Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 33
Filtrar
Más filtros

Bases de datos
País/Región como asunto
Tipo del documento
Intervalo de año de publicación
1.
Gynecol Endocrinol ; 38(5): 443-447, 2022 May.
Artículo en Inglés | MEDLINE | ID: mdl-35244509

RESUMEN

BACKGROUND: Biochemical pregnancy is a type of embryo transfer failure, patients with unexplained repeated implantation failure (RIF) also have higher biochemical pregnancy rate. Our study intends to evaluate the effect of granulocyte colony-stimulating factor (G-CSF) in patients with unexplained RIF with low hCG levels in early pregnancy. METHODS: Unexplained RIF patients with low hCG levels after embryo transfer were allocated. G-CSF were administrated from the ninth days after embryo transfer. Clinical pregnancy, miscarriage and live birth rates were evaluated. RESULTS: The clinical pregnancy and live birth rates were 52.5% and 30%. CONCLUSION: G-CSF is an effective treatment for potential biochemical pregnancy in unexplained RIF patients.


Asunto(s)
Implantación del Embrión , Transferencia de Embrión , Tasa de Natalidad , Femenino , Factor Estimulante de Colonias de Granulocitos/farmacología , Factor Estimulante de Colonias de Granulocitos/uso terapéutico , Humanos , Embarazo , Índice de Embarazo
2.
Nano Lett ; 20(5): 3864-3871, 2020 May 13.
Artículo en Inglés | MEDLINE | ID: mdl-32353241

RESUMEN

The commonly employed formamidinium (FA)-containing perovskite solar cells (PSCs) exhibit a severe phase instability problem, thereby limiting their commercial applications. Here, both phase stability and energy efficiency of FA-based PSCs were improved by treating the perovskite surface with pyrrolidinium hydroiodide (PyI) salts, resulting in a 1D perovskite structure (PyPbI3), stacked on the original 3D perovskite. By employing in situ XRD measurements, we revealed that the temperature-dependent phase transition activation barrier was enhanced after forming the 1D/3D structure, resulting in a prolonged transition time by 30-40-fold. From the first-principle calculations, we found the thermodynamic energy difference between two phases reduced from -0.16 to -0.04 eV after the stacking of 1D PyPbI3, offering additional lifetime improvement. Moreover, the champion 1D/3D bilayer PSC exhibits a boosted power conversion efficiency of 19.62%, versus 18.21% of the control. Such 1D/3D bilayer structure may be employed in PSCs to enhance their phase stability and photovoltaic performance.

3.
Anal Chem ; 92(15): 10777-10782, 2020 08 04.
Artículo en Inglés | MEDLINE | ID: mdl-32649181

RESUMEN

The saturation of nonenzymatic blood glucose sensors at lower than normal blood glucose levels has blocked their practical applications. The mechanistic understanding of the saturation, however, has long been under debate. Employing cyclic voltammetry, amperometry, and FTIR with various electrolytes of varying concentrations, we were able to uproot the saturation cause. It was found to be related to the hydroxide ion concentration, which must be 11 times greater than that of the glucose concentration, contrary to the prior understanding. Together with the satisfactory sensitivity at high pH, nonenzymatic blood glucose sensing has finally been achieved, eliminating the usual problem of electrochemical current saturation as well as the need for enzyme found in the present technology.


Asunto(s)
Análisis Químico de la Sangre/métodos , Glucemia/análisis , Análisis Químico de la Sangre/instrumentación , Electroquímica , Electrodos , Concentración de Iones de Hidrógeno , Límite de Detección , Espectroscopía Infrarroja por Transformada de Fourier
4.
J Assist Reprod Genet ; 37(8): 1931-1938, 2020 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-32519010

RESUMEN

PURPOSE: Higher serum estradiol levels occur in women undergoing assisted reproductive technology (ART) owing to ovarian stimulation. Here, we investigated the association between maternal serum estradiol levels and the intellectual development of offspring conceived with ART. METHODS: A total of 204 singletons born after fresh embryo transfer were recruited for this cohort study. Among them, 102 children were born from mothers with high serum estradiol levels (> 12,000 pmol/L) on the day that human chorionic gonadotropin was administered. Another 102 children, matched by gestational age and age of the children, were recruited as controls from mothers with low serum estradiol (≤ 12,000 pmol/L). The Wechsler Preschool and Primary Scale of Intelligence was used to evaluate the intellectual development of the children. RESULTS: Children from mothers with higher serum estradiol levels scored lower in the verbal intelligence quotient (IQ) tests and verbal comprehension than children whose mothers had lower estradiol levels. The main difference between the two groups was in verbal subtests including information, vocabulary, and sorting. Partial correlation analysis revealed that the logarithm of maternal serum estradiol level negatively correlated with verbal IQ, performance IQ, and full scale IQ. CONCLUSION: Our data demonstrate that a high maternal serum estradiol level may negatively associate the verbal ability of children conceived via ART.


Asunto(s)
Estradiol/sangre , Discapacidad Intelectual/sangre , Inteligencia/fisiología , Técnicas Reproductivas Asistidas/efectos adversos , Adulto , Niño , Preescolar , Gonadotropina Coriónica/administración & dosificación , Estudios de Cohortes , Transferencia de Embrión/efectos adversos , Femenino , Fertilización In Vitro/efectos adversos , Humanos , Discapacidad Intelectual/etiología , Discapacidad Intelectual/fisiopatología , Pruebas de Inteligencia , Masculino , Inyecciones de Esperma Intracitoplasmáticas/efectos adversos
5.
Gynecol Endocrinol ; 31(4): 332-6, 2015 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-25558892

RESUMEN

OBJECTIVES: To evaluate different oral contraceptive pill (OCP) pretreatment associated differential in-vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) outcomes of polycystic ovary syndrome (PCOS) patients and explore enhanced hormonal balance induced by the pretreatment. METHODS: This retrospective study included 500 PCOS women and 565 normal ovulating counterparts undergoing IVF/ICSI. The PCOS patients were divided into three groups based on the OCP pretreatment regimens: non-OCP (without OCP pretreatment), unsuccessive OCP (the period of successive pretreatment ≤2 months) and successive OCP (the period of successive pretreatment ≥3 months) groups. Comprehensive hormonal and ultra-sonographic assessments were performed before/after IVF pretreatment. Confounding factors affecting pregnancy outcomes were analyzed with logistic regression. RESULTS: PCOS patients with significant endocrine disorders had reduced implantation and pregnancy rates and increased miscarriage rate. Successive, not unsuccessive OCP pretreatment, significantly improved the implantation and pregnancy rates, and reduced the incidence of monotocous small-for-gestational age infants, which was accompanied by remarkably decreased hyperandrogenism and antral follicles. CONCLUSION: PCOS is an independent risk factor for poor IVF outcome. Successive, not unsuccessive, OCP cyclical pretreatment could improve pregnancy outcome of PCOS patients, associated with reduction of hyperandrogenism and antral follicle excess.


Asunto(s)
Anticonceptivos Secuenciales Orales/uso terapéutico , Fertilización In Vitro , Hiperandrogenismo/prevención & control , Infertilidad Femenina/terapia , Síndrome de Hiperestimulación Ovárica/prevención & control , Inducción de la Ovulación/efectos adversos , Síndrome del Ovario Poliquístico/tratamiento farmacológico , Adulto , China/epidemiología , Femenino , Retardo del Crecimiento Fetal/etiología , Retardo del Crecimiento Fetal/prevención & control , Hospitales Universitarios , Humanos , Hiperandrogenismo/etiología , Infertilidad Femenina/etiología , Servicio Ambulatorio en Hospital , Síndrome de Hiperestimulación Ovárica/etiología , Síndrome del Ovario Poliquístico/fisiopatología , Embarazo , Índice de Embarazo , Estudios Retrospectivos , Inyecciones de Esperma Intracitoplasmáticas , Estadística como Asunto , Adulto Joven
6.
BMC Med ; 12: 240, 2014 Dec 16.
Artículo en Inglés | MEDLINE | ID: mdl-25511686

RESUMEN

BACKGROUND: The increasing number of babies conceived by in vitro fertilization and embryo transfer (IVF-ET) shifts concern from pregnancy outcomes to long-time health of offspring. Maternal high estradiol (E2) is a major characteristic of IVF-ET and lasts throughout the first trimester of pregnancy. The fetal thyroid develops during this period and may thus be affected by exposure to the supra-physiological E2. The aim of this study is to investigate whether the high E2 maternal environment in the first trimester increases the risk of thyroid dysfunction in children born following IVF-ET. METHODS: A cross-sectional survey design was used to carry out face-to-face interviews with consecutive children attending the hospital. A total of 949 singletons born after fresh embryo transfer (ET) (n=357), frozen ET (n=212), and natural conception (NC) (n=380), aged 3 to 10 years old, were included. All children were thoroughly examined. Meanwhile, another 183 newborns, including 55 fresh ET, 48 frozen ET, and 80 NC were studied. Levels of serum T3, FT3, T4, FT4, and TSH and levels of maternal E2 at different stages of the first trimester were examined. RESULTS: The mean serum E2 levels of women undergoing fresh ET during the first trimester of pregnancy were significantly higher than those of the women undergoing frozen ET or following NC. The thyroid hormone profile, especially the levels of T4, FT4, and TSH, were significantly increased in 3- to 10-year-old children conceived by fresh ET compared to NC. The same tendency was confirmed in newborns. However, levels of T4 and TSH in the frozen ET group were nearer to that of the NC group. Furthermore, levels of T4 and FT4 in fresh ET were positively correlated with maternal serum levels of E2 during early pregnancy. CONCLUSIONS: The maternal high E2 environment in the first trimester is correlated with increased risk of thyroid dysfunction. Frozen ET could reduce risks of thyroid damage in children conceived by IVF. Further studies are needed to confirm these findings and to better determine the underlying molecular mechanisms and clinical significance. TRIAL REGISTRATION: ChicCTR-OCC-14004682 (22-05-2014).


Asunto(s)
Transferencia de Embrión , Estradiol/efectos adversos , Fertilización In Vitro , Enfermedades del Recién Nacido/sangre , Exposición Materna/efectos adversos , Hormonas Tiroideas/sangre , Adulto , Niño , Preescolar , Estudios Transversales , Femenino , Humanos , Recién Nacido , Enfermedades del Recién Nacido/etiología , Masculino , Embarazo , Resultado del Embarazo , Primer Trimestre del Embarazo
7.
Sci Rep ; 13(1): 3449, 2023 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-36859466

RESUMEN

Projection imaging has been employed widely in many areas, such as x-ray radiography, due to its penetration power and ballistic geometry of their paths. However, its resolution limit remains a major challenge, caused by the conflict of source intensity and source size associated with image blurriness. A simple yet robust scheme has been proposed here to solve the problem. An unconventional square aperture, rather than the usual circular beam, is constructed, which allows for the straightforward deciphering of a blurred spot, to unravel hundreds originally hidden pixels. With numerical verification and experimental demonstration, our proposal is expected to benefit multiple disciplines, not limited to x-ray imaging.

8.
Materials (Basel) ; 16(5)2023 Feb 25.
Artículo en Inglés | MEDLINE | ID: mdl-36903040

RESUMEN

Bulk aluminum nitride (AlN) crystals with different polarities were grown by physical vapor transport (PVT). The structural, surface, and optical properties of m-plane and c-plane AlN crystals were comparatively studied by using high-resolution X-ray diffraction (HR-XRD), X-ray photoelectron spectroscopy (XPS), and Raman spectroscopy. Temperature-dependent Raman measurements showed that the Raman shift and the full width at half maximum (FWHM) of the E2 (high) phonon mode of the m-plane AlN crystal were larger than those of the c-plane AlN crystal, which would be correlated with the residual stress and defects in the AlN samples, respectively. Moreover, the phonon lifetime of the Raman-active modes largely decayed and its line width gradually broadened with the increase in temperature. The phonon lifetime of the Raman TO-phonon mode was changed less than that of the LO-phonon mode with temperature in the two crystals. It should be noted that the influence of inhomogeneous impurity phonon scattering on the phonon lifetime and the contribution to the Raman shift came from thermal expansion at a higher temperature. In addition, the trend of stress with increasing 1000/temperature was similar for the two AlN samples. As the temperature increased from 80 K to ~870 K, there was a temperature at which the biaxial stress of the samples transformed from compressive to tensile stress, while their certain temperature was different.

9.
J Phys Chem Lett ; 12(22): 5332-5338, 2021 Jun 10.
Artículo en Inglés | MEDLINE | ID: mdl-34062057

RESUMEN

Broad impact in the research community may be anticipated when a material's properties are capable of being manipulated artificially. Such a possibility has been explored here in the FAPbI3 perovskite structure of perovskite solar cells, which involves undesirable phase transition at working temperature, despite many attempts to resolve the issue. Essential steps have been taken here toward solving this problem by adopting an opposite strategy to incorporate the water molecules into the perovskite structure under the current materials framework by new structural physics maneuvering. The secondary bonding of the perovskite structure has been relocated, which altered the microstructure to remove the internal strain that caused the phase transition, resulting in not only a 10-fold enhancement in the moisture/structure stability but also a bandgap comparable to that of the favored α-FAPbI3. All this opens an unprecedented avenue in perovskite research, which will hopefully be of intrinsic interest to the broad materials research community as well.

10.
Sci Rep ; 11(1): 316, 2021 01 11.
Artículo en Inglés | MEDLINE | ID: mdl-33431976

RESUMEN

Maternal metabolism dysregulation during pregnancy predisposes offspring to major diseases, including hypertension, in later life, but the mechanism involved remains to be fully elucidated. A high-fat-diet (HFD) pregnant rat model was used to investigate whether excessive intrauterine lipid exposure was associated with elevated blood pressure in offspring and increased levels of leptin, an important biomarker and mediator of vascular dysfunction and hypertension. We found that gestational hyperlipidemia predisposed offspring to blood pressure elevation and sustained increases in leptin levels with no difference in body weight in the rat model. Increased leptin expression and leptin promoter hypomethylation were found in adipose tissues of HFD-exposed offspring. The treatment of mesenchymal stem cells with free fatty acids during adipogenic differentiation resulted in increased leptin expression, accompanied by leptin promoter hypomethylation. In addition, we also followed up 121 children to evaluate the association between maternal triglyceride levels and offspring blood pressure. Consistent with the animal study results, we observed elevated serum leptin levels and blood pressure in the offspring born to women with gestational hypertriglyceridemia. Our findings provide new insights that maternal hyperlipidemia is associated with elevated blood pressure in offspring and is associated with increases in leptin levels through epigenetic memory.


Asunto(s)
Presión Sanguínea/genética , Dieta Alta en Grasa/efectos adversos , Epigénesis Genética , Leptina/metabolismo , Exposición Materna/efectos adversos , Efectos Tardíos de la Exposición Prenatal/genética , Efectos Tardíos de la Exposición Prenatal/metabolismo , Adiponectina/metabolismo , Animales , Glucemia/metabolismo , Peso Corporal , Femenino , Insulina/metabolismo , Leptina/genética , Masculino , Embarazo , Efectos Tardíos de la Exposición Prenatal/fisiopatología , Ratas , Triglicéridos/sangre
11.
Langmuir ; 26(11): 7686-9, 2010 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-20459112

RESUMEN

Identifying the two distinctive wetting states on a nanostructured surface remains an open challenge. We report here a direct method of tracing the Wenzel and Cassie states by using Ag nanoparticles as tracing agents. The method provides an answer to the long-standing question of whether there is a transition from the Wenzel to Cassie state in the sliding angle drop on superhydrophobic nanocomposite thin films containing multiwalled carbon nanotubes.

12.
Zhonghua Yi Xue Za Zhi ; 90(1): 28-32, 2010 Jan 05.
Artículo en Zh | MEDLINE | ID: mdl-20356521

RESUMEN

OBJECTIVE: To investigation the effect of ropivacaine on the contraction of the isolated human umbilical artery and the mechanisms involved. METHODS: Endothelium-denuded human umbilical artery rings obtained from healthy full-term parturients were prepared. Using isometric force transducers and a fluorometer, the effect of ropivacaine in cumulative concentration on the contraction response induced by KCl in the presence or absence of verapamil, or verapamil plus ruthenium red or verapamil plus heparin was observed. Furthermore, the effect of ropivacaine on the contraction response of the artery rings incubated in different concentrations of extracellular Ca(2+) was also observed. RESULTS: Ropivacaine induced a dose-dependent biphasic contractile response of human umbilical artery rings: increasing at concentrations of 1.0 x 10(-5) to 1.0 x 10(-4) mol/L and decreasing from 3.0 x 10(-4) to 3.0 x 10(-3) mol/L, which was inhibited by verapamil, or verapamil plus ruthenium red, or verapamil plus heparin. No difference was found between pre-treatment of verapamil, verapamil plus ruthenium red and verapamil plus heparin. Ropivacaine induced no contractile response in Ca(2+)-free solution and a extracellular Ca(2+) dose-dependent increasing contractile response (1.0 x 10(-4) to 3.0 x 10(-2) mol/L). CONCLUSION: Ropivacaine induced a dose-dependent biphasic contractile response of human umbilical artery rings. The increase in intracellular Ca(2+) concentrations by the extracellular Ca(2+) influx, not by the release from the sarcoplasmic reticulum, is involved in ropivacaine-induced vasoconstriction of human umbilical artery smooth muscle.


Asunto(s)
Amidas/farmacología , Arterias Umbilicales/efectos de los fármacos , Vasoconstricción/efectos de los fármacos , Calcio/metabolismo , Relación Dosis-Respuesta a Droga , Humanos , Técnicas In Vitro , Ropivacaína , Arterias Umbilicales/metabolismo
13.
Medicine (Baltimore) ; 99(51): e23754, 2020 Dec 18.
Artículo en Inglés | MEDLINE | ID: mdl-33371135

RESUMEN

BACKGROUND: Insomnia with high incidence is usually accompanied by many other diseases, especially mental disorders with the under-diagnosis and under-treatment. Some studies demonstrated that acupuncture may be effective for emotional disorders accompanied by insomnia. The systematic review protocol is designed to guiding analysis the effectiveness and safety of acupuncture for emotional disorders in patients with insomnia. METHODS: Seven databases, Cochrane central register of controlled trials, Medline, Embase, China National Knowledge Infrastructure, Chinese Biomedical Literature database, VIP database and Wanfang database, will be searched from initial to December 2020. Randomized controlled trials (RCTs) of acupuncture for insomnia with emotional disorders (depression and anxiety) outcomes, which were reported in Chinese or English, will be included. The primary outcome is the change of degree of anxiety and depression. Study selection, data extraction and assessment of the risk of bias will be performed independently by 2 or more reviewers. Available data will be synthesized and statistically analyzed in RevMan V.5.3. The model of fixed effects will be used for the pooled data when the heterogeneity tests show little or no statistical heterogeneity (I2 < 50%). The random-effects model will be taken with heterogeneous data (50% ≤ I2 < 75%). RESULTS: The effect of acupuncture on emotional disorders in patients with insomnia will be assessed on Hamilton anxiety Scale, Hamilton anxiety Scale, Pittsburgh Sleep Quality Index, Insomnia Severity Index, Self-rating Anxiety Scale, Self-rating Depressive Scale and the number of participants secede and the number of patients reported adverse events. CONCLUSION: the emotional disorders interaction with insomnia and the increase of risk on disease evolving and insomnia-related burden, it is so momentous to know that the role of insomnia treatment on comorbidities. We should concern about the management of emotional disorders when treat insomnia, and acupuncture treatment anxiety and depression caused by insomnia may be effective. ETHICS AND DISSEMINATION: Ethics approval is not be needed because the data will not contain individual patient data, and there are no concerns about privacy. The results of this meta-analysis will be disseminated through publication in a peer-reviewed academic journal or relevant conference. INPLASY REGISTRATION NUMBER: INPLASY2020100115.


Asunto(s)
Terapia por Acupuntura/métodos , Trastornos de Ansiedad/terapia , Trastorno Depresivo/terapia , Trastornos del Inicio y del Mantenimiento del Sueño/terapia , Terapia por Acupuntura/efectos adversos , Trastornos de Ansiedad/epidemiología , Trastorno Depresivo/epidemiología , Humanos , Ensayos Clínicos Controlados Aleatorios como Asunto , Proyectos de Investigación , Índice de Severidad de la Enfermedad , Sueño , Trastornos del Inicio y del Mantenimiento del Sueño/epidemiología , Metaanálisis como Asunto
14.
Neural Netw ; 128: 107-125, 2020 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-32446189

RESUMEN

As a major step forward in machine learning, generative adversarial networks (GANs) employ the Wasserstein distance as a metric between the generative distribution and target data distribution, and thus can be viewed as optimal transport (OT) problems to reflect the underlying geometry of the probability distribution. However, the unequal dimensions between the source random distribution and the target data, result in often instability in the training processes, and lack of diversity in the generative images. To resolve the challenges, we propose here a multiple-projection approach, to project the source and target probability measures into multiple different low-dimensional subspaces. Moreover, we show that the original problem can be transformed into a variant multi-marginal OT problem, and we provide the explicit properties of the solutions. In addition, we employ parameterized approximation for the objective, and study the corresponding differentiability and convergence properties, ensuring that the problem can indeed be computed.


Asunto(s)
Aprendizaje Automático , Procesamiento de Imagen Asistido por Computador/métodos , Probabilidad
15.
ACS Appl Mater Interfaces ; 12(43): 48882-48889, 2020 Oct 28.
Artículo en Inglés | MEDLINE | ID: mdl-33054159

RESUMEN

The degradation of the perovskite solar cell structure was expected recently to be reversible, which opened a new gate to the enhancement of the device lifetime by reversing the process. However, the kinetic details of the structural collapse and recovery are still missing, without which the perovskite reversibility cannot be further explored. Due to the experimental difficulty, a purposeful numerical model was conducted in this report, to simulate the water diffusion process in the perovskite structure in both directions. It was found that the moisture diffusion needs to be initiated by a certain level of structural imperfection and is non-Fickian, as assisted by the collapse of the perovskite into the 1D chains. The reversibility was verified by the back diffusion, but accompanied by hysteresis, stagnancy, and even surprising instability, which initiated the water flow under initial equilibrium, due possibly to the imbalance during the reconstruction of the perovskite lattice. These observations offer new insights to form strategies of improvement, for example, via the possible self-healing perovskite devices.

16.
J Mol Cell Biol ; 12(1): 71-83, 2020 01 22.
Artículo en Inglés | MEDLINE | ID: mdl-31065688

RESUMEN

Basonuclin (BNC1) is expressed primarily in proliferative keratinocytes and gametogenic cells. However, its roles in spermatogenesis and testicular aging were not clear. Previously we discovered a heterozygous BNC1 truncation mutation in a premature ovarian insufficiency pedigree. In this study, we found that male mice carrying the truncation mutation exhibited progressively fertility loss and testicular premature aging. Genome-wide expression profiling and direct binding studies (by chromatin immunoprecipitation sequencing) with BNC1 in mouse testis identified several spermatogenesis-specific gene promoters targeted by BNC1 including kelch-like family member 10 (Klhl10), testis expressed 14 (Tex14), and spermatogenesis and centriole associated 1 (Spatc1). Moreover, biochemical analysis showed that BNC1 was associated with TATA-box binding protein-associated factor 7 like (TAF7L), a germ cell-specific paralogue of the transcription factor IID subunit TAF7, both in vitro and in testis, suggesting that BNC1 might directly cooperate with TAF7L to regulate spermatogenesis. The truncation mutation disabled nuclear translocation of the BNC1/TAF7L complex, thus, disturbing expression of related genes and leading to testicular premature aging. Similarly, expressions of BNC1, TAF7L, Y-box-binding protein 2 (YBX2), outer dense fiber of sperm tails 1 (ODF1), and glyceraldehyde-3-phosphate dehydrogenase, spermatogenic (GAPDHS) were significantly decreased in the testis of men with non-obstructive azoospermia. The present study adds to the understanding of the physiology of male reproductive aging and the mechanism of spermatogenic failure in infertile men.


Asunto(s)
Envejecimiento Prematuro/metabolismo , Azoospermia/metabolismo , Proteínas de Unión al ADN/deficiencia , Proteínas de Unión al ADN/metabolismo , Espermatogénesis/genética , Factores Asociados con la Proteína de Unión a TATA/metabolismo , Testículo/metabolismo , Factor de Transcripción TFIID/metabolismo , Factores de Transcripción/deficiencia , Factores de Transcripción/metabolismo , Envejecimiento Prematuro/genética , Animales , Azoospermia/genética , Azoospermia/patología , Proteínas de Unión al ADN/genética , Células HEK293 , Humanos , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Mutación , Transducción de Señal/genética , Factores Asociados con la Proteína de Unión a TATA/genética , Factor de Transcripción TFIID/genética , Factores de Transcripción/genética , Transfección
17.
J Phys Chem Lett ; 10(22): 7245-7250, 2019 Nov 21.
Artículo en Inglés | MEDLINE | ID: mdl-31689109

RESUMEN

The moisture instability of organic-inorganic hybrid perovskite solar cells has been a major obstacle to the commercialization, calling for mechanistic understanding of the degradation process, which has been under debate. Here we present a surprising discovery that the degradation is actually reversible, via in situ observation of X-ray diffraction, supported by FTIR and SEM. To isolate the hydrogen bond effect, water was replaced by methanol during the in situ experiment, revealing the decomposition to be initiated by the breakdown of N-H-I hydrogen bonds. This is followed by the step of organic iodide hydrolyzing, which can be inhibited in the neutral environment, making the whole process reversible under variable pH.

18.
Chem Commun (Camb) ; 55(22): 3251-3253, 2019 Mar 12.
Artículo en Inglés | MEDLINE | ID: mdl-30810121

RESUMEN

The crystal structure of a new perovskite material, (C4H8NH2)PbI3 was determined and illustrated by single crystal X-ray diffraction. UV spectra, photoluminescence and XRD results show it is a promising alternative to hybrid organic-inorganic perovskites due to it's good water resistance and suitable bandgap.

19.
Talanta ; 197: 211-217, 2019 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-30771926

RESUMEN

To fight against diabetes mellitus, from which more than 400 million people suffer in the world, the patients have to puncture their fingers 4-5 times a day for the blood glucose level checks when using a glucometer, causing invasive pain and the risk of infection. Therefore, non-invasive method has been urged for blood glucose monitoring, among which the mid-infrared spectroscopy (Mid-IR) response of interstitial fluid was found to be promising. However, despite the prolonged effort, the accuracy still falls below the FDA's requirement. To break this barrier which lasted for almost three decades, we discovered the finger contact pressure playing a critical role during the measurement, where the Mid-IR reading could be affected significantly by a small change of the finger posture. In addition, the Mid-IR absorption level was also found to be highly associated with individual, revealing the necessity of adjusting the calibration correlation for each patient. By imposing a certain contact pressure monitored by a pressure transducer, we were able to achieve over 95% certainty from the Mid-IR measurement of glucose concentration and 100% comparability to the "true" glucose concentration for the first time, which was mainly attributed to the morphological change of finger tissue under pressure. The previous works resulted in only about 70% accuracy on average, barely hitting 80 + %, whereas ours reaches 95%, finally exceeding the requirement of FDA.


Asunto(s)
Automonitorización de la Glucosa Sanguínea , Glucemia/análisis , Diabetes Mellitus/diagnóstico , Humanos , Presión , Espectrofotometría Infrarroja
20.
Medicine (Baltimore) ; 97(36): e12232, 2018 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-30200149

RESUMEN

Gestational diabetes mellitus (GDM) increases many health risks in offspring. The study aims to investigate the underlying mechanism in fetal risk of GDM.We collected maternal peripheral plasma and umbilical venous plasma samples from 4 GDM and 4 control patients during their delivery at a university-based women's hospital. An isobaric tag for relative and absolute quantitation-labeled proteomics analysis was performed. The enzyme-linked immunosorbent assay was used to confirm the change of cholesteryl ester transfer protein (CETP). Bioinformatic analysis was performed with Ingenuity Pathway Analysis (IPA) software package.We identified 19 up-regulated proteins and 15 down-regulated proteins in GDM peripheral plasma, 29 up-regulated proteins and 69 down-regulated proteins in GDM umbilical venous plasma. CETP concentration was significantly lower in both GDM peripheral plasma and umbilical venous plasma. Upstream regulator analysis predicted follicle-stimulating hormone (FSH) as the activated regulator of differentially expressed proteins.The protein profiles in both GDM peripheral plasma and umbilical venous plasma between normal and GDM patients were significantly different. The results indicated that CETP and FSH might associates with health problem of GDM offspring.


Asunto(s)
Diabetes Gestacional/sangre , Sangre Fetal/metabolismo , Plasma/metabolismo , Proteoma , Adulto , Cromatografía , Biología Computacional , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Embarazo , Proteómica , Espectrometría de Masa por Ionización de Electrospray , Espectrometría de Masas en Tándem
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA