RESUMEN
PURPOSE: This study was conducted to evaluate the safety, tolerability, pharmacokinetics and pharmacodynamics of dexlansoprazole injection in healthy subjects. METHODS: Dexlansoprazole (20-90 mg) or lansoprazole (30 mg) was administrated intravenously to healthy male and female volunteers. All the subjects were sampled for pharmacokinetic (PK) analysis and 64 of them were monitored for 24-h intragastric pH prior to and after administration in the pharmacodynamic (PD) study. RESULTS: Maximum plasma concentration (Cmax) and area under the concentration-time curve (AUC0-τ) for dexlansoprazole injection was dose-proportional over the range of 20-90 mg following a single intravenous administration. Total clearance and half-life (t1/2) was independent of dose, and ranged from 4.69 L/h to 5.85 L/h and from 1.24 h to 2.17 h, respectively. A single dose of dexlansoprazole (30 mg) resulted in higher gastric pH compared to that of lansoprazole, evidenced by a mean 24-h gastric pH of 6.1 ± 1.2 (lansoprazole: 5.4 ± 1.1) and 24-h gastric pH > 6 post drug dose holding time of 64.2 ± 21.0% (lansoprazole: 49.5 ± 21.5%). CONCLUSION: Dexlansoprazole injection was safe and well tolerated for up to 5-day repeated intravenous administration dose of 30 mg. The recommended dosage for dexlansoprazole injection is 30 mg for an adequate gastric acid control.
Asunto(s)
Dexlansoprazol/farmacología , Inhibidores de la Bomba de Protones/farmacología , Área Bajo la Curva , China , Dexlansoprazol/administración & dosificación , Dexlansoprazol/efectos adversos , Dexlansoprazol/farmacocinética , Femenino , Jugo Gástrico/química , Humanos , Concentración de Iones de Hidrógeno , Masculino , Inhibidores de la Bomba de Protones/administración & dosificación , Inhibidores de la Bomba de Protones/efectos adversos , Inhibidores de la Bomba de Protones/farmacocinéticaRESUMEN
The occurrence and distribution of eight selected endocrine-disrupting chemicals were investigated in samples of surface water and suspended particulate matter (SPM) in Nanjing section of Yangtze River over a year (the flow period, the wet period and the dry period). All target compounds were detected at least once in surface water with 4-tert-butylphenol (4-TBP), nonyphenol (NP) and bisphenol A (BPA) as the dominant compounds, with concentrations in the range of 225-1121ng/L, 1.4-858ng/L and 1.7-563ng/L, respectively. Except for December, all selected compounds for the other sampling times were not found in all sampling points. NP (mean concentration 69.8µg/g) and BPA (mean concentration 51.8µg/g) were also the dominant estrogens in SPM. In addition, the highest total compounds concentrations were found in December in both phases, which could be due to the low flow conditions and temperature during this season. Meanwhile, a significant positive correlation was found between the total compounds concentrations in the water phase and those in SPM phase. Risk assessment based on the calculated risk quotients (RQ) showed that low and moderate risk for the aquatic environment from presence of the target compounds at all sampling points with exception of 4-TBP and NP which might pose a high risk to aquatic organisms.
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Disruptores Endocrinos/análisis , Material Particulado/análisis , Ríos/química , Contaminantes Químicos del Agua/análisis , Organismos Acuáticos , Compuestos de Bencidrilo/análisis , China , Monitoreo del Ambiente , Estrógenos/análisis , Fenoles/análisis , Medición de RiesgoRESUMEN
In this study, tri-functional immunofluorescent probes (Ce6-IgG-QDs) based on covalent combinations of quantum dots (QDs), immunoglobulin G (IgG) and chlorin e6 (Ce6) were developed and their photodynamic ability to induce the death of cancer cells was demonstrated. Strategically, one type of second-generation photosensitizer, Ce6, was first coupled with anti-IgG antibody using the EDC/NHS cross-linking method to construct the photosensitive immunoconjugate Ce6-IgG. Then, a complex of Ce6-IgG-QDs immunofluorescent probes was obtained in succession by covalently coupling Ce6-IgG to water soluble CdTe QDs. The as-manufactured Ce6-IgG-QDs maintained the bio-activities of both the antigen-antibody-based tumour targeting effects of IgG and the photodynamic-related anticancer activities of Ce6. By way of polyclonal antibody interaction with rabbit anti-human epidermal growth factor receptor (anti-EGFR antibody, N-terminus), Ce6-IgG-QDs were labelled indirectly onto the surface of human hepatocarcinoma (HepG2) cells in cell recognition and killing experiments. The results indicated that the Ce6-IgG-QDs probes have excellent tumour cell selectivity and higher photosensitivity in photodynamic therapy (PDT) compared with Ce6 alone, due to their antibody-based specific recognition and location of HepG2 cells and the photodynamic effects of Ce6 killed cells based on efficient fluorescence resonance energy transfer between QDs and Ce6. Copyright © 2015 John Wiley & Sons, Ltd.
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Antineoplásicos/farmacología , Colorantes Fluorescentes/farmacología , Inmunoglobulina G/química , Fármacos Fotosensibilizantes/farmacología , Porfirinas/farmacología , Puntos Cuánticos , Antineoplásicos/síntesis química , Antineoplásicos/química , Muerte Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Clorofilidas , Relación Dosis-Respuesta a Droga , Ensayos de Selección de Medicamentos Antitumorales , Colorantes Fluorescentes/síntesis química , Colorantes Fluorescentes/química , Células Hep G2 , Humanos , Fármacos Fotosensibilizantes/síntesis química , Fármacos Fotosensibilizantes/química , Porfirinas/química , Relación Estructura-ActividadRESUMEN
In this work, fluorescent Bacillus subtilis (B. subtilis) cells were developed as probes for imaging applications and to explore behaviorial interaction between B. subtilis and Staphylococcus aureus (S. aureus). A novel biological strategy of coupling intracellular biochemical reactions for controllable biosynthesis of CdSe quantum dots by living B. subtilis cells was demonstrated, through which highly luminant and photostable fluorescent B. subtilis cells were achieved with good uniformity. With the help of the obtained fluorescent B. subtilis cells probes, S. aureus cells responded to co-cultured B. subtilis and to aggregate. The degree of aggregation was calculated and nonlinearly fitted to a polynomial model. Systematic investigations of their interactions implied that B. subtilis cells inhibit the growth of neighboring S. aureus cells, and this inhibition was affected by both the growth stage and the amount of surrounding B. subtilis cells. Compared to traditional methods of studying bacterial interaction between two species, such as solid culture medium colony observation and imaging mass spectrometry detection, the procedures were more simple, vivid, and photostable due to the efficient fluorescence intralabeling with less influence on the cells' surface, which might provide a new paradigm for future visualization of microbial behavior.
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Bacillus subtilis/metabolismo , Compuestos de Cadmio/metabolismo , Colorantes Fluorescentes/metabolismo , Interacciones Microbianas , Puntos Cuánticos , Compuestos de Selenio/metabolismo , Bacillus subtilis/fisiología , Adhesión Bacteriana , Transducción de Señal , Coloración y Etiquetado/métodos , Staphylococcus aureus/fisiologíaRESUMEN
The related substances in docetaxel injection were identified by LC-MS/MS. Ethyl acetate was used to extract the injection to remove the pharmaceutical excipients. HPLC separation was carried out on a Hedera ODS-2 column (150 mm x 2.1 mm, 5 microm) with a mobile phase consisting of acetonitrile - 0.1% acetate acid aqueous solution (40: 60). Electrospray ionization source was set in the positive mode for the LC-ESI-MS/MS, and the ion monitoring modes were full scan and product ion scan. According to the mass spectra of the related substances, the fragment profiles were explained, and the chemical structures were elucidated. Docetaxel and its main related substances were well separated. Nine related substances in docetaxel injection were detected by LC-MS/MS. Their chemical structures were proposed, and four of them were identified in the docetaxel injection for the first time. The established LC-MS/MS method is effective in the separation and identification of the related substances in docetaxel injection. The test results are useful for its quality control.
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Antineoplásicos/química , Taxoides/química , Antineoplásicos/administración & dosificación , Antineoplásicos/análisis , Cromatografía Líquida de Alta Presión , Docetaxel , Contaminación de Medicamentos , Inyecciones , Peso Molecular , Control de Calidad , Espectrometría de Masa por Ionización de Electrospray , Espectrometría de Masas en Tándem , Taxoides/administración & dosificación , Taxoides/análisisRESUMEN
Aqueous thiol-capped CdSe QDs with a narrow, symmetric emission were prepared under a low temperature. Based on the fluorescence enhancement of thiol-stabilized CdSe quantum dots (QDs) caused by edaravone, a simple, rapid and specific quantitative method was proposed to the edaravone determination. The concentration dependence of fluorescence intensity followed the binding of edaravone to surface of the thiol-capped CdSe QDs was effectively described by a modified Langmuir-type binding isotherm. Factors affecting the fluorescence detection for edaravone with thiol-stabilized CdSe QDs were studied, such as the effect of pH, reaction time, the concentration of CdSe QDs and so on. Under the optimal conditions, the calibration plot of C/(I-I(0)) with concentration of edaravone was linear in the range of (1.45-17.42) microg/mL (0.008-0.1 micromol/L) with correlation coefficient of 0.998. The limit of detection (LOD) (3sigma/kappa) was 0.15 microg/mL (0.0009 micromol/mL). Possible interaction mechanism was discussed.
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Bioensayo/métodos , Cadmio/química , Depuradores de Radicales Libres/análisis , Puntos Cuánticos , Selenio/química , Antipirina/análogos & derivados , Antipirina/análisis , Edaravona , Fluorescencia , Espectrometría de Fluorescencia/instrumentación , Espectrometría de Fluorescencia/métodos , Agua/químicaRESUMEN
A novel assay of Norfloxacin with a sensitivity at the microgram level is proposed based on the measurement of enhanced fluorescence intensity signals by the interaction of functionalized nano-CdS with Norfloxacin. The CdS nanoparticles were synthesized by thioacetamide (TAA) and cadmium nitrate (Cd(NO3)2) in the alkaline solution, and the nanoparticles proved to be stable in the aqueous solution. At pH 7.4, the fluorescence signals of functionalized nano-CdS were greatly enhanced by Norfloxacin in the region of 300-700 nm characterized by the peak around 495 nm, and the surface defect-related emission located at 565 nm. However, the surface defect-related emission was unconspicuous, thus we concluded that the functionalized nano-CdS QDs (Quantum Dots) possessed excellent luminescence capability and favourable structure. At the same time, the absorption spectra and transmission electron microscopy (TEM) also proved this deduction. The external reaction conditions (such as effect of buffer system, pH, ionic strength, reaction time and temperature, colloid concentration) were discussed. The result showed that better fluorescence signals could be obtained in the condition of 0.10 mol x L(-1) Tris-HCl, pH 7.4, 0.1 mol x L(-1) NaCl and the reaction time 5 min. The fluorescence emission spectra of CdS QDs with increasing Norfloxacin concentration were recorded under the optimal condition. From the fluorescence intensity and peak position of nano-CdS colloidal as different concentration of Norfloxacin was added, the possible mechanism of reaction between mercapto-acetic acid capped CdS and Norfloxacin was discussed. Linear relationship can be established between the enhanced fluorescence intensity and Norfloxacin concentration in the range of 1.25-11.25 microg x mL(-1) (3.92-35.27 micromol x L(-1)) or 11.25-100.0 microg x mL(-1) (35.27-313.5 micromol x L(-1)). The limit of detection is 1.5 x 10(-3) x microg x mL(-1), which can be applied to the determination of blood serum samples. Based on this, a new direct quantitative determination method for Norfloxacin in synthetic samples without separation of foreign substances is established. At the same time, the possible enhancing mechanism is due to the formation of exciplex during reaction between nano-CdS and Norfloxacin, providing a guidance for the study of pharmacokinetics.
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Compuestos de Cadmio , Nanopartículas , Norfloxacino/química , Sulfatos , Coloides , Concentración de Iones de Hidrógeno , Microscopía Electrónica de Transmisión , Concentración Osmolar , Puntos Cuánticos , Espectrometría de Fluorescencia , TemperaturaRESUMEN
Macrophages eliminate and destroy invading bacteria and contaminants by engulfing them or secreting cytokines that trigger downstream immune responses. Consequently, impairment of the phagocytic functions of macrophages and/or suppressing their cytokine secretion are dangerous to organisms that rely on immune protection. Accordingly, exposure to environmental nanoparticles (NPs) that display immunomodulatory properties are serious. In this work, two types of NPs, i.e., mild-toxicity CuInS2 NPs and high-toxicity CdTe NPs, were used to evaluate the effects of NP exposure for macrophages. Following incubation for 24 h, THP-1-derived macrophage viability was assessed using an MTT method after exposing the THP-1 cells to different concentrations of CuInS2 or CdTe NPs. Phagocytosis assays demonstrated that both CuInS2 and CdTe NPs impair phagocytic activity toward Staphylococcus aureus (S. aureus). After pretreatment with CuInS2 and CdTe NPs at 4 µmol/L, THP-1 macrophages exhibited decreases in phagocytic ratio from ca. 32.9% to ca. 18.5% and 18.7%, respectively. Since the zeta potentials of intact and weathered CuInS2 NPs were distributed over a wide range from positive to negative, large quantities of intact and weathered CuInS2 NPs bore sufficient positive charge on their surfaces to induce membrane depolarization, thus theoretically providing electrostatic forces between S. aureus and THP-1, which could induce downstream intracellular events that increase phagocytosis. However, real time polymerase chain reaction arrays revealed that transcription of the pro-inflammatory factors IL-1ß, IL-6, and TNF-α decreased while that of the anti-inflammatory factor IL-10 increased after treatment with CuInS2 NPs. Furthermore, transcription of TNF-α decreased while IL-10 increased after treatment with CdTe NPs. Thus, both kinds of NPs inhibited phagocytosis of S. aureus by THP-1 to some extent, confirming that immunosuppression can occur when macrophages are exposed to environmental NPs.
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Citocinas/metabolismo , Factores Inmunológicos/metabolismo , Macrófagos/efectos de los fármacos , Nanopartículas/metabolismo , Fagocitosis/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Humanos , Factores Inmunológicos/química , Macrófagos/metabolismo , Nanopartículas/química , Staphylococcus aureus/inmunología , Células THP-1RESUMEN
A simple biological strategy to couple intracellular irrelated biochemical reactions of staphylococcus aureus CMCC 26003 (S. aureus) with inorganic metal ions to synthesize cadmium selenide quantum dots (CdSe QDs) was demonstrated. Correspondingly, S. aureus as living matrices are internally generated and labeled with fluorescent QDs by the smart strategy. Several key factors in the process of biosynthesis were systematically evaluated. At the same time, ultraviolet-visible (UV-Vis), photo-luminescence (PL), inverted fluorescence microscopy and transmission electron microscopy (TEM) were utilized to study the characters of the as produced CdSe QDs. In addition, cytotoxicity and photostability of the QDs containing bacteria were also tested and evaluated as a whole. The results showed that intracellular CdSe nanocrystals had successfully formed in S. aureus living cells, which were less toxic, highly fluorescent and photostable. These fluorescent S. aureus bacteria were next applied as invading pathogens as well as fluorescent bioprobes for exploring the phagocytic behavior of THP-1-derived macrophage. Results proved that internal CdSe QDs labeling had no significantly adverse effects compared with the kind of infection reference, fluorescein isothiocyanate (FITC) stained S. aureus pathogen. Assuredly, the methods presented here provide researchers with a useful option to analyze the behavior of S. aureus as a type of infectious pathogen, which would also help understand the complex interplay between host cells and the invading bacteria on molecular level.
RESUMEN
A novel assay of ciprofloxacin with a sensitivity at the microgram level is proposed based on the measurement of enhanced fluorescence intensity signals resulting from the interaction of functionalized nano-CdS with ciprofloxacin. The CdS nanoparticles was synthesized by thioacetamide (TAA) and cadmium nitrate (Cd(NO(3))(2)) in the alkaline solution. At pH 7.4, the fluorescence signals of functionalized nano-CdS were greatly enhanced by ciprofloxacin with the increase concentration of ciprofloxacin. Linear relationship can be established between the enhanced fluorescence intensity and ciprofloxacin concentration in the range of (1.25-8.75)x10(-4) mg mL(-1) ((3.77-26.4) x 10(-4)mmol L(-1)) or (8.75-1200) x 10(-4)mg mL(-1) ((26.4-3625) x 10(-4) mmol L(-1)). The limit of detection is 7.64 x 10(-6) mg mL(-1) (2.31 x 10(-5)mmol L(-1)). Based on this, a new direct quantitative determination method for ciprofloxacin in human serum samples without separation of foreign substances was established. The contents of ciprofloxacin in human serum samples were determined with recovery of 95-105% and relative standard deviation (R.S.D.) of 1.5-2.5%. This method was proved to be very sensitive, rapid, simple and tolerance of most interfering substances.
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Compuestos de Cadmio/química , Ciprofloxacina/química , Sulfuros/química , Tampones (Química) , Compuestos de Cadmio/análisis , Calibración , Técnicas de Química Analítica/métodos , Ciprofloxacina/análisis , Coloides/química , Colorantes Fluorescentes/farmacología , Humanos , Concentración de Iones de Hidrógeno , Iones , Microscopía Electrónica de Transmisión , Nanopartículas/química , Reproducibilidad de los Resultados , Espectrometría de Fluorescencia/métodos , Sulfuros/análisis , TemperaturaRESUMEN
The interaction of sorbitol and bovine serum album (BSA) was studied by fluorescence and ultraviolet absorption spectra. As it is well known to us, the interactional analysis between small molecular drug and biology macromolecule (such as protein, DNA, etc) is one of the important interactional analyses, which can not only offer new biological view but also supply chance for chemist and biochemist to synthesize new drug capable of regulating the biology process effectively. In the present paper, fluorescence spectrophotometry was first employed to study the interaction between BSA and sorbitol. At the same time, the synchronous fluorescence spectroscopy was adopted to review the configuration of BSA influenced by sorbitol, which provides important significance for clinical medication. The results show that sorbitol has strongly quenched the fluorescence of bovine serum albumin in natural physiological condition, the quenching mechanism is a static quenching procedure at different temperatures and drug concentration, and the variational absorption spectra also proves this deduction. At the same time, this article has also examined the influences of sorbitol on the fluorescence quenching of bovine serum albumin at different temperatures and drug concentration. The binding constants and the number of binding sites between sorbitol and BSA were calculated at different temperatures. Furthermore, the enthalpy and entropy changes in the interaction of sorbitol and bovine serum album were also obtained by the equations of Stern-Volmer and Lineweaver-Burk. From the thermodynamic parameters, it can be judged that the primary binding power between sorbitol and BSA is electrostatic force. Moreover, the synchronous fluorescence spectroscopy was applied to examine the effect of sorbitol on the configuration of BSA. The alterative configuration of BSA may be induced by the hydrophobicity environment of tyrosine with the increase in drug concentration. In conclusion, the fluorescence method is highly sensitive and convenient in the study of intermolecular interaction. Further studies in this field will open up the way to applications of biology macromolecule in analytical chemistry and analytical biochemistry.
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Albúmina Sérica Bovina/química , Sorbitol/química , Espectrometría de Fluorescencia/métodos , Unión Proteica , Temperatura , TermodinámicaRESUMEN
CdS nanoparticles capped by mercaptoacetic acid have been successfully synthesized by microwave method employing thioacetamide as sulfur source, which was proved to be a simple, rapid and specific mothod compared with traditional synthetical methods, such as precipitation, sol-gel, solvo-thermal method and so on. The concrete procedure synthesizing CdS nanoparticles was as follows: Cd(NO3)2 (40 mL, 5 mmol c L(-1)) was titrated with mercaptoacetic acid to pH 2.0, resulting in a turbid blue solution. NaOH (0.1 mol x L(-1)) was then added dropwise until the pH was 7 and the solution was again colorless. While quickly stirring the solution, 40 mL of 5 mmol x L(-1) CH3CSNH2 was added. Subsequently, the solution was adjusted to pH 9.0 and placed in a microwave oven for 25 min with power 30% (it means that if microwave works in a 30 s regime, it works 6 s, and does not work 24 s. This is some kind of pulse regime, but the totalpower is still 100%). This kind of nanoparticles were water-soluble and symmetrical. The diameter of CdS nanoparticles which have a spherical morphology was determined to be 12 nm by transmission electron microscopy(TEM), which posess perfect uniforminty. According to literatures report, there are two kinds of emission peak: one is edge-emission peak, and the other is surface blemish emission. In contrast to edge-emission peak, the surface blemish emission shows red shift on fluorescence spectra. In the present paper, the prominent peak of CdS QDs fluorescence spectrum was located at 490 nm, the humpbacked peak caused by surface blemish of CdS nanoparticles was located at 565 nm. However, the surface blemish emission was unconspicuous, thus we can conclude that the synthetical CdS QDs possesses excellent luminescence capability and favorable structure. The size and absorption and fluorescence spectra of CdS nanoparticles at different microwave power, pH value, reaction time and different sulfur source were investigated. The result showed that the better nanoparticles could be obtained in the condition of 30% microwave power, pH 9.0 at the beginning of reaction, and the time of microwave reaction of 25 min. The synthesized nanoparticles were compared with the nanoparticles with CH3 CSNH2, NH2CSNH2 and Na2S as sulfur source. The experiment indicated that CdS nanoparticles applying CH3CSNH2 as sulfur source showed strong edge-emission, and blemish emission was weak, so the fluorescence quality is excellent; but CdS nanoparticles applying NH2CSNH2 as sulfur source showed weak edge-emission; and CdS nanoparticles applying Na2S as sulfur source showed mainly fluorescence blemish emission. At the same time, the mercaptoacetic acid capped CdS nanoparticles were employed to study the quantitative analysis of Cu2+. According to the results of experiment, in a certain range of concentration(6.4-512 microg x L(-1)), Cu2+ quenched the fluorescence intensity of mercaptoacetic acid capped CdS nanoparticles with good linearity, which can be used in the determination of trace Cu2+ in samples. In conclusion, this kind of method supplied a new way to study synthesizing the CdS nanoparticles.
RESUMEN
Bacillus amyloliquefaciens containing intracellularly biosynthesized cadmium selenide (CdSe) quantum dots (QDs) was used as a fluorescent bioprobe. Several parameters in the QD biosynthesis process were systematically optimized. The optimized protocol for producing high-quality CdSe QDs in B. amyloliquefaciens features mild synthetic conditions, good reproducibility, short reaction time and high yield. This process shows promise for the mass production of QDs by bacterial matrices. The resultant fluorescent B. amyloliquefaciens containing intracellular CdSe QDs was used as a bioprobe for the simple detection of copper (II) ions in blood plasma. The selective permeability of the bacterial cell membrane along with the protection provided by a protein envelope on the QD surface prevented interference by other components of blood plasma, resulting in the accurate determination of Cu2+. Using the copper addition method, the content of Cu2+ in human blood plasma samples was determined to be 15.6-18.5 µmol/L, consistent with atomic absorption spectroscopy results. The technique developed here shows potential for the simple determination of Cu2+ in plasma with excellent selectivity and good sensitivity.
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Bacillus amyloliquefaciens/metabolismo , Compuestos de Cadmio/química , Cobre/sangre , Colorantes Fluorescentes/química , Puntos Cuánticos , Compuestos de Selenio/química , Adulto , HumanosRESUMEN
Quantum dots (QDs) are semiconductor nanoparticles (NPs) that offer valuable functionality for cellular labeling, drug delivery, solar cells and quantum computation. In this study, we reported that CdSe QDs could be bio-synthesized in Bacillus licheniformis. After optimization, the obtained CdSe QDs exhibited a uniform particle size of 3.71±0.04nm with a maximum fluorescence emission wavelength at 550nm and the synthetical positive ratio can reach up to 87%. Spectral properties, constitution, particle sizes and crystalline phases of the CdSe QDs were systematically and integrally investigated. The CdSe QD-containing Bacillus licheniformis cells were further used as whole fluorescent bio-probes to detect copper (II) (Cu2+) in water, which demonstrated a low limit of detection (0.91µM). The assay also showed a good selectivity for Cu2+ over other ions including Al3+, Cd2+, Mg2+, K+, Na+, NH4+, Zn2+, CH3COO+, Pb2+ and I-. Our study suggests the fluorescent CdSe QDs-containing Bacillus licheniformis bio-probes as a promising approach for detection of Cu2+ in complex solution environment.
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Bacillus licheniformis/metabolismo , Compuestos de Cadmio , Cobre/análisis , Puntos Cuánticos/metabolismo , Puntos Cuánticos/microbiología , Compuestos de Selenio , Agua/análisis , Colorantes Fluorescentes/química , Límite de Detección , Puntos Cuánticos/ultraestructura , Espectrometría de Fluorescencia , Contaminantes Químicos del Agua/análisisRESUMEN
In the paper, a green and efficient biosynthetical technique was reported for preparing cadmium sulfide (CdS) quantum dots, in which Escherichia coli (E. coli) was chosen as a biomatrix. Fluorescence emission spectra and fluorescent microscopic photographs revealed that as-produced CdS quantum dots had an optimum fluorescence emission peak located at 470nm and emitted a blue-green fluorescence under ultraviolet excitation. After extracted from bacterial cells and located the nanocrystals' foci in vivo, the CdS quantum dots showed a uniform size distribution by transmission electron microscope. Through the systematical investigation of the biosynthetic conditions, including culture medium replacement, input time point of cadmium source, working concentrations of raw inorganic ions, and co-cultured time spans of bacteria and metal ions in the bio-manufacture, the results revealed that CdS quantum dots with the strongest fluorescence emission were successfully prepared when E. coli cells were in stationary phase, with the replacement of culture medium and following the incubation with 1.0×10-3mol/L cadmium source for 2 days. Results of antimicrobial susceptibility testing indicated that the sensitivities to eight types of antibiotics of E. coli were barely changed before and after CdS quantum dots were prepared in the mild temperature environment, though a slight fall of antibiotic resistance could be observed, suggesting hinted the proposed technique of producing quantum dots is a promising environmentally low-risk protocol.
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Compuestos de Cadmio/metabolismo , Escherichia coli/efectos de los fármacos , Escherichia coli/metabolismo , Puntos Cuánticos/química , Sulfuros/metabolismo , Farmacorresistencia Bacteriana , Escherichia coli/ultraestructura , Tecnología Química Verde , Pruebas de Sensibilidad Microbiana , Nanopartículas/química , Nanopartículas/ultraestructura , Puntos Cuánticos/ultraestructura , Espectrometría de FluorescenciaRESUMEN
In the present paper, a fluorescence method was used to study at different pH the fluorescence quenching of bovine serum albumin (BSA) by its interaction with balofloxacin (BLFX). The interaction association constants of BSA and BLFX were determined from a double reciprocal line Weaver-Burk plot. According to the Forster dipole-dipole energy transfer, the distance to be measured between the BLFX and tryptophane is 5.09 nm. From thermodynamical coordination it can be judged that the binding power between BLFX and BSA is electrostatic effect. The effect of BLFX on the conformation of BSA was also analyzed by using synchronous fluorescence spectroscopy.
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Fluorescencia , Fluoroquinolonas/química , Albúmina Sérica Bovina/química , Animales , Bovinos , Transferencia de Energía , Fluoroquinolonas/metabolismo , Cinética , Unión Proteica , Albúmina Sérica Bovina/metabolismo , Espectrometría de Fluorescencia , Espectrofotometría Ultravioleta , Electricidad Estática , TermodinámicaRESUMEN
In this work, nano-CdS was successfully prepared. The nano-CdS was also modified with L-cysteine. Absorption and fluorescence spectra of CdS nanoparticles for different pH values, reaction times and different Cys/Cd2+/S ratios were investigated. Meanwhile a fluorescence enhancing effect was observed between trace zinc ions and the functionalized L-Cys-CdS nanoparticles. The response is linearly proportional to the concentration of zinc ions from 1.0 to 15 micromol x L(-1). The functionalized nanoparticles are hopeful of use as fluorescence probes in detecting trace elements in biological samples.
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Compuestos de Cadmio/química , Cisteína/química , Nanopartículas/química , Espectrometría de Fluorescencia/métodos , Sulfuros/química , Compuestos de Cadmio/síntesis química , Colorantes Fluorescentes/química , Concentración de Iones de Hidrógeno , Cinética , Espectrofotometría Ultravioleta , Sulfuros/síntesis químicaRESUMEN
OBJECTIVE: To explore the ratio of lymphocyte subsets in peripheral blood of patients with aplastic anemia (AA) and patients with hypoplastic myelodysplastic syndrome (hypo-MDS) patients and to evaluate their significance. METHODS: The clinical data of 181 cases of AA and 111 cases of hypo-MDS from January 2008 to December 2014 were collected from Blood Diseases Hospital of Chinese academy of medical sciences, and then the differences of lymphocyte subsets and its effect in 2 groups were analyzed. RESULTS: CD4+/CD8+ ratio, proportion of CD3+ cells and its subsets CD3+CD4+/CD3+CD8+ cells in hypo-MDS group were not significant different from AA group (P>0.05). the proportion of CD3-CD16/CD56+ NK cells and CD3+CD57+T-LGL cells in hypo-MDS group was significantly higher than that in AA group (P<0.05, P<0.01), but CD19+ B lymphocyte percentage in hypo-MDS patients was lower than that in AA patients (P<0.05). After dividing group according to CD4+/CD8+ ratio, the ratios of CD3+ CD16/CD56+ NK cells and CD3+/CD57+ T-LGL cells were higher only in normal CD4+/CD8+ ratio group of hypo-MDS patients than those in AA patients, while the ratio of B lymphocytes was significant different in inverted CD4+/CD8+ ratio group between hypo-MDS and AA patients. The CD19+ B lymphocyte ratio in hypo-MDS patients was significantly lower than that in AA patients (P<0.05). As well, the levels of erythrocytes and platelets in peripheral blood between hypo-MDS and AA patients only in normal CD4+/CD8+ ratio group were significantly different, while the significant difference of WBC count and reticulocyte ratio were observed in high CD4+/CD8+ ratio and non-inverted CD4+/CD8+ ratio groups, respectively; the significant difference of bone marrow blast ratio and muture monocyte ratio was found in high CD4+/CD8+ ratio group. CONCLUSION: The changes of lymphocyte subsets can be used as an reference indicator for differential diagnosis of hypo-MDS and AA. The comparative analysis of patients with these 2 kinds of diseases after dividing into subgroups according to ratio of CD4+/CD8+ cells is beneficial to differentiat diagnosis.
Asunto(s)
Anemia Aplásica , Subgrupos Linfocitarios , Síndromes Mielodisplásicos , Linfocitos B , Médula Ósea , Relación CD4-CD8 , Humanos , Células Asesinas Naturales , Recuento de LinfocitosRESUMEN
A novel simple, fast and efficient supercritical fluid chromatography (SFC) method was developed and compared with RPLC method for the separation and determination of impurities in rifampicin. The separation was performed using a packed diol column and a mobile phase B (modifier) consisting of methanol with 0.1% ammonium formate (w/v) and 2% water (v/v). Overall satisfactory resolutions and peak shapes for rifampicin quinone (RQ), rifampicin (RF), rifamycin SV (RSV), rifampicin N-oxide (RNO) and 3-formylrifamycinSV (3-FR) were obtained by optimization of the chromatography system. With gradient elution of mobile phase, all of the impurities and the active were separated within 4 min. Taking full advantage of features of SFC (such as particular selectivity, non-sloping baseline in gradient elution, and without injection solvent effects), the method was successfully used for determination of impurities in rifampicin, with more impurity peaks detected, better resolution achieved and much less analysis time needed compared with conventional reversed-phase liquid chromatography (RPLC) methods.