ApoB, ApoA1, LP(a) were measured. According to the value of their LP(a) level they were divided into 2 groups (high LP(a) group (>300 mg/L) (n=35) and low LP(a) group (≤300 mg/L) (n=67)), then the relationship between the above lipid values and the tissue characteristics of the LMCA plaque in the patients were evaluated. Results: Patients with a high LP(a) had a larger percentage of fibrolipid volume and a smaller percentage fibrous volume compared to patients with a normal LP(a) (25%±5% vs 13%±6%, P<0.01 and 50%±8% vs 61%±9%, P<0.01). Using multivariate linear regression analysis after adjustment for the above-mentioned confounding factors, LP(a) had a significantly positive correlation with fibrolipid volume percentage (r=0.645, ß=0.29, P<0.01), and had a negative correlation with fibrous volume percentage (r=-0.467, ß=-0.32,P<0.01), suggesting that the LP(a) was associated with the vulnerability of the LMCA plaque. Conclusion: For the patients with stable angina pectoris, the LP(a) has a significantly positive correlation with the percentage of fibrolipid volume and a negative correlation with the percentage of fibrous volume, suggesting that the LP(a) could predict the vulnerability of the LMCA plaque.

[Effect of ambient fine particulate matters exposure on nasal oxidative stress level in patients with allergic rhinitis in Taiyuan city].

Objective: To explore the effect of ambient fine particulate matters (PM(2.5)) exposure on nasal oxidative stress level in patients with allergic rhinitis (AR). Methods: A panel of sixty AR patients was recruited as subjects. Four repeated measurements were carried out from June 2017 to January 2018. Nasal irrigation solution was collected and examined for malondialdehyde (MDA) and superoxide dismutase (SOD). Monitoring data of atmospheric pollutants and meteorological factors during measurement periods were also collected. Time activity pattern combined with micro-environment monitoring method was used to evaluate PM(2.5) exposure. Mixed effect model was applied to analyze the relationship between PM(2.5) exposure concentration and oxidative stress level. Results: 49 subjects accomplished the four repeated measurements, and the mean±SD of their age was (36.7±8.4) years old. The median of MDA and SOD in four measurement periods was 3.70, 3.70, 5.58, 6.24 nmol/ml, and 105.50, 102.50, 95.00, 96.50 U/ml. The concentration of PM(2.5) exposure in four measurement periods was (40.0±2.7), (41.5±2.5), (52.3±5.9) and (74.7±4.9) µg/m(3), respectively. Results of single pollutant mixed effect model analysis showed that 0-, 1-, 2-, 3-day lag concentrations of PM(2.5) was significantly positively associated with MDA, about ß (95%CI) estimated as 0.24 (0.17, 0.30), 0.34 (0.27, 0.41), 0.32 (0.20, 0.44) and 0.33 (0.23, 0.43), respectively. 0-, 1-, 2-day concentrations of PM(2.5) was significantly negatively associated with SOD, about ß (95%CI) estimated as -0.99 (-1.66, -0.31), -1.35 (-2.08, -0.62) and -0.94 (-1.80, -0.07), respectively. Multivariate analysis found that lag 1-day concentration of PM(2.5) was still significantly associated with MDA and SOD after controlling for temperature, age and other influencing factors. For a 10 µg/m(3) increase of PM(2.5) concentration, MDA increased 0.26 (95%CI: 0.18, 0.33) nmol/ml, and SOD decreased 0.87 (95%CI: 0.21, 1.53) U/ml. Conclusion: Our results suggested that PM(2.5) exposure can aggravate the nasal oxidative stress response of AR patients.

[Review of survey method for human time-activity pattern and its application in the exposure assessment of air pollutants].

Time activity pattern refers to the time and behavior of people at different locations. The knowledge of time-activity pattern is essential for air pollution exposure assessment when direct personal exposure monitoring can't not be conducted, because air pollutant concentrations may vary significantly by location and activity. This review is focused on the survey method for human time-activity pattern and its application in the exposure assessment of air pollutants. After comparing the diary-reported trips and Global Positioning System (GPS) recording method, we believed that diary-reported trips were more reliable in describing comprehensive and detail records of the behavior and environmental circumstances while GPS-recorded trend to maintain sound information of time, location and transportations. Meanwhile, according to different subjects, the air pollution exposure model could be divided for population and individuals. There were great difference between two types of model in many areas, including the required information about time, activity and microenvironment.

[Assessing the consistency of methods to measure travel behavior related to exposure to air pollution].

Objective: To compare diary-reported trips and Global Positioning System (GPS) recording trips and identify the reasons for the discrepancies between two methods. Methods: The survey was conducted in Jiangning district in Nanjing city during July to September and November to December, 2015. Both the diary records and GPS method were used to investigate the travel behavior of 33 retired adults for 5 consecutive days. The GPS traces were display in Google Earth and then split into trips to compare with the diary records according to time and location. χ(2) test was used to analyze the influence of trip characteristics on misreporting rates of each method. Results: A total of 1 087 trips in the survey can be compared between the diary (n=909) and the GPS method (n=912). 7.3% (79/1 087) of the trips were only recorded by GPS method, and 11.8% (128/1 087) were only reported in the diary. Of the remaining 880 trips recorded by the both methods, 86.7% (763/880) matched each other, while 13.3% (117/880) did not. For the matched trips, the difference between the trip durations recorded by diary and GPS method was 2.0 (quartile was 6.0) minutes and the diary method overestimated about 25.0% trip durations when compared with the GPS method. The accuracy rates were 84.8%(903/1 065) and 86.9%(925/1 065) for diary and GPS method, respectively. Both methods were more likely to misreport the trip under 5 minutes. The misreporting rates of diary method for trips under 5 minutes were 6.8 times higher than trips over 30 minutes (21.7% vs 3.2%). The reporting accuracy was also significantly different among trips by different travel mode (P<0.05) for both methods, diary method had the lowest accuracy in reporting vehicles recorders (69.3%, 133/192), while GPS method was more accuracy in both biking (91.9%, 136/148) and vehicles recorders(89.6%, 172/192). The main reasons for misreporting by diary method were forgetting or unwilling to record and failing to record trips according to travel mode, while forgetting to carry GPS device, bad GPS signal and failing to locate the position were the main reasons for misreporting by GPS method. Conclusion: The majority of the trips reported in diary and GPS method are well consistent with each other. Both of the methods should be used simultaneously in the survey of time-activity pattern to improve the data quality.

Visual detection technique for efficient screening and isolation of Salmonella based on a novel enrichment assay using chromatography membrane.

To detect Salmonella more efficiently and isolate strains more easily, a novel and simple detection method that uses an enrichment assay and two chromogenic reactions on a chromatography membrane was developed. Grade 3 chromatography paper is used as functionalized solid phase support (SPS), which contains specially optimized medium. One reaction for screening is based on the sulfate-reducing capacity of Salmonella. Hydrogen sulfide (H2S) generated by Salmonella reacts with ammonium ferric citrate to produce black colored ferrous sulfide. Another reaction is based on Salmonella C8 esterase that is unique for Enterobacteriaceae except Serratia and interacts with 4-methylumbelliferyl caprylate (MUCAP) to produce fluorescent umbelliferone, which is visible under ultraviolet light. A very low detection limit (10(1) CFU ml(-1)) for Salmonella was achieved on the background of 10(5) CFU ml(-1) Escherichia coli. More importantly, testing with more than 1,000 anal samples indicated that our method has a high positive detection rate and is relatively low cost, compared with the traditional culture-based method. It took only 1 day for the preliminary screening and 2 days to efficiently isolate the Salmonella cells, indicating that the new assay is specific, rapid, and simple for Salmonella detection. In contrast to the traditional culture-based method, this method can be easily used to screen and isolate targeted strains with the naked eye. The results of quantitative and comparative experiments showed that the visual detection technique is an efficient alternative method for the screening of Salmonella spp. in many applications of large-sized samples related to public health surveillance.

[Evaluating the electrophysiological features of tremor in Parkinson's disease and essential tremor using accelerometry].

Objective: To investigate the electrophysiological features of tremor intensity, frequency and frequency dispersion of Parkinsonian (PT group) and essential (ET group) tremors using accelerometry. Methods: The amplitude, frequency and frequency dispersion of rest tremor, postural tremor and the influence of weight on tremor of 35 PT patients and 40 ET patients in Department of Neurology, the Second Affiliated Hospital Zhejiang University School of Medicine from May 2015 to April 2016 were retrospectively analyzed and compared. Data of the more and less trembling hands were statistically elaborated. Results: In resting, postural and loading states, PT amplitudes of the less affected hand were smaller than the more affected one. The less affected hand were (147±32), (142±36), (157±40)µV, the more affected hand were (185±41), (164±29), (190±33)µV, respectively; ET amplitudes of the less affected hand were also smaller than the more affected one, and the less affected hand were (149±33), (157±33), (169±43)µV, the more affected hand were (176±39), (189±39), (213±36)µV, respectively; PT frequencies of the less affected hand were faster than the more affected one, with the less affected hand (6.1±1.1), (6.4±1.7), (6.5±2.0)Hz, the more affected hand (5.4±1.3), (5.5±1.1), (5.7±1.1)Hz, respectively, but ET frequencies of the less affected hand were similar to the more affected one, with the less affected hand (6.5±1.3), (7.0±1.2), (7.2±1.5)Hz, the more affected hand (7.0±1.0), (7.3±1.0), (7.6±1.1)Hz, respectively; in resting and postural states, PT frequency dispersions of the less affected hand were broader than the more affected one, the less affected hand were (2.1±0.6), (2.4±1.1)Hz, respectively; the more affected hand were (1.6±0.8), (1.7±0.9)Hz, respectively. But in loading state, PT frequency dispersions were similar in both sides (2.3±1.2, 2.2±1.1)Hz; In resting, postural and loading states, ET frequency dispersions were also similar in both two sides, the less affected hand were (2.0±1.1), (2.2±0.9), (1.9±1.0)Hz, the more affected hand were (1.8±0.6), (1.9±0.8), (2.1±0.7)Hz. Conclusions: Tremor intensity is significantly asymmetric both in PT and ET, while frequency and frequency dispersion are symmetric in ET but asymmetric in PT. Bilateral assessment of frequency related tremor parameters may be useful for differentiating ET from PT.

[Application of new chest holder in the median sternotomy for open heart surgery in adults].

Objective: To explore the application of new chest holder in the median sternotomy for open heart surgery in adults. Methods: Two hundred adult patients in Henan Provincial People's Hospital from May 2013 to May 2015 were enrolled in the study and randomly divided into two groups. Experimental group included 100 cases who accepted the new type of chest holder in the open heart surgery. Control group were also composed of 100 cases whose sternums were fixed with the pure steel wire cerclage. Sternal closure time was recorded since the sternum closing. All subjects were followed up to obtain the chest incision healing, the incidence of sternal dehiscence, infection and secondary thoracotomy, and thus to estimated the effect of new chest holder. Results: All patients in the experimental group reached the effect of firm closed chest surgery. Sternal closure time of experimental group was much shorter than that of control group[(10±2) vs (21±4) min, P<0.001]. No sternal instability, dehiscence, infection and secondary thoracotomy were found during the follow-up. However, in the control group, 3 secondary thoracotomy cases, 6 sternal dehiscence cases and 2 sternal infection cases were detected. Conclusions: New type of chest holder in the median sternotomy for open heart surgery is more convenient with small surgical trauma. It can also effectively reduce the incidence of sternal instability, dehiscence, infection and secondary thoracotomy.

Measurement of the hyperfine quenching rate of the clock transition in 171YB.

We report the first experimental determination of the hyperfine quenching rate of the 6s(2) (1)S(0)(F = 1/2) - 6s6p (3)P(0)(F = 1/2) transition in (171)Yb with nuclear spin I = 1/2. This rate determines the natural linewidth and the Rabi frequency of the clock transition of a Yb optical frequency standard. Our technique involves spectrally resolved fluorescence decay measurements of the lowest lying (3)P(0,1) levels of neutral Yb atoms embedded in a solid Ne matrix. The solid Ne provides a simple way to trap a large number of atoms as well as an efficient mechanism for populating (3)P(0). The decay rates in solid Ne are modified by medium effects including the index-of-refraction dependence. We find the (3)P(0) hyperfine quenching rate to be (4.42 ± 0.35) × 10(-2) s(-1) for free (171)Yb, which agrees with recent ab initio calculations.

[A multi-dimensional analysis of pollen broadcasting concerns in Chinese population: a large-scale multi-center cross-sectional survey].

Objective: To investigate the concern about pollen broadcasting in Chinese population from multiple dimensions and to understand the information about allergic rhinitis (AR) in China by analyzing related factors. Methods: From March 1 to September 30, 2022, a large-scale multi-center cross-sectional survey was conducted based on the Questionnaire Star platform in 21 Chinese hospitals. A total of 7 056 subjects from 7 regions in China: Northeast, North, East, Central, South, Southwest, and Northwest China were included. Basic characteristics (including social demographic characteristics and disease characteristics of AR patients), concern about pollen broadcasting, the willingness of pollen-induced AR (PiAR) patients to receive pollen broadcasting, and the treatment satisfaction rate of AR patients were collected. The chi-square test, multivariate linear regression model, and Logistic regression analysis were used to analyze the concern about pollen broadcasting in the Chinese population and related factors from multiple dimensions. Results: Among 7 056 subjects, 23.02% were concerned about pollen broadcasting. Among 3 176 self-reported AR and 1 019 PiAR patients, 25.60% and 39.16% were concerned about pollen broadcasting, respectively, which was higher than that of non-AR or non-PiAR subjects (χ2 value was 21.74 and 175.11, respectively, both P<0.001). Among AR patients, the proportion of spring and autumn allergen-positive patients concerned about pollen broadcasting was higher than that in perennial allergen-positive patients (χ2 value was 20.90 and 19.51, respectively, both P<0.001). The proportion of AR patients with asthma, sinusitis, allergic conjunctivitis, and cardiovascular and cerebrovascular diseases was higher than those without complications (χ2 value was 50.83, 21.97, 56.78, 7.62, respectively, all P<0.05). The proportion of AR patients in North China who could find pollen broadcasting locally was 31.01%, significantly higher than those in other regions (all P<0.05). Multivariate linear regression model analysis showed that among PiAR patients, those with higher per capita household income and higher AR disease cognition levels had been concerned about pollen broadcasting in the past, and those complicated with allergic conjunctivitis had stronger intention to receive pollen broadcasting (B value was 0.24, 0.13, 0.66, 0.47, respectively, all P<0.05). The higher the disease cognition level of PiAR patients, the stronger their willingness to actively participate in treatment (R2=0.72, P<0.001). Only 18.89% of AR patients felt satisfied with the treatment effect. Logistic regression analysis showed that in AR patients, the treatment satisfaction rate was significantly higher among those concerned about pollen broadcasting compared to those who were not (OR=1.83, P<0.001). Conclusions: Currently, the dissemination of pollen broadcasting in China is hindered by various factors such as disease cognition level. The treatment satisfaction among AR patients remains unsatisfactory.

[The rise and fall of the leprosy clinic in Ru Gao in the North of Jiangsu in the period of the Republic of China].

The North of Jiangsu was a traditional area for leprosy in Chinese history. In the period of the Republic of China, one of the largest leprosy clinics in China was established in Ru Gao (Jugao) with the help of Chinese and foreign charitable organizations, the Chinese central government and some squires. It was the only large clinic for leprosy in the North of Jiangsu. It was officially opened in 1924, attached to the Presbyterian Church hospital, and was closed in 1927 after the breakout of the civil war. It reopened in May 1933, hosted by a leprologist, Lee S. Huizenga. Ru Gao (Jugao) leprosy clinic treated around 1,000 lepers in the North of Jiangsu with medicines and language communication. The establishment of the leprosy clinic made a historical contribution in terms of preventing leprosy from spreading to Shanghai. It closed in the spring of 1938 because of the invasion of the Japanese military.